Plant Circular RNA Database

Detail information of orai.004G057700_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.004G057700_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000336
					
						Alias
						Chr04:5541500|5543637
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr04: 5541500-5543637  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.004G057700						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						8/2
					
						Tissues
						leaf/ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.004G057700.3:6
Gorai.004G057700.2:6
Gorai.004G057700.1:6
Gorai.004G057700.4:6

General Information
CircRNA Name	orai.004G057700_circ_g.1
ID in PlantcircBase	gra_circ_000336
Alias	Chr04:5541500\|5543637
Organism	Gossypium raimondii
Position	chrChr04: 5541500-5543637 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.004G057700
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	8/2
Tissues	leaf/ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.004G057700.3:6 Gorai.004G057700.2:6 Gorai.004G057700.1:6 Gorai.004G057700.4:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGAACTCTCACAGTCCCAATTATTATCTGCTTTGGATGGAGCAAATATGGTCTATTTTGATGGA
AGACTGTACGAAACTGCTTTAATTATTGCAAAAGAGggcccaaatgtcatccgaatcgcttcca
ccacttcctgaacatcgcatcgtcgtacgctttttttatttttaaattgcattattgtctctat
cgtatatt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 499
Transcript exons: 5541500-5541551,5541678-5541767,5542355-5542432,5542541-
5542615,5543064-5543116,5543487-5543637
GGCCCAAATGTCATCCGAATCGCTTCCACCACTTCCTGAACATCGCATCGTCCTAGGGTGTGGG
TCTGTCGGAGTGGATTTCTTGGCCTCCGTCTCTTCTTATCCTAAACCTGATGACAAAATCCGAA
GCACTAGCTTAAAGGTTCAGGGAGGTGGGAATGCTGGAAATGCCTTAACTTGTGTTGCTCGTTT
AGGATTAAATCCACGACTAATTTCTAAGGTTGCCAATGATGCCCAAGGCAAGAGTATATTGGAG
GAGCTTGAATCTGATGGAGTCGACACTTCATTCTTTATTGTTTCTGAGGAGGGGAATTCGCCAT
TTACTTATATAATTGTTGACAACGAAACGAAAACTCGCACCTGTATTCACACCCCTGGTTATCC
ACCCTTGATACCAGATGAACTCTCACAGTCCCAATTATTATCTGCTTTGGATGGAGCAAATATG
GTCTATTTTGATGGAAGACTGTACGAAACTGCTTTAATTATTGCAAAAGAG
						
					

						Genomic sequence
						
							GGCCCAAATGTCATCCGAATCGCTTCCACCACTTCCTGAACATCGCATCGTCGTACGCTTTTTT
TATTTTTAAATTGCATTATTGTCTCTATCGTATATTCATACCCCCTCTTTGTTTTTGTTAAAGC
ATTATCATAAAATTGATGAATGTCTTCAATTTTGTTAACATGATCGACAGCTAGGGTGTGGGTC
TGTCGGAGTGGATTTCTTGGCCTCCGTCTCTTCTTATCCTAAACCTGATGACAAAATCCGAAGC
ACTAGCTTAAAGGTTCTTCTTTTCTAAAAACCCTTGCTTCCTACTTTTATTTACTTAATCTACT
GATGATTGACTTTCTTTGTAAAGCTTTGGGGTATAGAGTATGTGTCTGACATGGGTATTTTGGT
TCGACACGATTATCTTATTCGGACCTTCTTTTAGTGTATTTTAAGTGAGCAATGTAGTTCTTAT
TTGATCAATGTTTCAATTTGCCCTTCTCTGTTCAGTTTCGTTGTAGAACCTCGTAGTAGGTTAT
GTTACTTGGGCTCATGAGTGTTGGGTATGTACATGTGTCCAATACGAATATTTTTGTCTATCAA
TGTTTTAATTTGCTCTTCCGTCTTCCATTTCTTTGTAGAACCTCCCAGTAGTTTGTAAGTCTCA
AGTATGGATATGTCTCCAACATGAGTATTTTCATTTTATTTTCTAAGTTTTTAGCTTGTACTTG
GAGAATCCTTGGAGGGTCATGTTTTCATATCCAAGTTTAAATATGCATTGGGCACACTTGTTGT
AAGAGAAGGATACTTAAGGAAAATTGTAAGAGCTTGAGCTATATAACTAGTAGGTTTAATTGAT
TAGTTTAGCATTTTTTTCCACAGGTTCAGGGAGGTGGGAATGCTGGAAATGCCTTAACTTGTGT
TGCTCGTTTAGGATTAAATCCACGACTAATTTCTAAGGTCTTACATATCTTTGTTCTTCTTTAT
TTTCTCTATCTACTTCAATTCCACCGTGAAATTTGACTGTTGAATGAGATAACACTTTTATTGC
ATCATATAAATCATTAGGTTGCCAATGATGCCCAAGGCAAGAGTATATTGGAGGAGCTTGAATC
TGATGGAGTCGACACTTCATTCTTTATTGTAAGGCTTTGAGATATTTTATGGTGCTTACTTATG
GGAAAGATCCATAGCCTACTATTATCATTGCTTTTATCTTGATTGATATAAGAAGGTTATTTGC
CTTGAAGCTTTATTCCATTGGTATTTTTGAATGTTTGGATATTCGATTCTAGAAATAATGCTCC
ACCCGGATTTATGCAATATAGTTGGATCATTTGCGTTTGTCAATCATGACTTGTCCAGACTCTA
GAAGTTAGAAGCCAAGAAAAGAACCTAGGAACTTGGACTGTCATATAGTAGAGACCGACATGGG
AAGGACTGTATTTTATCATACATCACATCATAGATGCCTAGATGAGCTTTGTGCACTGGGCAGC
CTTTTTTTTTCATATAAGACATATTTTACTGCCATTAAATGTCTCATTACAACCTGGACTCCCG
CTTTCTGAACTAATTGTGTGGATTTCAGGTTTCTGAGGAGGGGAATTCGCCATTTACTTATATA
ATTGTTGACAACGAAACGTGAGCACCTGTTGCCTTATTCCATGCATTATATAATCAGTTATCCT
TTCAGTAAGAAGTGTATTTCCTTGAAAAATCCTTCTTTTCTCTCCGATTCTGTGTATAGGCATT
CGAGATCTGCCTATAGCAGGTTTAAAGCATGGAAAATTATCTTCTTCAGAGTGGCTTAGATGGC
TTAAGGTCACCTTTGACCAGCTCTTTCCATCCTTTATTTAAAATAGTATTTATTTACATTTTCT
TTGGGTGTATATATAAGCCGCAACTCTTAACACTTGTGCCAAGAACCCAAAGAAAAAAAAGAAG
TTTTGCTTATGTTAAAAAATGAAAATAGAAAGCATGTGTATCCAATCAGTTGACACTAAACTTT
CAGGAAAACTCGCACCTGTATTCACACCCCTGGTTATCCACCCTTGATACCAGATGAACTCTCA
CAGTCCCAATTATTATCTGCTTTGGATGGAGCAAATATGGTCTATTTTGATGGAAGACTGTACG
AAACTGCTTTAATTATTGCAAAAGAG
						
