Plant Circular RNA Database

Detail information of orai.002G146100_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.002G146100_circ_g.2
					
						ID in PlantcircBase
						gra_circ_000163
					
						Alias
						Chr02:26793522|26798789
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr02: 26793522-26798789  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.002G146100						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							orai.002G146100_circ_g.1 						
					
						Support reads
						2
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.002G146100.2:4
Gorai.002G146100.4:4
Gorai.002G146100.5:4
Gorai.002G146100.6:3
Gorai.002G146100.3:4
Gorai.002G146100.1:4

General Information
CircRNA Name	orai.002G146100_circ_g.2
ID in PlantcircBase	gra_circ_000163
Alias	Chr02:26793522\|26798789
Organism	Gossypium raimondii
Position	chrChr02: 26793522-26798789 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.002G146100
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	orai.002G146100_circ_g.1
Support reads	2
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.002G146100.2:4 Gorai.002G146100.4:4 Gorai.002G146100.5:4 Gorai.002G146100.6:3 Gorai.002G146100.3:4 Gorai.002G146100.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATGAATCTGATGAGTTCAATTTTTGGTTCTTATACAGGGCAGCAGACTGCTCTAGAGAACTAGA
CCTTGAATCTGGGACAACATCCTGAGATTGCTCATTcttatctgctttctgaatatttccttca
tgttcaggattcaaacatgaatttgaaaaattactagtatcttgtccttgcacttcgcatccat
taattctc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 840
Transcript exons: 26793522-26793716,26794343-26794558,26797041-26797256,26
798577-26798789
CTTATCTGCTTTCTGAATATTTCCTTCATGTTCAGGATTCAAACATGAATTTGAAAAATTACTA
GTATCTTGTCCTTGCACTTCGCATCCATTAATTCTCACGTCATGCTTGCCAAGCGTTTTCATCT
CATTGGAGTCGCCAGTCATCTGTTTATGAGCAATCAAGCCCAAATCGTTGGCAATAATATCTGC
AGTCTTCACTTTCTTTGTAAGATCCCCTGTCATCCCCATTGAATTTGATGATGAATCTAGGATT
AACATATCTGCAGCAGATATAGAAGTGTGCTTTGTTGCTTGCTCACTCAAAATTTTCAAACTAG
AAGAACTTGTTTTTTTCTTCTTTTTGAGGCGGCCAGCTGAATGTTTTGAAGCATCCAGATCTGA
GTCATTCACACACTGGGCAGCTGCATCCTTAACCTTAGTCCTGTATGGATTATTCTTTCTCTTC
TGAGCAGACTTGAGAGAAAAATGCCGGACCACATTTTTATCCTTAAGAGCACAACCACTTTCTT
TGAGCTTCTTGGTAGTTTCTAAATCAAGTAAAGTTATAATGCTCTTCTCATCAAATTGACCAGC
TGCTGGCAGCAGGAAATGTGGCATCAGATCTTTAACATCGGCATTGGCTAGTGTTTCTGAACTA
TTTGCCTTCTTCACTTCAAGCTCCATAGATCGATTTGATGAAGTAAAATTTTCTTTGGGAGTAA
CATTGGGTCCATTGTTTTCCTCCTTTGCAATGAAACATGAATCTGATGAGTTCAATTTTTGGTT
CTTATACAGGGCAGCAGACTGCTCTAGAGAACTAGACCTTGAATCTGGGACAACATCCTGAGAT
TGCTCATT
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	ATGAATCTGATGAGTTCAATTTTTGGTTCTTATACAGGGCAGCAGACTGCTCTAGAGAACTAGA CCTTGAATCTGGGACAACATCCTGAGATTGCTCATTcttatctgctttctgaatatttccttca tgttcaggattcaaacatgaatttgaaaaattactagtatcttgtccttgcacttcgcatccat taattctc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 840 Transcript exons: 26793522-26793716,26794343-26794558,26797041-26797256,26 798577-26798789 CTTATCTGCTTTCTGAATATTTCCTTCATGTTCAGGATTCAAACATGAATTTGAAAAATTACTA GTATCTTGTCCTTGCACTTCGCATCCATTAATTCTCACGTCATGCTTGCCAAGCGTTTTCATCT CATTGGAGTCGCCAGTCATCTGTTTATGAGCAATCAAGCCCAAATCGTTGGCAATAATATCTGC AGTCTTCACTTTCTTTGTAAGATCCCCTGTCATCCCCATTGAATTTGATGATGAATCTAGGATT AACATATCTGCAGCAGATATAGAAGTGTGCTTTGTTGCTTGCTCACTCAAAATTTTCAAACTAG AAGAACTTGTTTTTTTCTTCTTTTTGAGGCGGCCAGCTGAATGTTTTGAAGCATCCAGATCTGA GTCATTCACACACTGGGCAGCTGCATCCTTAACCTTAGTCCTGTATGGATTATTCTTTCTCTTC TGAGCAGACTTGAGAGAAAAATGCCGGACCACATTTTTATCCTTAAGAGCACAACCACTTTCTT TGAGCTTCTTGGTAGTTTCTAAATCAAGTAAAGTTATAATGCTCTTCTCATCAAATTGACCAGC TGCTGGCAGCAGGAAATGTGGCATCAGATCTTTAACATCGGCATTGGCTAGTGTTTCTGAACTA TTTGCCTTCTTCACTTCAAGCTCCATAGATCGATTTGATGAAGTAAAATTTTCTTTGGGAGTAA CATTGGGTCCATTGTTTTCCTCCTTTGCAATGAAACATGAATCTGATGAGTTCAATTTTTGGTT CTTATACAGGGCAGCAGACTGCTCTAGAGAACTAGACCTTGAATCTGGGACAACATCCTGAGAT TGCTCATT
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.755623655

