Plant Circular RNA Database

Detail information of Os01g0709500_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os01g0709500_circ_g.1
					
						ID in PlantcircBase
						osa_circ_003582
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 29487907-29488564  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os01g0709500						
					
						Parent gene annotation
						
							Conserved hypothetical protein. (Os01t0709500-01);Conserved hypo
thetical protein. (Os01t0709500-02);Similar to Serine/threonine-
protein kinase PBS1 (EC 2.7.1.37) (AvrPphB susceptible protein 1
). (Os01t0709500-03);Similar to Protein kinase. (Os01t0709500-04
)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os01g0709500_circ_g.2 Os01g0709500_circ_g.3 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0709500-03:2
Os01t0709500-01:2
Os01t0709500-02:2

General Information
CircRNA Name	Os01g0709500_circ_g.1
ID in PlantcircBase	osa_circ_003582
Alias	NA
Organism	Oryza sativa
Position	chr1: 29487907-29488564 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os01g0709500
Parent gene annotation	Conserved hypothetical protein. (Os01t0709500-01);Conserved hypo thetical protein. (Os01t0709500-02);Similar to Serine/threonine- protein kinase PBS1 (EC 2.7.1.37) (AvrPphB susceptible protein 1 ). (Os01t0709500-03);Similar to Protein kinase. (Os01t0709500-04 )
Parent gene strand	-
Alternative splicing	Os01g0709500_circ_g.2 Os01g0709500_circ_g.3
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0709500-03:2 Os01t0709500-01:2 Os01t0709500-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAATGATGATGCCTTTCTTTTGAATGTGCTGCAATTGGAACTCCATGGGCCTTCCCATGGTTGG
TGGGATGGATTATTGATGCTGGAGACACTGCTGGTCctggagaatttgaaggcgagtgatattc
atggggatgacgtggtgcatacgcaggatttcctcgtgcatttccttcagatggaggcaagcct
acaggtgc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 581
Transcript exons: 29487907-29488280,29488358-29488564
CTGGAGAATTTGAAGGCGAGTGATATTCATGGGGATGACGTGGTGCATACGCAGGATTTCCTCG
TGCATTTCCTTCAGATGGAGGCAAGCCTACAGGTGCAGGTGCAGGACTATGGTGTAAGCCAGAA
CGTCCATGTTTCGTCGCAGAAGCATTATCAGCTTTATGTGGAGTAGGGCTTATGACAGGAAAGG
ATCCTGCAGCAAACATTGCCTTAGTGAGATGAGTACTTTTTTTTTTTTTACTGTCAGCATACCT
TTGTGGGGCCTATGATGTGAAGGTGGTAAATTGCTGGAATGTTGCTTTGGAGGTGCAGCAACTG
GAATGCCATGTTTTCTATGGATACTGGGAGAATTTGAAGGAGAATAGGCAGGTCCTTTTACACG
GGTATTATTTACAGGCAAAGAATGGTGATGCCTTCCCTTTGGAGGTGCCACAACTGGACCTGCA
TGGGTATTTCCATGTGTATTATTTACAAGCATGGAATGATGATGCCTTTCTTTTGAATGTGCTG
CAATTGGAACTCCATGGGCCTTCCCATGGTTGGTGGGATGGATTATTGATGCTGGAGACACTGC
TGGTC
						
						Genomic sequence
						
							CTGGAGAATTTGAAGGCGAGTGATATTCATGGGGATGACGTGGTGCATACGCAGGATTTCCTCG
TGCATTTCCTTCAGATGGAGGCAAGCCTACAGGTGCAGGTGCAGGACTATGGTGTAAGCCAGAA
CGTCCATGTTTCGTCGCAGAAGCATTATCAGCTTTATGTGGAGTAGGGCTTATGACAGGAAAGG
ATCCTGCAGCAAACATTGCCTTAGTGAGATGAGTACTTTTTTTTTTTTTACTGTCAGCATACCT
TTGTGGGGCCTATGATGTGAAGGTGGTAAATTGCTGGAATGTTGCTTTGGAGGTGCAGCAACTG
GAATGCCATGTTTTCTATGGATACTGGGAGAATTTGAAGGAGAATAGGCAGGTCCTGCTCGATA
CAACTTCATGAATCATTTTATTACCAGCAATAGGTTCATTATGGTAGTTGTGCAGTCGGTGGAA
TACCTTTTACACGGGTATTATTTACAGGCAAAGAATGGTGATGCCTTCCCTTTGGAGGTGCCAC
AACTGGACCTGCATGGGTATTTCCATGTGTATTATTTACAAGCATGGAATGATGATGCCTTTCT
TTTGAATGTGCTGCAATTGGAACTCCATGGGCCTTCCCATGGTTGGTGGGATGGATTATTGATG
CTGGAGACACTGCTGGTC

