Plant Circular RNA Database

Detail information of Os07g0421300_circ_g.12

			
						 General Information
					
						CircRNA Name
						Os07g0421300_circ_g.12
					
						ID in PlantcircBase
						osa_circ_033554
					
						Alias
						Os_ciR4954
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr7: 13546543-13547867  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os07g0421300						
					
						Parent gene annotation
						
							Similar to Alpha glucosidase-like protein. (Os07t0421300-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os07g0421300_circ_g.10 Os07g0421300_circ_g.11 						
					
						Support reads
						4
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os07t0421300-01:5

General Information
CircRNA Name	Os07g0421300_circ_g.12
ID in PlantcircBase	osa_circ_033554
Alias	Os_ciR4954
Organism	Oryza sativa
Position	chr7: 13546543-13547867 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os07g0421300
Parent gene annotation	Similar to Alpha glucosidase-like protein. (Os07t0421300-01)
Parent gene strand	+
Alternative splicing	Os07g0421300_circ_g.10 Os07g0421300_circ_g.11
Support reads	4
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os07t0421300-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AGCGCCAAATATCCATTTGTAAGGAAAACCCAAAGATTGTTAAGATCGACTCCAGTATTCGAGC
AAAACAGGGAGCTGATCATTCTGGTGGGTTTTCAGGgaaaagctgaaggtgtcctctttgaaga
tgatggggatggatatgagttccttcaagggaattatcttttgacatactatgttgcagaactc
cactcatc

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 733
Transcript exons: 13546543-13546736,13546818-13546926,13547067-13547222,13
547437-13547536,13547694-13547867
GAAAAGCTGAAGGTGTCCTCTTTGAAGATGATGGGGATGGATATGAGTTCCTTCAAGGGAATTA
TCTTTTGACATACTATGTTGCAGAACTCCACTCATCAGTGGTTACTGTTAAAGTTGCCCGAACT
GAAGGATCATGGAAGAGACCAAATCGCAACTTAAAAATAAATATATTGCTTGGAGGAGGTGCTA
TGGTAAGTACGCACGGCATTGATGGGGAAGATTTACATCTAACAATGCCTACTGAATCTGAAGT
GTCAAGTCTAGTGGCAACAAGTGAACTTGAGCTCAAAAAACGTTTTGAGATGGTTCGACCTATT
CCAGACATAGATAAGCCATTAGGGAAGGAAGTGGCTGAACTTTCTGAAATACCTATTGATTTGA
ACGGCGAGGACTGGTTGGTTAAAGTTGTACCCCAGATCGGTGGTCGTATTATCTCAATGACACA
TCTTCCATCTGATTCTCAATGGCTACACAGCACAAATAGGATCAATGGGTATGAGGAATATAAT
GCGGCTGAAGATACTGCTGGCTGCACAGAAGAGTACAAAGTCATTAGGAGGTATCGCGAGCAAT
CAGGAAAAGAGGAATCTATTTGTTTGGAAGGAGATATTGGGGGTGGTTTGGTTCTTCAGCGCCA
AATATCCATTTGTAAGGAAAACCCAAAGATTGTTAAGATCGACTCCAGTATTCGAGCAAAACAG
GGAGCTGATCATTCTGGTGGGTTTTCAGG
						
					

						Genomic sequence
						
							GAAAAGCTGAAGGTGTCCTCTTTGAAGATGATGGGGATGGATATGAGTTCCTTCAAGGGAATTA
TCTTTTGACATACTATGTTGCAGAACTCCACTCATCAGTGGTTACTGTTAAAGTTGCCCGAACT
GAAGGATCATGGAAGAGACCAAATCGCAACTTAAAAATAAATATATTGCTTGGAGGAGGTGCTA
TGGTAGGTCCATTATATTAGTTCCTTCTTGTTTTTGTCAAATGTGCACAGATGAATATTAAATT
ATAAGTTTTTCAAATACAGGTAAGTACGCACGGCATTGATGGGGAAGATTTACATCTAACAATG
CCTACTGAATCTGAAGTGTCAAGTCTAGTGGCAACAAGTGAACTTGAGCTCAAAAAACGTTTTG
GTATTAGTCCTACACACTTGCACAGTACTCAAAATGTTTTAGAACAGGGAATTCTTACATCTCC
AGTTTTTCCATTTATTTATTCCGAAATATTTTATTTGTGGGCTTACTTTAGTATCTTCCTACCA
TTCATTATACAGAGATGGTTCGACCTATTCCAGACATAGATAAGCCATTAGGGAAGGAAGTGGC
TGAACTTTCTGAAATACCTATTGATTTGAACGGCGAGGACTGGTTGGTTAAAGTTGTACCCCAG
ATCGGTGGTCGTATTATCTCAATGACACATCTTCCATCTGGTATTGCTCTTATTGATTTATATT
ACTTTCTGTTGGTTATGCCTCATACGATGTGTTTAGAGATAGATAATCCTATTACTGCATCTGG
TCGAAAATACAAGTCTTTTGAAGATATGTGTCAGTTAAACTTTTCTAGCTTTAGTTAATTATCA
TATCCATGTAAATAATTACATCGATTGGAAATGTATATATAATTTATACTTGATCTCTGCAGAT
TCTCAATGGCTACACAGCACAAATAGGATCAATGGGTATGAGGAATATAATGCGGCTGAAGATA
CTGCTGGCTGCACAGAAGAGTACAAAGTCATTAGGTAGGAGAAATGCAGAGTACTTCATTACTT
ATTCAGTGTTCACTCTAACCTAGTTGGCGGCACTTATGGCACTGATCTGTAGAAGAATATGTGA
CTGGTGGTGTATGAGTGTTATTGCTTAAATTCATGTTTTGACATAATTTCTAAAAATGCATAGG
AGGTATCGCGAGCAATCAGGAAAAGAGGAATCTATTTGTTTGGAAGGAGATATTGGGGGTGGTT
TGGTTCTTCAGCGCCAAATATCCATTTGTAAGGAAAACCCAAAGATTGTTAAGATCGACTCCAG
TATTCGAGCAAAACAGGGAGCTGATCATTCTGGTGGGTTTTCAGG
						
