Plant Circular RNA Database

Detail information of orai.008G215300_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.008G215300_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000866
					
						Alias
						Chr08:50218746|50221255
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr08: 50218746-50221255  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.008G215300						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						24
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.008G215300.3:5
Gorai.008G215300.2:5
Gorai.008G215300.1:5
Gorai.008G215300.5:5
Gorai.008G215300.4:5

General Information
CircRNA Name	orai.008G215300_circ_g.1
ID in PlantcircBase	gra_circ_000866
Alias	Chr08:50218746\|50221255
Organism	Gossypium raimondii
Position	chrChr08: 50218746-50221255 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.008G215300
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	24
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.008G215300.3:5 Gorai.008G215300.2:5 Gorai.008G215300.1:5 Gorai.008G215300.5:5 Gorai.008G215300.4:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGCCTAATCAGACTGTTGTATGGTCAAAAATGGAGTGATGGAGAAGCTTCCACAGCCCTTCGTT
ACTATTGTCTTTACATCATTGTTTTGGCTATGAATGgagaagggaattgtctttgcgttgtctc
aagcagcatatggagcctgcatatttctgggctattggagctattttcttctttttcgtgcata
taggagtt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 568
Transcript exons: 50218746-50218864,50219305-50219468,50219651-50219725,50
220611-50220682,50221118-50221255
GAGAAGGGAATTGTCTTTGCGTTGTCTCAAGCAGCATATGGAGCCTGCATATTTCTGGGCTATT
GGAGCTATTTTCTTCTTTTTCGTGCATATAGGAGTTCTGATCTTATTCCTTTCAGACTAGGACA
TATGATGAATTTTGATAAGCAGCTCTCAAACATGTGTATGTTGTTTACCCTTCAATCCTTTCGA
AAGTTGATCCTTCAAGAAGGAGAAAAGATCGTCCTTGTATGGTTGGATACACCATATAACCAAG
CCGTATATGGACTCGTGGACAAACTAGGGAGCTTGGTGGTCAGGTTGGTGTTTCTTCCTTTTGA
AGAAAGTTCATATTCTACATTTGCCAGGTCTGCATCAGGACAGTCTCCAAATAAAAGCAAGAAC
TTAGGAAGGCGCCTTACTGAGGCCATGAAGCTTGTTTTACTAATCGGTCTCTTATTTTTGGCTT
TTGGCCCAAGCTACTCCTATTGCCTAATCAGACTGTTGTATGGTCAAAAATGGAGTGATGGAGA
AGCTTCCACAGCCCTTCGTTACTATTGTCTTTACATCATTGTTTTGGCTATGAATG
						
					

