Plant Circular RNA Database

Detail information of Os05g0567100_circ_g.12

			
						 General Information
					
						CircRNA Name
						Os05g0567100_circ_g.12
					
						ID in PlantcircBase
						osa_circ_029007
					
						Alias
						Os05circ18841
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 28236253-28236612  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, SMALT, Segemehl, circseq_cup
					
						Parent gene
						
							Os05g0567100						
					
						Parent gene annotation
						
							Aspartic proteinase oryzasin 1 precursor (EC 3.4.23.-). (Os05t05
67100-01);Aspartic proteinase oryzasin 1 precursor (EC 3.4.23.-)
. (Os05t0567100-02);Similar to Phytepsin. (Os05t0567100-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0567100_circ_g.2 Os05g0567100_circ_g.3 Os05g0567100_circ_g.4 Os05g0567100_circ_g.5 Os05g0567100_circ_g.6 Os05g0567100_circ_g.7 Os05g0567100_circ_g.8 Os05g0567100_circ_g.9 Os05g0567100_circ_g.10 Os05g0567100_circ_g.11 Os05g0567100_circ_g.13 						
					
						Support reads
						2/3/5
					
						Tissues
						leaf/root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0567100-01:2
Os05t0567100-02:2
Os05t0567100-03:2

General Information
CircRNA Name	Os05g0567100_circ_g.12
ID in PlantcircBase	osa_circ_029007
Alias	Os05circ18841
Organism	Oryza sativa
Position	chr5: 28236253-28236612 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, SMALT, Segemehl, circseq_cup
Parent gene	Os05g0567100
Parent gene annotation	Aspartic proteinase oryzasin 1 precursor (EC 3.4.23.-). (Os05t05 67100-01);Aspartic proteinase oryzasin 1 precursor (EC 3.4.23.-) . (Os05t0567100-02);Similar to Phytepsin. (Os05t0567100-03)
Parent gene strand	+
Alternative splicing	Os05g0567100_circ_g.2 Os05g0567100_circ_g.3 Os05g0567100_circ_g.4 Os05g0567100_circ_g.5 Os05g0567100_circ_g.6 Os05g0567100_circ_g.7 Os05g0567100_circ_g.8 Os05g0567100_circ_g.9 Os05g0567100_circ_g.10 Os05g0567100_circ_g.11 Os05g0567100_circ_g.13
Support reads	2/3/5
Tissues	leaf/root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0567100-01:2 Os05t0567100-02:2 Os05t0567100-03:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCCAATGGGAGAATCATCTGTGGACTGTGGCAGCCTTGCATCCATGCCTGAGATTTCATTCACC
ATTGGAGGCAAAAAGTTTGCACTGAAGCCAGAAGAGtgctggaattaagagcgtagtagatgat
gaagctggggaatcgaatggtctccaaagtggtcccatgtgcaatgcctgtgagatggctgttg
tatggatg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 277
Transcript exons: 28236253-28236409,28236493-28236612
TGCTGGAATTAAGAGCGTAGTAGATGATGAAGCTGGGGAATCGAATGGTCTCCAAAGTGGTCCC
ATGTGCAATGCCTGTGAGATGGCTGTTGTATGGATGCAGAACCAACTTGCACAGAACAAGACTC
AGGATCTCATCTTGAACTACATTAATCAGCTCTGTGACAAGCTCCCAAGTCCAATGGGAGAATC
ATCTGTGGACTGTGGCAGCCTTGCATCCATGCCTGAGATTTCATTCACCATTGGAGGCAAAAAG
TTTGCACTGAAGCCAGAAGAG
						
						Genomic sequence
						
							TGCTGGAATTAAGAGCGTAGTAGATGATGAAGCTGGGGAATCGAATGGTCTCCAAAGTGGTCCC
ATGTGCAATGCCTGTGAGATGGCTGTTGTATGGATGCAGAACCAACTTGCACAGAACAAGACTC
AGGATCTCATCTTGAACTACATTAATCAGGTGAGCTTAGTTAACTCCTGCTCTAGGGCCATTGT
ACTGGTAGGGATAGATTTTCCGTTGCTTAACTGTTATCAATTTTACAGCTCTGTGACAAGCTCC
CAAGTCCAATGGGAGAATCATCTGTGGACTGTGGCAGCCTTGCATCCATGCCTGAGATTTCATT
CACCATTGGAGGCAAAAAGTTTGCACTGAAGCCAGAAGAG

Sequences
Splice junction sequence	TCCAATGGGAGAATCATCTGTGGACTGTGGCAGCCTTGCATCCATGCCTGAGATTTCATTCACC ATTGGAGGCAAAAAGTTTGCACTGAAGCCAGAAGAGtgctggaattaagagcgtagtagatgat gaagctggggaatcgaatggtctccaaagtggtcccatgtgcaatgcctgtgagatggctgttg tatggatg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 277 Transcript exons: 28236253-28236409,28236493-28236612 TGCTGGAATTAAGAGCGTAGTAGATGATGAAGCTGGGGAATCGAATGGTCTCCAAAGTGGTCCC ATGTGCAATGCCTGTGAGATGGCTGTTGTATGGATGCAGAACCAACTTGCACAGAACAAGACTC AGGATCTCATCTTGAACTACATTAATCAGCTCTGTGACAAGCTCCCAAGTCCAATGGGAGAATC ATCTGTGGACTGTGGCAGCCTTGCATCCATGCCTGAGATTTCATTCACCATTGGAGGCAAAAAG TTTGCACTGAAGCCAGAAGAG
Genomic sequence	TGCTGGAATTAAGAGCGTAGTAGATGATGAAGCTGGGGAATCGAATGGTCTCCAAAGTGGTCCC ATGTGCAATGCCTGTGAGATGGCTGTTGTATGGATGCAGAACCAACTTGCACAGAACAAGACTC AGGATCTCATCTTGAACTACATTAATCAGGTGAGCTTAGTTAACTCCTGCTCTAGGGCCATTGT ACTGGTAGGGATAGATTTTCCGTTGCTTAACTGTTATCAATTTTACAGCTCTGTGACAAGCTCC CAAGTCCAATGGGAGAATCATCTGTGGACTGTGGCAGCCTTGCATCCATGCCTGAGATTTCATT CACCATTGGAGGCAAAAAGTTTGCACTGAAGCCAGAAGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_015783 						
					
						PMCS
						0.44904588

Conservation Information
Conserved circRNAs	osi_circ_015783
PMCS	0.44904588

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							28236318-28236261(+)
28236320-28236570(-)
						
						Potential amino acid sequence
						
							MCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCGSLASMPEISFTIGGKK
FALKPEECWN*(+)
MGPLWRPFDSPASSSTTLLIPALFWLQCKLFASNGE*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	28236318-28236261(+) 28236320-28236570(-)
Potential amino acid sequence	MCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPMGESSVDCGSLASMPEISFTIGGKK FALKPEECWN(+) MGPLWRPFDSPASSSTTLLIPALFWLQCKLFASNGE(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Ye et al., 2016;Chu et al., 2017

circRNA

Detail information of Os05g0567100_circ_g.12

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