Plant Circular RNA Database

Detail information of Os05g0149800_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os05g0149800_circ_g.3
					
						ID in PlantcircBase
						osa_circ_026587
					
						Alias
						Os_ciR4880
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 2848123-2849734  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os05g0149800						
					
						Parent gene annotation
						
							EF-Hand type domain containing protein. (Os05t0149800-01);Simila
r to Discordia 1. (Os05t0149800-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0149800_circ_g.2 Os05g0149800_circ_g.4 Os05g0149800_circ_g.5 						
					
						Support reads
						4/2
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0149800-02:6
Os05t0149800-01:6

General Information
CircRNA Name	Os05g0149800_circ_g.3
ID in PlantcircBase	osa_circ_026587
Alias	Os_ciR4880
Organism	Oryza sativa
Position	chr5: 2848123-2849734 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os05g0149800
Parent gene annotation	EF-Hand type domain containing protein. (Os05t0149800-01);Simila r to Discordia 1. (Os05t0149800-02)
Parent gene strand	+
Alternative splicing	Os05g0149800_circ_g.2 Os05g0149800_circ_g.4 Os05g0149800_circ_g.5
Support reads	4/2
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0149800-02:6 Os05t0149800-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATGTTTCTTGCACTTGACAAAGATACAAATGGAACATTGAGCAAACAGGAGCTCAAGGAATATG
CAGATGGCACATTGACTGATATCTTCATTGAAAGAGatgaattatgaagacttttgccatattg
caacagtatgcacagagcagatcggccagaagtgcaagcgtttcttcagcccttcaaattttat
gaagtttg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 607
Transcript exons: 2848123-2848281,2848817-2848888,2848964-2849096,2849210-
2849274,2849413-2849488,2849633-2849734
ATGAATTATGAAGACTTTTGCCATATTGCAACAGTATGCACAGAGCAGATCGGCCAGAAGTGCA
AGCGTTTCTTCAGCCCTTCAAATTTTATGAAGTTTGAGAAGGATGATTCTGGGAGAATTGCAAT
CCTACCATTTTATCTTTATGTTATGCGGACAGTATCTCTCACACAAGCGAGAATTGATATGAGT
GAGCTTGATGAGGATTCTGATGGTTTCCTTCAACCTCATGAAATGGAAGCATACATAAGAGGGC
TTATCCCCAATTTGGCACAACTGCGTGATATGCCATCAGCATTTGTTCAAATGTACTGCCGCAT
AGCTGCACGCAAGTTCTTTTTCTTCTGCGATCCACACAGACGAGGGAAAGCATGCATAAAGAAA
GTATTGCTGAGCAATTGTCTTCAGGAACTTATGGAACTACATCAGGAGAGTGAGGAAGAGGTTA
CTGATACTGAGCAGGCTGAAAATTGGTTTTCCCTGACGTCAGCTCAGCGCATTTGTGATATGTT
TCTTGCACTTGACAAAGATACAAATGGAACATTGAGCAAACAGGAGCTCAAGGAATATGCAGAT
GGCACATTGACTGATATCTTCATTGAAAGAG
						
					