Conservation Information
Conserved circRNAs	NA
PMCS	0.755623655

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26798616-26793524(-)
26793551-26798743(-)
						
						Potential amino acid sequence
						
							MELEVKKANSSETLANADVKDLMPHFLLPAAGQFDEKSIITLLDLETTKKLKESGCALKDKNVV
RHFSLKSAQKRKNNPYRTKVKDAAAQCVNDSDLDASKHSAGRLKKKKKTSSSSLKILSEQATKH
TSISAADMLILDSSSNSMGMTGDLTKKVKTADIIANDLGLIAHKQMTGDSNEMKTLGKHDVRIN
GCEVQGQDTSNFSNSCLNPEHEGNIQKADKNEQSQDVVPDSRSSSLEQSAALYKNQKLNSSDSC
FIAKEENNGPNVTPKENFTSSNRSMELEVKKANSSETLANADVKDLMPHFLLPAAGQFDEKSII
TLLDLETTKKLKESGCALKDKNVVRHFSLKSAQKRKNNPYRTKVKDAAAQCVNDSDLDASKHSA
GRLKKKKKTSSSSLKILSEQATKHTSISAADMLILDSSSNSMGMTGDLTKKVKTADIIANDLGL
IAHKQMTGDSNEMKTLGKHDVRINGCEVQGQDTSNFSNSCLNPEHEGNIQKADKNEQSQDVVPD
SRSSSLEQSAALYKNQKLNSSDSCFIAKEENNGPNVTPKENFTSSNRSMELEVKKANSSETLAN
ADVKDLMPHFLLPAAGQFDEKSIITLLDLETTKKLKESGCALKDKNVVRHFSLKSAQKRKNNPY
RTKVKDAAAQCVNDSDLDASKHSAGRLKKKKKTSSSSLKILSEQATKHTSISAADMLILDSSSN
SMGMTGDLTKKVKTADIIANDLGLIAHKQMTGDSNEMKTLGKHDVRINGCEVQGQDTSNFSNSC
LNPEHEGNIQKADKNEQSQDVVPDSRSSSLEQSAALYKNQKLNSSDSCFIAKEENNGPNVTPKE
NFTSSNRSMELEVKKANSSETLANADVKDLMPHFLLPAAGQFDEKSIITLLDLETTKKLKESGC
ALKDKNVVRHFSLKSAQKRKNNPYRTKVKDAAAQCVNDSDLDASKHSAGRLKKKKKTSSSSLKI
LSEQATKHTSISAADMLILDSSSNSMGMTGDLTKKVKTADIIANDLGLIAHKQMTGDSNEMKTL
GKHDVRINGCEVQGQDTSNFSNSCLNPEHEGNIQKADK(-)
MKEIFRKQIRMSNLRMLSQIQGLVL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	26798616-26793524(-) 26793551-26798743(-)
Potential amino acid sequence	MELEVKKANSSETLANADVKDLMPHFLLPAAGQFDEKSIITLLDLETTKKLKESGCALKDKNVV RHFSLKSAQKRKNNPYRTKVKDAAAQCVNDSDLDASKHSAGRLKKKKKTSSSSLKILSEQATKH TSISAADMLILDSSSNSMGMTGDLTKKVKTADIIANDLGLIAHKQMTGDSNEMKTLGKHDVRIN GCEVQGQDTSNFSNSCLNPEHEGNIQKADKNEQSQDVVPDSRSSSLEQSAALYKNQKLNSSDSC FIAKEENNGPNVTPKENFTSSNRSMELEVKKANSSETLANADVKDLMPHFLLPAAGQFDEKSII TLLDLETTKKLKESGCALKDKNVVRHFSLKSAQKRKNNPYRTKVKDAAAQCVNDSDLDASKHSA GRLKKKKKTSSSSLKILSEQATKHTSISAADMLILDSSSNSMGMTGDLTKKVKTADIIANDLGL IAHKQMTGDSNEMKTLGKHDVRINGCEVQGQDTSNFSNSCLNPEHEGNIQKADKNEQSQDVVPD SRSSSLEQSAALYKNQKLNSSDSCFIAKEENNGPNVTPKENFTSSNRSMELEVKKANSSETLAN ADVKDLMPHFLLPAAGQFDEKSIITLLDLETTKKLKESGCALKDKNVVRHFSLKSAQKRKNNPY RTKVKDAAAQCVNDSDLDASKHSAGRLKKKKKTSSSSLKILSEQATKHTSISAADMLILDSSSN SMGMTGDLTKKVKTADIIANDLGLIAHKQMTGDSNEMKTLGKHDVRINGCEVQGQDTSNFSNSC LNPEHEGNIQKADKNEQSQDVVPDSRSSSLEQSAALYKNQKLNSSDSCFIAKEENNGPNVTPKE NFTSSNRSMELEVKKANSSETLANADVKDLMPHFLLPAAGQFDEKSIITLLDLETTKKLKESGC ALKDKNVVRHFSLKSAQKRKNNPYRTKVKDAAAQCVNDSDLDASKHSAGRLKKKKKTSSSSLKI LSEQATKHTSISAADMLILDSSSNSMGMTGDLTKKVKTADIIANDLGLIAHKQMTGDSNEMKTL GKHDVRINGCEVQGQDTSNFSNSCLNPEHEGNIQKADK(-) MKEIFRKQIRMSNLRMLSQIQGLVL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.002G146100_circ_g.2

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