					

			
		

Sequences
Splice junction sequence	TGAACTCTCACAGTCCCAATTATTATCTGCTTTGGATGGAGCAAATATGGTCTATTTTGATGGA AGACTGTACGAAACTGCTTTAATTATTGCAAAAGAGggcccaaatgtcatccgaatcgcttcca ccacttcctgaacatcgcatcgtcgtacgctttttttatttttaaattgcattattgtctctat cgtatatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 499 Transcript exons: 5541500-5541551,5541678-5541767,5542355-5542432,5542541- 5542615,5543064-5543116,5543487-5543637 GGCCCAAATGTCATCCGAATCGCTTCCACCACTTCCTGAACATCGCATCGTCCTAGGGTGTGGG TCTGTCGGAGTGGATTTCTTGGCCTCCGTCTCTTCTTATCCTAAACCTGATGACAAAATCCGAA GCACTAGCTTAAAGGTTCAGGGAGGTGGGAATGCTGGAAATGCCTTAACTTGTGTTGCTCGTTT AGGATTAAATCCACGACTAATTTCTAAGGTTGCCAATGATGCCCAAGGCAAGAGTATATTGGAG GAGCTTGAATCTGATGGAGTCGACACTTCATTCTTTATTGTTTCTGAGGAGGGGAATTCGCCAT TTACTTATATAATTGTTGACAACGAAACGAAAACTCGCACCTGTATTCACACCCCTGGTTATCC ACCCTTGATACCAGATGAACTCTCACAGTCCCAATTATTATCTGCTTTGGATGGAGCAAATATG GTCTATTTTGATGGAAGACTGTACGAAACTGCTTTAATTATTGCAAAAGAG
Genomic sequence	GGCCCAAATGTCATCCGAATCGCTTCCACCACTTCCTGAACATCGCATCGTCGTACGCTTTTTT TATTTTTAAATTGCATTATTGTCTCTATCGTATATTCATACCCCCTCTTTGTTTTTGTTAAAGC ATTATCATAAAATTGATGAATGTCTTCAATTTTGTTAACATGATCGACAGCTAGGGTGTGGGTC TGTCGGAGTGGATTTCTTGGCCTCCGTCTCTTCTTATCCTAAACCTGATGACAAAATCCGAAGC ACTAGCTTAAAGGTTCTTCTTTTCTAAAAACCCTTGCTTCCTACTTTTATTTACTTAATCTACT GATGATTGACTTTCTTTGTAAAGCTTTGGGGTATAGAGTATGTGTCTGACATGGGTATTTTGGT TCGACACGATTATCTTATTCGGACCTTCTTTTAGTGTATTTTAAGTGAGCAATGTAGTTCTTAT TTGATCAATGTTTCAATTTGCCCTTCTCTGTTCAGTTTCGTTGTAGAACCTCGTAGTAGGTTAT GTTACTTGGGCTCATGAGTGTTGGGTATGTACATGTGTCCAATACGAATATTTTTGTCTATCAA TGTTTTAATTTGCTCTTCCGTCTTCCATTTCTTTGTAGAACCTCCCAGTAGTTTGTAAGTCTCA AGTATGGATATGTCTCCAACATGAGTATTTTCATTTTATTTTCTAAGTTTTTAGCTTGTACTTG GAGAATCCTTGGAGGGTCATGTTTTCATATCCAAGTTTAAATATGCATTGGGCACACTTGTTGT AAGAGAAGGATACTTAAGGAAAATTGTAAGAGCTTGAGCTATATAACTAGTAGGTTTAATTGAT TAGTTTAGCATTTTTTTCCACAGGTTCAGGGAGGTGGGAATGCTGGAAATGCCTTAACTTGTGT TGCTCGTTTAGGATTAAATCCACGACTAATTTCTAAGGTCTTACATATCTTTGTTCTTCTTTAT TTTCTCTATCTACTTCAATTCCACCGTGAAATTTGACTGTTGAATGAGATAACACTTTTATTGC ATCATATAAATCATTAGGTTGCCAATGATGCCCAAGGCAAGAGTATATTGGAGGAGCTTGAATC