						Genomic sequence
						
							ATGAATTATGAAGACTTTTGCCATATTGCAACAGTATGCACAGAGCAGATCGGCCAGAAGTGCA
AGCGTTTCTTCAGCCCTTCAAATTTTATGAAGTTTGAGAAGGATGATTCTGGGAGAATTGCAAT
CCTACCATTTTATCTTTATGTTATGCGGACAGTAAGTTATTTTCAAACAAATATTATTAGGATT
ACCAAATTTGTTTTGTGTAATATTTGATGTCTTCCATTTGCATCTCTGATAAACTGGTAATTCT
AGCCCACACCATGCTTCTTTTCCAATTTCCCATTGGATGTACTCCTAGTTCTTTTTACTAAATG
ACAAAGTAGGCTAGTTATATGTTATCATCAGTTGCTCCATTATTATTGTTAGTTAGCTTTTATT
AGAAAAGCCATTCAAAGCAATAACACCAAATCGCTATTACAATACAAGAACATGAGTCATGTCA
TTCACCAAAATAAACTTGATGGGTGTAACAATGCCCAAGGAAGCTAAAATCGTTTCCTCCTATT
ACATCAGAATGGGTCACATTTAAGTCTGAAACATCACATAAACTCTGAATTCCCATCCAGCAAA
TTTTTTGCTGAATCCATATTTATAGCTGTAACTTATTTCAAATCATTATCATGCATGCTACTGT
TTCTTTTTCCTTGCGTATACAATTGTATGACATTCTTTTAACTAATTTCTTCAGGTATCTCTCA
CACAAGCGAGAATTGATATGAGTGAGCTTGATGAGGATTCTGATGGTTTCCTTCAACCTCATGT
AAGGACATCCTAACACACGTTAACGCCATTGCACACCTTAGTAATGTTTGACATGACTTAATTC
TATTAACAGGAAATGGAAGCATACATAAGAGGGCTTATCCCCAATTTGGCACAACTGCGTGATA
TGCCATCAGCATTTGTTCAAATGTACTGCCGCATAGCTGCACGCAAGTTCTTTTTCTTCTGCGA
TCCACACAGACGAGGTTTAGAACCAACCTTTAAGTGCAGTTGTATTAATATGCAAATTACTTTA
ATCGATACATTTATTCTTTGTAAGAGTAGCTAACTGTTTGTAATCGATACATTTTTTTTTCAGG
GAAAGCATGCATAAAGAAAGTATTGCTGAGCAATTGTCTTCAGGAACTTATGGAACTACATCAG
GTAATAGAAGAAACATATTTCATGGTTTGCTGGTAGAAACTCTGTAAATTTTATACCCTTATAG
GCTTACAACATAATATTGCTGTATCCTATATTCTGTTTCTTTGTGGTTACGATTCTTATCAACT
ATATTTTCAGGAGAGTGAGGAAGAGGTTACTGATACTGAGCAGGCTGAAAATTGGTTTTCCCTG
ACGTCAGCTCAGCGCATTTGTGGTATGCCAGCCACCCTCATGTCTGGTTTTCTGATTTTTCTTT
TGGGTAGCAGTAGCACACAATCAGTGCTCTCCAAAAAGTTACCTTTTGCCATTCTATTGCAGTA
TAGTTGATGATCTACCTAATGCGCATGGTCCCATGCAGATATGTTTCTTGCACTTGACAAAGAT
ACAAATGGAACATTGAGCAAACAGGAGCTCAAGGAATATGCAGATGGCACATTGACTGATATCT
TCATTGAAAGAG
						
					

			
		

