Plant Circular RNA Database

Detail information of AT5G14740_circ_g.13

			
						 General Information
					
						CircRNA Name
						AT5G14740_circ_g.13
					
						ID in PlantcircBase
						ath_circ_038298
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 4761163-4761480  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							AT5G14740						
					
						Parent gene annotation
						
							Beta carbonic anhydrase 2, chloroplastic
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G14740_circ_g.1 AT5G14740_circ_g.2 AT5G14740_circ_g.3 AT5G14740_circ_g.4 AT5G14740_circ_g.5 AT5G14740_circ_g.6 AT5G14740_circ_g.7 AT5G14740_circ_g.8 AT5G14740_circ_g.9 AT5G14740_circ_g.10 AT5G14740_circ_g.11 AT5G14740_circ_g.12 AT5G14740_circ_g.14 AT5G14740_circ_g.15 AT5G14740_circ_g.16 AT5G14740_circ_g.17 AT5G14740_circ_g.18 AT5G14740_circ_g.19 AT5G14740_circ_g.20 AT5G14740_circ_g.21 AT5G14740_circ_g.22 						
					
						Support reads
						71
					
						Tissues
						aerial
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G14740.8:2
AT5G14740.6:2
AT5G14740.3:2
AT5G14740.4:2
AT5G14740.1:2
AT5G14740.2:2
AT5G14740.7:2
AT5G14740.5:2
AT5G14740.9:2

General Information
CircRNA Name	AT5G14740_circ_g.13
ID in PlantcircBase	ath_circ_038298
Alias	NA
Organism	Arabidpsis thaliana
Position	chr5: 4761163-4761480 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	AT5G14740
Parent gene annotation	Beta carbonic anhydrase 2, chloroplastic
Parent gene strand	+
Alternative splicing	AT5G14740_circ_g.1 AT5G14740_circ_g.2 AT5G14740_circ_g.3 AT5G14740_circ_g.4 AT5G14740_circ_g.5 AT5G14740_circ_g.6 AT5G14740_circ_g.7 AT5G14740_circ_g.8 AT5G14740_circ_g.9 AT5G14740_circ_g.10 AT5G14740_circ_g.11 AT5G14740_circ_g.12 AT5G14740_circ_g.14 AT5G14740_circ_g.15 AT5G14740_circ_g.16 AT5G14740_circ_g.17 AT5G14740_circ_g.18 AT5G14740_circ_g.19 AT5G14740_circ_g.20 AT5G14740_circ_g.21 AT5G14740_circ_g.22
Support reads	71
Tissues	aerial
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G14740.8:2 AT5G14740.6:2 AT5G14740.3:2 AT5G14740.4:2 AT5G14740.1:2 AT5G14740.2:2 AT5G14740.7:2 AT5G14740.5:2 AT5G14740.9:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AACATGGCTTTTCTGTTTTTTTTTTCCTTTATAAAAATAATTACAGGTCAAATATGCAGGAGTT
GGAGCCGCCATTGAATACGCTGTCTTGCACCTTAAGtacatggtgtttgcttgttcggactcac
gagtgtgcccatcacacgtactagacttccatcctggagatgccttcgtggttcgtaatatcgc
caatatgg

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TACATGGTGTTTGCTTGTTCGGACTCACGAGTGTGCCCATCACACGTACTAGACTTCCATCCTG
GAGATGCCTTCGTGGTTCGTAATATCGCCAATATGGTTCCTCCTTTTGACAAGGTAAACACTTT
ATACACTACATTAATTTACATCGCTAATTTATTCTTGAACATTCTGACTCAAACAGAGTGTTTG
ACTTTGATTTTGACTTATGAAGTCATAACATGGCTTTTCTGTTTTTTTTTTCCTTTATAAAAAT
AATTACAGGTCAAATATGCAGGAGTTGGAGCCGCCATTGAATACGCTGTCTTGCACCTTAAG

Sequences
Splice junction sequence	AACATGGCTTTTCTGTTTTTTTTTTCCTTTATAAAAATAATTACAGGTCAAATATGCAGGAGTT GGAGCCGCCATTGAATACGCTGTCTTGCACCTTAAGtacatggtgtttgcttgttcggactcac gagtgtgcccatcacacgtactagacttccatcctggagatgccttcgtggttcgtaatatcgc caatatgg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TACATGGTGTTTGCTTGTTCGGACTCACGAGTGTGCCCATCACACGTACTAGACTTCCATCCTG GAGATGCCTTCGTGGTTCGTAATATCGCCAATATGGTTCCTCCTTTTGACAAGGTAAACACTTT ATACACTACATTAATTTACATCGCTAATTTATTCTTGAACATTCTGACTCAAACAGAGTGTTTG ACTTTGATTTTGACTTATGAAGTCATAACATGGCTTTTCTGTTTTTTTTTTCCTTTATAAAAAT AATTACAGGTCAAATATGCAGGAGTTGGAGCCGCCATTGAATACGCTGTCTTGCACCTTAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.177479272

Conservation Information
Conserved circRNAs	NA
PMCS	0.177479272

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4761166-4761477(+)
4761456-4761165(-)
						
						Potential amino acid sequence
						
							MVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKYMVFACSD
SRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKYMVFACSDSRVCPSH
VLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKYMVFACSDSRVCPSHVLDFHPG
DAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLK(+)
MAAPTPAYLTLSKGGTILAILRTTKASPGWKSSTCDGHTRESEQANTMYLRCKTAYSMAAPTPA
YLTLSKGGTILAILRTTKASPGWKSSTCDGHTRESEQANTMYLRCKTAYSMAAPTPAYLTLSKG
GTILAILRTTKASPGWKSSTCDGHTRESEQANTMYLRCKTAYSMAAPTPAYLTLSKGGTILAIL
RTTKASPGWKSSTCDGHTRESEQANTM(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	4761166-4761477(+) 4761456-4761165(-)
Potential amino acid sequence	MVFACSDSRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKYMVFACSD SRVCPSHVLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKYMVFACSDSRVCPSH VLDFHPGDAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLKYMVFACSDSRVCPSHVLDFHPG DAFVVRNIANMVPPFDKVKYAGVGAAIEYAVLHLK(+) MAAPTPAYLTLSKGGTILAILRTTKASPGWKSSTCDGHTRESEQANTMYLRCKTAYSMAAPTPA YLTLSKGGTILAILRTTKASPGWKSSTCDGHTRESEQANTMYLRCKTAYSMAAPTPAYLTLSKG GTILAILRTTKASPGWKSSTCDGHTRESEQANTMYLRCKTAYSMAAPTPAYLTLSKGGTILAIL RTTKASPGWKSSTCDGHTRESEQANTM(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT5G14740_circ_g.13

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