Plant Circular RNA Database

Detail information of Os01g0679000_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os01g0679000_circ_g.4
					
						ID in PlantcircBase
						osa_circ_003232
					
						Alias
						Os_ciR6003
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 27945843-27945992  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os01g0679000						
					
						Parent gene annotation
						
							RNA polymerase III Rpc82, C -terminal domain containing protein.
 (Os01t0679000-01);Hypothetical conserved gene. (Os01t0679000-02
)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2/1
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0679000-02:1
Os01t0679000-01:1

General Information
CircRNA Name	Os01g0679000_circ_g.4
ID in PlantcircBase	osa_circ_003232
Alias	Os_ciR6003
Organism	Oryza sativa
Position	chr1: 27945843-27945992 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os01g0679000
Parent gene annotation	RNA polymerase III Rpc82, C -terminal domain containing protein. (Os01t0679000-01);Hypothetical conserved gene. (Os01t0679000-02 )
Parent gene strand	+
Alternative splicing	NA
Support reads	2/1
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0679000-02:1 Os01t0679000-01:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTCATCGATAATCTATGTCATGCTGCTTTAAACCTGCGGCAAATGGTCAATTACATAGCTGAAC
TCCTACTCGAGagaatcctttctggaacaggaacagggaacacacaatattttgtatggagagt
gaaaaatacattccgggaacag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 150
Transcript exons: 27945843-27945992
AGAATCCTTTCTGGAACAGGAACAGGGAACACACAATATTTTGTATGGAGAGTGAAAAATACAT
TCCGGGAACAGTTCATCGATAATCTATGTCATGCTGCTTTAAACCTGCGGCAAATGGTCAATTA
CATAGCTGAACTCCTACTCGAG
						
						Genomic sequence
						
							AGAATCCTTTCTGGAACAGGAACAGGGAACACACAATATTTTGTATGGAGAGTGAAAAATACAT
TCCGGGAACAGTTCATCGATAATCTATGTCATGCTGCTTTAAACCTGCGGCAAATGGTCAATTA
CATAGCTGAACTCCTACTCGAG

Sequences
Splice junction sequence	TTCATCGATAATCTATGTCATGCTGCTTTAAACCTGCGGCAAATGGTCAATTACATAGCTGAAC TCCTACTCGAGagaatcctttctggaacaggaacagggaacacacaatattttgtatggagagt gaaaaatacattccgggaacag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 150 Transcript exons: 27945843-27945992 AGAATCCTTTCTGGAACAGGAACAGGGAACACACAATATTTTGTATGGAGAGTGAAAAATACAT TCCGGGAACAGTTCATCGATAATCTATGTCATGCTGCTTTAAACCTGCGGCAAATGGTCAATTA CATAGCTGAACTCCTACTCGAG
Genomic sequence	AGAATCCTTTCTGGAACAGGAACAGGGAACACACAATATTTTGTATGGAGAGTGAAAAATACAT TCCGGGAACAGTTCATCGATAATCTATGTCATGCTGCTTTAAACCTGCGGCAAATGGTCAATTA CATAGCTGAACTCCTACTCGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.272218778

Conservation Information
Conserved circRNAs	NA
PMCS	0.272218778

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							27945961-27945989(+)
27945924-27945970(-)
27945908-27945966(-)
						
						Potential amino acid sequence
						
							MVNYIAELLLERILSGTGTGNTQYFVWRVKNTFREQFIDNLCHAALNLRQMVNYIAELLLERIL
SGTGTGNTQYFVWRVKNTFREQFIDNLCHAALNLRQMVNYIAELLLERILSGTGTGNTQYFVWR
VKNTFREQFIDNLCHAALNLRQMVNYIAELLLE(+)
MNCSRNVFFTLHTKYCVFPVPVPERILSSRSSAM*(-)
MYFSLSIQNIVCSLFLFQKGFSRVGVQLCN*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	27945961-27945989(+) 27945924-27945970(-) 27945908-27945966(-)
Potential amino acid sequence	MVNYIAELLLERILSGTGTGNTQYFVWRVKNTFREQFIDNLCHAALNLRQMVNYIAELLLERIL SGTGTGNTQYFVWRVKNTFREQFIDNLCHAALNLRQMVNYIAELLLERILSGTGTGNTQYFVWR VKNTFREQFIDNLCHAALNLRQMVNYIAELLLE(+) MNCSRNVFFTLHTKYCVFPVPVPERILSSRSSAM(-) MYFSLSIQNIVCSLFLFQKGFSRVGVQLCN(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os01g0679000_circ_g.4

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