Plant Circular RNA Database

Detail information of Csa2G263970_circ_g.5

			
						 General Information
					
						CircRNA Name
						Csa2G263970_circ_g.5
					
						ID in PlantcircBase
						csa_circ_001049
					
						Alias
						Chr2:12710995_12725140
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr2: 12710995-12725140  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Csa2G263970						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							Csa2G263970_circ_g.1 Csa2G263970_circ_g.2 Csa2G263970_circ_g.3 Csa2G263970_circ_g.4 Csa2G263970_circ_g.6 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa2G263970.1:12

General Information
CircRNA Name	Csa2G263970_circ_g.5
ID in PlantcircBase	csa_circ_001049
Alias	Chr2:12710995_12725140
Organism	Cucumis sativus
Position	chrChr2: 12710995-12725140 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2
Parent gene	Csa2G263970
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	Csa2G263970_circ_g.1 Csa2G263970_circ_g.2 Csa2G263970_circ_g.3 Csa2G263970_circ_g.4 Csa2G263970_circ_g.6
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa2G263970.1:12

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GAGGCAGTCAATTGAGTGCATACTTCTTAAAAAATCTGCATGGGACGAAGATCTCTTTGTTGCT
GTAGTTAACATGTTGACATCAGCAGCACTTTATCAGgtttgactttctagataagtctgtgggc
atatcatccttgtttgagattaatagcagtaccttggtagaacatatcccagagattgtggaaa
ctcaacag

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1399
Transcript exons: 12710995-12711196,12713742-12713858,12715773-12715858,12
715941-12716045,12716163-12716234,12717543-12717631,1271
9439-12719587,12720177-12720320,12720392-12720499,127241
35-12724245,12724844-12724948,12725030-12725140
GTTTGACTTTCTAGATAAGTCTGTGGGCATATCATCCTTGTTTGAGATTAATAGCAGTACCTTG
GTAGAACATATCCCAGAGATTGTGGAAACTCAACAGCCACTACCTGTTCCTGGAATGGAAGGAA
TGTTGATTCCTTGTAAAACACGTGGACGTGTCTTGAAAGTGGTTGGTGGAAATACTGTTCTTGT
ACGTTGGGAGTATAAATTACCAGGGATGCTCATGTTGCTCATGCGTTTGGCTCAAGGGATGTAT
TTGAATAACGATGAACAAGTTGCTATACTTGACTTGTTGAGTCGGATGATTTCCTTTAATACGG
CTGTCTGTTTTGCTTTGATGGGTGGTACACACATTTCGAATCCTGAAGTTGCAGACATAGCTCG
TCCAACAGAAAAGAATTTGTGGTTGGTTGAGGTCATTTGTGTTCTCGTGAGAAACTTATCTCCT
GATTCAAGTAATGCTGCAGTGATGGCCATAGGTCTAAATATTTTGTCAAAGATGTTGAAATGTT
TCCCTGCTATTGTCACTCCTGTGGCGCTTAAGGCCAATATCTTCAATGTTGCTGGCCATGATGC
ACTGAGTGAGTCATGGCTTCAATTTGGAAAGCTCGCGAAGATGTTGCTTATTGATGTTGAGCAC
AACGATTCTGACTCCCCACTGTCGATATCACTACTGGACTTTACCATGCAACTACTGGAGACTG
GACTAGAAAATGATGCTATTGTAGCACTTATTGCTTTCTGTCTTCAGTATATTCTAGTTAATCA
TGAGTATTGGAAGTACAAAGTTAAGCATACTCGTTGGATTGTAACTTTGAAGGTGCTTGAGATG
ATGAGGAGATGCATTCTCGTAAGCTCAGGCCCTGGAAAACTGGGGCCACTTGTCCAGAATATGC
TTCTTTTTGATTCTTCCATCCATAACACTCTTTTTCGAGTGGTTTGTACGACTAGACAAGCTCT
CGAGAAACTATATATTAGCCGTCTTTCTGAACCAAGGGAGATTGAGGGTTTAGAGAATGCCATC
TGTTCTGTCCTGGATATTTTTTTCATCATGCTTTCTGCATTTTCAAAGGATTCGTCAGCTGCAC
CTGCCGTGTTTCATCAAGCATTGATATCATTGAAAACAAAACCCATTCCTATAGCAGCAGCGGT
CATTTCTTTGTTATCTTATTTTCGAAATGTGAGTGTACAAGTCGGTGCAGCAAGGGTACTGTCT
ATGTTATTTTTTATAACAGACGATGTGCAACCCCATTTGTCTAGTGCATGCTTTAGTCTTGATG
ATGAACAAATAGCAGAGTTGAGGCAGTCAATTGAGTGCATACTTCTTAAAAAATCTGCATGGGA
CGAAGATCTCTTTGTTGCTGTAGTTAACATGTTGACATCAGCAGCACTTTATCAG
						
					

						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.						
					