					

			
		

Sequences
Splice junction sequence	AGCGCCAAATATCCATTTGTAAGGAAAACCCAAAGATTGTTAAGATCGACTCCAGTATTCGAGC AAAACAGGGAGCTGATCATTCTGGTGGGTTTTCAGGgaaaagctgaaggtgtcctctttgaaga tgatggggatggatatgagttccttcaagggaattatcttttgacatactatgttgcagaactc cactcatc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 733 Transcript exons: 13546543-13546736,13546818-13546926,13547067-13547222,13 547437-13547536,13547694-13547867 GAAAAGCTGAAGGTGTCCTCTTTGAAGATGATGGGGATGGATATGAGTTCCTTCAAGGGAATTA TCTTTTGACATACTATGTTGCAGAACTCCACTCATCAGTGGTTACTGTTAAAGTTGCCCGAACT GAAGGATCATGGAAGAGACCAAATCGCAACTTAAAAATAAATATATTGCTTGGAGGAGGTGCTA TGGTAAGTACGCACGGCATTGATGGGGAAGATTTACATCTAACAATGCCTACTGAATCTGAAGT GTCAAGTCTAGTGGCAACAAGTGAACTTGAGCTCAAAAAACGTTTTGAGATGGTTCGACCTATT CCAGACATAGATAAGCCATTAGGGAAGGAAGTGGCTGAACTTTCTGAAATACCTATTGATTTGA ACGGCGAGGACTGGTTGGTTAAAGTTGTACCCCAGATCGGTGGTCGTATTATCTCAATGACACA TCTTCCATCTGATTCTCAATGGCTACACAGCACAAATAGGATCAATGGGTATGAGGAATATAAT GCGGCTGAAGATACTGCTGGCTGCACAGAAGAGTACAAAGTCATTAGGAGGTATCGCGAGCAAT CAGGAAAAGAGGAATCTATTTGTTTGGAAGGAGATATTGGGGGTGGTTTGGTTCTTCAGCGCCA AATATCCATTTGTAAGGAAAACCCAAAGATTGTTAAGATCGACTCCAGTATTCGAGCAAAACAG GGAGCTGATCATTCTGGTGGGTTTTCAGG
Genomic sequence	GAAAAGCTGAAGGTGTCCTCTTTGAAGATGATGGGGATGGATATGAGTTCCTTCAAGGGAATTA TCTTTTGACATACTATGTTGCAGAACTCCACTCATCAGTGGTTACTGTTAAAGTTGCCCGAACT GAAGGATCATGGAAGAGACCAAATCGCAACTTAAAAATAAATATATTGCTTGGAGGAGGTGCTA TGGTAGGTCCATTATATTAGTTCCTTCTTGTTTTTGTCAAATGTGCACAGATGAATATTAAATT ATAAGTTTTTCAAATACAGGTAAGTACGCACGGCATTGATGGGGAAGATTTACATCTAACAATG CCTACTGAATCTGAAGTGTCAAGTCTAGTGGCAACAAGTGAACTTGAGCTCAAAAAACGTTTTG GTATTAGTCCTACACACTTGCACAGTACTCAAAATGTTTTAGAACAGGGAATTCTTACATCTCC AGTTTTTCCATTTATTTATTCCGAAATATTTTATTTGTGGGCTTACTTTAGTATCTTCCTACCA TTCATTATACAGAGATGGTTCGACCTATTCCAGACATAGATAAGCCATTAGGGAAGGAAGTGGC TGAACTTTCTGAAATACCTATTGATTTGAACGGCGAGGACTGGTTGGTTAAAGTTGTACCCCAG ATCGGTGGTCGTATTATCTCAATGACACATCTTCCATCTGGTATTGCTCTTATTGATTTATATT ACTTTCTGTTGGTTATGCCTCATACGATGTGTTTAGAGATAGATAATCCTATTACTGCATCTGG TCGAAAATACAAGTCTTTTGAAGATATGTGTCAGTTAAACTTTTCTAGCTTTAGTTAATTATCA TATCCATGTAAATAATTACATCGATTGGAAATGTATATATAATTTATACTTGATCTCTGCAGAT TCTCAATGGCTACACAGCACAAATAGGATCAATGGGTATGAGGAATATAATGCGGCTGAAGATA CTGCTGGCTGCACAGAAGAGTACAAAGTCATTAGGTAGGAGAAATGCAGAGTACTTCATTACTT ATTCAGTGTTCACTCTAACCTAGTTGGCGGCACTTATGGCACTGATCTGTAGAAGAATATGTGA CTGGTGGTGTATGAGTGTTATTGCTTAAATTCATGTTTTGACATAATTTCTAAAAATGCATAGG AGGTATCGCGAGCAATCAGGAAAAGAGGAATCTATTTGTTTGGAAGGAGATATTGGGGGTGGTT TGGTTCTTCAGCGCCAAATATCCATTTGTAAGGAAAACCCAAAGATTGTTAAGATCGACTCCAG TATTCGAGCAAAACAGGGAGCTGATCATTCTGGTGGGTTTTCAGG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.228214044

