Plant Circular RNA Database

Detail information of Os12g0178700_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os12g0178700_circ_g.1
					
						ID in PlantcircBase
						osa_circ_010686
					
						Alias
						Os_ciR7546
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 3987165-3989370  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os12g0178700						
					
						Parent gene annotation
						
							Similar to ARC5 (ACCUMULATION AND REPLICATION OF CHLOROPLAST 5);
 GTP binding / GTPase. (Os12t0178700-01)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0178700-01:7

General Information
CircRNA Name	Os12g0178700_circ_g.1
ID in PlantcircBase	osa_circ_010686
Alias	Os_ciR7546
Organism	Oryza sativa
Position	chr12: 3987165-3989370 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os12g0178700
Parent gene annotation	Similar to ARC5 (ACCUMULATION AND REPLICATION OF CHLOROPLAST 5); GTP binding / GTPase. (Os12t0178700-01)
Parent gene strand	-
Alternative splicing	NA
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0178700-01:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CCCTAGTAATTGGAGGGCATCTAATACTTCCAACAACCAGACGAAATTCAGCCATTGCCCGATG
GTATTGTGCGCCACCGTAGAGACGCATTCCAGCATTccgcttcaattcgatctccagatctctc
ttcctctgcccaagtgaatggcgcagctgagtaacatcaaatatgctgtcaagatcatcttcga
aggctgat

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 943
Transcript exons: 3987165-3987329,3987413-3987745,3987825-3987882,3988468-
3988572,3988654-3988716,3989073-3989133,3989213-3989370
CCGCTTCAATTCGATCTCCAGATCTCTCTTCCTCTGCCCAAGTGAATGGCGCAGCTGAGTAACA
TCAAATATGCTGTCAAGATCATCTTCGAAGGCTGATTCAAGGTCTTCTCTTAGAAGCGCCGGTA
ATTTGTCAACAATCGGCATGAGGAGAAAGCAATTGAACTTAAGCTCTGTGGTGACAAGAAAATG
CTCTTTGATCCCATGGAAAATCTGGTGAACCAGTGCATACACAAGTCTTTCAGATGAGGGTGCC
AGCCTTCTGTTCCATAGTGTGCTGTCCAAGAGATCAACAAGCCTAGTCTCTGATGCAACAGTGG
GTGAATTTGCTTCTGAAGAATTACTTGATTTGAAGTCTCCCTTTGGCCTATCCTGCTTATTGTC
ATTCAAGCCAAAGGATTGCTCATGCAGCCCAGTCGAGTGGGCAGTAAGTGTGTTCACGGATAAT
TGCTCTTGTGCAACAAAGGAATCAAAGAAGTGTCTCAATCCGGCACGGTTCTTATTGTGAAGGG
ACCAAGTGACATAACGAGTGGTGCTCACCAAATCCTCCATACATCTTTCACGACATGATTGCTC
AGTAGATTCATGCAAACTCTATCAAATTGACAGCTTTGACACGCTTCACAAGTACATCATGGCT
GCTACAGTTCTCACCATCTTTCTCCAACAGAAAAGCAGATATCGGAATCAGTCTCTTCATTATG
TATAATAACCTAAAACCCAGCTGGTGAAGATAAGGCTCAAATGTGTCACGTGCTTTTGCCACAG
CTAACACACAAGCAGTCCTGGAGTAGTTTGTCCCATCATGAATATCTTCCACACCACAAGCATT
GACAATTTCCTCCCTAGTAATTGGAGGGCATCTAATACTTCCAACAACCAGACGAAATTCAGCC
ATTGCCCGATGGTATTGTGCGCCACCGTAGAGACGCATTCCAGCATT
						
					