			
		

Sequences
Splice junction sequence	GAGGCAGTCAATTGAGTGCATACTTCTTAAAAAATCTGCATGGGACGAAGATCTCTTTGTTGCT GTAGTTAACATGTTGACATCAGCAGCACTTTATCAGgtttgactttctagataagtctgtgggc atatcatccttgtttgagattaatagcagtaccttggtagaacatatcccagagattgtggaaa ctcaacag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1399 Transcript exons: 12710995-12711196,12713742-12713858,12715773-12715858,12 715941-12716045,12716163-12716234,12717543-12717631,1271 9439-12719587,12720177-12720320,12720392-12720499,127241 35-12724245,12724844-12724948,12725030-12725140 GTTTGACTTTCTAGATAAGTCTGTGGGCATATCATCCTTGTTTGAGATTAATAGCAGTACCTTG GTAGAACATATCCCAGAGATTGTGGAAACTCAACAGCCACTACCTGTTCCTGGAATGGAAGGAA TGTTGATTCCTTGTAAAACACGTGGACGTGTCTTGAAAGTGGTTGGTGGAAATACTGTTCTTGT ACGTTGGGAGTATAAATTACCAGGGATGCTCATGTTGCTCATGCGTTTGGCTCAAGGGATGTAT TTGAATAACGATGAACAAGTTGCTATACTTGACTTGTTGAGTCGGATGATTTCCTTTAATACGG CTGTCTGTTTTGCTTTGATGGGTGGTACACACATTTCGAATCCTGAAGTTGCAGACATAGCTCG TCCAACAGAAAAGAATTTGTGGTTGGTTGAGGTCATTTGTGTTCTCGTGAGAAACTTATCTCCT GATTCAAGTAATGCTGCAGTGATGGCCATAGGTCTAAATATTTTGTCAAAGATGTTGAAATGTT TCCCTGCTATTGTCACTCCTGTGGCGCTTAAGGCCAATATCTTCAATGTTGCTGGCCATGATGC ACTGAGTGAGTCATGGCTTCAATTTGGAAAGCTCGCGAAGATGTTGCTTATTGATGTTGAGCAC AACGATTCTGACTCCCCACTGTCGATATCACTACTGGACTTTACCATGCAACTACTGGAGACTG GACTAGAAAATGATGCTATTGTAGCACTTATTGCTTTCTGTCTTCAGTATATTCTAGTTAATCA TGAGTATTGGAAGTACAAAGTTAAGCATACTCGTTGGATTGTAACTTTGAAGGTGCTTGAGATG ATGAGGAGATGCATTCTCGTAAGCTCAGGCCCTGGAAAACTGGGGCCACTTGTCCAGAATATGC TTCTTTTTGATTCTTCCATCCATAACACTCTTTTTCGAGTGGTTTGTACGACTAGACAAGCTCT CGAGAAACTATATATTAGCCGTCTTTCTGAACCAAGGGAGATTGAGGGTTTAGAGAATGCCATC TGTTCTGTCCTGGATATTTTTTTCATCATGCTTTCTGCATTTTCAAAGGATTCGTCAGCTGCAC CTGCCGTGTTTCATCAAGCATTGATATCATTGAAAACAAAACCCATTCCTATAGCAGCAGCGGT CATTTCTTTGTTATCTTATTTTCGAAATGTGAGTGTACAAGTCGGTGCAGCAAGGGTACTGTCT ATGTTATTTTTTATAACAGACGATGTGCAACCCCATTTGTCTAGTGCATGCTTTAGTCTTGATG ATGAACAAATAGCAGAGTTGAGGCAGTCAATTGAGTGCATACTTCTTAAAAAATCTGCATGGGA CGAAGATCTCTTTGTTGCTGTAGTTAACATGTTGACATCAGCAGCACTTTATCAG
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.462616939

Conservation Information
Conserved circRNAs	NA
PMCS	0.462616939

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12711114-12710997(+)
						
						Potential amino acid sequence
						
							MEGMLIPCKTRGRVLKVVGGNTVLVRWEYKLPGMLMLLMRLAQGMYLNNDEQVAILDLLSRMIS
FNTAVCFALMGGTHISNPEVADIARPTEKNLWLVEVICVLVRNLSPDSSNAAVMAIGLNILSKM
LKCFPAIVTPVALKANIFNVAGHDALSESWLQFGKLAKMLLIDVEHNDSDSPLSISLLDFTMQL
LETGLENDAIVALIAFCLQYILVNHEYWKYKVKHTRWIVTLKVLEMMRRCILVSSGPGKLGPLV
QNMLLFDSSIHNTLFRVVCTTRQALEKLYISRLSEPREIEGLENAICSVLDIFFIMLSAFSKDS
SAAPAVFHQALISLKTKPIPIAAAVISLLSYFRNVSVQVGAARVLSMLFFITDDVQPHLSSACF
SLDDEQIAELRQSIECILLKKSAWDEDLFVAVVNMLTSAALYQV*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	12711114-12710997(+)
Potential amino acid sequence	MEGMLIPCKTRGRVLKVVGGNTVLVRWEYKLPGMLMLLMRLAQGMYLNNDEQVAILDLLSRMIS FNTAVCFALMGGTHISNPEVADIARPTEKNLWLVEVICVLVRNLSPDSSNAAVMAIGLNILSKM LKCFPAIVTPVALKANIFNVAGHDALSESWLQFGKLAKMLLIDVEHNDSDSPLSISLLDFTMQL LETGLENDAIVALIAFCLQYILVNHEYWKYKVKHTRWIVTLKVLEMMRRCILVSSGPGKLGPLV QNMLLFDSSIHNTLFRVVCTTRQALEKLYISRLSEPREIEGLENAICSVLDIFFIMLSAFSKDS SAAPAVFHQALISLKTKPIPIAAAVISLLSYFRNVSVQVGAARVLSMLFFITDDVQPHLSSACF SLDDEQIAELRQSIECILLKKSAWDEDLFVAVVNMLTSAALYQV*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019

circRNA

Detail information of Csa2G263970_circ_g.5

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