Plant Circular RNA Database

Detail information of AT5G27290_circ_g.1

			
						 General Information
					
						CircRNA Name
						AT5G27290_circ_g.1
					
						ID in PlantcircBase
						ath_circ_040715
					
						Alias
						At_ciR757, Ath_circ_FC3540
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 9618869-9619517  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, find_circ
					
						Parent gene
						
							AT5G27290						
					
						Parent gene annotation
						
							At5g27290
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT5G27290_circ_g.2 						
					
						Support reads
						11/1
					
						Tissues
						leaf/aerial, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G27290.1:4
AT5G27290.2:4

General Information
CircRNA Name	AT5G27290_circ_g.1
ID in PlantcircBase	ath_circ_040715
Alias	At_ciR757, Ath_circ_FC3540
Organism	Arabidpsis thaliana
Position	chr5: 9618869-9619517 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, find_circ
Parent gene	AT5G27290
Parent gene annotation	At5g27290
Parent gene strand	-
Alternative splicing	AT5G27290_circ_g.2
Support reads	11/1
Tissues	leaf/aerial, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G27290.1:4 AT5G27290.2:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTGCAGGTTTTACCTGAACAACTCTGTTATGGTATCGTTGGCTAAACGTATGACCAGTTGTATC
AAGAACCAAACTCCCAATTCCGCCATTAAAAGAGACcttgctgatatcgtcaaggcctccttca
gcataaccatacaggagatattctgttgctacaccagccagagcaatgcatgagaatctgttca
gcatctgg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 357
Transcript exons: 9618869-9618964,9619056-9619082,9619180-9619326,9619431-
9619517
CTTGCTGATATCGTCAAGGCCTCCTTCAGCATAACCATACAGGAGATATTCTGTTGCTACACCA
GCCAGAGCAATGCATGAGAATCTGTTCAGCATCGTAGCGGAGACTTTTCCAGAATTAACTTCTT
CAAGGAATTCATAGTCTACAAAAGCTGAGCCAGCTTGAATGTTGAGAGATCCTTCTTTCTGTAA
AGCTTCAAGGCTAGAGAGCGTGTATCCGCGTGGTAGAATCCCGACTAAGTAGGCCACCAAGAAA
TGACCCGCTTCGTGCTGAACAACTCTGTTATGGTATCGTTGGCTAAACGTATGACCAGTTGTAT
CAAGAACCAAACTCCCAATTCCGCCATTAAAAGAGAC
Transcript length: 649
Transcript exons: 9618869-9619517
GGTTCTTGATACAACTGGTCATACGTTTAGCCAACGATACCATAACAGAGTTGTTCAGGTCTCT
TTTAATGGCGGAATTGGGAGTTT
						
						Genomic sequence
						
							CTTGCTGATATCGTCAAGGCCTCCTTCAGCATAACCATACAGGAGATATTCTGTTGCTACACCA
GCCAGAGCAATGCATGAGAATCTGTTCAGCATCTGGTTTTGGAATAGCAAACACAGTTTCTTGT
TAGGCTGAAGATTTTAAAAAGGTTTAGAACTTTTTTATGGGAAGTATAAGAAAACATACCGTAG
CGGAGACTTTTCCAGAATTAACCTGCATTGTCAAGTTGGGAAAAAAGTGTTAGAAGCTTGGATT
TGAATGAGTTTCTGCTTCTTCAGAGGATGTGAAAGAAGAAAGAGAGGTTATGTACTTCTTCAAG
GAATTCATAGTCTACAAAAGCTGAGCCAGCTTGAATGTTGAGAGATCCTTCTTTCTGTAAAGCT
TCAAGGCTAGAGAGCGTGTATCCGCGTGGTAGAATCCCGACTAAGTAGGCCACCAAGAAATGAC
CCGCTTCGTGCTGAGATCTCAGTTCACAGTAAGTCCAATGAAAAATGATATAGTGTTTCTAAAT
TTTTTCATAAAGAGTGATATATTGAAGCAGAGTGAAGTTGCAGGTTTTACCTGAACAACTCTGT
TATGGTATCGTTGGCTAAACGTATGACCAGTTGTATCAAGAACCAAACTCCCAATTCCGCCATT
AAAAGAGAC

