Plant Circular RNA Database

Detail information of Os03g0143800_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os03g0143800_circ_g.2
					
						ID in PlantcircBase
						osa_circ_017887
					
						Alias
						Os_ciR8691
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 2439558-2441255  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0143800						
					
						Parent gene annotation
						
							PIROGI/Specifically Rac1-associated protein 1 (PIR/SRA1)-like pr
otein, Homolog of SCAR/WAVE complex components, Control of epide
rmal cell morphogenesis (Os03t0143800-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0143800_circ_g.1 Os03g0143800_circ_g.3 Os03g0143800_circ_g.4 Os03g0143800_circ_g.5 Os03g0143800_circ_g.6 Os03g0143800_circ_g.7 Os03g0143800_circ_g.8 Os03g0143800_circ_g.9 Os03g0143800_circ_g.10 Os03g0143800_circ_g.11 Os03g0143800_circ_g.12 Os03g0143800_circ_g.13 						
					
						Support reads
						2/2
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0143800-01:3

General Information
CircRNA Name	Os03g0143800_circ_g.2
ID in PlantcircBase	osa_circ_017887
Alias	Os_ciR8691
Organism	Oryza sativa
Position	chr3: 2439558-2441255 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0143800
Parent gene annotation	PIROGI/Specifically Rac1-associated protein 1 (PIR/SRA1)-like pr otein, Homolog of SCAR/WAVE complex components, Control of epide rmal cell morphogenesis (Os03t0143800-01)
Parent gene strand	+
Alternative splicing	Os03g0143800_circ_g.1 Os03g0143800_circ_g.3 Os03g0143800_circ_g.4 Os03g0143800_circ_g.5 Os03g0143800_circ_g.6 Os03g0143800_circ_g.7 Os03g0143800_circ_g.8 Os03g0143800_circ_g.9 Os03g0143800_circ_g.10 Os03g0143800_circ_g.11 Os03g0143800_circ_g.12 Os03g0143800_circ_g.13
Support reads	2/2
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0143800-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CAACACTAGCAAGGGAGATCCAGAACACAACTCGTTAGAAACAGAGGAAATGAGACAAAGCACA
TTCTACCCAAGGCCAGTTGCACCAACAGCTGCACAGatgataatattgaagtcatcagatggtg
cggataatgactggtcaagagatattaaggggaacatgtatgatattgttgttgaaggctttca
gcttctta

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 567
Transcript exons: 2439558-2439770,2440654-2440865,2441114-2441255
ATGATAATATTGAAGTCATCAGATGGTGCGGATAATGACTGGTCAAGAGATATTAAGGGGAACA
TGTATGATATTGTTGTTGAAGGCTTTCAGCTTCTTAGCCGATGGACTGGCAGGATATGGGAGCA
GTGTGCCTGGAAATTTTCGCGCCCTTGTAAAGAACCACCTTCAGATTCTCAACATGGATCGACA
ACATTTTTTGACTATGAAAAGGTAGTACGCTGGAACTACACTGGAGAAGAACGGAGGGCTCTTC
TTGAATTAATTGGTTACATAAAAAGTATTGGATTGATGATGCAACGCTGTGACACCTTAGTGTC
TGAAGCCTTATGGGAAACGATACACATGGAGGTCCAAGATTTTGTACAGGATAAACTAGATACT
ATGCTCCGTACAACATTTCGGAAGAAGAAGGACCTATCTAGGATCCTCTCTGATATGCGAACCC
TCTCCGCAGATTGGATGGCCAACACTAGCAAGGGAGATCCAGAACACAACTCGTTAGAAACAGA
GGAAATGAGACAAAGCACATTCTACCCAAGGCCAGTTGCACCAACAGCTGCACAG
						
					

