Plant Circular RNA Database

Detail information of Os01g0254100_circ_g.16

			
						 General Information
					
						CircRNA Name
						Os01g0254100_circ_g.16
					
						ID in PlantcircBase
						osa_circ_001149
					
						Alias
						Os_ciR2776
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 8428423-8428848  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os01g0254100						
					
						Parent gene annotation
						
							Similar to CTV.2. (Os01t0254100-01);Similar to Lissencephaly typ
e-1-like homology motif; CTLH, C-terminal to LisH motif; Nitrous
 oxide reductase, N-terminal; WD40-like; Quinonprotein alcohol d
ehydrogenase-like. (Os01t0254100-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os01g0254100_circ_g.17 Os01g0254100_circ_g.18 						
					
						Support reads
						4/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0254100-01:2
Os01t0254100-02:2

General Information
CircRNA Name	Os01g0254100_circ_g.16
ID in PlantcircBase	osa_circ_001149
Alias	Os_ciR2776
Organism	Oryza sativa
Position	chr1: 8428423-8428848 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os01g0254100
Parent gene annotation	Similar to CTV.2. (Os01t0254100-01);Similar to Lissencephaly typ e-1-like homology motif; CTLH, C-terminal to LisH motif; Nitrous oxide reductase, N-terminal; WD40-like; Quinonprotein alcohol d ehydrogenase-like. (Os01t0254100-02)
Parent gene strand	-
Alternative splicing	Os01g0254100_circ_g.17 Os01g0254100_circ_g.18
Support reads	4/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0254100-01:2 Os01t0254100-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAAACCTGAAAGATATTTCTCCACTTCATCCCACTCCCCAGCATGGACCTTCTCTTCAAAGTAC
TTCATGTTGAAGAAGAATCCTGACTCTTGCTCAAGCctgaaattctcaagagttagaagctgtg
ttatctctttatataactcctcgttgaatgtcgagaagactttaagatctttaacaagaatatc
tactgcct

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 294
Transcript exons: 8428423-8428536,8428669-8428848
CTGAAATTCTCAAGAGTTAGAAGCTGTGTTATCTCTTTATATAACTCCTCGTTGAATGTCGAGA
AGACTTTAAGATCTTTAACAAGAATATCTACTGCCTTTGCTCTATCATGCCTGTCAAGTGCTTC
CAAATACTTCTGCTTTCTGATCTCAAAGAATATCTTCATAGAATACCTATTGTCATCCACCTTG
GTGAAACCTGAAAGATATTTCTCCACTTCATCCCACTCCCCAGCATGGACCTTCTCTTCAAAGT
ACTTCATGTTGAAGAAGAATCCTGACTCTTGCTCAAGC
						
						Genomic sequence
						
							CTGAAATTCTCAAGAGTTAGAAGCTGTGTTATCTCTTTATATAACTCCTCGTTGAATGTCGAGA
AGACTTTAAGATCTTTAACAAGAATATCTACTGCCTTTGCTCTATCATGCCTGTTACAAAAAAA
ATATATAAATGAATATGAAAATTAGTAGAAGTTATATCTCACCCAGTGCCAAAAGGATGGAACA
CAAGTCCTTTTTTTGGTTGAAATGGGAACTTTCCCTTCAGATATGAAATTATACCTGTCAAGTG
CTTCCAAATACTTCTGCTTTCTGATCTCAAAGAATATCTTCATAGAATACCTATTGTCATCCAC
CTTGGTGAAACCTGAAAGATATTTCTCCACTTCATCCCACTCCCCAGCATGGACCTTCTCTTCA
AAGTACTTCATGTTGAAGAAGAATCCTGACTCTTGCTCAAGC