Sequences
Splice junction sequence	TTGCAGGTTTTACCTGAACAACTCTGTTATGGTATCGTTGGCTAAACGTATGACCAGTTGTATC AAGAACCAAACTCCCAATTCCGCCATTAAAAGAGACcttgctgatatcgtcaaggcctccttca gcataaccatacaggagatattctgttgctacaccagccagagcaatgcatgagaatctgttca gcatctgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 357 Transcript exons: 9618869-9618964,9619056-9619082,9619180-9619326,9619431- 9619517 CTTGCTGATATCGTCAAGGCCTCCTTCAGCATAACCATACAGGAGATATTCTGTTGCTACACCA GCCAGAGCAATGCATGAGAATCTGTTCAGCATCGTAGCGGAGACTTTTCCAGAATTAACTTCTT CAAGGAATTCATAGTCTACAAAAGCTGAGCCAGCTTGAATGTTGAGAGATCCTTCTTTCTGTAA AGCTTCAAGGCTAGAGAGCGTGTATCCGCGTGGTAGAATCCCGACTAAGTAGGCCACCAAGAAA TGACCCGCTTCGTGCTGAACAACTCTGTTATGGTATCGTTGGCTAAACGTATGACCAGTTGTAT CAAGAACCAAACTCCCAATTCCGCCATTAAAAGAGAC Transcript length: 649 Transcript exons: 9618869-9619517 GGTTCTTGATACAACTGGTCATACGTTTAGCCAACGATACCATAACAGAGTTGTTCAGGTCTCT TTTAATGGCGGAATTGGGAGTTT
Genomic sequence	CTTGCTGATATCGTCAAGGCCTCCTTCAGCATAACCATACAGGAGATATTCTGTTGCTACACCA GCCAGAGCAATGCATGAGAATCTGTTCAGCATCTGGTTTTGGAATAGCAAACACAGTTTCTTGT TAGGCTGAAGATTTTAAAAAGGTTTAGAACTTTTTTATGGGAAGTATAAGAAAACATACCGTAG CGGAGACTTTTCCAGAATTAACCTGCATTGTCAAGTTGGGAAAAAAGTGTTAGAAGCTTGGATT TGAATGAGTTTCTGCTTCTTCAGAGGATGTGAAAGAAGAAAGAGAGGTTATGTACTTCTTCAAG GAATTCATAGTCTACAAAAGCTGAGCCAGCTTGAATGTTGAGAGATCCTTCTTTCTGTAAAGCT TCAAGGCTAGAGAGCGTGTATCCGCGTGGTAGAATCCCGACTAAGTAGGCCACCAAGAAATGAC CCGCTTCGTGCTGAGATCTCAGTTCACAGTAAGTCCAATGAAAAATGATATAGTGTTTCTAAAT TTTTTCATAAAGAGTGATATATTGAAGCAGAGTGAAGTTGCAGGTTTTACCTGAACAACTCTGT TATGGTATCGTTGGCTAAACGTATGACCAGTTGTATCAAGAACCAAACTCCCAATTCCGCCATT AAAAGAGAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.383256998

Conservation Information
Conserved circRNAs	NA
PMCS	0.383256998

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9618965-9618871(-)
						
						Potential amino acid sequence
						
							MLNRFSCIALAGVATEYLLYGYAEGGLDDISKVSFNGGIGSLVLDTTGHTFSQRYHNRVVQHEA
GHFLVAYLVGILPRGYTLSSLEALQKEGSLNIQAGSAFVDYEFLEEVNSGKVSATMLNRFSCIA
LAGVATEYLLYGYAEGGLDDISKVSFNGGIGSLVLDTTGHTFSQRYHNRVVQHEAGHFLVAYLV
GILPRGYTLSSLEALQKEGSLNIQAGSAFVDYEFLEEVNSGKVSATMLNRFSCIALAGVATEYL
LYGYAEGGLDDISKVSFNGGIGSLVLDTTGHTFSQRYHNRVVQHEAGHFLVAYLVGILPRGYTL
SSLEALQKEGSLNIQAGSAFVDYEFLEEVNSGKVSATMLNRFSCIALAGVATEYLLYGYAEGGL
DDISK(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	9618965-9618871(-)
Potential amino acid sequence	MLNRFSCIALAGVATEYLLYGYAEGGLDDISKVSFNGGIGSLVLDTTGHTFSQRYHNRVVQHEA GHFLVAYLVGILPRGYTLSSLEALQKEGSLNIQAGSAFVDYEFLEEVNSGKVSATMLNRFSCIA LAGVATEYLLYGYAEGGLDDISKVSFNGGIGSLVLDTTGHTFSQRYHNRVVQHEAGHFLVAYLV GILPRGYTLSSLEALQKEGSLNIQAGSAFVDYEFLEEVNSGKVSATMLNRFSCIALAGVATEYL LYGYAEGGLDDISKVSFNGGIGSLVLDTTGHTFSQRYHNRVVQHEAGHFLVAYLVGILPRGYTL SSLEALQKEGSLNIQAGSAFVDYEFLEEVNSGKVSATMLNRFSCIALAGVATEYLLYGYAEGGL DDISK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT5G27290_circ_g.1

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