						Genomic sequence
						
							ATGATAATATTGAAGTCATCAGATGGTGCGGATAATGACTGGTCAAGAGATATTAAGGGGAACA
TGTATGATATTGTTGTTGAAGGCTTTCAGCTTCTTAGCCGATGGACTGGCAGGATATGGGAGCA
GTGTGCCTGGAAATTTTCGCGCCCTTGTAAAGAACCACCTTCAGATTCTCAACATGGATCGACA
ACATTTTTTGACTATGAAAAGGTCTTCTTTCTTTTAAAGCAGTGCTGGCAGCCAATTATTTTAG
TGTCTTAGTTCTTCTATTTTGTCCTGGTTACAAACTTTGTAATTCTGTGTTTTGTTTCCTCTCC
TTTGTAAATATTCTTTTGATAATATAATATATCTACTAGATAAGGCCGGTAGTATTTTCAGTAA
AAAAATATTTTTCTTCTACTCCATTTCCTTATGTTTGAAATTGTTTGTGCATTGTTTATTGAAA
ACCTTGCATCCCTTTTCTTTTCTTTACCCAGAATGATAATTGTACTAATGCTTAATTCCAATCA
ATATAGGCCTCAGAATTCTAAGATACATTTCAAACATCAAATTCATGAATATAATGATTGGAAA
TTTAGAATTCCTTTGATCCTTTACTATGTCGGAAACTTTAGTCTGAATTTATACACATGCATGA
TCATCAATCTCCAGGGATGGGTTCGCAAGGTTTCTTGTTATTTTTTTTCCCATTACGGGATAGA
TTAAATACAGAACCCTGATTTGGCTTGTATACACTAAGTGAGGCACATGTGGGCACAGGAAGAG
ACCTTTACACCTGATTTGTTGGCTGAAACTAATTGATTGAATCTTTCTGTTCATATTTCTACAT
TCTTCCACCCTGACTAGGATAATGGTGCCTACCTGTTGCATACTCGCATTGTGTAGTGGGCGTG
TAGCCTTGAAATGTCAGTAAATTAGTTCCTTTCTCTAGACCTAAAAAAATAACACATCCCAAAA
ATGTATTATTTTATTTTTAATATTTTTCTTCCTTTTTGTTTTGATATAGGTTTTTTTAATGTTT
ACAACTCAATTTTATTGGTTATGGACATTGGACATCTTGATTGATTATGGACATTTAACAACTT
ACATGCAGGTAGTACGCTGGAACTACACTGGAGAAGAACGGAGGGCTCTTCTTGAATTAATTGG
TTACATAAAAAGTATTGGATTGATGATGCAACGCTGTGACACCTTAGTGTCTGAAGCCTTATGG
GAAACGATACACATGGAGGTCCAAGATTTTGTACAGGATAAACTAGATACTATGCTCCGTACAA
CATTTCGGAAGAAGAAGGACCTATCTAGGTTACTTGTTCGTCTCAACCATCCTTATTTTAACAT
GATACTGTAAATTTGCCACCAGCAGTTCCAATAGGCTCCAATAAATCTTGTTCTATGTTTAGTT
CAATCTGGGGATGACATAGTTACTTATGTCAATTCTGTATTTGTCATTTATATTTATTTATGTA
TGATGCCAATTCTTCCTGAAATTTGAGAATTCATTTGGGAACTTAAATTTAAGTTTGTCCATTT
TTCCTTTGCTTTTCTTTCAGGATCCTCTCTGATATGCGAACCCTCTCCGCAGATTGGATGGCCA
ACACTAGCAAGGGAGATCCAGAACACAACTCGTTAGAAACAGAGGAAATGAGACAAAGCACATT
CTACCCAAGGCCAGTTGCACCAACAGCTGCACAG
						
					

			
		