						Genomic sequence
						
							CCGCTTCAATTCGATCTCCAGATCTCTCTTCCTCTGCCCAAGTGAATGGCGCAGCTGAGTAACA
TCAAATATGCTGTCAAGATCATCTTCGAAGGCTGATTCAAGGTCTTCTCTTAGAAGCGCCGGTA
ATTTGTCAACAATCGGCATGAGGAGAAAGCAATTGAACTGCAATTTTGATGAGCAAAACAAGAT
ATAAACAATAAACTTCATATACTCGTTAAGGAGTGCAGGATTTACTGCAGGGAGACCTTAAGCT
CTGTGGTGACAAGAAAATGCTCTTTGATCCCATGGAAAATCTGGTGAACCAGTGCATACACAAG
TCTTTCAGATGAGGGTGCCAGCCTTCTGTTCCATAGTGTGCTGTCCAAGAGATCAACAAGCCTA
GTCTCTGATGCAACAGTGGGTGAATTTGCTTCTGAAGAATTACTTGATTTGAAGTCTCCCTTTG
GCCTATCCTGCTTATTGTCATTCAAGCCAAAGGATTGCTCATGCAGCCCAGTCGAGTGGGCAGT
AAGTGTGTTCACGGATAATTGCTCTTGTGCAACAAAGGAATCAAAGAAGTGTCTCAATCCGGCA
CGGTTCTATACAATGTAGCAAATCATTAGGAATGCGACGAATCCCTCGAAAGATACTAGTTCTG
ATTAATATTAATAACTGTACCTTATTGTGAAGGGACCAAGTGACATAACGAGTGGTGCTCACCA
AATCCTCCATACATCTGCATTACACATTTCAACATGCAGATAAATCATGCCATCAGGTACAAAC
AATATGCATGAATGCATCATGCCATGAACCATCAGGTACAAACAATATGCATGAATGCATCATG
CCATGAAGATTCAGGAGAAATCTGGACAATAACAGAAAGTTTTACTTTGACACAACACTAAATT
CTGTGTCATATTTACATGAGACAAACCACTAAAATCACATTTTTCAAATATGAATATGCTCTGG
AACGCACAAAATTTAATGCCATGAGATCAAAAAGGAGGGAAGACATGACAAAGATACGGATAAC
TCATTCAGTTCACTTCATTTGCACTTTATTATTCAATGATATTATAATTCAGCTGATACATTTC
TAAGTAGAACCACTATTAAAAAAAAACTAACTAGTTTGCCAACACCATAAAATACTGTATGCCT
GATAGCACAATAATCAACTAGGGAAATGCATTTCATTTGATATCAAAGAGTTTGTTGAACTGAG
TTTGCAAGACCACTTGAACAAGAGTTGTACACTAGAAGGGAAACAATTATAAAGGTATTGAAAT
CAAGGATGCTCTTACAGTATTACCTTTCACGACATGATTGCTCAGTAGATTCATGCAAACTCTA
TCAAATTGACAGCTTTGACACGCTTCACAAGTACATCATGGCTGCTACAGTTCTCACCATCTTT
CTGTTGGAATTCTATCACGGTATTACAAAAGAAAAGAGCAGAATACTAGTTGAACTGAATAACT
TCAGGATTAATTAGTACCTCCAACAGAAAAGCAGATATCGGAATCAGTCTCTTCATTATGTATA
ATAACCTAAAACCCAGCTGCACATAATTAAGGATAGATTTTAATCGCAGAAAGGGATTAAGCAG
AGGAAATATCACCCCTCATACAGACCACAACAAATTAATTCAATTACTAAATTTGTTTATACAT
CATGTTACATTTTTTATTGATTTCACACATTTTCTGTGGAACCCTGGCACACTCTAGTTCTGAA
CTTGATCCAAAAAATGATGGGACGTGCATGAAAAAAATCGGTTGATGATTTTATTGACATAATC
ACACAAAAGCAAGTACTATTCCAGTAGCTCAAGTGTGGAATAACAATCATGAAAGCTGAACAGG
ACCATCTAAATGTGATTATTAATGTGCCCACCCATGAGGTAAGCTAACCAACCTGGTGAAGATA
AGGCTCAAATGTGTCACGTGCTTTTGCCACAGCTAACACACAAGCAGTCCTAACAAAAAAAGTT
GAAAATGAGTAAATGTGCTATATAAATATAGTCAAAGTATGCCTGATATATGGAATGAGACTAC
CTGGAGTAGTTTGTCCCATCATGAATATCTTCCACACCACAAGCATTGACAATTTCCTCCCTAG
TAATTGGAGGGCATCTAATACTTCCAACAACCAGACGAAATTCAGCCATTGCCCGATGGTATTG
TGCGCCACCGTAGAGACGCATTCCAGCATT
						
					

			
		

