Plant Circular RNA Database

Detail information of AT5G13000_circ_g.25

			
						 General Information
					
						CircRNA Name
						AT5G13000_circ_g.25
					
						ID in PlantcircBase
						ath_circ_037878
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 4119981-4120694  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							AT5G13000						
					
						Parent gene annotation
						
							Callose synthase 3
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT5G13000_circ_g.22 AT5G13000_circ_g.23 AT5G13000_circ_g.24 AT5G13000_circ_g.26 						
					
						Support reads
						1
					
						Tissues
						seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G13000.2:4
AT5G13000.1:4

General Information
CircRNA Name	AT5G13000_circ_g.25
ID in PlantcircBase	ath_circ_037878
Alias	NA
Organism	Arabidpsis thaliana
Position	chr5: 4119981-4120694 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	AT5G13000
Parent gene annotation	Callose synthase 3
Parent gene strand	-
Alternative splicing	AT5G13000_circ_g.22 AT5G13000_circ_g.23 AT5G13000_circ_g.24 AT5G13000_circ_g.26
Support reads	1
Tissues	seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G13000.2:4 AT5G13000.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCAAAGCTTGAATATACTTCTTATAGTAGTGTTGATAAAAGCTTTGCATTTCACGGGCATCACT
TTTCTTAACCCTGCCCATCAGTGTTGGATCATGTTCctgaaatccaaacatttcttgaagccaa
tcaagcatgtcttcatctttctttttcttgtgaccttctggccaaggaagacctctagtattgc
ggagagca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 447
Transcript exons: 4119981-4120097,4120172-4120282,4120366-4120459,4120570-
4120694
CTGAAATCCAAACATTTCTTGAAGCCAATCAAGCATGTCTTCATCTTTCTTTTTCTTGTGACCT
TCTGGCCAAGGAAGACCTCTAGTATTGCGGAGAGCAAGAACAGCAGCTTGGATCTCAGGATATC
TCATAATCGCTTGATTGGCACTATCAGGATCGAGAGGTAGGATATTATAGGGGACATACAACTG
TGTCTTTTCTGCAACCTTATCTTGAGCTTCCAGAATCTCCCGGTCAACCTCAATGGACTGTGTC
AGATTAACAGCCTTCAACACCTCAAACAAAACATTGGCAGTTTGGTATGCCTTTGTCAGCTGGG
CACGGTCAGCCTTATCAGCAGCATTGTGCAAAGCTTGAATATACTTCTTATAGTAGTGTTGATA
AAAGCTTTGCATTTCACGGGCATCACTTTTCTTAACCCTGCCCATCAGTGTTGGATCATGTTC
						
						Genomic sequence
						
							CTGAAATCCAAACATTTCTTGAAGCCAATCAAGCATGTCTTCATCTTTCTTTTTCTTGTGACCT
TCTGGCCAAGGAAGACCTCTAGTATTGCGGAGAGCAAGAACAGCAGCTTGGATCTGCAGTAAGG
ATTACATCACCCGCTGATAACATATTACAAATAATAACAATGCCCAACAAGAAGAGGACTAACC
TCAGGATATCTCATAATCGCTTGATTGGCACTATCAGGATCGAGAGGTAGGATATTATAGGGGA
CATACAACTGTGTCTTTTCTGCAACCTTATCTTGAGCTTCCAGAATCTGGAACCATTCAACTTC
ATTGTAAAGTTGCACAAGAGATGGAAAAAAAAAAGAGACAGGACTAGAAGGTCACAATGCCTTA
CCTCCCGGTCAACCTCAATGGACTGTGTCAGATTAACAGCCTTCAACACCTCAAACAAAACATT
GGCAGTTTGGTATGCCTTTGTCAGCTGGGCACTGAAAATTAACAGCACTTCAAAGTATCATTAA
GAGAATTTAGTGCTCTCAAACACTGGCAAAACACAAATTAGCATCTTAAAAAGACAATTTAAGT
AAGTTAGACATACCGGTCAGCCTTATCAGCAGCATTGTGCAAAGCTTGAATATACTTCTTATAG
TAGTGTTGATAAAAGCTTTGCATTTCACGGGCATCACTTTTCTTAACCCTGCCCATCAGTGTTG
GATCATGTTC

