Plant Circular RNA Database

Detail information of Os05g0215800_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os05g0215800_circ_g.1
					
						ID in PlantcircBase
						osa_circ_027006
					
						Alias
						Os05circ05192/Os_ciR10268
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 7173183-7174565  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl, find_circ
					
						Parent gene
						
							Os05g0215800						
					
						Parent gene annotation
						
							Protein of unknown function DUF250 domain containing protein. (O
s05t0215800-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						4/2/1
					
						Tissues
						leaf and panicle/root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0215800-01:4

General Information
CircRNA Name	Os05g0215800_circ_g.1
ID in PlantcircBase	osa_circ_027006
Alias	Os05circ05192/Os_ciR10268
Organism	Oryza sativa
Position	chr5: 7173183-7174565 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl, find_circ
Parent gene	Os05g0215800
Parent gene annotation	Protein of unknown function DUF250 domain containing protein. (O s05t0215800-01)
Parent gene strand	-
Alternative splicing	NA
Support reads	4/2/1
Tissues	leaf and panicle/root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0215800-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCCAGCATCTAATGCTGCTTTTCTCTCTGCCTTGCTTCCAGGTGCCATGTTCTCTTGTCATGTA
GAATCTAATCCAATTTGGATTTTGGAGTATCACAATcgtaactattgctgtgtaattgaaagta
tcaactcttttgttggtcagccagaagtccacgaaaggccccagtactagtaaagatgctgcct
gagctgga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 730
Transcript exons: 7173183-7173344,7173791-7173991,7174073-7174257,7174384-
7174565
CGTAACTATTGCTGTGTAATTGAAAGTATCAACTCTTTTGTTGGTCAGCCAGAAGTCCACGAAA
GGCCCCAGTACTAGTAAAGATGCTGCCTGAGCTGGAGCAGTATGACCCAAGAGGTTGAATGAGC
CAAGCGAGTACTTCCTTTGAAGGTGATGAACATAATGCTGTTGTAGTGCAGTGCTCCAAACCGC
TATTATGGCAGCTATGAGCCCTTGTGAATTCACACTAACATCAGTCACAGTACACACGGCAACA
CCTACTAGAACAACCATTATACTGAACTTTGTATCTCTTGAGTATCGGACTTTATCAAACAGGA
TTTCCAGAAAGCACAAAACTGGAATGATGCATAACTTGGCAATCTGGTAGAATCCAACAGAGTT
CCACATCAAACTAACATTCATCCCAACAATTGATAGGTTTGCAAAAAAGACAAATTTTATTAGT
TCTGGCACTGGTAAGTAGGACGGTTGAATATATCCCAGCCATTTCATTACTAATGTCATCAAAG
TCGTGGTTGCAAAATGCAGTCCAGTCAATGTTGTAGCAAAACTAAAACCATGTGTAGCCATTAA
GGCCTTGTTGACCATGATGATACCAACAGATGTCACAATGTTGAACATCCAAGCTCCAGCATCT
AATGCTGCTTTTCTCTCTGCCTTGCTTCCAGGTGCCATGTTCTCTTGTCATGTAGAATCTAATC
CAATTTGGATTTTGGAGTATCACAAT
						
					

