Plant Circular RNA Database

Detail information of Os03g0429800_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os03g0429800_circ_g.4
					
						ID in PlantcircBase
						osa_circ_020384
					
						Alias
						Os_ciR8613
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 17987017-17987702  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os03g0429800						
					
						Parent gene annotation
						
							Similar to Xanthine dehydrogenase 1 (EC 1.1.1.204). (Os03t042980
0-01);Similar to Xanthine dehydrogenase 1 (EC 1.1.1.204). (Os03t
0429800-02);Similar to Aldehyde oxidase and xanthine dehydrogena
se, molybdopterin binding domain containing protein, expressed. 
(Os03t0429800-03);Similar to Aldehyde oxidase and xanthine dehyd
rogenase, molybdopterin binding domain containing protein, expre
ssed. (Os03t0429800-04)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0429800_circ_g.5 Os03g0429800_circ_g.6 Os03g0429800_circ_g.7 Os03g0429800_circ_g.8 						
					
						Support reads
						2/1
					
						Tissues
						root/shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0429800-02:2
Os03t0429800-01:2

General Information
CircRNA Name	Os03g0429800_circ_g.4
ID in PlantcircBase	osa_circ_020384
Alias	Os_ciR8613
Organism	Oryza sativa
Position	chr3: 17987017-17987702 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os03g0429800
Parent gene annotation	Similar to Xanthine dehydrogenase 1 (EC 1.1.1.204). (Os03t042980 0-01);Similar to Xanthine dehydrogenase 1 (EC 1.1.1.204). (Os03t 0429800-02);Similar to Aldehyde oxidase and xanthine dehydrogena se, molybdopterin binding domain containing protein, expressed. (Os03t0429800-03);Similar to Aldehyde oxidase and xanthine dehyd rogenase, molybdopterin binding domain containing protein, expre ssed. (Os03t0429800-04)
Parent gene strand	+
Alternative splicing	Os03g0429800_circ_g.5 Os03g0429800_circ_g.6 Os03g0429800_circ_g.7 Os03g0429800_circ_g.8
Support reads	2/1
Tissues	root/shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0429800-02:2 Os03t0429800-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AATCAACGCATGCTTGGCTCCGCTTTATTCTGTGGAAGGAATGCACATAATCACAGTAGAGGGA
ATTGGGAATCGTCAGCGAGGTTTGCACCCAATCCAGacattggtcttcctggaacaaagcttgg
atgtggtgaaggtggctgtggagcctgcactgtgatggtctcatgctatgatcaaactacaaag
aagacaca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 209
Transcript exons: 17987017-17987116,17987594-17987702
ACATTGGTCTTCCTGGAACAAAGCTTGGATGTGGTGAAGGTGGCTGTGGAGCCTGCACTGTGAT
GGTCTCATGCTATGATCAAACTACAAAGAAGACACAGCATTTTGCAATCAACGCATGCTTGGCT
CCGCTTTATTCTGTGGAAGGAATGCACATAATCACAGTAGAGGGAATTGGGAATCGTCAGCGAG
GTTTGCACCCAATCCAG
						
						Genomic sequence
						
							ACATTGGTCTTCCTGGAACAAAGCTTGGATGTGGTGAAGGTGGCTGTGGAGCCTGCACTGTGAT
GGTCTCATGCTATGATCAAACTACAAAGAAGACACAGTGAGAACTACATCCCTTCAACAAACCA
TGTTATGCAGTTTTTATCCAACTACAATGAAATCACTGTATCATGCAGTATTAGAATGAATATT
GCACCACCCTCACCCTTTATTCAGTTTTTCTTATACCTTGTTTTAAACAACCTTGAACTAGCAA
TAATTCGAGATACATTTGCAAGCTGCAGCTTGCTTGCATTTCTTGACCTTCCACATTGAACAGG
TGTTTTCTTATACCTTGTTTGAAACTATCTTGCAGTAGCTGTAAAGCGAGATACATTTGCATGC
TGCAGCTTTCTTACATTTCTTAACCTTCCATGTTTAACTAGTGTTTTGTGCTAGTATGCTATTC
TTAGATTTGAACGTAAGATCCTTAATATTGAACATCACATTTGTGGTTGTATACTTGTATCACG
TACCTGATATGTAATGGTCTGATTTTAGGATTTGAGCTTTACTTTGGTTAAAAAAACTTCATCA
GGCATTTTGCAATCAACGCATGCTTGGCTCCGCTTTATTCTGTGGAAGGAATGCACATAATCAC
AGTAGAGGGAATTGGGAATCGTCAGCGAGGTTTGCACCCAATCCAG

