Plant Circular RNA Database

Detail information of AT2G43500_circ_g.1

			
						 General Information
					
						CircRNA Name
						AT2G43500_circ_g.1
					
						ID in PlantcircBase
						ath_circ_018170
					
						Alias
						AT2G43500_C1, AT2G43500_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 18062531-18063659  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							AT2G43500						
					
						Parent gene annotation
						
							Plant regulator RWP-RK family protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G43500_circ_g.2 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G43500.9:2
AT2G43500.3:1
AT2G43500.6:2
AT2G43500.1:2
AT2G43500.7:2
AT2G43500.2:2
AT2G43500.10:1
AT2G43500.11:2
AT2G43500.8:2
AT2G43500.5:2
AT2G43500.4:2

General Information
CircRNA Name	AT2G43500_circ_g.1
ID in PlantcircBase	ath_circ_018170
Alias	AT2G43500_C1, AT2G43500_C1
Organism	Arabidpsis thaliana
Position	chr2: 18062531-18063659 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	CIRI2
Parent gene	AT2G43500
Parent gene annotation	Plant regulator RWP-RK family protein
Parent gene strand	+
Alternative splicing	AT2G43500_circ_g.2
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G43500.9:2 AT2G43500.3:1 AT2G43500.6:2 AT2G43500.1:2 AT2G43500.7:2 AT2G43500.2:2 AT2G43500.10:1 AT2G43500.11:2 AT2G43500.8:2 AT2G43500.5:2 AT2G43500.4:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGCTTCTGGGACATCTTGTTGTGCTGTCATGGAACTCGTTACATCCAAGGAAAAACCTAATTTT
GATATGGAGATGGACTCTGTTTGCCGTGCTCTCCAGgttactggtggaggtttgattcattaaa
ttttgctacgttgtgggatcttcaagaagaatcctttgtatgtaagattccgtttgaagattct
gtctttta

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTTACTGGTGGAGGTTTGATTCATTAAATTTTGCTACGTTGTGGGATCTTCAAGAAGAATCCTT
TGTATGTAAGATTCCGTTTGAAGATTCTGTCTTTTAATTTGTTAGTAATGTTTAGTTTGTTATT
CAACTTGATGTGTCTTATTTTTCATGCTCTAGCAGTTTAAATCTGTCATCGAAGATATGGAAAA
CCCTTTTGCTTCTAGAGAAAAGGGGTTTGGTAATTATTCAGATTTTCCAACAGAACAAATGGAT
GGTTTAAGCTCAAACTTTGGTTCTGGCGTTAGGAATTTGATCTCTGATGATATGTTCAACCCTT
CCTCCGAGCTTATGAATTTCGACTCTTTGGCCGCATGGTGTAACAGCCCTTCCGCTACTGATAT
ATTGTTCGCCCAATATGGTCTGTCGAACTCCCAACCTATGCCTTTTGGAGCTTTCACTTCATTT
CATGTAGCTGACCCGAAGGCTACCAGTCTCACTCGTTCGTTCTATGATTTGGAAAGCTCTTACT
ATGGTGAAGAAAGATCGTCAGCACAGGAAATGAATTCTCAGTTTCATCGCTCTTCAGATAGCGA
TGAGTTAAGTGGTAAACGACGCAAGGTTGTTAATCAGAAGATTGGTTTTCCTAACGTACTCAAC
TGTACTATTCCCAGGTCTTTGAGCCACTCATTAGATGAGAAGATGCTTAAGGCATTAAGCTTAT
TTATGGAGTCCTCAGGTTCAGGTGAGGGCATTTTAGCGCAAGTTTGGACCCCTATCAAGACAGG
AGATCAGTACTTGCTCAGTACTTGTGATCAGGCCTATTTGCTTGATCCGAGGTTTTCTCAGTAC
CGTGAAGTGTCAAGGAGATTTACGTTTGCTGCTGAAGCAAATCAATGTTCTTTTCCGGGTCTTC
CTGGCCGAGTCTTTATCTCGGGAGTACCTGAATGGACATCAAACGTTATGTATTACAAGACCGA
TGAGTATTTGCGCATGAAGCATGCAATTGACAATGAAGTCCGTGGTTCCATTGCAATACCTATC
CTTGAAGCTTCTGGGACATCTTGTTGTGCTGTCATGGAACTCGTTACATCCAAGGAAAAACCTA
ATTTTGATATGGAGATGGACTCTGTTTGCCGTGCTCTCCAG

