Plant Circular RNA Database

Detail information of Os07g0508300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os07g0508300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_034001
					
						Alias
						Os07circ09012
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr7: 19310760-19311005  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os07g0508300						
					
						Parent gene annotation
						
							Similar to SH3 domain-containing protein 3. (Os07t0508300-01);Si
milar to clathrin binding protein. (Os07t0508300-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						leaf and panicle
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os07t0508250-00:2
Os07t0508300-02:2
Os07t0508250-00:2
Os07t0508300-01:2

General Information
CircRNA Name	Os07g0508300_circ_g.1
ID in PlantcircBase	osa_circ_034001
Alias	Os07circ09012
Organism	Oryza sativa
Position	chr7: 19310760-19311005 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os07g0508300
Parent gene annotation	Similar to SH3 domain-containing protein 3. (Os07t0508300-01);Si milar to clathrin binding protein. (Os07t0508300-02)
Parent gene strand	-
Alternative splicing	NA
Support reads	2
Tissues	leaf and panicle
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os07t0508250-00:2 Os07t0508300-02:2 Os07t0508250-00:2 Os07t0508300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCATGTTAACCTCAGCAAGGAAGTACTGGGCCTTCTCAGCACGCTTATGGGAATGAATAGTAGG
CGGTGCAGATTCTCTCTTTTGCTTTTCAGAGGACATctgacgaacaacgacataatcaccaaca
attaaactcaactccttttctgtggtaccattgaagttatgcaccgcctgcagcaacaagaagg
ttcaatgc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CTGACGAACAACGACATAATCACCAACAATTAAACTCAACTCCTTTTCTGTGGTACCATTGAAG
TTATGCACCGCCTGCAGCAACAAGAAGGTTCAATGCACATGACATGTAAAATATGGTATAATTG
CTTGGTAATGTCAGTTACTCATGTTAACCTCAGCAAGGAAGTACTGGGCCTTCTCAGCACGCTT
ATGGGAATGAATAGTAGGCGGTGCAGATTCTCTCTTTTGCTTTTCAGAGGACAT

Sequences
Splice junction sequence	TCATGTTAACCTCAGCAAGGAAGTACTGGGCCTTCTCAGCACGCTTATGGGAATGAATAGTAGG CGGTGCAGATTCTCTCTTTTGCTTTTCAGAGGACATctgacgaacaacgacataatcaccaaca attaaactcaactccttttctgtggtaccattgaagttatgcaccgcctgcagcaacaagaagg ttcaatgc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTGACGAACAACGACATAATCACCAACAATTAAACTCAACTCCTTTTCTGTGGTACCATTGAAG TTATGCACCGCCTGCAGCAACAAGAAGGTTCAATGCACATGACATGTAAAATATGGTATAATTG CTTGGTAATGTCAGTTACTCATGTTAACCTCAGCAAGGAAGTACTGGGCCTTCTCAGCACGCTT ATGGGAATGAATAGTAGGCGGTGCAGATTCTCTCTTTTGCTTTTCAGAGGACAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.194139092

Conservation Information
Conserved circRNAs	NA
PMCS	0.194139092

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							19310827-19310789(+)
19311007-19310762(-)
19310778-19310797(-)
						
						Potential amino acid sequence
						
							MHRLSKEVLGLLSTLMGMNSRRCRFSLLLFRGHLTNNDIITNN*(+)
MSSEKQKRESAPPTIHSHKRAEKAQYFLAEAVHNFNGTTEKELSLIVGDYVVVRQMSSEKQKRE
SAPPTIHSHKRAEKAQYFLAEAVHNFNGTTEKELSLIVGDYVVVRQMSSEKQKRESAPPTIHSH
KRAEKAQYFLAEAVHNFNGTTEKELSLIVGDYVVVRQMSSEKQKRESAPPTIHSHKRAEKAQYF
LAEAVHNFNGTTEKELSLIVGDYVVVRQ(-)
MSLFVRCPLKSKRENLHRLLFIPISVLRRPSTSLLRRCITSMVPQKRS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	19310827-19310789(+) 19311007-19310762(-) 19310778-19310797(-)
Potential amino acid sequence	MHRLSKEVLGLLSTLMGMNSRRCRFSLLLFRGHLTNNDIITNN(+) MSSEKQKRESAPPTIHSHKRAEKAQYFLAEAVHNFNGTTEKELSLIVGDYVVVRQMSSEKQKRE SAPPTIHSHKRAEKAQYFLAEAVHNFNGTTEKELSLIVGDYVVVRQMSSEKQKRESAPPTIHSH KRAEKAQYFLAEAVHNFNGTTEKELSLIVGDYVVVRQMSSEKQKRESAPPTIHSHKRAEKAQYF LAEAVHNFNGTTEKELSLIVGDYVVVRQ(-) MSLFVRCPLKSKRENLHRLLFIPISVLRRPSTSLLRRCITSMVPQKRS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os07g0508300_circ_g.1

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