Plant Circular RNA Database

Detail information of Os04g0659100_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os04g0659100_circ_g.2
					
						ID in PlantcircBase
						osa_circ_025772
					
						Alias
						Os04circ16432
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr4: 33625263-33625563  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os04g0659100						
					
						Parent gene annotation
						
							Glutamine synthetase shoot isozyme, chloroplast precursor (EC 6.
3.1.2) (Glutamate--ammonia ligase) (Clone lambda-GS31). (Os04t06
59100-01);Similar to Isoform 2 of Glutamine synthetase, chloropl
astic. (Os04t0659100-02);Glutamine synthetase shoot isozyme, chl
oroplast precursor (EC 6.3.1.2) (Glutamate--ammonia ligase) (Clo
ne lambda-GS31). (Os04t0659100-03)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os04g0659100_circ_igg.1 Os04g0659100_circ_g.2 Os04g0659100_circ_g.3 Os04g0659100_circ_g.4 Os04g0659100_circ_g.5 Os04g0659100_circ_g.6 Os04g0659100_circ_g.7 Os04g0659100_circ_g.8 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os04t0659100-03:2
Os04t0659100-01:2

General Information
CircRNA Name	Os04g0659100_circ_g.2
ID in PlantcircBase	osa_circ_025772
Alias	Os04circ16432
Organism	Oryza sativa
Position	chr4: 33625263-33625563 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os04g0659100
Parent gene annotation	Glutamine synthetase shoot isozyme, chloroplast precursor (EC 6. 3.1.2) (Glutamate--ammonia ligase) (Clone lambda-GS31). (Os04t06 59100-01);Similar to Isoform 2 of Glutamine synthetase, chloropl astic. (Os04t0659100-02);Glutamine synthetase shoot isozyme, chl oroplast precursor (EC 6.3.1.2) (Glutamate--ammonia ligase) (Clo ne lambda-GS31). (Os04t0659100-03)
Parent gene strand	-
Alternative splicing	Os04g0659100_circ_igg.1 Os04g0659100_circ_g.2 Os04g0659100_circ_g.3 Os04g0659100_circ_g.4 Os04g0659100_circ_g.5 Os04g0659100_circ_g.6 Os04g0659100_circ_g.7 Os04g0659100_circ_g.8
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os04t0659100-03:2 Os04t0659100-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTCACCATATGCACTTATATGCAAGTCATGGCGAAGTGATAGGTTTAGGATTGCCTTCTTGATC
ACCTCAAATCCTCCATCTTCACGCATACTCTTGGTGcttttcccttcgcctcggtgtctcgccc
cacccgaatagagcatccacggtttgccacaccctttaatgaaatgtttcgttagtatagctta
aaaataga

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CTTTTCCCTTCGCCTCGGTGTCTCGCCCCACCCGAATAGAGCATCCACGGTTTGCCACACCCTT
TAATGAAATGTTTCGTTAGTATAGCTTAAAAATAGAAATTAAAACATAAAGATATAAAAAGGCC
GCTGAAACCATACCCATGAGAAATTGTCAATGCTAGCTGTCTCGTGTAAACCTGTCAACCTCCT
TTCATTTCCTTCACCATATGCACTTATATGCAAGTCATGGCGAAGTGATAGGTTTAGGATTGCC
TTCTTGATCACCTCAAATCCTCCATCTTCACGCATACTCTTGGTG

Sequences
Splice junction sequence	TTCACCATATGCACTTATATGCAAGTCATGGCGAAGTGATAGGTTTAGGATTGCCTTCTTGATC ACCTCAAATCCTCCATCTTCACGCATACTCTTGGTGcttttcccttcgcctcggtgtctcgccc cacccgaatagagcatccacggtttgccacaccctttaatgaaatgtttcgttagtatagctta aaaataga
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTTTTCCCTTCGCCTCGGTGTCTCGCCCCACCCGAATAGAGCATCCACGGTTTGCCACACCCTT TAATGAAATGTTTCGTTAGTATAGCTTAAAAATAGAAATTAAAACATAAAGATATAAAAAGGCC GCTGAAACCATACCCATGAGAAATTGTCAATGCTAGCTGTCTCGTGTAAACCTGTCAACCTCCT TTCATTTCCTTCACCATATGCACTTATATGCAAGTCATGGCGAAGTGATAGGTTTAGGATTGCC TTCTTGATCACCTCAAATCCTCCATCTTCACGCATACTCTTGGTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.255120017

Conservation Information
Conserved circRNAs	NA
PMCS	0.255120017

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							33625421-33625298(+)
33625409-33625528(-)
33625555-33625528(-)
						
						Potential amino acid sequence
						
							MLAVSCKPVNLLSFPSPYALICKSWRSDRFRIAFLITSNPPSSRILLVLFPSPRCLAPPE*(+)
MGCGKPWMLYSGGARHRGEGKSTKSMREDGGFEVIKKAILNLSLRHDLHISAYGEGNERRLTGL
HETASIDNFSWGVANRGCSIRVGRDTEAKGKAPRVCVKMEDLR*(-)
MREDGGFEVIKKAILNLSLRHDLHISAYGEGNERRLTGLHETASIDNFSWGVANRGCSIRVGRD
TEAKGKAPRVCVKMEDLR*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	33625421-33625298(+) 33625409-33625528(-) 33625555-33625528(-)
Potential amino acid sequence	MLAVSCKPVNLLSFPSPYALICKSWRSDRFRIAFLITSNPPSSRILLVLFPSPRCLAPPE(+) MGCGKPWMLYSGGARHRGEGKSTKSMREDGGFEVIKKAILNLSLRHDLHISAYGEGNERRLTGL HETASIDNFSWGVANRGCSIRVGRDTEAKGKAPRVCVKMEDLR(-) MREDGGFEVIKKAILNLSLRHDLHISAYGEGNERRLTGLHETASIDNFSWGVANRGCSIRVGRD TEAKGKAPRVCVKMEDLR*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os04g0659100_circ_g.2

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