Sequences
Splice junction sequence	AGCTTCTGGGACATCTTGTTGTGCTGTCATGGAACTCGTTACATCCAAGGAAAAACCTAATTTT GATATGGAGATGGACTCTGTTTGCCGTGCTCTCCAGgttactggtggaggtttgattcattaaa ttttgctacgttgtgggatcttcaagaagaatcctttgtatgtaagattccgtttgaagattct gtctttta
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTTACTGGTGGAGGTTTGATTCATTAAATTTTGCTACGTTGTGGGATCTTCAAGAAGAATCCTT TGTATGTAAGATTCCGTTTGAAGATTCTGTCTTTTAATTTGTTAGTAATGTTTAGTTTGTTATT CAACTTGATGTGTCTTATTTTTCATGCTCTAGCAGTTTAAATCTGTCATCGAAGATATGGAAAA CCCTTTTGCTTCTAGAGAAAAGGGGTTTGGTAATTATTCAGATTTTCCAACAGAACAAATGGAT GGTTTAAGCTCAAACTTTGGTTCTGGCGTTAGGAATTTGATCTCTGATGATATGTTCAACCCTT CCTCCGAGCTTATGAATTTCGACTCTTTGGCCGCATGGTGTAACAGCCCTTCCGCTACTGATAT ATTGTTCGCCCAATATGGTCTGTCGAACTCCCAACCTATGCCTTTTGGAGCTTTCACTTCATTT CATGTAGCTGACCCGAAGGCTACCAGTCTCACTCGTTCGTTCTATGATTTGGAAAGCTCTTACT ATGGTGAAGAAAGATCGTCAGCACAGGAAATGAATTCTCAGTTTCATCGCTCTTCAGATAGCGA TGAGTTAAGTGGTAAACGACGCAAGGTTGTTAATCAGAAGATTGGTTTTCCTAACGTACTCAAC TGTACTATTCCCAGGTCTTTGAGCCACTCATTAGATGAGAAGATGCTTAAGGCATTAAGCTTAT TTATGGAGTCCTCAGGTTCAGGTGAGGGCATTTTAGCGCAAGTTTGGACCCCTATCAAGACAGG AGATCAGTACTTGCTCAGTACTTGTGATCAGGCCTATTTGCTTGATCCGAGGTTTTCTCAGTAC CGTGAAGTGTCAAGGAGATTTACGTTTGCTGCTGAAGCAAATCAATGTTCTTTTCCGGGTCTTC CTGGCCGAGTCTTTATCTCGGGAGTACCTGAATGGACATCAAACGTTATGTATTACAAGACCGA TGAGTATTTGCGCATGAAGCATGCAATTGACAATGAAGTCCGTGGTTCCATTGCAATACCTATC CTTGAAGCTTCTGGGACATCTTGTTGTGCTGTCATGGAACTCGTTACATCCAAGGAAAAACCTA ATTTTGATATGGAGATGGACTCTGTTTGCCGTGCTCTCCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.160422874

Conservation Information
Conserved circRNAs	NA
PMCS	0.160422874

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							18062716-18062554(+)
18063512-18062546(+)
						
						Potential amino acid sequence
						
							MENPFASREKGFGNYSDFPTEQMDGLSSNFGSGVRNLISDDMFNPSSELMNFDSLAAWCNSPSA
TDILFAQYGLSNSQPMPFGAFTSFHVADPKATSLTRSFYDLESSYYGEERSSAQEMNSQFHRSS
DSDELSGKRRKVVNQKIGFPNVLNCTIPRSLSHSLDEKMLKALSLFMESSGSGEGILAQVWTPI
KTGDQYLLSTCDQAYLLDPRFSQYREVSRRFTFAAEANQCSFPGLPGRVFISGVPEWTSNVMYY
KTDEYLRMKHAIDNEVRGSIAIPILEASGTSCCAVMELVTSKEKPNFDMEMDSVCRALQVTGGG
LIH*
MQLTMKSVVPLQYLSLKLLGHLVVLSWNSLHPRKNLILIWRWTLFAVLSRLLVEV*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	18062716-18062554(+) 18063512-18062546(+)
Potential amino acid sequence	MENPFASREKGFGNYSDFPTEQMDGLSSNFGSGVRNLISDDMFNPSSELMNFDSLAAWCNSPSA TDILFAQYGLSNSQPMPFGAFTSFHVADPKATSLTRSFYDLESSYYGEERSSAQEMNSQFHRSS DSDELSGKRRKVVNQKIGFPNVLNCTIPRSLSHSLDEKMLKALSLFMESSGSGEGILAQVWTPI KTGDQYLLSTCDQAYLLDPRFSQYREVSRRFTFAAEANQCSFPGLPGRVFISGVPEWTSNVMYY KTDEYLRMKHAIDNEVRGSIAIPILEASGTSCCAVMELVTSKEKPNFDMEMDSVCRALQVTGGG LIH* MQLTMKSVVPLQYLSLKLLGHLVVLSWNSLHPRKNLILIWRWTLFAVLSRLLVEV*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Zhang et al., 2019

circRNA

Detail information of AT2G43500_circ_g.1

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