Sequences
Splice junction sequence	CCCTAGTAATTGGAGGGCATCTAATACTTCCAACAACCAGACGAAATTCAGCCATTGCCCGATG GTATTGTGCGCCACCGTAGAGACGCATTCCAGCATTccgcttcaattcgatctccagatctctc ttcctctgcccaagtgaatggcgcagctgagtaacatcaaatatgctgtcaagatcatcttcga aggctgat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 943 Transcript exons: 3987165-3987329,3987413-3987745,3987825-3987882,3988468- 3988572,3988654-3988716,3989073-3989133,3989213-3989370 CCGCTTCAATTCGATCTCCAGATCTCTCTTCCTCTGCCCAAGTGAATGGCGCAGCTGAGTAACA TCAAATATGCTGTCAAGATCATCTTCGAAGGCTGATTCAAGGTCTTCTCTTAGAAGCGCCGGTA ATTTGTCAACAATCGGCATGAGGAGAAAGCAATTGAACTTAAGCTCTGTGGTGACAAGAAAATG CTCTTTGATCCCATGGAAAATCTGGTGAACCAGTGCATACACAAGTCTTTCAGATGAGGGTGCC AGCCTTCTGTTCCATAGTGTGCTGTCCAAGAGATCAACAAGCCTAGTCTCTGATGCAACAGTGG GTGAATTTGCTTCTGAAGAATTACTTGATTTGAAGTCTCCCTTTGGCCTATCCTGCTTATTGTC ATTCAAGCCAAAGGATTGCTCATGCAGCCCAGTCGAGTGGGCAGTAAGTGTGTTCACGGATAAT TGCTCTTGTGCAACAAAGGAATCAAAGAAGTGTCTCAATCCGGCACGGTTCTTATTGTGAAGGG ACCAAGTGACATAACGAGTGGTGCTCACCAAATCCTCCATACATCTTTCACGACATGATTGCTC AGTAGATTCATGCAAACTCTATCAAATTGACAGCTTTGACACGCTTCACAAGTACATCATGGCT GCTACAGTTCTCACCATCTTTCTCCAACAGAAAAGCAGATATCGGAATCAGTCTCTTCATTATG TATAATAACCTAAAACCCAGCTGGTGAAGATAAGGCTCAAATGTGTCACGTGCTTTTGCCACAG CTAACACACAAGCAGTCCTGGAGTAGTTTGTCCCATCATGAATATCTTCCACACCACAAGCATT GACAATTTCCTCCCTAGTAATTGGAGGGCATCTAATACTTCCAACAACCAGACGAAATTCAGCC ATTGCCCGATGGTATTGTGCGCCACCGTAGAGACGCATTCCAGCATT
Genomic sequence	CCGCTTCAATTCGATCTCCAGATCTCTCTTCCTCTGCCCAAGTGAATGGCGCAGCTGAGTAACA TCAAATATGCTGTCAAGATCATCTTCGAAGGCTGATTCAAGGTCTTCTCTTAGAAGCGCCGGTA ATTTGTCAACAATCGGCATGAGGAGAAAGCAATTGAACTGCAATTTTGATGAGCAAAACAAGAT ATAAACAATAAACTTCATATACTCGTTAAGGAGTGCAGGATTTACTGCAGGGAGACCTTAAGCT CTGTGGTGACAAGAAAATGCTCTTTGATCCCATGGAAAATCTGGTGAACCAGTGCATACACAAG TCTTTCAGATGAGGGTGCCAGCCTTCTGTTCCATAGTGTGCTGTCCAAGAGATCAACAAGCCTA GTCTCTGATGCAACAGTGGGTGAATTTGCTTCTGAAGAATTACTTGATTTGAAGTCTCCCTTTG GCCTATCCTGCTTATTGTCATTCAAGCCAAAGGATTGCTCATGCAGCCCAGTCGAGTGGGCAGT AAGTGTGTTCACGGATAATTGCTCTTGTGCAACAAAGGAATCAAAGAAGTGTCTCAATCCGGCA CGGTTCTATACAATGTAGCAAATCATTAGGAATGCGACGAATCCCTCGAAAGATACTAGTTCTG ATTAATATTAATAACTGTACCTTATTGTGAAGGGACCAAGTGACATAACGAGTGGTGCTCACCA AATCCTCCATACATCTGCATTACACATTTCAACATGCAGATAAATCATGCCATCAGGTACAAAC AATATGCATGAATGCATCATGCCATGAACCATCAGGTACAAACAATATGCATGAATGCATCATG CCATGAAGATTCAGGAGAAATCTGGACAATAACAGAAAGTTTTACTTTGACACAACACTAAATT CTGTGTCATATTTACATGAGACAAACCACTAAAATCACATTTTTCAAATATGAATATGCTCTGG AACGCACAAAATTTAATGCCATGAGATCAAAAAGGAGGGAAGACATGACAAAGATACGGATAAC TCATTCAGTTCACTTCATTTGCACTTTATTATTCAATGATATTATAATTCAGCTGATACATTTC TAAGTAGAACCACTATTAAAAAAAAACTAACTAGTTTGCCAACACCATAAAATACTGTATGCCT GATAGCACAATAATCAACTAGGGAAATGCATTTCATTTGATATCAAAGAGTTTGTTGAACTGAG TTTGCAAGACCACTTGAACAAGAGTTGTACACTAGAAGGGAAACAATTATAAAGGTATTGAAAT CAAGGATGCTCTTACAGTATTACCTTTCACGACATGATTGCTCAGTAGATTCATGCAAACTCTA TCAAATTGACAGCTTTGACACGCTTCACAAGTACATCATGGCTGCTACAGTTCTCACCATCTTT CTGTTGGAATTCTATCACGGTATTACAAAAGAAAAGAGCAGAATACTAGTTGAACTGAATAACT TCAGGATTAATTAGTACCTCCAACAGAAAAGCAGATATCGGAATCAGTCTCTTCATTATGTATA ATAACCTAAAACCCAGCTGCACATAATTAAGGATAGATTTTAATCGCAGAAAGGGATTAAGCAG AGGAAATATCACCCCTCATACAGACCACAACAAATTAATTCAATTACTAAATTTGTTTATACAT CATGTTACATTTTTTATTGATTTCACACATTTTCTGTGGAACCCTGGCACACTCTAGTTCTGAA CTTGATCCAAAAAATGATGGGACGTGCATGAAAAAAATCGGTTGATGATTTTATTGACATAATC ACACAAAAGCAAGTACTATTCCAGTAGCTCAAGTGTGGAATAACAATCATGAAAGCTGAACAGG ACCATCTAAATGTGATTATTAATGTGCCCACCCATGAGGTAAGCTAACCAACCTGGTGAAGATA AGGCTCAAATGTGTCACGTGCTTTTGCCACAGCTAACACACAAGCAGTCCTAACAAAAAAAGTT GAAAATGAGTAAATGTGCTATATAAATATAGTCAAAGTATGCCTGATATATGGAATGAGACTAC CTGGAGTAGTTTGTCCCATCATGAATATCTTCCACACCACAAGCATTGACAATTTCCTCCCTAG TAATTGGAGGGCATCTAATACTTCCAACAACCAGACGAAATTCAGCCATTGCCCGATGGTATTG TGCGCCACCGTAGAGACGCATTCCAGCATT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.369316714