Sequences
Splice junction sequence	GAATGATGATGCCTTTCTTTTGAATGTGCTGCAATTGGAACTCCATGGGCCTTCCCATGGTTGG TGGGATGGATTATTGATGCTGGAGACACTGCTGGTCctggagaatttgaaggcgagtgatattc atggggatgacgtggtgcatacgcaggatttcctcgtgcatttccttcagatggaggcaagcct acaggtgc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 581 Transcript exons: 29487907-29488280,29488358-29488564 CTGGAGAATTTGAAGGCGAGTGATATTCATGGGGATGACGTGGTGCATACGCAGGATTTCCTCG TGCATTTCCTTCAGATGGAGGCAAGCCTACAGGTGCAGGTGCAGGACTATGGTGTAAGCCAGAA CGTCCATGTTTCGTCGCAGAAGCATTATCAGCTTTATGTGGAGTAGGGCTTATGACAGGAAAGG ATCCTGCAGCAAACATTGCCTTAGTGAGATGAGTACTTTTTTTTTTTTTACTGTCAGCATACCT TTGTGGGGCCTATGATGTGAAGGTGGTAAATTGCTGGAATGTTGCTTTGGAGGTGCAGCAACTG GAATGCCATGTTTTCTATGGATACTGGGAGAATTTGAAGGAGAATAGGCAGGTCCTTTTACACG GGTATTATTTACAGGCAAAGAATGGTGATGCCTTCCCTTTGGAGGTGCCACAACTGGACCTGCA TGGGTATTTCCATGTGTATTATTTACAAGCATGGAATGATGATGCCTTTCTTTTGAATGTGCTG CAATTGGAACTCCATGGGCCTTCCCATGGTTGGTGGGATGGATTATTGATGCTGGAGACACTGC TGGTC
Genomic sequence	CTGGAGAATTTGAAGGCGAGTGATATTCATGGGGATGACGTGGTGCATACGCAGGATTTCCTCG TGCATTTCCTTCAGATGGAGGCAAGCCTACAGGTGCAGGTGCAGGACTATGGTGTAAGCCAGAA CGTCCATGTTTCGTCGCAGAAGCATTATCAGCTTTATGTGGAGTAGGGCTTATGACAGGAAAGG ATCCTGCAGCAAACATTGCCTTAGTGAGATGAGTACTTTTTTTTTTTTTACTGTCAGCATACCT TTGTGGGGCCTATGATGTGAAGGTGGTAAATTGCTGGAATGTTGCTTTGGAGGTGCAGCAACTG GAATGCCATGTTTTCTATGGATACTGGGAGAATTTGAAGGAGAATAGGCAGGTCCTGCTCGATA CAACTTCATGAATCATTTTATTACCAGCAATAGGTTCATTATGGTAGTTGTGCAGTCGGTGGAA TACCTTTTACACGGGTATTATTTACAGGCAAAGAATGGTGATGCCTTCCCTTTGGAGGTGCCAC AACTGGACCTGCATGGGTATTTCCATGTGTATTATTTACAAGCATGGAATGATGATGCCTTTCT TTTGAATGTGCTGCAATTGGAACTCCATGGGCCTTCCCATGGTTGGTGGGATGGATTATTGATG CTGGAGACACTGCTGGTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.334041502

Conservation Information
Conserved circRNAs	NA
PMCS	0.334041502

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							29488545-29488077(+)
29488116-29488086(-)
29487977-29488540(-)
						
						Potential amino acid sequence
						
							MLETLLVLENLKASDIHGDDVVHTQDFLVHFLQMEASLQVQVQDYGVSQNVHVSSQKHYQLYVE
*(+)
MFAAGSFPVISPTPHKADNASATKHGRSGLHHSPAPAPVGLPPSEGNARGNPAYAPRHPHEYHS
PSNSPGPAVSPASIIHPTNHGKAHGVPIAAHSKERHHHSMLVNNTHGNTHAGPVVAPPKGRHHH
SLPVNNTRVKGPAYSPSNSPSIHRKHGIPVAAPPKQHSSNLPPSHHRPHKGMLTVKKKKVLISL
RQCLLQDPFLS*(-)
MHEEILRMHHVIPMNITRLQILQDQQCLQHQ*(-)
						
						Sponge-miRNAs
						
							osa-miR810a 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	29488545-29488077(+) 29488116-29488086(-) 29487977-29488540(-)
Potential amino acid sequence	MLETLLVLENLKASDIHGDDVVHTQDFLVHFLQMEASLQVQVQDYGVSQNVHVSSQKHYQLYVE (+) MFAAGSFPVISPTPHKADNASATKHGRSGLHHSPAPAPVGLPPSEGNARGNPAYAPRHPHEYHS PSNSPGPAVSPASIIHPTNHGKAHGVPIAAHSKERHHHSMLVNNTHGNTHAGPVVAPPKGRHHH SLPVNNTRVKGPAYSPSNSPSIHRKHGIPVAAPPKQHSSNLPPSHHRPHKGMLTVKKKKVLISL RQCLLQDPFLS(-) MHEEILRMHHVIPMNITRLQILQDQQCLQHQ*(-)
Sponge-miRNAs	osa-miR810a
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os01g0709500_circ_g.1

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