Plant Circular RNA Database

Detail information of orai.011G279400_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.011G279400_circ_g.2
					
						ID in PlantcircBase
						gra_circ_001277
					
						Alias
						Chr11:61135458|61135679
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr11: 61135458-61135679  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.011G279400						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							orai.011G279400_circ_g.1 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.011G279400.2:1
Gorai.011G279400.3:1
Gorai.011G279400.1:1

General Information
CircRNA Name	orai.011G279400_circ_g.2
ID in PlantcircBase	gra_circ_001277
Alias	Chr11:61135458\|61135679
Organism	Gossypium raimondii
Position	chrChr11: 61135458-61135679 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.011G279400
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	orai.011G279400_circ_g.1
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.011G279400.2:1 Gorai.011G279400.3:1 Gorai.011G279400.1:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CAAGTGGCTGCCGCGATGGGAATTTTCGGTCCTCGAACAACATATGTTCTCGCACTTAAGGACA
TCCCTGGCACCCATGAATTCCTCCTCCTTGATGAAGgtggattcagtgttgctttcgatccgct
cgatggttccagcattgtcgatacaaatttcaccgtaggtaccatctttggtgtttggcctggt
gataagtt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 222
Transcript exons: 61135458-61135679
GTGGATTCAGTGTTGCTTTCGATCCGCTCGATGGTTCCAGCATTGTCGATACAAATTTCACCGT
AGGTACCATCTTTGGTGTTTGGCCTGGTGATAAGTTAACCGGTGTAACGGGACGAGACCAAGTG
GCTGCCGCGATGGGAATTTTCGGTCCTCGAACAACATATGTTCTCGCACTTAAGGACATCCCTG
GCACCCATGAATTCCTCCTCCTTGATGAAG
						
						Genomic sequence
						
							GTGGATTCAGTGTTGCTTTCGATCCGCTCGATGGTTCCAGCATTGTCGATACAAATTTCACCGT
AGGTACCATCTTTGGTGTTTGGCCTGGTGATAAGTTAACCGGTGTAACGGGACGAGACCAAGTG
GCTGCCGCGATGGGAATTTTCGGTCCTCGAACAACATATGTTCTCGCACTTAAGGACATCCCTG
GCACCCATGAATTCCTCCTCCTTGATGAAG

Sequences
Splice junction sequence	CAAGTGGCTGCCGCGATGGGAATTTTCGGTCCTCGAACAACATATGTTCTCGCACTTAAGGACA TCCCTGGCACCCATGAATTCCTCCTCCTTGATGAAGgtggattcagtgttgctttcgatccgct cgatggttccagcattgtcgatacaaatttcaccgtaggtaccatctttggtgtttggcctggt gataagtt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 222 Transcript exons: 61135458-61135679 GTGGATTCAGTGTTGCTTTCGATCCGCTCGATGGTTCCAGCATTGTCGATACAAATTTCACCGT AGGTACCATCTTTGGTGTTTGGCCTGGTGATAAGTTAACCGGTGTAACGGGACGAGACCAAGTG GCTGCCGCGATGGGAATTTTCGGTCCTCGAACAACATATGTTCTCGCACTTAAGGACATCCCTG GCACCCATGAATTCCTCCTCCTTGATGAAG
Genomic sequence	GTGGATTCAGTGTTGCTTTCGATCCGCTCGATGGTTCCAGCATTGTCGATACAAATTTCACCGT AGGTACCATCTTTGGTGTTTGGCCTGGTGATAAGTTAACCGGTGTAACGGGACGAGACCAAGTG GCTGCCGCGATGGGAATTTTCGGTCCTCGAACAACATATGTTCTCGCACTTAAGGACATCCCTG GCACCCATGAATTCCTCCTCCTTGATGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.756374099

Conservation Information
Conserved circRNAs	NA
PMCS	0.756374099

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							61135623-61135520(+)
61135595-61135676(+)
61135645-61135460(-)
						
						Potential amino acid sequence
						
							MFSHLRTSLAPMNSSSLMKVDSVLLSIRSMVPALSIQISP*(+)
MGIFGPRTTYVLALKDIPGTHEFLLLDEGGFSVAFDPLDGSSIVDTNFTVGTIFGVWPGDKLTG
VTGRDQVAAAMGIFGPRTTYVLALKDIPGTHEFLLLDEGGFSVAFDPLDGSSIVDTNFTVGTIF
GVWPGDKLTGVTGRDQVAAAMGIFGPRTTYVLALKDIPGTHEFLLLDEGGFSVAFDPLDGSSIV
DTNFTVGTIFGVWPGDKLTGVTGRDQVAAAMGIFGPRTTYVLALKDIPGTHEFLLLDE(+)
MSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPGQTPKMVPTVKFVSTMLEPSSGSKATLNPPS
SRRRNSWVPGMSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPGQTPKMVPTVKFVSTMLEPSS
GSKATLNPPSSRRRNSWVPGMSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPGQTPKMVPTVK
FVSTMLEPSSGSKATLNPPSSRRRNSWVPGMSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPG
QTPKMVPTVKFVSTMLEPSSGSKATLNP(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	61135623-61135520(+) 61135595-61135676(+) 61135645-61135460(-)
Potential amino acid sequence	MFSHLRTSLAPMNSSSLMKVDSVLLSIRSMVPALSIQISP*(+) MGIFGPRTTYVLALKDIPGTHEFLLLDEGGFSVAFDPLDGSSIVDTNFTVGTIFGVWPGDKLTG VTGRDQVAAAMGIFGPRTTYVLALKDIPGTHEFLLLDEGGFSVAFDPLDGSSIVDTNFTVGTIF GVWPGDKLTGVTGRDQVAAAMGIFGPRTTYVLALKDIPGTHEFLLLDEGGFSVAFDPLDGSSIV DTNFTVGTIFGVWPGDKLTGVTGRDQVAAAMGIFGPRTTYVLALKDIPGTHEFLLLDE(+) MSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPGQTPKMVPTVKFVSTMLEPSSGSKATLNPPS SRRRNSWVPGMSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPGQTPKMVPTVKFVSTMLEPSS GSKATLNPPSSRRRNSWVPGMSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPGQTPKMVPTVK FVSTMLEPSSGSKATLNPPSSRRRNSWVPGMSLSARTYVVRGPKIPIAAATWSRPVTPVNLSPG QTPKMVPTVKFVSTMLEPSSGSKATLNP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.011G279400_circ_g.2

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