Plant Circular RNA Database

Detail information of Os12g0613600_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os12g0613600_circ_g.1
					
						ID in PlantcircBase
						osa_circ_012298
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr12: 25999419-26000461  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os12g0613600						
					
						Parent gene annotation
						
							Remorin, C-terminal region domain containing protein. (Os12t0613
600-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os12t0613600-01:2

General Information
CircRNA Name	Os12g0613600_circ_g.1
ID in PlantcircBase	osa_circ_012298
Alias	NA
Organism	Oryza sativa
Position	chr12: 25999419-26000461 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os12g0613600
Parent gene annotation	Remorin, C-terminal region domain containing protein. (Os12t0613 600-01)
Parent gene strand	+
Alternative splicing	NA
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os12t0613600-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GACCTCACTCCGGCGACGGCGGCGGCGAGGCCAAGAAGAGGGAGTTCGAGGCGCGCGCCATGGC
GTGGCAGGAGACGCACAAGTGCAAGCTCGCGTTGAGatgggagtgatgtctccaaccaagctga
ggatgaagctgctgggatcccatggcggcagcatcggcagggttgacgaggcaaagaagtcgcc
gcgagcgt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 962
Transcript exons: 25999419-25999596,25999678-26000461
ATGGGAGTGATGTCTCCAACCAAGCTGAGGATGAAGCTGCTGGGATCCCATGGCGGCAGCATCG
GCAGGGTTGACGAGGCAAAGAAGTCGCCGCGAGCGTCGCCGGCGAGGCTCGACGCCGACGAGGA
CGACGACGACCACCCCAAGAACAGCCTCCTGCCCCAAGAACTCGACGAAGATTATCCCAAGGAC
CAGTCGGATTCTTCTCGCTCGCGCTCCGACGCGAGCCATGGGAGAGCTGGCAATGGCTACGACA
GCGGCGGCTTCGAGTTCTACAGGGAGGAGAGGCCGCCGCCACCGCCGCCGGCGGCGGTGGCGGT
GGTCGGCGGGACGTTCTTCAGGCAGGTGCCGTCAAAGTGGAACGACGCCGAGAAGTGGCTCGCC
GGGAGACACGTCGTGCACTCCAACCCAATCTTCTCCAAGAAGGCCGCCGCCGCAGCAGCGGCCG
TGTCCGGCCGCGTTGCGCCGGAGGCCTCGGCGTCGTCGTCGCCGCCGTCGGTGGCGAGCAGGCA
GCGCCAGCAGAAGAGGCTCCGCGTGTCGTCGGAGGCGGCGGCGGTGTCGATGCGGGACGTGGGG
ACGGAGATGACGCCGATGGCGAGCAAGGAGCAGTCGCGGAGCGGCACGCCCGCCGGCGCCGCCA
CGCCGTCGCTGAGTCCGCTCTGCTCGGTGCCGACGAGCCCGAGGGGCGCCGCGTCCGCGTCGTC
GGCGTCGTCGGAGCGGGAGCTCCAGATCAGGACGCGCCGCGAGATCGCCGCGCTCGGGCTGCAG
CTGGGCAAGATGAACATCGCGTCGTGGGCCAGCAAGGACGACGACGACGAGCTCCCCCGCGCCT
CGCCGGAGAAGAGACCGAGACCGAGACCGAGACCTCACTCCGGCGACGGCGGCGGCGAGGCCAA
GAAGAGGGAGTTCGAGGCGCGCGCCATGGCGTGGCAGGAGACGCACAAGTGCAAGCTCGCGTTG
AG
						
					