						Genomic sequence
						
							GAGAAGGGAATTGTCTTTGCGTTGTCTCAAGCAGCATATGGAGCCTGCATATTTCTGGGCTATT
GGAGCTATTTTCTTCTTTTTCGTGCATATAGGAGTTCTGATCTTATTCCTTTCAGGTACTGGTC
CTTTTATGCTTGACCCCATTCTTTTGGGGTAATATGTATGCTTGAATTGTTAGTCCTCCTTTCC
CTTATTTGTGGATAAAATTTAAAGAAGACTTGAATTAATGGTAAATAAGTATACCCATCCACTT
TTCTCGACATTGACCTGATGGAAATTTAGGTTTCCACATATAAATTAACATCCTGATGCTACCT
TAGAGCTGTCGTCGTAACGGCTTTATCCGAGTACCATGAGAATCCTATCCATGTATTGGACTTC
AACCCTGTTTGTCTGATTCTATACATATATCATTGATTCTCCTAAATACATCTCTTTTATTTTA
TTTTTTTATGTCTAAAGAACACATTTATTTCCTGAATTGTTTGGTAGCTACTTGCTCTGCCTTT
TAATTACTTTCTCCAGATTATCCATAAATAACGCACAACTTTTGCAGACTAGGACATATGATGA
ATTTTGATAAGCAGCTCTCAAACATGTGTATGTTGTTTACCCTTCAATCCTTTCGAAAGTTGAT
CCTTCAAGAAGGAGAAAAGATCGTCCTTGTATGGTTGGATACACCATATAACCAAGCCGTATAT
GGACTCGTGGACAAACTAGGTGAGTAATCAGCACACTTGCTTCATACTTTATAAAGTTTTAGTT
GGTAGGTTTCTAATGCTGGACTGTTTTGTTGGATATAATGCAATGCCTCTTATAATTTTCCACT
ACGATCGAGAAGATGCGATTATTTTTTCAAGGGCTAATCTAAATCATTCTTTTTTTCTTTTTTT
TTAATTTAGGGAGCTTGGTGGTCAGGTTGGTGTTTCTTCCTTTTGAAGAAAGTTCATATTCTAC
ATTTGCCAGGTCTGCATCAGGTTTATTACTGTCCTTTTCTTCAGTTGATTTTGTGAGGTGTTTA
AACTTGTTTCTTAGGATTCACATATCTGGTTAACATTAATTTTTGACTACTTTTCTTTGTATTT
GCTGACCCAACTCTCAGAATTGTGTCCATATTTTTGTTTTACATCAAGTATAGCTCCCATATGA
ATAGGATGAAGTCAGATTGCTATAGGATGTCCTTCAATTAAATTAAACAGGCACTTTTTCTTGA
AGAGAGAGAGATAAAGGATAGGTGGATGTTAGGCGGTGGAAAAGAAAGTAGCACTCTCCATTGT
CCATAAATTACAAGAAAATTTCCTTTTTGTTCAGCATTTGTACAGTGAATATGATTGCATGTGT
TTGGGAGCTTGGATTAATTTAATGCTGCTGGCACAATATACGACAGATGTATTCTTCCCTGTAG
CTTGGTGGTTTTTGTCTATGTTGTGCATGAAACGAACATCATATTATATGTTTGAATCACATTT
TATGTTACTAATCCAATTGTTTTCTGGGAGTTATTTCATGGGAGTTAGTGTGAAGGTGGTAATT
TATGGTGAATCTTCAGCCTGTTAATCTAGCTTCCCCTATCCCTGTTTTTTTTATATTCATATGT
TGCATCCTAAGGCCTAAACTAATTAGTGATCTTTTTATTATTATTATTATTGTTATTATTGTGA
AAAATGTTTATCCACGCACTAGAACTCAGCTTCCTTTTCCTTCAGACAACCTGCTTCTTGCTTC
CATGAAGATTTAACTTTCTAAATGAGATATTTTGTTAATTAGTTGCCTTTGAATGTTTATGCAC
ACAGATGCTCATTCTCCATAATTAAGATGGTGCTTCCTAACTTGTATCATATTGATAAAATATC
TCTGGACAGGACAGTCTCCAAATAAAAGCAAGAACTTAGGAAGGCGCCTTACTGAGGCCATGAA
GCTTGTTTTACTAATCGGTATGTTCTAAAGTTTTTTGTACTTCTTGATGTTTCCCTCTTTTCCT
AGGATCAGTTTTCTAGGCATTGCCAACAATATTGTAATATAATGAAATTCTTAATTGGTTCTGG
AAGTAATATAGGAATAATATCATAATTTTGGGACTTACAATAGTAATACAATAGTTCTAAAATT
AGAGACTATGATTTCTATTGGCTGATTTAGAATGCTTCTTAGTTTGAGTGATGATAGAGTACAG
TACATTTTAATCTAAGGAATTAATTTTGTTCTAATGATAATCTCTGTTTTGTTTATTTTTATAC
TAATTGGCACAACCTGCTTCCTTTCGATCAGTTTGATATGCCTTGGCATACCACTATGTTCTTG
AAAATCTTTTTTCTTTTAAAACATAATGCTCTTTTTATGTTTTCCTCAAGGTGCTACTTATTTT
GTAGGTCTCTTATTTTTGGCTTTTGGCCCAAGCTACTCCTATTGCCTAATCAGACTGTTGTATG
GTCAAAAATGGAGTGATGGAGAAGCTTCCACAGCCCTTCGTTACTATTGTCTTTACATCATTGT
TTTGGCTATGAATG
						
					

			
		

