Plant Circular RNA Database

Detail information of AT5G15020_circ_g.12

			
						 General Information
					
						CircRNA Name
						AT5G15020_circ_g.12
					
						ID in PlantcircBase
						ath_circ_038356
					
						Alias
						At_ciR288, Ath_circ_FC3310, Ath_circ_FC3311, AT5G15020_C2
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 4865208-4865576  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT5G15020						
					
						Parent gene annotation
						
							Paired amphipathic helix protein Sin3-like 2
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT5G15020_circ_g.5 AT5G15020_circ_g.6 AT5G15020_circ_g.7 AT5G15020_circ_g.8 AT5G15020_circ_g.9 AT5G15020_circ_g.10 AT5G15020_circ_g.11 						
					
						Support reads
						10/1
					
						Tissues
						leaf/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G15020.1:2
AT5G15020.2:2

General Information
CircRNA Name	AT5G15020_circ_g.12
ID in PlantcircBase	ath_circ_038356
Alias	At_ciR288, Ath_circ_FC3310, Ath_circ_FC3311, AT5G15020_C2
Organism	Arabidpsis thaliana
Position	chr5: 4865208-4865576 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	CIRCexplorer, find_circ, CIRI-full, CIRI2
Parent gene	AT5G15020
Parent gene annotation	Paired amphipathic helix protein Sin3-like 2
Parent gene strand	-
Alternative splicing	AT5G15020_circ_g.5 AT5G15020_circ_g.6 AT5G15020_circ_g.7 AT5G15020_circ_g.8 AT5G15020_circ_g.9 AT5G15020_circ_g.10 AT5G15020_circ_g.11
Support reads	10/1
Tissues	leaf/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G15020.1:2 AT5G15020.2:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAAAAGGGAAACTCACGATTCGCCACGAGAAGAGCCCAAAGGACGTTTAAATTGAGACCCGGTT
GCGTAAATATCATCCCTAATTCGCTTCATTGCTGCTttttgtgccttaaagtctttcataacct
caaggaacatatcatatttgtctcgctgatcttgaaacatctcctttacttcctttaagtaggt
caaagcat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 229
Transcript exons: 4865208-4865356,4865497-4865576
TTTTGTGCCTTAAAGTCTTTCATAACCTCAAGGAACATATCATATTTGTCTCGCTGATCTTGAA
ACATCTCCTTTACTTCCTTTAAGTAGGTCAAAGCATCATCGGTAGTCAATTTCTGAGTGTTGAT
TCCCCCTTCACCAATGCTCCCCGCCACGAGAAGAGCCCAAAGGACGTTTAAATTGAGACCCGGT
TGCGTAAATATCATCCCTAATTCGCTTCATTGCTGCT
Transcript length: 261
Transcript exons: 4865208-4865384,4865493-4865576
TTTTGTGCCTTAAAGTCTTTCATAACCTCAAGGAACATATCATATTTGTCTCGCTGATCTTGAA
ACATCTCCTTTACTTCCTTTAAGTAGGTCAAAGCATCATCGGTAGTCAATTTCTGAGTGTTGAT
TCCCCCTTCACCAATGCTCCCTCCTCCAGTGATTGGAGATTGCTCATATGATTCGCCACGAGAA
GAGCCCAAAGGACGTTTAAATTGAGACCCGGTTGCGTAAATATCATCCCTAATTCGCTTCATTG
CTGCT
						
						Genomic sequence
						
							TTTTGTGCCTTAAAGTCTTTCATAACCTCAAGGAACATATCATATTTGTCTCGCTGATCTTGAA
ACATCTCCTTTACTTCCTTTAAGTAGGTCAAAGCATCATCGGTAGTCAATTTCTGAGTGTTGAT
TCCCCCTTCACCAATGCTCCCTCCTCCAGTGATTGGAGATTGCTCATATCTGCACCAAACAAAC
TCTAATCACTTAGTAACTTTCATGCAAACAAGCTATAATGTCTCATCGCCAGCAATTTTAAATT
TTCGAAGTCTGACGAAAAGGGAAACTCACGATTCGCCACGAGAAGAGCCCAAAGGACGTTTAAA
TTGAGACCCGGTTGCGTAAATATCATCCCTAATTCGCTTCATTGCTGCT