Conservation Information
Conserved circRNAs	NA
PMCS	0.228214044

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13547471-13546549(+)
13546735-13546549(+)
13546643-13547849(-)
						
						Potential amino acid sequence
						
							MGMRNIMRLKILLAAQKSTKSLGGIASNQEKRNLFVWKEILGVVWFFSAKYPFVRKTQRLLRST
PVFEQNRELIILVGFQGKAEGVLFEDDGDGYEFLQGNYLLTYYVAELHSSVVTVKVARTEGSWK
RPNRNLKINILLGGGAMVSTHGIDGEDLHLTMPTESEVSSLVATSELELKKRFEMVRPIPDIDK
PLGKEVAELSEIPIDLNGEDWLVKVVPQIGGRIISMTHLPSDSQWLHSTNRINGYEEYNAAEDT
AGCTEEYKVIRRYREQSGKEESICLEGDIGGGLVLQRQISICKENPKIVKIDSSIRAKQGADHS
GGFSGKS*(+)
MVSTHGIDGEDLHLTMPTESEVSSLVATSELELKKRFEMVRPIPDIDKPLGKEVAELSEIPIDL
NGEDWLVKVVPQIGGRIISMTHLPSDSQWLHSTNRINGYEEYNAAEDTAGCTEEYKVIRRYREQ
SGKEESICLEGDIGGGLVLQRQISICKENPKIVKIDSSIRAKQGADHSGGFSGKS*(+)
MSGVLQHSMSKDNSLEGTHIHPHHLQRGHLQLFPENPPE*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13547471-13546549(+) 13546735-13546549(+) 13546643-13547849(-)
Potential amino acid sequence	MGMRNIMRLKILLAAQKSTKSLGGIASNQEKRNLFVWKEILGVVWFFSAKYPFVRKTQRLLRST PVFEQNRELIILVGFQGKAEGVLFEDDGDGYEFLQGNYLLTYYVAELHSSVVTVKVARTEGSWK RPNRNLKINILLGGGAMVSTHGIDGEDLHLTMPTESEVSSLVATSELELKKRFEMVRPIPDIDK PLGKEVAELSEIPIDLNGEDWLVKVVPQIGGRIISMTHLPSDSQWLHSTNRINGYEEYNAAEDT AGCTEEYKVIRRYREQSGKEESICLEGDIGGGLVLQRQISICKENPKIVKIDSSIRAKQGADHS GGFSGKS(+) MVSTHGIDGEDLHLTMPTESEVSSLVATSELELKKRFEMVRPIPDIDKPLGKEVAELSEIPIDL NGEDWLVKVVPQIGGRIISMTHLPSDSQWLHSTNRINGYEEYNAAEDTAGCTEEYKVIRRYREQ SGKEESICLEGDIGGGLVLQRQISICKENPKIVKIDSSIRAKQGADHSGGFSGKS(+) MSGVLQHSMSKDNSLEGTHIHPHHLQRGHLQLFPENPPE*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os07g0421300_circ_g.12

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