Sequences
Splice junction sequence	AATCAACGCATGCTTGGCTCCGCTTTATTCTGTGGAAGGAATGCACATAATCACAGTAGAGGGA ATTGGGAATCGTCAGCGAGGTTTGCACCCAATCCAGacattggtcttcctggaacaaagcttgg atgtggtgaaggtggctgtggagcctgcactgtgatggtctcatgctatgatcaaactacaaag aagacaca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 209 Transcript exons: 17987017-17987116,17987594-17987702 ACATTGGTCTTCCTGGAACAAAGCTTGGATGTGGTGAAGGTGGCTGTGGAGCCTGCACTGTGAT GGTCTCATGCTATGATCAAACTACAAAGAAGACACAGCATTTTGCAATCAACGCATGCTTGGCT CCGCTTTATTCTGTGGAAGGAATGCACATAATCACAGTAGAGGGAATTGGGAATCGTCAGCGAG GTTTGCACCCAATCCAG
Genomic sequence	ACATTGGTCTTCCTGGAACAAAGCTTGGATGTGGTGAAGGTGGCTGTGGAGCCTGCACTGTGAT GGTCTCATGCTATGATCAAACTACAAAGAAGACACAGTGAGAACTACATCCCTTCAACAAACCA TGTTATGCAGTTTTTATCCAACTACAATGAAATCACTGTATCATGCAGTATTAGAATGAATATT GCACCACCCTCACCCTTTATTCAGTTTTTCTTATACCTTGTTTTAAACAACCTTGAACTAGCAA TAATTCGAGATACATTTGCAAGCTGCAGCTTGCTTGCATTTCTTGACCTTCCACATTGAACAGG TGTTTTCTTATACCTTGTTTGAAACTATCTTGCAGTAGCTGTAAAGCGAGATACATTTGCATGC TGCAGCTTTCTTACATTTCTTAACCTTCCATGTTTAACTAGTGTTTTGTGCTAGTATGCTATTC TTAGATTTGAACGTAAGATCCTTAATATTGAACATCACATTTGTGGTTGTATACTTGTATCACG TACCTGATATGTAATGGTCTGATTTTAGGATTTGAGCTTTACTTTGGTTAAAAAAACTTCATCA GGCATTTTGCAATCAACGCATGCTTGGCTCCGCTTTATTCTGTGGAAGGAATGCACATAATCAC AGTAGAGGGAATTGGGAATCGTCAGCGAGGTTTGCACCCAATCCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.242673166

Conservation Information
Conserved circRNAs	NA
PMCS	0.242673166

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							17987613-17987076(+)
17987080-17987076(+)
17987090-17987087(-)
						
						Potential amino acid sequence
						
							MLGSALFCGRNAHNHSRGNWESSARFAPNPDIGLPGTKLGCGEGGCGACTVMVSCYDQTTKKTQ
HFAINACLAPLYSVEGMHIITVEGIGNRQRGLHPIQTLVFLEQSLDVVKVAVEPAL*(+)
MVSCYDQTTKKTQHFAINACLAPLYSVEGMHIITVEGIGNRQRGLHPIQTLVFLEQSLDVVKVA
VEPAL*(+)
MRPSQCRLHSHLHHIQALFQEDQCLDWVQTSLTIPNSLYCDYVHSFHRIKRSQACVDCKMLCLL
CSLIIA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	17987613-17987076(+) 17987080-17987076(+) 17987090-17987087(-)
Potential amino acid sequence	MLGSALFCGRNAHNHSRGNWESSARFAPNPDIGLPGTKLGCGEGGCGACTVMVSCYDQTTKKTQ HFAINACLAPLYSVEGMHIITVEGIGNRQRGLHPIQTLVFLEQSLDVVKVAVEPAL(+) MVSCYDQTTKKTQHFAINACLAPLYSVEGMHIITVEGIGNRQRGLHPIQTLVFLEQSLDVVKVA VEPAL(+) MRPSQCRLHSHLHHIQALFQEDQCLDWVQTSLTIPNSLYCDYVHSFHRIKRSQACVDCKMLCLL CSLIIA*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os03g0429800_circ_g.4

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