Sequences
Splice junction sequence	GAAAAGGGAAACTCACGATTCGCCACGAGAAGAGCCCAAAGGACGTTTAAATTGAGACCCGGTT GCGTAAATATCATCCCTAATTCGCTTCATTGCTGCTttttgtgccttaaagtctttcataacct caaggaacatatcatatttgtctcgctgatcttgaaacatctcctttacttcctttaagtaggt caaagcat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 229 Transcript exons: 4865208-4865356,4865497-4865576 TTTTGTGCCTTAAAGTCTTTCATAACCTCAAGGAACATATCATATTTGTCTCGCTGATCTTGAA ACATCTCCTTTACTTCCTTTAAGTAGGTCAAAGCATCATCGGTAGTCAATTTCTGAGTGTTGAT TCCCCCTTCACCAATGCTCCCCGCCACGAGAAGAGCCCAAAGGACGTTTAAATTGAGACCCGGT TGCGTAAATATCATCCCTAATTCGCTTCATTGCTGCT Transcript length: 261 Transcript exons: 4865208-4865384,4865493-4865576 TTTTGTGCCTTAAAGTCTTTCATAACCTCAAGGAACATATCATATTTGTCTCGCTGATCTTGAA ACATCTCCTTTACTTCCTTTAAGTAGGTCAAAGCATCATCGGTAGTCAATTTCTGAGTGTTGAT TCCCCCTTCACCAATGCTCCCTCCTCCAGTGATTGGAGATTGCTCATATGATTCGCCACGAGAA GAGCCCAAAGGACGTTTAAATTGAGACCCGGTTGCGTAAATATCATCCCTAATTCGCTTCATTG CTGCT
Genomic sequence	TTTTGTGCCTTAAAGTCTTTCATAACCTCAAGGAACATATCATATTTGTCTCGCTGATCTTGAA ACATCTCCTTTACTTCCTTTAAGTAGGTCAAAGCATCATCGGTAGTCAATTTCTGAGTGTTGAT TCCCCCTTCACCAATGCTCCCTCCTCCAGTGATTGGAGATTGCTCATATCTGCACCAAACAAAC TCTAATCACTTAGTAACTTTCATGCAAACAAGCTATAATGTCTCATCGCCAGCAATTTTAAATT TTCGAAGTCTGACGAAAAGGGAAACTCACGATTCGCCACGAGAAGAGCCCAAAGGACGTTTAAA TTGAGACCCGGTTGCGTAAATATCATCCCTAATTCGCTTCATTGCTGCT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.618182487

Conservation Information
Conserved circRNAs	NA
PMCS	0.618182487

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4865570-4865210(-)
						
						Potential amino acid sequence
						
							MKRIRDDIYATGSQFKRPLGSSRGESYEQSPITGGGSIGEGGINTQKLTTDDALTYLKEVKEMF
QDQRDKYDMFLEVMKDFKAQKAAMKRIRDDIYATGSQFKRPLGSSRGESYEQSPITGGGSIGEG
GINTQKLTTDDALTYLKEVKEMFQDQRDKYDMFLEVMKDFKAQKAAMKRIRDDIYATGSQFKRP
LGSSRGESYEQSPITGGGSIGEGGINTQKLTTDDALTYLKEVKEMFQDQRDKYDMFLEVMKDFK
AQKAAMKRIRDDIYATGSQFKRPLGSSRGESYEQSPITGGGSIGEGGINTQKLTTDDALTYLKE
VKEMFQDQRDKYDMFLEVMKDFKAQ(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	4865570-4865210(-)
Potential amino acid sequence	MKRIRDDIYATGSQFKRPLGSSRGESYEQSPITGGGSIGEGGINTQKLTTDDALTYLKEVKEMF QDQRDKYDMFLEVMKDFKAQKAAMKRIRDDIYATGSQFKRPLGSSRGESYEQSPITGGGSIGEG GINTQKLTTDDALTYLKEVKEMFQDQRDKYDMFLEVMKDFKAQKAAMKRIRDDIYATGSQFKRP LGSSRGESYEQSPITGGGSIGEGGINTQKLTTDDALTYLKEVKEMFQDQRDKYDMFLEVMKDFK AQKAAMKRIRDDIYATGSQFKRPLGSSRGESYEQSPITGGGSIGEGGINTQKLTTDDALTYLKE VKEMFQDQRDKYDMFLEVMKDFKAQ(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Chen et al., 2017a;Zhang et al., 2019

circRNA

Detail information of AT5G15020_circ_g.12

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