						Genomic sequence
						
							ATGGGAGTGATGTCTCCAACCAAGCTGAGGATGAAGCTGCTGGGATCCCATGGCGGCAGCATCG
GCAGGGTTGACGAGGCAAAGAAGTCGCCGCGAGCGTCGCCGGCGAGGCTCGACGCCGACGAGGA
CGACGACGACCACCCCAAGAACAGCCTCCTGCCCCAAGAACTCGACGAAGGTTCGCTTCCTCTT
CGATTCCATTGTGGAGCTTGCAATGGCAATGGTGATGTGTGTTTGATTCCGGTGATTTCTTCGT
CAGATTATCCCAAGGACCAGTCGGATTCTTCTCGCTCGCGCTCCGACGCGAGCCATGGGAGAGC
TGGCAATGGCTACGACAGCGGCGGCTTCGAGTTCTACAGGGAGGAGAGGCCGCCGCCACCGCCG
CCGGCGGCGGTGGCGGTGGTCGGCGGGACGTTCTTCAGGCAGGTGCCGTCAAAGTGGAACGACG
CCGAGAAGTGGCTCGCCGGGAGACACGTCGTGCACTCCAACCCAATCTTCTCCAAGAAGGCCGC
CGCCGCAGCAGCGGCCGTGTCCGGCCGCGTTGCGCCGGAGGCCTCGGCGTCGTCGTCGCCGCCG
TCGGTGGCGAGCAGGCAGCGCCAGCAGAAGAGGCTCCGCGTGTCGTCGGAGGCGGCGGCGGTGT
CGATGCGGGACGTGGGGACGGAGATGACGCCGATGGCGAGCAAGGAGCAGTCGCGGAGCGGCAC
GCCCGCCGGCGCCGCCACGCCGTCGCTGAGTCCGCTCTGCTCGGTGCCGACGAGCCCGAGGGGC
GCCGCGTCCGCGTCGTCGGCGTCGTCGGAGCGGGAGCTCCAGATCAGGACGCGCCGCGAGATCG
CCGCGCTCGGGCTGCAGCTGGGCAAGATGAACATCGCGTCGTGGGCCAGCAAGGACGACGACGA
CGAGCTCCCCCGCGCCTCGCCGGAGAAGAGACCGAGACCGAGACCGAGACCTCACTCCGGCGAC
GGCGGCGGCGAGGCCAAGAAGAGGGAGTTCGAGGCGCGCGCCATGGCGTGGCAGGAGACGCACA
AGTGCAAGCTCGCGTTGAG
						
					

			
		

Sequences
Splice junction sequence	GACCTCACTCCGGCGACGGCGGCGGCGAGGCCAAGAAGAGGGAGTTCGAGGCGCGCGCCATGGC GTGGCAGGAGACGCACAAGTGCAAGCTCGCGTTGAGatgggagtgatgtctccaaccaagctga ggatgaagctgctgggatcccatggcggcagcatcggcagggttgacgaggcaaagaagtcgcc gcgagcgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 962 Transcript exons: 25999419-25999596,25999678-26000461 ATGGGAGTGATGTCTCCAACCAAGCTGAGGATGAAGCTGCTGGGATCCCATGGCGGCAGCATCG GCAGGGTTGACGAGGCAAAGAAGTCGCCGCGAGCGTCGCCGGCGAGGCTCGACGCCGACGAGGA CGACGACGACCACCCCAAGAACAGCCTCCTGCCCCAAGAACTCGACGAAGATTATCCCAAGGAC CAGTCGGATTCTTCTCGCTCGCGCTCCGACGCGAGCCATGGGAGAGCTGGCAATGGCTACGACA GCGGCGGCTTCGAGTTCTACAGGGAGGAGAGGCCGCCGCCACCGCCGCCGGCGGCGGTGGCGGT GGTCGGCGGGACGTTCTTCAGGCAGGTGCCGTCAAAGTGGAACGACGCCGAGAAGTGGCTCGCC GGGAGACACGTCGTGCACTCCAACCCAATCTTCTCCAAGAAGGCCGCCGCCGCAGCAGCGGCCG TGTCCGGCCGCGTTGCGCCGGAGGCCTCGGCGTCGTCGTCGCCGCCGTCGGTGGCGAGCAGGCA GCGCCAGCAGAAGAGGCTCCGCGTGTCGTCGGAGGCGGCGGCGGTGTCGATGCGGGACGTGGGG ACGGAGATGACGCCGATGGCGAGCAAGGAGCAGTCGCGGAGCGGCACGCCCGCCGGCGCCGCCA CGCCGTCGCTGAGTCCGCTCTGCTCGGTGCCGACGAGCCCGAGGGGCGCCGCGTCCGCGTCGTC GGCGTCGTCGGAGCGGGAGCTCCAGATCAGGACGCGCCGCGAGATCGCCGCGCTCGGGCTGCAG CTGGGCAAGATGAACATCGCGTCGTGGGCCAGCAAGGACGACGACGACGAGCTCCCCCGCGCCT CGCCGGAGAAGAGACCGAGACCGAGACCGAGACCTCACTCCGGCGACGGCGGCGGCGAGGCCAA GAAGAGGGAGTTCGAGGCGCGCGCCATGGCGTGGCAGGAGACGCACAAGTGCAAGCTCGCGTTG AG
Genomic sequence	ATGGGAGTGATGTCTCCAACCAAGCTGAGGATGAAGCTGCTGGGATCCCATGGCGGCAGCATCG GCAGGGTTGACGAGGCAAAGAAGTCGCCGCGAGCGTCGCCGGCGAGGCTCGACGCCGACGAGGA CGACGACGACCACCCCAAGAACAGCCTCCTGCCCCAAGAACTCGACGAAGGTTCGCTTCCTCTT CGATTCCATTGTGGAGCTTGCAATGGCAATGGTGATGTGTGTTTGATTCCGGTGATTTCTTCGT CAGATTATCCCAAGGACCAGTCGGATTCTTCTCGCTCGCGCTCCGACGCGAGCCATGGGAGAGC TGGCAATGGCTACGACAGCGGCGGCTTCGAGTTCTACAGGGAGGAGAGGCCGCCGCCACCGCCG CCGGCGGCGGTGGCGGTGGTCGGCGGGACGTTCTTCAGGCAGGTGCCGTCAAAGTGGAACGACG CCGAGAAGTGGCTCGCCGGGAGACACGTCGTGCACTCCAACCCAATCTTCTCCAAGAAGGCCGC CGCCGCAGCAGCGGCCGTGTCCGGCCGCGTTGCGCCGGAGGCCTCGGCGTCGTCGTCGCCGCCG TCGGTGGCGAGCAGGCAGCGCCAGCAGAAGAGGCTCCGCGTGTCGTCGGAGGCGGCGGCGGTGT CGATGCGGGACGTGGGGACGGAGATGACGCCGATGGCGAGCAAGGAGCAGTCGCGGAGCGGCAC GCCCGCCGGCGCCGCCACGCCGTCGCTGAGTCCGCTCTGCTCGGTGCCGACGAGCCCGAGGGGC GCCGCGTCCGCGTCGTCGGCGTCGTCGGAGCGGGAGCTCCAGATCAGGACGCGCCGCGAGATCG CCGCGCTCGGGCTGCAGCTGGGCAAGATGAACATCGCGTCGTGGGCCAGCAAGGACGACGACGA CGAGCTCCCCCGCGCCTCGCCGGAGAAGAGACCGAGACCGAGACCGAGACCTCACTCCGGCGAC GGCGGCGGCGAGGCCAAGAAGAGGGAGTTCGAGGCGCGCGCCATGGCGTGGCAGGAGACGCACA AGTGCAAGCTCGCGTTGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.156475439

