Plant Circular RNA Database

Detail information of AT5G04560_circ_g.6

			
						 General Information
					
						CircRNA Name
						AT5G04560_circ_g.6
					
						ID in PlantcircBase
						ath_circ_036420
					
						Alias
						Ath_circ_FC3150
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 1315088-1315825  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							AT5G04560						
					
						Parent gene annotation
						
							Transcriptional activator DEMETER
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G04560_circ_g.3 AT5G04560_circ_g.4 AT5G04560_circ_g.5 AT5G04560_circ_g.7 						
					
						Support reads
						1
					
						Tissues
						aerial, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G04560.3:5
AT5G04560.1:5
AT5G04560.2:5

General Information
CircRNA Name	AT5G04560_circ_g.6
ID in PlantcircBase	ath_circ_036420
Alias	Ath_circ_FC3150
Organism	Arabidpsis thaliana
Position	chr5: 1315088-1315825 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	AT5G04560
Parent gene annotation	Transcriptional activator DEMETER
Parent gene strand	+
Alternative splicing	AT5G04560_circ_g.3 AT5G04560_circ_g.4 AT5G04560_circ_g.5 AT5G04560_circ_g.7
Support reads	1
Tissues	aerial, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G04560.3:5 AT5G04560.1:5 AT5G04560.2:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATGTGAATTCATGTCAGGTATTTTGCACAAAGAGTAGACCAAATTGTAATGCATGTCCAATGAG
AGGAGAGTGCAGACACTTTGCCAGTGCTTATGCTAGggactatctcttgagcataagaggtctg
ggtttgaaaagtgttgaatgcgtgcgactcttaacactccacaatcttgctttccctgtgagtc
agactatt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 366
Transcript exons: 1315088-1315172,1315256-1315347,1315421-1315489,1315609-
1315645,1315743-1315825
GGACTATCTCTTGAGCATAAGAGGTCTGGGTTTGAAAAGTGTTGAATGCGTGCGACTCTTAACA
CTCCACAATCTTGCTTTCCCTGTTGACACGAATGTTGGAAGGATAGCAGTTAGGATGGGATGGG
TGCCTCTACAACCCCTACCTGAATCACTTCAGTTACACCTCCTGGAGCTATACCCAGTGCTCGA
GTCCATCCAAAAATTTCTTTGGCCAAGACTTTGCAAACTCGATCAACGAACACTGTATGAATTA
CACTACCAACTGATTACGTTTGGAAAGGTATTTTGCACAAAGAGTAGACCAAATTGTAATGCAT
GTCCAATGAGAGGAGAGTGCAGACACTTTGCCAGTGCTTATGCTAG
						
						Genomic sequence
						
							GGACTATCTCTTGAGCATAAGAGGTCTGGGTTTGAAAAGTGTTGAATGCGTGCGACTCTTAACA
CTCCACAATCTTGCTTTCCCTGTGAGTCAGACTATTCCATTATCTACTAAAAACTTAGAATAAC
TCCGGCTAACTAAGCTGGAACTTGTATTGATGATATGAAGGTTGACACGAATGTTGGAAGGATA
GCAGTTAGGATGGGATGGGTGCCTCTACAACCCCTACCTGAATCACTTCAGTTACACCTCCTGG
AGCTGTAAGTTTCTTTTTGTTTGTCATCTAAACAACGAAATTTTTATGCAAGTCATAACCATGC
TGTGTTTTCACAGATACCCAGTGCTCGAGTCCATCCAAAAATTTCTTTGGCCAAGACTTTGCAA
ACTCGATCAACGAACACTGTATGCTCATAAACTCTAACAAATCATCTGTCTGAAAAACCAATAT
TTCTTTGGTAGAATTCTATTGTCATTACTCATTACTAACAGCGAAATTAATTAACGTTCTTTTT
CTTACTCAGGTATGAATTACACTACCAACTGATTACGTTTGGAAAGGTATTATTGCTCTAAGCT
TTGAATTTATCATATGGTAATTTCAAGCATTGTAGGCACCTGATCAATTATGTGTCTAAATCAT
GTGAATTCATGTCAGGTATTTTGCACAAAGAGTAGACCAAATTGTAATGCATGTCCAATGAGAG
GAGAGTGCAGACACTTTGCCAGTGCTTATGCTAG