Conservation Information
Conserved circRNAs	NA
PMCS	0.369316714

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							3989234-3987206(+)
3987880-3988497(-)
						
						Potential amino acid sequence
						
							MNIFHTTSIDNFLPSNWRASNTSNNQTKFSHCPMVLCATVETHSSIPLQFDLQISLPLPK*(+)
MEDLVSTTRYVTWSLHNKNRAGLRHFFDSFVAQEQLSVNTLTAHSTGLHEQSFGLNDNKQDRPK
GDFKSSNSSEANSPTVASETRLVDLLDSTLWNRRLAPSSERLVYALVHQIFHGIKEHFLVTTEL
KFNCFLLMPIVDKLPALLREDLESAFEDDLDSIFDVTQLRHSLGQRKRDLEIELKRNAGMRLYG
GAQYHRAMAEFRLVVGSIRCPPITREEIVNACGVEDIHDGTNYSRTACVLAVAKARDTFEPYLH
QLGFRLLYIMKRLIPISAFLLEKDGENCSSHDVLVKRVKAVNLIEFA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	3989234-3987206(+) 3987880-3988497(-)
Potential amino acid sequence	MNIFHTTSIDNFLPSNWRASNTSNNQTKFSHCPMVLCATVETHSSIPLQFDLQISLPLPK(+) MEDLVSTTRYVTWSLHNKNRAGLRHFFDSFVAQEQLSVNTLTAHSTGLHEQSFGLNDNKQDRPK GDFKSSNSSEANSPTVASETRLVDLLDSTLWNRRLAPSSERLVYALVHQIFHGIKEHFLVTTEL KFNCFLLMPIVDKLPALLREDLESAFEDDLDSIFDVTQLRHSLGQRKRDLEIELKRNAGMRLYG GAQYHRAMAEFRLVVGSIRCPPITREEIVNACGVEDIHDGTNYSRTACVLAVAKARDTFEPYLH QLGFRLLYIMKRLIPISAFLLEKDGENCSSHDVLVKRVKAVNLIEFA(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os12g0178700_circ_g.1

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