Sequences
Splice junction sequence	TGCCTAATCAGACTGTTGTATGGTCAAAAATGGAGTGATGGAGAAGCTTCCACAGCCCTTCGTT ACTATTGTCTTTACATCATTGTTTTGGCTATGAATGgagaagggaattgtctttgcgttgtctc aagcagcatatggagcctgcatatttctgggctattggagctattttcttctttttcgtgcata taggagtt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 568 Transcript exons: 50218746-50218864,50219305-50219468,50219651-50219725,50 220611-50220682,50221118-50221255 GAGAAGGGAATTGTCTTTGCGTTGTCTCAAGCAGCATATGGAGCCTGCATATTTCTGGGCTATT GGAGCTATTTTCTTCTTTTTCGTGCATATAGGAGTTCTGATCTTATTCCTTTCAGACTAGGACA TATGATGAATTTTGATAAGCAGCTCTCAAACATGTGTATGTTGTTTACCCTTCAATCCTTTCGA AAGTTGATCCTTCAAGAAGGAGAAAAGATCGTCCTTGTATGGTTGGATACACCATATAACCAAG CCGTATATGGACTCGTGGACAAACTAGGGAGCTTGGTGGTCAGGTTGGTGTTTCTTCCTTTTGA AGAAAGTTCATATTCTACATTTGCCAGGTCTGCATCAGGACAGTCTCCAAATAAAAGCAAGAAC TTAGGAAGGCGCCTTACTGAGGCCATGAAGCTTGTTTTACTAATCGGTCTCTTATTTTTGGCTT TTGGCCCAAGCTACTCCTATTGCCTAATCAGACTGTTGTATGGTCAAAAATGGAGTGATGGAGA AGCTTCCACAGCCCTTCGTTACTATTGTCTTTACATCATTGTTTTGGCTATGAATG
Genomic sequence	GAGAAGGGAATTGTCTTTGCGTTGTCTCAAGCAGCATATGGAGCCTGCATATTTCTGGGCTATT GGAGCTATTTTCTTCTTTTTCGTGCATATAGGAGTTCTGATCTTATTCCTTTCAGGTACTGGTC CTTTTATGCTTGACCCCATTCTTTTGGGGTAATATGTATGCTTGAATTGTTAGTCCTCCTTTCC CTTATTTGTGGATAAAATTTAAAGAAGACTTGAATTAATGGTAAATAAGTATACCCATCCACTT TTCTCGACATTGACCTGATGGAAATTTAGGTTTCCACATATAAATTAACATCCTGATGCTACCT TAGAGCTGTCGTCGTAACGGCTTTATCCGAGTACCATGAGAATCCTATCCATGTATTGGACTTC AACCCTGTTTGTCTGATTCTATACATATATCATTGATTCTCCTAAATACATCTCTTTTATTTTA TTTTTTTATGTCTAAAGAACACATTTATTTCCTGAATTGTTTGGTAGCTACTTGCTCTGCCTTT TAATTACTTTCTCCAGATTATCCATAAATAACGCACAACTTTTGCAGACTAGGACATATGATGA ATTTTGATAAGCAGCTCTCAAACATGTGTATGTTGTTTACCCTTCAATCCTTTCGAAAGTTGAT CCTTCAAGAAGGAGAAAAGATCGTCCTTGTATGGTTGGATACACCATATAACCAAGCCGTATAT GGACTCGTGGACAAACTAGGTGAGTAATCAGCACACTTGCTTCATACTTTATAAAGTTTTAGTT GGTAGGTTTCTAATGCTGGACTGTTTTGTTGGATATAATGCAATGCCTCTTATAATTTTCCACT ACGATCGAGAAGATGCGATTATTTTTTCAAGGGCTAATCTAAATCATTCTTTTTTTCTTTTTTT TTAATTTAGGGAGCTTGGTGGTCAGGTTGGTGTTTCTTCCTTTTGAAGAAAGTTCATATTCTAC ATTTGCCAGGTCTGCATCAGGTTTATTACTGTCCTTTTCTTCAGTTGATTTTGTGAGGTGTTTA AACTTGTTTCTTAGGATTCACATATCTGGTTAACATTAATTTTTGACTACTTTTCTTTGTATTT GCTGACCCAACTCTCAGAATTGTGTCCATATTTTTGTTTTACATCAAGTATAGCTCCCATATGA ATAGGATGAAGTCAGATTGCTATAGGATGTCCTTCAATTAAATTAAACAGGCACTTTTTCTTGA AGAGAGAGAGATAAAGGATAGGTGGATGTTAGGCGGTGGAAAAGAAAGTAGCACTCTCCATTGT CCATAAATTACAAGAAAATTTCCTTTTTGTTCAGCATTTGTACAGTGAATATGATTGCATGTGT TTGGGAGCTTGGATTAATTTAATGCTGCTGGCACAATATACGACAGATGTATTCTTCCCTGTAG CTTGGTGGTTTTTGTCTATGTTGTGCATGAAACGAACATCATATTATATGTTTGAATCACATTT TATGTTACTAATCCAATTGTTTTCTGGGAGTTATTTCATGGGAGTTAGTGTGAAGGTGGTAATT TATGGTGAATCTTCAGCCTGTTAATCTAGCTTCCCCTATCCCTGTTTTTTTTATATTCATATGT TGCATCCTAAGGCCTAAACTAATTAGTGATCTTTTTATTATTATTATTATTGTTATTATTGTGA AAAATGTTTATCCACGCACTAGAACTCAGCTTCCTTTTCCTTCAGACAACCTGCTTCTTGCTTC CATGAAGATTTAACTTTCTAAATGAGATATTTTGTTAATTAGTTGCCTTTGAATGTTTATGCAC ACAGATGCTCATTCTCCATAATTAAGATGGTGCTTCCTAACTTGTATCATATTGATAAAATATC TCTGGACAGGACAGTCTCCAAATAAAAGCAAGAACTTAGGAAGGCGCCTTACTGAGGCCATGAA GCTTGTTTTACTAATCGGTATGTTCTAAAGTTTTTTGTACTTCTTGATGTTTCCCTCTTTTCCT AGGATCAGTTTTCTAGGCATTGCCAACAATATTGTAATATAATGAAATTCTTAATTGGTTCTGG AAGTAATATAGGAATAATATCATAATTTTGGGACTTACAATAGTAATACAATAGTTCTAAAATT AGAGACTATGATTTCTATTGGCTGATTTAGAATGCTTCTTAGTTTGAGTGATGATAGAGTACAG TACATTTTAATCTAAGGAATTAATTTTGTTCTAATGATAATCTCTGTTTTGTTTATTTTTATAC TAATTGGCACAACCTGCTTCCTTTCGATCAGTTTGATATGCCTTGGCATACCACTATGTTCTTG AAAATCTTTTTTCTTTTAAAACATAATGCTCTTTTTATGTTTTCCTCAAGGTGCTACTTATTTT GTAGGTCTCTTATTTTTGGCTTTTGGCCCAAGCTACTCCTATTGCCTAATCAGACTGTTGTATG GTCAAAAATGGAGTGATGGAGAAGCTTCCACAGCCCTTCGTTACTATTGTCTTTACATCATTGT TTTGGCTATGAATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							ghi_circ_000419* 						
					