Sequences
Splice junction sequence	CAACACTAGCAAGGGAGATCCAGAACACAACTCGTTAGAAACAGAGGAAATGAGACAAAGCACA TTCTACCCAAGGCCAGTTGCACCAACAGCTGCACAGatgataatattgaagtcatcagatggtg cggataatgactggtcaagagatattaaggggaacatgtatgatattgttgttgaaggctttca gcttctta
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 567 Transcript exons: 2439558-2439770,2440654-2440865,2441114-2441255 ATGATAATATTGAAGTCATCAGATGGTGCGGATAATGACTGGTCAAGAGATATTAAGGGGAACA TGTATGATATTGTTGTTGAAGGCTTTCAGCTTCTTAGCCGATGGACTGGCAGGATATGGGAGCA GTGTGCCTGGAAATTTTCGCGCCCTTGTAAAGAACCACCTTCAGATTCTCAACATGGATCGACA ACATTTTTTGACTATGAAAAGGTAGTACGCTGGAACTACACTGGAGAAGAACGGAGGGCTCTTC TTGAATTAATTGGTTACATAAAAAGTATTGGATTGATGATGCAACGCTGTGACACCTTAGTGTC TGAAGCCTTATGGGAAACGATACACATGGAGGTCCAAGATTTTGTACAGGATAAACTAGATACT ATGCTCCGTACAACATTTCGGAAGAAGAAGGACCTATCTAGGATCCTCTCTGATATGCGAACCC TCTCCGCAGATTGGATGGCCAACACTAGCAAGGGAGATCCAGAACACAACTCGTTAGAAACAGA GGAAATGAGACAAAGCACATTCTACCCAAGGCCAGTTGCACCAACAGCTGCACAG
Genomic sequence	ATGATAATATTGAAGTCATCAGATGGTGCGGATAATGACTGGTCAAGAGATATTAAGGGGAACA TGTATGATATTGTTGTTGAAGGCTTTCAGCTTCTTAGCCGATGGACTGGCAGGATATGGGAGCA GTGTGCCTGGAAATTTTCGCGCCCTTGTAAAGAACCACCTTCAGATTCTCAACATGGATCGACA ACATTTTTTGACTATGAAAAGGTCTTCTTTCTTTTAAAGCAGTGCTGGCAGCCAATTATTTTAG TGTCTTAGTTCTTCTATTTTGTCCTGGTTACAAACTTTGTAATTCTGTGTTTTGTTTCCTCTCC TTTGTAAATATTCTTTTGATAATATAATATATCTACTAGATAAGGCCGGTAGTATTTTCAGTAA AAAAATATTTTTCTTCTACTCCATTTCCTTATGTTTGAAATTGTTTGTGCATTGTTTATTGAAA ACCTTGCATCCCTTTTCTTTTCTTTACCCAGAATGATAATTGTACTAATGCTTAATTCCAATCA ATATAGGCCTCAGAATTCTAAGATACATTTCAAACATCAAATTCATGAATATAATGATTGGAAA TTTAGAATTCCTTTGATCCTTTACTATGTCGGAAACTTTAGTCTGAATTTATACACATGCATGA TCATCAATCTCCAGGGATGGGTTCGCAAGGTTTCTTGTTATTTTTTTTCCCATTACGGGATAGA TTAAATACAGAACCCTGATTTGGCTTGTATACACTAAGTGAGGCACATGTGGGCACAGGAAGAG ACCTTTACACCTGATTTGTTGGCTGAAACTAATTGATTGAATCTTTCTGTTCATATTTCTACAT TCTTCCACCCTGACTAGGATAATGGTGCCTACCTGTTGCATACTCGCATTGTGTAGTGGGCGTG TAGCCTTGAAATGTCAGTAAATTAGTTCCTTTCTCTAGACCTAAAAAAATAACACATCCCAAAA ATGTATTATTTTATTTTTAATATTTTTCTTCCTTTTTGTTTTGATATAGGTTTTTTTAATGTTT ACAACTCAATTTTATTGGTTATGGACATTGGACATCTTGATTGATTATGGACATTTAACAACTT ACATGCAGGTAGTACGCTGGAACTACACTGGAGAAGAACGGAGGGCTCTTCTTGAATTAATTGG TTACATAAAAAGTATTGGATTGATGATGCAACGCTGTGACACCTTAGTGTCTGAAGCCTTATGG GAAACGATACACATGGAGGTCCAAGATTTTGTACAGGATAAACTAGATACTATGCTCCGTACAA CATTTCGGAAGAAGAAGGACCTATCTAGGTTACTTGTTCGTCTCAACCATCCTTATTTTAACAT GATACTGTAAATTTGCCACCAGCAGTTCCAATAGGCTCCAATAAATCTTGTTCTATGTTTAGTT CAATCTGGGGATGACATAGTTACTTATGTCAATTCTGTATTTGTCATTTATATTTATTTATGTA TGATGCCAATTCTTCCTGAAATTTGAGAATTCATTTGGGAACTTAAATTTAAGTTTGTCCATTT TTCCTTTGCTTTTCTTTCAGGATCCTCTCTGATATGCGAACCCTCTCCGCAGATTGGATGGCCA ACACTAGCAAGGGAGATCCAGAACACAACTCGTTAGAAACAGAGGAAATGAGACAAAGCACATT CTACCCAAGGCCAGTTGCACCAACAGCTGCACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_013354 						
					
						PMCS
						0.343330948

Conservation Information
Conserved circRNAs	osi_circ_013354
PMCS	0.343330948

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2439559-2441252(+)
2439624-2441224(-)
2439578-2441163(-)
						
						Potential amino acid sequence
						
							MIILKSSDGADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGST
TFFDYEKVVRWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDT
MLRTTFRKKKDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQMII
LKSSDGADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGSTTFF
DYEKVVRWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDTMLR
TTFRKKKDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQMIILKS
SDGADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGSTTFFDYE
KVVRWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDTMLRTTF
RKKKDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQMIILKSSDG
ADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGSTTFFDYEKVV
RWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDTMLRTTFRKK
KDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQ(+)
MFPLISLDQSLSAPSDDFNIIICAAVGATGLG*(-)
MTSILSSVQLLVQLALGRMCFVSFPLFLTSCVLDLPC*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2439559-2441252(+) 2439624-2441224(-) 2439578-2441163(-)
Potential amino acid sequence	MIILKSSDGADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGST TFFDYEKVVRWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDT MLRTTFRKKKDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQMII LKSSDGADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGSTTFF DYEKVVRWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDTMLR TTFRKKKDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQMIILKS SDGADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGSTTFFDYE KVVRWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDTMLRTTF RKKKDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQMIILKSSDG ADNDWSRDIKGNMYDIVVEGFQLLSRWTGRIWEQCAWKFSRPCKEPPSDSQHGSTTFFDYEKVV RWNYTGEERRALLELIGYIKSIGLMMQRCDTLVSEALWETIHMEVQDFVQDKLDTMLRTTFRKK KDLSRILSDMRTLSADWMANTSKGDPEHNSLETEEMRQSTFYPRPVAPTAAQ(+) MFPLISLDQSLSAPSDDFNIIICAAVGATGLG(-) MTSILSSVQLLVQLALGRMCFVSFPLFLTSCVLDLPC(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0143800_circ_g.2

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