Sequences
Splice junction sequence	ATGTTTCTTGCACTTGACAAAGATACAAATGGAACATTGAGCAAACAGGAGCTCAAGGAATATG CAGATGGCACATTGACTGATATCTTCATTGAAAGAGatgaattatgaagacttttgccatattg caacagtatgcacagagcagatcggccagaagtgcaagcgtttcttcagcccttcaaattttat gaagtttg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 607 Transcript exons: 2848123-2848281,2848817-2848888,2848964-2849096,2849210- 2849274,2849413-2849488,2849633-2849734 ATGAATTATGAAGACTTTTGCCATATTGCAACAGTATGCACAGAGCAGATCGGCCAGAAGTGCA AGCGTTTCTTCAGCCCTTCAAATTTTATGAAGTTTGAGAAGGATGATTCTGGGAGAATTGCAAT CCTACCATTTTATCTTTATGTTATGCGGACAGTATCTCTCACACAAGCGAGAATTGATATGAGT GAGCTTGATGAGGATTCTGATGGTTTCCTTCAACCTCATGAAATGGAAGCATACATAAGAGGGC TTATCCCCAATTTGGCACAACTGCGTGATATGCCATCAGCATTTGTTCAAATGTACTGCCGCAT AGCTGCACGCAAGTTCTTTTTCTTCTGCGATCCACACAGACGAGGGAAAGCATGCATAAAGAAA GTATTGCTGAGCAATTGTCTTCAGGAACTTATGGAACTACATCAGGAGAGTGAGGAAGAGGTTA CTGATACTGAGCAGGCTGAAAATTGGTTTTCCCTGACGTCAGCTCAGCGCATTTGTGATATGTT TCTTGCACTTGACAAAGATACAAATGGAACATTGAGCAAACAGGAGCTCAAGGAATATGCAGAT GGCACATTGACTGATATCTTCATTGAAAGAG
Genomic sequence	ATGAATTATGAAGACTTTTGCCATATTGCAACAGTATGCACAGAGCAGATCGGCCAGAAGTGCA AGCGTTTCTTCAGCCCTTCAAATTTTATGAAGTTTGAGAAGGATGATTCTGGGAGAATTGCAAT CCTACCATTTTATCTTTATGTTATGCGGACAGTAAGTTATTTTCAAACAAATATTATTAGGATT ACCAAATTTGTTTTGTGTAATATTTGATGTCTTCCATTTGCATCTCTGATAAACTGGTAATTCT AGCCCACACCATGCTTCTTTTCCAATTTCCCATTGGATGTACTCCTAGTTCTTTTTACTAAATG ACAAAGTAGGCTAGTTATATGTTATCATCAGTTGCTCCATTATTATTGTTAGTTAGCTTTTATT AGAAAAGCCATTCAAAGCAATAACACCAAATCGCTATTACAATACAAGAACATGAGTCATGTCA TTCACCAAAATAAACTTGATGGGTGTAACAATGCCCAAGGAAGCTAAAATCGTTTCCTCCTATT ACATCAGAATGGGTCACATTTAAGTCTGAAACATCACATAAACTCTGAATTCCCATCCAGCAAA TTTTTTGCTGAATCCATATTTATAGCTGTAACTTATTTCAAATCATTATCATGCATGCTACTGT TTCTTTTTCCTTGCGTATACAATTGTATGACATTCTTTTAACTAATTTCTTCAGGTATCTCTCA CACAAGCGAGAATTGATATGAGTGAGCTTGATGAGGATTCTGATGGTTTCCTTCAACCTCATGT AAGGACATCCTAACACACGTTAACGCCATTGCACACCTTAGTAATGTTTGACATGACTTAATTC TATTAACAGGAAATGGAAGCATACATAAGAGGGCTTATCCCCAATTTGGCACAACTGCGTGATA TGCCATCAGCATTTGTTCAAATGTACTGCCGCATAGCTGCACGCAAGTTCTTTTTCTTCTGCGA TCCACACAGACGAGGTTTAGAACCAACCTTTAAGTGCAGTTGTATTAATATGCAAATTACTTTA ATCGATACATTTATTCTTTGTAAGAGTAGCTAACTGTTTGTAATCGATACATTTTTTTTTCAGG GAAAGCATGCATAAAGAAAGTATTGCTGAGCAATTGTCTTCAGGAACTTATGGAACTACATCAG GTAATAGAAGAAACATATTTCATGGTTTGCTGGTAGAAACTCTGTAAATTTTATACCCTTATAG GCTTACAACATAATATTGCTGTATCCTATATTCTGTTTCTTTGTGGTTACGATTCTTATCAACT ATATTTTCAGGAGAGTGAGGAAGAGGTTACTGATACTGAGCAGGCTGAAAATTGGTTTTCCCTG ACGTCAGCTCAGCGCATTTGTGGTATGCCAGCCACCCTCATGTCTGGTTTTCTGATTTTTCTTT TGGGTAGCAGTAGCACACAATCAGTGCTCTCCAAAAAGTTACCTTTTGCCATTCTATTGCAGTA TAGTTGATGATCTACCTAATGCGCATGGTCCCATGCAGATATGTTTCTTGCACTTGACAAAGAT ACAAATGGAACATTGAGCAAACAGGAGCTCAAGGAATATGCAGATGGCACATTGACTGATATCT TCATTGAAAGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_015868 						
					
						PMCS
						0.286389283

Conservation Information
Conserved circRNAs	osi_circ_015868
PMCS	0.286389283

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2848124-2848130(+)
						
						Potential amino acid sequence
						
							MNYEDFCHIATVCTEQIGQKCKRFFSPSNFMKFEKDDSGRIAILPFYLYVMRTVSLTQARIDMS
ELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFVQMYCRIAARKFFFFCDPHRRGKACIKK
VLLSNCLQELMELHQESEEEVTDTEQAENWFSLTSAQRICDMFLALDKDTNGTLSKQELKEYAD
GTLTDIFIERDEL*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2848124-2848130(+)
Potential amino acid sequence	MNYEDFCHIATVCTEQIGQKCKRFFSPSNFMKFEKDDSGRIAILPFYLYVMRTVSLTQARIDMS ELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFVQMYCRIAARKFFFFCDPHRRGKACIKK VLLSNCLQELMELHQESEEEVTDTEQAENWFSLTSAQRICDMFLALDKDTNGTLSKQELKEYAD GTLTDIFIERDEL*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os05g0149800_circ_g.3

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