						PMCS
						0.859639598

Conservation Information
Conserved circRNAs	ghi_circ_000419*
PMCS	0.859639598

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							50219315-50218837(+)
50221253-50218837(+)
50218837-50221232(-)
						
						Potential amino acid sequence
						
							MMNFDKQLSNMCMLFTLQSFRKLILQEGEKIVLVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFE
ESSYSTFARSASGQSPNKSKNLGRRLTEAMKLVLLIGLLFLAFGPSYSYCLIRLLYGQKWSDGE
ASTALRYYCLYIIVLAMNGEGNCLCVVSSSIWSLHISGLLELFSSFSCI*(+)
MEKGIVFALSQAAYGACIFLGYWSYFLLFRAYRSSDLIPFRLGHMMNFDKQLSNMCMLFTLQSF
RKLILQEGEKIVLVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYSTFARSASGQSPNKSK
NLGRRLTEAMKLVLLIGLLFLAFGPSYSYCLIRLLYGQKWSDGEASTALRYYCLYIIVLAMNGE
GNCLCVVSSSIWSLHISGLLELFSSFSCI*(+)
MHEKEENSSNSPEICRLHMLLETTQRQFPSPFIAKTMM*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	50219315-50218837(+) 50221253-50218837(+) 50218837-50221232(-)
Potential amino acid sequence	MMNFDKQLSNMCMLFTLQSFRKLILQEGEKIVLVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFE ESSYSTFARSASGQSPNKSKNLGRRLTEAMKLVLLIGLLFLAFGPSYSYCLIRLLYGQKWSDGE ASTALRYYCLYIIVLAMNGEGNCLCVVSSSIWSLHISGLLELFSSFSCI(+) MEKGIVFALSQAAYGACIFLGYWSYFLLFRAYRSSDLIPFRLGHMMNFDKQLSNMCMLFTLQSF RKLILQEGEKIVLVWLDTPYNQAVYGLVDKLGSLVVRLVFLPFEESSYSTFARSASGQSPNKSK NLGRRLTEAMKLVLLIGLLFLAFGPSYSYCLIRLLYGQKWSDGEASTALRYYCLYIIVLAMNGE GNCLCVVSSSIWSLHISGLLELFSSFSCI(+) MHEKEENSSNSPEICRLHMLLETTQRQFPSPFIAKTMM*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.008G215300_circ_g.1

Address

Any questions?

Supported by