TGATGGAGTCGACACTTCATTCTTTATTGTAAGGCTTTGAGATATTTTATGGTGCTTACTTATG GGAAAGATCCATAGCCTACTATTATCATTGCTTTTATCTTGATTGATATAAGAAGGTTATTTGC CTTGAAGCTTTATTCCATTGGTATTTTTGAATGTTTGGATATTCGATTCTAGAAATAATGCTCC ACCCGGATTTATGCAATATAGTTGGATCATTTGCGTTTGTCAATCATGACTTGTCCAGACTCTA GAAGTTAGAAGCCAAGAAAAGAACCTAGGAACTTGGACTGTCATATAGTAGAGACCGACATGGG AAGGACTGTATTTTATCATACATCACATCATAGATGCCTAGATGAGCTTTGTGCACTGGGCAGC CTTTTTTTTTCATATAAGACATATTTTACTGCCATTAAATGTCTCATTACAACCTGGACTCCCG CTTTCTGAACTAATTGTGTGGATTTCAGGTTTCTGAGGAGGGGAATTCGCCATTTACTTATATA ATTGTTGACAACGAAACGTGAGCACCTGTTGCCTTATTCCATGCATTATATAATCAGTTATCCT TTCAGTAAGAAGTGTATTTCCTTGAAAAATCCTTCTTTTCTCTCCGATTCTGTGTATAGGCATT CGAGATCTGCCTATAGCAGGTTTAAAGCATGGAAAATTATCTTCTTCAGAGTGGCTTAGATGGC TTAAGGTCACCTTTGACCAGCTCTTTCCATCCTTTATTTAAAATAGTATTTATTTACATTTTCT TTGGGTGTATATATAAGCCGCAACTCTTAACACTTGTGCCAAGAACCCAAAGAAAAAAAAGAAG TTTTGCTTATGTTAAAAAATGAAAATAGAAAGCATGTGTATCCAATCAGTTGACACTAAACTTT CAGGAAAACTCGCACCTGTATTCACACCCCTGGTTATCCACCCTTGATACCAGATGAACTCTCA CAGTCCCAATTATTATCTGCTTTGGATGGAGCAAATATGGTCTATTTTGATGGAAGACTGTACG AAACTGCTTTAATTATTGCAAAAGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.430903734

Conservation Information
Conserved circRNAs	NA
PMCS	0.430903734

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5541507-5541535(+)
5541548-5543622(-)
5541514-5543591(-)
						
						Potential amino acid sequence
						
							MSSESLPPLPEHRIVLGCGSVGVDFLASVSSYPKPDDKIRSTSLKVQGGGNAGNALTCVARLGL
NPRLISKVANDAQGKSILEELESDGVDTSFFIVSEEGNSPFTYIIVDNETKTRTCIHTPGYPPL
IPDELSQSQLLSALDGANMVYFDGRLYETALIIAKEGPNVIRIASTTS*(+)
MRCSGSGGSDSDDIWALFCNN*(-)
MTFGPSFAIIKAVSYSLPSK*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5541507-5541535(+) 5541548-5543622(-) 5541514-5543591(-)
Potential amino acid sequence	MSSESLPPLPEHRIVLGCGSVGVDFLASVSSYPKPDDKIRSTSLKVQGGGNAGNALTCVARLGL NPRLISKVANDAQGKSILEELESDGVDTSFFIVSEEGNSPFTYIIVDNETKTRTCIHTPGYPPL IPDELSQSQLLSALDGANMVYFDGRLYETALIIAKEGPNVIRIASTTS(+) MRCSGSGGSDSDDIWALFCNN(-) MTFGPSFAIIKAVSYSLPSK*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.004G057700_circ_g.1

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