						Genomic sequence
						
							CGTAACTATTGCTGTGTAATTGAAAGTATCAACTCTTTTGTTGGTCAGCCAGAAGTCCACGAAA
GGCCCCAGTACTAGTAAAGATGCTGCCTGAGCTGGAGCAGTATGACCCAAGAGGTTGAATGAGC
CAAGCGAGTACTTCCTTTGAAGGTGATGAACATACTGCAGCACAGAAGCAAAGAAACAAAACAA
GTAATAGTGTTAAGAAAATTTGCCATGAAAGTGCGTAGAAAAGGGACAAGACTTCTATTAGCAG
ACAAACGAGCAATATCCAAATATTTGAAGATCTTTTACTAACTAAACATTTCTTTTCTACACGA
TTAATCAGAACAGTAGTTAGATGGAAGCTAATACATTCTTTAGCTCACCAAACCTACAACAAAG
GGGACAAAATTCAGGTTTATAGGATGAGGCAAAATACACATGAGTTCACATTAATGGCTTGGAA
ATTCTGTAGAAGGGAACACCACATACTATTTTTTTTCAGGTTTGAATATTACCAACTTGGGAGC
AAAAGTATCAAAGTAGAGACTTAGTTAATACAGGAGAATTCTCAGTATGTAGGAATGGGGACAA
CAAAGGCACAATCTAGGAGTGGCAATACTCACATGCTGTTGTAGTGCAGTGCTCCAAACCGCTA
TTATGGCAGCTATGAGCCCTTGTGAATTCACACTAACATCAGTCACAGTACACACGGCAACACC
TACTAGAACAACCATTATACTGAACTTTGTATCTCTTGAGTATCGGACTTTATCAAACAGGATT
TCCAGAAAGCACAAAACTGGAATGATGCATAACTTGGCAATCTGAAAAAGGCATGAGGAGCAAA
TTAGTATATGCATCCAATTCTAAAAGTGAACATTAAAGGACTTAGTTACTTTCTTCACCTGGTA
GAATCCAACAGAGTTCCACATCAAACTAACATTCATCCCAACAATTGATAGGTTTGCAAAAAAG
ACAAATTTTATTAGTTCTGGCACTGGTAAGTAGGACGGTTGAATATATCCCAGCCATTTCATTA
CTAATGTCATCAAAGTCGTGGTTGCAAAATGCAGTCCAGTCAATGTTGTAGCTGTACAAGCAAT
AGCACCCCCATATTAGTACAAGCATGTGTTCAGTAGCTGAGTTTGTTACAGATTCATCTTTCCA
AGTGATCACACATATGAGTAAATAATAAAGCATTATCAATTTATCGTACCAAAACTAAAACCAT
GTGTAGCCATTAAGGCCTTGTTGACCATGATGATACCAACAGATGTCACAATGTTGAACATCCA
AGCTCCAGCATCTAATGCTGCTTTTCTCTCTGCCTTGCTTCCAGGTGCCATGTTCTCTTGTCAT
GTAGAATCTAATCCAATTTGGATTTTGGAGTATCACAAT
						
					

			
		

Sequences
Splice junction sequence	TCCAGCATCTAATGCTGCTTTTCTCTCTGCCTTGCTTCCAGGTGCCATGTTCTCTTGTCATGTA GAATCTAATCCAATTTGGATTTTGGAGTATCACAATcgtaactattgctgtgtaattgaaagta tcaactcttttgttggtcagccagaagtccacgaaaggccccagtactagtaaagatgctgcct gagctgga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 730 Transcript exons: 7173183-7173344,7173791-7173991,7174073-7174257,7174384- 7174565 CGTAACTATTGCTGTGTAATTGAAAGTATCAACTCTTTTGTTGGTCAGCCAGAAGTCCACGAAA GGCCCCAGTACTAGTAAAGATGCTGCCTGAGCTGGAGCAGTATGACCCAAGAGGTTGAATGAGC CAAGCGAGTACTTCCTTTGAAGGTGATGAACATAATGCTGTTGTAGTGCAGTGCTCCAAACCGC TATTATGGCAGCTATGAGCCCTTGTGAATTCACACTAACATCAGTCACAGTACACACGGCAACA CCTACTAGAACAACCATTATACTGAACTTTGTATCTCTTGAGTATCGGACTTTATCAAACAGGA TTTCCAGAAAGCACAAAACTGGAATGATGCATAACTTGGCAATCTGGTAGAATCCAACAGAGTT CCACATCAAACTAACATTCATCCCAACAATTGATAGGTTTGCAAAAAAGACAAATTTTATTAGT TCTGGCACTGGTAAGTAGGACGGTTGAATATATCCCAGCCATTTCATTACTAATGTCATCAAAG TCGTGGTTGCAAAATGCAGTCCAGTCAATGTTGTAGCAAAACTAAAACCATGTGTAGCCATTAA GGCCTTGTTGACCATGATGATACCAACAGATGTCACAATGTTGAACATCCAAGCTCCAGCATCT AATGCTGCTTTTCTCTCTGCCTTGCTTCCAGGTGCCATGTTCTCTTGTCATGTAGAATCTAATC CAATTTGGATTTTGGAGTATCACAAT
Genomic sequence	CGTAACTATTGCTGTGTAATTGAAAGTATCAACTCTTTTGTTGGTCAGCCAGAAGTCCACGAAA GGCCCCAGTACTAGTAAAGATGCTGCCTGAGCTGGAGCAGTATGACCCAAGAGGTTGAATGAGC CAAGCGAGTACTTCCTTTGAAGGTGATGAACATACTGCAGCACAGAAGCAAAGAAACAAAACAA GTAATAGTGTTAAGAAAATTTGCCATGAAAGTGCGTAGAAAAGGGACAAGACTTCTATTAGCAG ACAAACGAGCAATATCCAAATATTTGAAGATCTTTTACTAACTAAACATTTCTTTTCTACACGA TTAATCAGAACAGTAGTTAGATGGAAGCTAATACATTCTTTAGCTCACCAAACCTACAACAAAG GGGACAAAATTCAGGTTTATAGGATGAGGCAAAATACACATGAGTTCACATTAATGGCTTGGAA ATTCTGTAGAAGGGAACACCACATACTATTTTTTTTCAGGTTTGAATATTACCAACTTGGGAGC AAAAGTATCAAAGTAGAGACTTAGTTAATACAGGAGAATTCTCAGTATGTAGGAATGGGGACAA CAAAGGCACAATCTAGGAGTGGCAATACTCACATGCTGTTGTAGTGCAGTGCTCCAAACCGCTA TTATGGCAGCTATGAGCCCTTGTGAATTCACACTAACATCAGTCACAGTACACACGGCAACACC TACTAGAACAACCATTATACTGAACTTTGTATCTCTTGAGTATCGGACTTTATCAAACAGGATT TCCAGAAAGCACAAAACTGGAATGATGCATAACTTGGCAATCTGAAAAAGGCATGAGGAGCAAA TTAGTATATGCATCCAATTCTAAAAGTGAACATTAAAGGACTTAGTTACTTTCTTCACCTGGTA GAATCCAACAGAGTTCCACATCAAACTAACATTCATCCCAACAATTGATAGGTTTGCAAAAAAG ACAAATTTTATTAGTTCTGGCACTGGTAAGTAGGACGGTTGAATATATCCCAGCCATTTCATTA CTAATGTCATCAAAGTCGTGGTTGCAAAATGCAGTCCAGTCAATGTTGTAGCTGTACAAGCAAT AGCACCCCCATATTAGTACAAGCATGTGTTCAGTAGCTGAGTTTGTTACAGATTCATCTTTCCA AGTGATCACACATATGAGTAAATAATAAAGCATTATCAATTTATCGTACCAAAACTAAAACCAT GTGTAGCCATTAAGGCCTTGTTGACCATGATGATACCAACAGATGTCACAATGTTGAACATCCA AGCTCCAGCATCTAATGCTGCTTTTCTCTCTGCCTTGCTTCCAGGTGCCATGTTCTCTTGTCAT GTAGAATCTAATCCAATTTGGATTTTGGAGTATCACAAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_016011 						
					