Sequences
Splice junction sequence	GAAACCTGAAAGATATTTCTCCACTTCATCCCACTCCCCAGCATGGACCTTCTCTTCAAAGTAC TTCATGTTGAAGAAGAATCCTGACTCTTGCTCAAGCctgaaattctcaagagttagaagctgtg ttatctctttatataactcctcgttgaatgtcgagaagactttaagatctttaacaagaatatc tactgcct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 294 Transcript exons: 8428423-8428536,8428669-8428848 CTGAAATTCTCAAGAGTTAGAAGCTGTGTTATCTCTTTATATAACTCCTCGTTGAATGTCGAGA AGACTTTAAGATCTTTAACAAGAATATCTACTGCCTTTGCTCTATCATGCCTGTCAAGTGCTTC CAAATACTTCTGCTTTCTGATCTCAAAGAATATCTTCATAGAATACCTATTGTCATCCACCTTG GTGAAACCTGAAAGATATTTCTCCACTTCATCCCACTCCCCAGCATGGACCTTCTCTTCAAAGT ACTTCATGTTGAAGAAGAATCCTGACTCTTGCTCAAGC
Genomic sequence	CTGAAATTCTCAAGAGTTAGAAGCTGTGTTATCTCTTTATATAACTCCTCGTTGAATGTCGAGA AGACTTTAAGATCTTTAACAAGAATATCTACTGCCTTTGCTCTATCATGCCTGTTACAAAAAAA ATATATAAATGAATATGAAAATTAGTAGAAGTTATATCTCACCCAGTGCCAAAAGGATGGAACA CAAGTCCTTTTTTTGGTTGAAATGGGAACTTTCCCTTCAGATATGAAATTATACCTGTCAAGTG CTTCCAAATACTTCTGCTTTCTGATCTCAAAGAATATCTTCATAGAATACCTATTGTCATCCAC CTTGGTGAAACCTGAAAGATATTTCTCCACTTCATCCCACTCCCCAGCATGGACCTTCTCTTCA AAGTACTTCATGTTGAAGAAGAATCCTGACTCTTGCTCAAGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.440384605

Conservation Information
Conserved circRNAs	NA
PMCS	0.440384605

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8428817-8428845(+)
8428819-8428425(-)
						
						Potential amino acid sequence
						
							MLKKNPDSCSSLKFSRVRSCVISLYNSSLNVEKTLRSLTRISTAFALSCLSSASKYFCFLISKN
IFIEYLLSSTLVKPERYFSTSSHSPAWTFSSKYFMLKKNPDSCSSLKFSRVRSCVISLYNSSLN
VEKTLRSLTRISTAFALSCLSSASKYFCFLISKNIFIEYLLSSTLVKPERYFSTSSHSPAWTFS
SKYFMLKKNPDSCSSLKFSRVRSCVISLYNSSLNVEKTLRSLTRISTAFALSCLSSASKYFCFL
ISKNIFIEYLLSSTLVKPERYFSTSSHSPAWTFSSKYFMLKKNPDSCSS(+)
MKYFEEKVHAGEWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDRHDRAKAVDILVKDL
KVFSTFNEELYKEITQLLTLENFRLEQESGFFFNMKYFEEKVHAGEWDEVEKYLSGFTKVDDNR
YSMKIFFEIRKQKYLEALDRHDRAKAVDILVKDLKVFSTFNEELYKEITQLLTLENFRLEQESG
FFFNMKYFEEKVHAGEWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDRHDRAKAVDIL
VKDLKVFSTFNEELYKEITQLLTLENFRLEQESGFFFNMKYFEEKVHAGEWDEVEKYLSGFTKV
DDNRYSMKIFFEIRKQKYLEALDRHDRAKAVDILVKDLKVFSTFNEELYKEITQLLTLENF(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8428817-8428845(+) 8428819-8428425(-)
Potential amino acid sequence	MLKKNPDSCSSLKFSRVRSCVISLYNSSLNVEKTLRSLTRISTAFALSCLSSASKYFCFLISKN IFIEYLLSSTLVKPERYFSTSSHSPAWTFSSKYFMLKKNPDSCSSLKFSRVRSCVISLYNSSLN VEKTLRSLTRISTAFALSCLSSASKYFCFLISKNIFIEYLLSSTLVKPERYFSTSSHSPAWTFS SKYFMLKKNPDSCSSLKFSRVRSCVISLYNSSLNVEKTLRSLTRISTAFALSCLSSASKYFCFL ISKNIFIEYLLSSTLVKPERYFSTSSHSPAWTFSSKYFMLKKNPDSCSS(+) MKYFEEKVHAGEWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDRHDRAKAVDILVKDL KVFSTFNEELYKEITQLLTLENFRLEQESGFFFNMKYFEEKVHAGEWDEVEKYLSGFTKVDDNR YSMKIFFEIRKQKYLEALDRHDRAKAVDILVKDLKVFSTFNEELYKEITQLLTLENFRLEQESG FFFNMKYFEEKVHAGEWDEVEKYLSGFTKVDDNRYSMKIFFEIRKQKYLEALDRHDRAKAVDIL VKDLKVFSTFNEELYKEITQLLTLENFRLEQESGFFFNMKYFEEKVHAGEWDEVEKYLSGFTKV DDNRYSMKIFFEIRKQKYLEALDRHDRAKAVDILVKDLKVFSTFNEELYKEITQLLTLENF(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0254100_circ_g.16

Address

Any questions?

Supported by