Conservation Information
Conserved circRNAs	NA
PMCS	0.156475439

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							25999420-25999425(+)
25999482-26000232(-)
25999732-26000368(-)
						
						Potential amino acid sequence
						
							MGVMSPTKLRMKLLGSHGGSIGRVDEAKKSPRASPARLDADEDDDDHPKNSLLPQELDEDYPKD
QSDSSRSRSDASHGRAGNGYDSGGFEFYREERPPPPPPAAVAVVGGTFFRQVPSKWNDAEKWLA
GRHVVHSNPIFSKKAAAAAAAVSGRVAPEASASSSPPSVASRQRQQKRLRVSSEAAAVSMRDVG
TEMTPMASKEQSRSGTPAGAATPSLSPLCSVPTSPRGAASASSASSERELQIRTRREIAALGLQ
LGKMNIASWASKDDDDELPRASPEKRPRPRPRPHSGDGGGEAKKREFEARAMAWQETHKCKLAL
RWE*(+)
MLPPWDPSSFILSLVGDITPISTRACTCASPATPWRAPRTPSSWPRRRRRRSEVSVSVSVSSPA
RRGGARRRRPCWPTTRCSSCPAAARARRSRGAS*(-)
MARVGARARRIRLVLGIIFVEFLGQEAVLGVVVVVLVGVEPRRRRSRRLLCLVNPADAAAMGSQ
QLHPQLGWRHHSHLNASLHLCVSCHAMARASNSLFLASPPPSPE*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	25999420-25999425(+) 25999482-26000232(-) 25999732-26000368(-)
Potential amino acid sequence	MGVMSPTKLRMKLLGSHGGSIGRVDEAKKSPRASPARLDADEDDDDHPKNSLLPQELDEDYPKD QSDSSRSRSDASHGRAGNGYDSGGFEFYREERPPPPPPAAVAVVGGTFFRQVPSKWNDAEKWLA GRHVVHSNPIFSKKAAAAAAAVSGRVAPEASASSSPPSVASRQRQQKRLRVSSEAAAVSMRDVG TEMTPMASKEQSRSGTPAGAATPSLSPLCSVPTSPRGAASASSASSERELQIRTRREIAALGLQ LGKMNIASWASKDDDDELPRASPEKRPRPRPRPHSGDGGGEAKKREFEARAMAWQETHKCKLAL RWE(+) MLPPWDPSSFILSLVGDITPISTRACTCASPATPWRAPRTPSSWPRRRRRRSEVSVSVSVSSPA RRGGARRRRPCWPTTRCSSCPAAARARRSRGAS(-) MARVGARARRIRLVLGIIFVEFLGQEAVLGVVVVVLVGVEPRRRRSRRLLCLVNPADAAAMGSQ QLHPQLGWRHHSHLNASLHLCVSCHAMARASNSLFLASPPPSPE*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os12g0613600_circ_g.1

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