Plant Circular RNA Database

Detail information of AT2G31350_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT2G31350_circ_g.2
					
						ID in PlantcircBase
						ath_circ_015865
					
						Alias
						At_ciR3384, Ath_circ_FC0210
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 13369075-13370194  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							AT2G31350						
					
						Parent gene annotation
						
							Hydroxyacylglutathione hydrolase 2, mitochondrial
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT2G31350_circ_g.1 AT2G31350_circ_g.3 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G31350.1:3
AT2G31350.3:3
AT2G31350.2:3

General Information
CircRNA Name	AT2G31350_circ_g.2
ID in PlantcircBase	ath_circ_015865
Alias	At_ciR3384, Ath_circ_FC0210
Organism	Arabidpsis thaliana
Position	chr2: 13369075-13370194 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ
Parent gene	AT2G31350
Parent gene annotation	Hydroxyacylglutathione hydrolase 2, mitochondrial
Parent gene strand	+
Alternative splicing	AT2G31350_circ_g.1 AT2G31350_circ_g.3
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G31350.1:3 AT2G31350.3:3 AT2G31350.2:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCATATCAGCTTATACTTTCCAGGATCACGAGCGATCTTCACCGGGGACACGATGTTTAGCTTA
TCATGTGGTAAACTCTTTGAAGGCACTCCTAAGCAGgtgccttgccttaaggataactatgctt
atatcttgcatgacgaggatactggtacagttggggtggttgacccttctgaagctgaacctat
tatcgatt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTGCCTTGCCTTAAGGATAACTATGCTTATATCTTGCATGACGAGGATACTGGTACAGTTGGGG
TGGTTGACCCTTCTGAAGCTGAACCTATTATCGATTCGTTGAAGAGGAGTGGTCGTAATCTAAC
ATATATATTAAATACACATCATCATTATGATCACACTGGTGGGAATTTGGAATTAAAAGACAGG
TACGGGGCAAAGGTAGGATGTTTTAACTTCTCTTTATGTACTCGTTGTGCAATGATAACATTTA
AAAAGAATGTTGGTATAAACGAAGGTTACATCTTTGGTATGCAGGTGATTGGCTCAGCTATGGA
TAAAGACCGGATTCCTGGAATTGATATGGCCTTGAAGGATGGTGACAAATGGATGTTTGCTGGC
CATGAAGTCCATGTTATGGATACTCCTGGCCACACAAAAGGTTGTTTATAATTCTCATATTAAC
TTTTGGAGCTTTGAATGCATGTTCTTGCATTCCAGTTAAGCCTTTGCTTTGCTAAATATTCCAC
TTTAAAGTAGTTCGGAATTCGATTATTTCAGCCTATGTTCAGTGTCTTCATTATATCTCAGTTA
TGTATGAATTTAAGCTGAGATTGGAGATTTGGTTTAGCTACGGTGGATTTTGTGGTCGTAACTC
TTAACTTGCCGCCGAAGATAGAATTGTATGTCTTGTTCAAAGAAGCCAAATGCACATCTAAACG
TACAGGAGTTTGACTATTTTTCAACAGACTTGTGGATTATGGGGAATTTTGTCTGGAAAAGTTG
GGGTTTCTCTCTTATGATCCTTGGGTTTTAAAGTTTGTACATGCATTGTATTGAGGAAACATGA
GAGTTCTACCATAAATTATGTAACCAGAACAGTTCTGTTCTTCTCTTCTTCAAATTAGATATGC
ATATGGCCAACATCTATCTGTGGTTTTGACGGTTTCTGCGAAAACCATCCCGTTATATACCTCT
TAAGCGTTGTTGTCCCATGTTTTAGTCTTTGTCTCTTATCTTCTTAATTATACTTGCAGGCCAT
ATCAGCTTATACTTTCCAGGATCACGAGCGATCTTCACCGGGGACACGATGTTTAGCTTATCAT
GTGGTAAACTCTTTGAAGGCACTCCTAAGCAG

