Plant Circular RNA Database

Detail information of Zm00001d048540_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d048540_circ_g.2
					
						ID in PlantcircBase
						zma_circ_010119
					
						Alias
						zma_circ_0003146
					
						Organism
						
							Zea mays						
					
						Position
						
							chr9: 158121430-158121888  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Zm00001d048540						
					
						Parent gene annotation
						
							Phosphatidylinositol N-acetyglucosaminlytransferase subunit P-re
lated
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d048540_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d048540_T005:2
Zm00001d048540_T004:2
Zm00001d048540_T009:2
Zm00001d048540_T002:2
Zm00001d048540_T008:2
Zm00001d048540_T007:2

General Information
CircRNA Name	Zm00001d048540_circ_g.2
ID in PlantcircBase	zma_circ_010119
Alias	zma_circ_0003146
Organism	Zea mays
Position	chr9: 158121430-158121888 JBrowse»
Reference genome	AGPv4.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Zm00001d048540
Parent gene annotation	Phosphatidylinositol N-acetyglucosaminlytransferase subunit P-re lated
Parent gene strand	+
Alternative splicing	Zm00001d048540_circ_g.1
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d048540_T005:2 Zm00001d048540_T004:2 Zm00001d048540_T009:2 Zm00001d048540_T002:2 Zm00001d048540_T008:2 Zm00001d048540_T007:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTCAGACCAAGGGATTTTCTGGGAGGACAAGCATCCGAGGTTATGTTACATAAAGGAATTGCTA
GAGCTGTCAGAATTGTGTGCAAACCAGAACTTAGAGgatatggtgggagaagcagagccaggac
aaaggcatgtcaggacgtttccaagctcccctgagaacgactttgaaaaactggaatgccaaga
gcccacga

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GATATGGTGGGAGAAGCAGAGCCAGGACAAAGGCATGTCAGGACGTTTCCAAGCTCCCCTGAGA
ACGACTTTGAAAAACTGGAATGCCAAGAGCCCACGACTCCCCGACCGAGTGCACAGATCGAACA
GATATCACAATTTTCTCCTGATGGAAACCACGAAAAGCAAGAACAGCCGAGCCCTCAATCTGTT
CTTGATGTGTTCTTCCTCGACGGTACTGACACTGAAAACATGATAGAATGTAAACTCATCAATT
CACTCCAAGTCAAACTTAAATGATTTATTTTGGTGCTCCAATTGTCTACAGGTGAGTTGCACAA
AGATATCTTGAGGACACGCTACAACACCACAGGGGATGGTTCAGACCAAGGGATTTTCTGGGAG
GACAAGCATCCGAGGTTATGTTACATAAAGGAATTGCTAGAGCTGTCAGAATTGTGTGCAAACC
AGAACTTAGAG

Sequences
Splice junction sequence	TTCAGACCAAGGGATTTTCTGGGAGGACAAGCATCCGAGGTTATGTTACATAAAGGAATTGCTA GAGCTGTCAGAATTGTGTGCAAACCAGAACTTAGAGgatatggtgggagaagcagagccaggac aaaggcatgtcaggacgtttccaagctcccctgagaacgactttgaaaaactggaatgccaaga gcccacga
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GATATGGTGGGAGAAGCAGAGCCAGGACAAAGGCATGTCAGGACGTTTCCAAGCTCCCCTGAGA ACGACTTTGAAAAACTGGAATGCCAAGAGCCCACGACTCCCCGACCGAGTGCACAGATCGAACA GATATCACAATTTTCTCCTGATGGAAACCACGAAAAGCAAGAACAGCCGAGCCCTCAATCTGTT CTTGATGTGTTCTTCCTCGACGGTACTGACACTGAAAACATGATAGAATGTAAACTCATCAATT CACTCCAAGTCAAACTTAAATGATTTATTTTGGTGCTCCAATTGTCTACAGGTGAGTTGCACAA AGATATCTTGAGGACACGCTACAACACCACAGGGGATGGTTCAGACCAAGGGATTTTCTGGGAG GACAAGCATCCGAGGTTATGTTACATAAAGGAATTGCTAGAGCTGTCAGAATTGTGTGCAAACC AGAACTTAGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.110203159

Conservation Information
Conserved circRNAs	NA
PMCS	0.110203159

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							158121434-158121885(+)
158121832-158121488(+)
						
						Potential amino acid sequence
						
							MVGEAEPGQRHVRTFPSSPENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVL
DVFFLDGELHKDILRTRYNTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLEDMVGEAE
PGQRHVRTFPSSPENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVLDVFFLD
GELHKDILRTRYNTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLEDMVGEAEPGQRHV
RTFPSSPENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVLDVFFLDGELHKD
ILRTRYNTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLEDMVGEAEPGQRHVRTFPSS
PENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVLDVFFLDGELHKDILRTRY
NTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLE(+)
MLHKGIARAVRIVCKPELRGYGGRSRARTKACQDVSKLP*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	158121434-158121885(+) 158121832-158121488(+)
Potential amino acid sequence	MVGEAEPGQRHVRTFPSSPENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVL DVFFLDGELHKDILRTRYNTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLEDMVGEAE PGQRHVRTFPSSPENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVLDVFFLD GELHKDILRTRYNTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLEDMVGEAEPGQRHV RTFPSSPENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVLDVFFLDGELHKD ILRTRYNTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLEDMVGEAEPGQRHVRTFPSS PENDFEKLECQEPTTPRPSAQIEQISQFSPDGNHEKQEQPSPQSVLDVFFLDGELHKDILRTRY NTTGDGSDQGIFWEDKHPRLCYIKELLELSELCANQNLE(+) MLHKGIARAVRIVCKPELRGYGGRSRARTKACQDVSKLP*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ma et al., 2021b

circRNA

Detail information of Zm00001d048540_circ_g.2

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