Sequences
Splice junction sequence	GCAAAGCTTGAATATACTTCTTATAGTAGTGTTGATAAAAGCTTTGCATTTCACGGGCATCACT TTTCTTAACCCTGCCCATCAGTGTTGGATCATGTTCctgaaatccaaacatttcttgaagccaa tcaagcatgtcttcatctttctttttcttgtgaccttctggccaaggaagacctctagtattgc ggagagca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 447 Transcript exons: 4119981-4120097,4120172-4120282,4120366-4120459,4120570- 4120694 CTGAAATCCAAACATTTCTTGAAGCCAATCAAGCATGTCTTCATCTTTCTTTTTCTTGTGACCT TCTGGCCAAGGAAGACCTCTAGTATTGCGGAGAGCAAGAACAGCAGCTTGGATCTCAGGATATC TCATAATCGCTTGATTGGCACTATCAGGATCGAGAGGTAGGATATTATAGGGGACATACAACTG TGTCTTTTCTGCAACCTTATCTTGAGCTTCCAGAATCTCCCGGTCAACCTCAATGGACTGTGTC AGATTAACAGCCTTCAACACCTCAAACAAAACATTGGCAGTTTGGTATGCCTTTGTCAGCTGGG CACGGTCAGCCTTATCAGCAGCATTGTGCAAAGCTTGAATATACTTCTTATAGTAGTGTTGATA AAAGCTTTGCATTTCACGGGCATCACTTTTCTTAACCCTGCCCATCAGTGTTGGATCATGTTC
Genomic sequence	CTGAAATCCAAACATTTCTTGAAGCCAATCAAGCATGTCTTCATCTTTCTTTTTCTTGTGACCT TCTGGCCAAGGAAGACCTCTAGTATTGCGGAGAGCAAGAACAGCAGCTTGGATCTGCAGTAAGG ATTACATCACCCGCTGATAACATATTACAAATAATAACAATGCCCAACAAGAAGAGGACTAACC TCAGGATATCTCATAATCGCTTGATTGGCACTATCAGGATCGAGAGGTAGGATATTATAGGGGA CATACAACTGTGTCTTTTCTGCAACCTTATCTTGAGCTTCCAGAATCTGGAACCATTCAACTTC ATTGTAAAGTTGCACAAGAGATGGAAAAAAAAAAGAGACAGGACTAGAAGGTCACAATGCCTTA CCTCCCGGTCAACCTCAATGGACTGTGTCAGATTAACAGCCTTCAACACCTCAAACAAAACATT GGCAGTTTGGTATGCCTTTGTCAGCTGGGCACTGAAAATTAACAGCACTTCAAAGTATCATTAA GAGAATTTAGTGCTCTCAAACACTGGCAAAACACAAATTAGCATCTTAAAAAGACAATTTAAGT AAGTTAGACATACCGGTCAGCCTTATCAGCAGCATTGTGCAAAGCTTGAATATACTTCTTATAG TAGTGTTGATAAAAGCTTTGCATTTCACGGGCATCACTTTTCTTAACCCTGCCCATCAGTGTTG GATCATGTTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.275625756

Conservation Information
Conserved circRNAs	NA
PMCS	0.275625756

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4120677-4119983(-)
						
						Potential amino acid sequence
						
							MGRVKKSDAREMQSFYQHYYKKYIQALHNAADKADRAQLTKAYQTANVLFEVLKAVNLTQSIEV
DREILEAQDKVAEKTQLYVPYNILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKK
DEDMLDWLQEMFGFQEHDPTLMGRVKKSDAREMQSFYQHYYKKYIQALHNAADKADRAQLTKAY
QTANVLFEVLKAVNLTQSIEVDREILEAQDKVAEKTQLYVPYNILPLDPDSANQAIMRYPEIQA
AVLALRNTRGLPWPEGHKKKKDEDMLDWLQEMFGFQEHDPTLMGRVKKSDAREMQSFYQHYYKK
YIQALHNAADKADRAQLTKAYQTANVLFEVLKAVNLTQSIEVDREILEAQDKVAEKTQLYVPYN
ILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKKDEDMLDWLQEMFGFQEHDPTLM
GRVKKSDAREMQSFYQHYYKKYIQALHNAADKADRAQLTKAYQTANVLFEVLKAVNLTQSIEVD
REILEAQDKVAEKTQLYVPYNILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKKD
EDMLDWLQEMFGFQ(-)
						
						Sponge-miRNAs
						
							ath-miR829-5p 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	4120677-4119983(-)
Potential amino acid sequence	MGRVKKSDAREMQSFYQHYYKKYIQALHNAADKADRAQLTKAYQTANVLFEVLKAVNLTQSIEV DREILEAQDKVAEKTQLYVPYNILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKK DEDMLDWLQEMFGFQEHDPTLMGRVKKSDAREMQSFYQHYYKKYIQALHNAADKADRAQLTKAY QTANVLFEVLKAVNLTQSIEVDREILEAQDKVAEKTQLYVPYNILPLDPDSANQAIMRYPEIQA AVLALRNTRGLPWPEGHKKKKDEDMLDWLQEMFGFQEHDPTLMGRVKKSDAREMQSFYQHYYKK YIQALHNAADKADRAQLTKAYQTANVLFEVLKAVNLTQSIEVDREILEAQDKVAEKTQLYVPYN ILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKKDEDMLDWLQEMFGFQEHDPTLM GRVKKSDAREMQSFYQHYYKKYIQALHNAADKADRAQLTKAYQTANVLFEVLKAVNLTQSIEVD REILEAQDKVAEKTQLYVPYNILPLDPDSANQAIMRYPEIQAAVLALRNTRGLPWPEGHKKKKD EDMLDWLQEMFGFQ(-)
Sponge-miRNAs	ath-miR829-5p
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT5G13000_circ_g.25

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