Sequences
Splice junction sequence	CCATATCAGCTTATACTTTCCAGGATCACGAGCGATCTTCACCGGGGACACGATGTTTAGCTTA TCATGTGGTAAACTCTTTGAAGGCACTCCTAAGCAGgtgccttgccttaaggataactatgctt atatcttgcatgacgaggatactggtacagttggggtggttgacccttctgaagctgaacctat tatcgatt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTGCCTTGCCTTAAGGATAACTATGCTTATATCTTGCATGACGAGGATACTGGTACAGTTGGGG TGGTTGACCCTTCTGAAGCTGAACCTATTATCGATTCGTTGAAGAGGAGTGGTCGTAATCTAAC ATATATATTAAATACACATCATCATTATGATCACACTGGTGGGAATTTGGAATTAAAAGACAGG TACGGGGCAAAGGTAGGATGTTTTAACTTCTCTTTATGTACTCGTTGTGCAATGATAACATTTA AAAAGAATGTTGGTATAAACGAAGGTTACATCTTTGGTATGCAGGTGATTGGCTCAGCTATGGA TAAAGACCGGATTCCTGGAATTGATATGGCCTTGAAGGATGGTGACAAATGGATGTTTGCTGGC CATGAAGTCCATGTTATGGATACTCCTGGCCACACAAAAGGTTGTTTATAATTCTCATATTAAC TTTTGGAGCTTTGAATGCATGTTCTTGCATTCCAGTTAAGCCTTTGCTTTGCTAAATATTCCAC TTTAAAGTAGTTCGGAATTCGATTATTTCAGCCTATGTTCAGTGTCTTCATTATATCTCAGTTA TGTATGAATTTAAGCTGAGATTGGAGATTTGGTTTAGCTACGGTGGATTTTGTGGTCGTAACTC TTAACTTGCCGCCGAAGATAGAATTGTATGTCTTGTTCAAAGAAGCCAAATGCACATCTAAACG TACAGGAGTTTGACTATTTTTCAACAGACTTGTGGATTATGGGGAATTTTGTCTGGAAAAGTTG GGGTTTCTCTCTTATGATCCTTGGGTTTTAAAGTTTGTACATGCATTGTATTGAGGAAACATGA GAGTTCTACCATAAATTATGTAACCAGAACAGTTCTGTTCTTCTCTTCTTCAAATTAGATATGC ATATGGCCAACATCTATCTGTGGTTTTGACGGTTTCTGCGAAAACCATCCCGTTATATACCTCT TAAGCGTTGTTGTCCCATGTTTTAGTCTTTGTCTCTTATCTTCTTAATTATACTTGCAGGCCAT ATCAGCTTATACTTTCCAGGATCACGAGCGATCTTCACCGGGGACACGATGTTTAGCTTATCAT GTGGTAAACTCTTTGAAGGCACTCCTAAGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.134515074

Conservation Information
Conserved circRNAs	NA
PMCS	0.134515074

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13369390-13370191(+)
						
						Potential amino acid sequence
						
							MDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDTMFSLSCGKLF
EGTPKQVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHYDHTGGN
LELKDRYGAKVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAI
FTGDTMFSLSCGKLFEGTPKQVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLT
YILNTHHHYDHTGGNLELKDRYGAKVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGH
TKGHISLYFPGSRAIFTGDTMFSLSCGKLFEGTPKQVPCLKDNYAYILHDEDTGTVGVVDPSEA
EPIIDSLKRSGRNLTYILNTHHHYDHTGGNLELKDRYGAKVIGSAMDKDRIPGIDMALKDGDKW
MFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDTMFSLSCGKLFEGTPKQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13369390-13370191(+)
Potential amino acid sequence	MDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDTMFSLSCGKLF EGTPKQVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLTYILNTHHHYDHTGGN LELKDRYGAKVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGHTKGHISLYFPGSRAI FTGDTMFSLSCGKLFEGTPKQVPCLKDNYAYILHDEDTGTVGVVDPSEAEPIIDSLKRSGRNLT YILNTHHHYDHTGGNLELKDRYGAKVIGSAMDKDRIPGIDMALKDGDKWMFAGHEVHVMDTPGH TKGHISLYFPGSRAIFTGDTMFSLSCGKLFEGTPKQVPCLKDNYAYILHDEDTGTVGVVDPSEA EPIIDSLKRSGRNLTYILNTHHHYDHTGGNLELKDRYGAKVIGSAMDKDRIPGIDMALKDGDKW MFAGHEVHVMDTPGHTKGHISLYFPGSRAIFTGDTMFSLSCGKLFEGTPKQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chen et al., 2017a

circRNA

Detail information of AT2G31350_circ_g.2

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