Sequences
Splice junction sequence	ATGTGAATTCATGTCAGGTATTTTGCACAAAGAGTAGACCAAATTGTAATGCATGTCCAATGAG AGGAGAGTGCAGACACTTTGCCAGTGCTTATGCTAGggactatctcttgagcataagaggtctg ggtttgaaaagtgttgaatgcgtgcgactcttaacactccacaatcttgctttccctgtgagtc agactatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 366 Transcript exons: 1315088-1315172,1315256-1315347,1315421-1315489,1315609- 1315645,1315743-1315825 GGACTATCTCTTGAGCATAAGAGGTCTGGGTTTGAAAAGTGTTGAATGCGTGCGACTCTTAACA CTCCACAATCTTGCTTTCCCTGTTGACACGAATGTTGGAAGGATAGCAGTTAGGATGGGATGGG TGCCTCTACAACCCCTACCTGAATCACTTCAGTTACACCTCCTGGAGCTATACCCAGTGCTCGA GTCCATCCAAAAATTTCTTTGGCCAAGACTTTGCAAACTCGATCAACGAACACTGTATGAATTA CACTACCAACTGATTACGTTTGGAAAGGTATTTTGCACAAAGAGTAGACCAAATTGTAATGCAT GTCCAATGAGAGGAGAGTGCAGACACTTTGCCAGTGCTTATGCTAG
Genomic sequence	GGACTATCTCTTGAGCATAAGAGGTCTGGGTTTGAAAAGTGTTGAATGCGTGCGACTCTTAACA CTCCACAATCTTGCTTTCCCTGTGAGTCAGACTATTCCATTATCTACTAAAAACTTAGAATAAC TCCGGCTAACTAAGCTGGAACTTGTATTGATGATATGAAGGTTGACACGAATGTTGGAAGGATA GCAGTTAGGATGGGATGGGTGCCTCTACAACCCCTACCTGAATCACTTCAGTTACACCTCCTGG AGCTGTAAGTTTCTTTTTGTTTGTCATCTAAACAACGAAATTTTTATGCAAGTCATAACCATGC TGTGTTTTCACAGATACCCAGTGCTCGAGTCCATCCAAAAATTTCTTTGGCCAAGACTTTGCAA ACTCGATCAACGAACACTGTATGCTCATAAACTCTAACAAATCATCTGTCTGAAAAACCAATAT TTCTTTGGTAGAATTCTATTGTCATTACTCATTACTAACAGCGAAATTAATTAACGTTCTTTTT CTTACTCAGGTATGAATTACACTACCAACTGATTACGTTTGGAAAGGTATTATTGCTCTAAGCT TTGAATTTATCATATGGTAATTTCAAGCATTGTAGGCACCTGATCAATTATGTGTCTAAATCAT GTGAATTCATGTCAGGTATTTTGCACAAAGAGTAGACCAAATTGTAATGCATGTCCAATGAGAG GAGAGTGCAGACACTTTGCCAGTGCTTATGCTAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.272143772

Conservation Information
Conserved circRNAs	NA
PMCS	0.272143772

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1315289-1315822(+)
						
						Potential amino acid sequence
						
							MGWVPLQPLPESLQLHLLELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPN
CNACPMRGECRHFASAYARDYLLSIRGLGLKSVECVRLLTLHNLAFPVDTNVGRIAVRMGWVPL
QPLPESLQLHLLELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPNCNACPM
RGECRHFASAYARDYLLSIRGLGLKSVECVRLLTLHNLAFPVDTNVGRIAVRMGWVPLQPLPES
LQLHLLELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPNCNACPMRGECRH
FASAYARDYLLSIRGLGLKSVECVRLLTLHNLAFPVDTNVGRIAVRMGWVPLQPLPESLQLHLL
ELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPNCNACPMRGECRHFASAYA
(+)
						
						Sponge-miRNAs
						
							ath-miR827 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1315289-1315822(+)
Potential amino acid sequence	MGWVPLQPLPESLQLHLLELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPN CNACPMRGECRHFASAYARDYLLSIRGLGLKSVECVRLLTLHNLAFPVDTNVGRIAVRMGWVPL QPLPESLQLHLLELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPNCNACPM RGECRHFASAYARDYLLSIRGLGLKSVECVRLLTLHNLAFPVDTNVGRIAVRMGWVPLQPLPES LQLHLLELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPNCNACPMRGECRH FASAYARDYLLSIRGLGLKSVECVRLLTLHNLAFPVDTNVGRIAVRMGWVPLQPLPESLQLHLL ELYPVLESIQKFLWPRLCKLDQRTLYELHYQLITFGKVFCTKSRPNCNACPMRGECRHFASAYA (+)
Sponge-miRNAs	ath-miR827
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT5G04560_circ_g.6

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