						PMCS
						0.405617366

Conservation Information
Conserved circRNAs	osi_circ_016011
PMCS	0.405617366

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7174426-7173257(+)
7174515-7174391(-)
						
						Potential amino acid sequence
						
							MMIPTDVTMLNIQAPASNAAFLSALLPGAMFSCHVESNPIWILEYHNRNYCCVIESINSFVGQP
EVHERPQY*(+)
MAPGSKAERKAALDAGAWMFNIVTSVGIIMVNKALMATHGFSFATTLTGLHFATTTLMTLVMKW
LGYIQPSYLPVPELIKFVFFANLSIVGMNVSLMWNSVGFYQIAKLCIIPVLCFLEILFDKVRYS
RDTKFSIMVVLVGVAVCTVTDVSVNSQGLIAAIIAVWSTALQQHYVHHLQRKYSLGSFNLLGHT
APAQAASLLVLGPFVDFWLTNKRVDTFNYTAIVTIVILQNPNWIRFYMTREHGTWKQGREKSSI
RCWSLDVQHCDICWYHHGQQGLNGYTWF*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	7174426-7173257(+) 7174515-7174391(-)
Potential amino acid sequence	MMIPTDVTMLNIQAPASNAAFLSALLPGAMFSCHVESNPIWILEYHNRNYCCVIESINSFVGQP EVHERPQY(+) MAPGSKAERKAALDAGAWMFNIVTSVGIIMVNKALMATHGFSFATTLTGLHFATTTLMTLVMKW LGYIQPSYLPVPELIKFVFFANLSIVGMNVSLMWNSVGFYQIAKLCIIPVLCFLEILFDKVRYS RDTKFSIMVVLVGVAVCTVTDVSVNSQGLIAAIIAVWSTALQQHYVHHLQRKYSLGSFNLLGHT APAQAASLLVLGPFVDFWLTNKRVDTFNYTAIVTIVILQNPNWIRFYMTREHGTWKQGREKSSI RCWSLDVQHCDICWYHHGQQGLNGYTWF(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os05g0215800_circ_g.1

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