Plant Circular RNA Database

Detail information of Os06g0498400_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os06g0498400_circ_g.4
					
						ID in PlantcircBase
						osa_circ_030956
					
						Alias
						Os06circ10581
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 17491829-17493626  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os06g0498400						
					
						Parent gene annotation
						
							Similar to Alpha-glucan water dikinase, chloroplast precursor (E
C 2.7.9.4) (Starch-related R1 protein). (Os06t0498400-01);Simila
r to Alpha-glucan water dikinase (Fragment). (Os06t0498400-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os06g0498400_circ_g.1 Os06g0498400_circ_g.2 Os06g0498400_circ_g.3 Os06g0498400_circ_g.5 Os06g0498400_circ_g.6 						
					
						Support reads
						3
					
						Tissues
						leaf and panicle
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0498400-01:6

General Information
CircRNA Name	Os06g0498400_circ_g.4
ID in PlantcircBase	osa_circ_030956
Alias	Os06circ10581
Organism	Oryza sativa
Position	chr6: 17491829-17493626 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os06g0498400
Parent gene annotation	Similar to Alpha-glucan water dikinase, chloroplast precursor (E C 2.7.9.4) (Starch-related R1 protein). (Os06t0498400-01);Simila r to Alpha-glucan water dikinase (Fragment). (Os06t0498400-02)
Parent gene strand	+
Alternative splicing	Os06g0498400_circ_g.1 Os06g0498400_circ_g.2 Os06g0498400_circ_g.3 Os06g0498400_circ_g.5 Os06g0498400_circ_g.6
Support reads	3
Tissues	leaf and panicle
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0498400-01:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GGTATTGTAGAGAAATAATGACTGCAAAGGTGGAATGATGGAGGAGTGGCACCAGAAACTGCAC
AACAATACAAGCCCAGATGATGTAGTGATCTGCCAGgcacctccctcaagcatattgccatctg
gttcatcattgctagacaaggcatgtgaaacttcattcagtgaatatgaattgaatggtctgca
ttgtcagg

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GCACCTCCCTCAAGCATATTGCCATCTGGTTCATCATTGCTAGACAAGGCATGTGAAACTTCAT
TCAGTGAATATGAATTGAATGGTCTGCATTGTCAGGTATGGATAATTTTATCAGCCTATTATTT
TCCATTGTCTTTTACATTTCCTGTCATCTAAGTACTTCCCCATTTCCAGGTTGTTGAGATCGAG
CTTGACGATGGTGGATACAAGCGGATGCCCTTTGTTCTCCGGTCTGGTGAAACATGGATGAAAA
ATAATGGCTCTGACTTTTACTTGGATTTCAGCACCAAAGTTGCAAAAAATACAAAGGCAATTGC
TTCTGTCCACTTTTTGCATAGCAACCATGAAACTTTTTTCCCTTTGTTTTAAGGATAACTTTTT
TTACGACATGTTTTAAGGATAAATTCCTCATATGCCACTGAATATTTGCTATATCCCTTATTTT
CCACTCATTTCCTCACTGATATGTGTCAATTAGCGGCATGTAAGAGATGACTTAGGCCTTGTTC
GGTTATGCCCGGATTCAATCCGGACCGGGAATGACAAGTATAATAAGAAATTTATATAGATACA
ATGAGAGACCGGGATTTATTCCAGGCTGGGAACTAATTTACTCATGATATGGACAATAAGAGAC
CGGGAATCAATCCACTAGTCAAGAGGTGTAGAACTAATCTGAATGTTTTGTTGTCCCTATCATG
AGTAAATTGGTTCCCGATCTGGAATAAATCCCGGTCTCTTATTACATCTATCATAAATTTGTTA
CTATACTTCTCATTCCCGGCCCGGATTGAATCCAGATGTAACCAAACAAGGCCTTAAAAATTTT
CAATGTCAAATAAGAATATCTAAATAATTGTGGTAAAAATCACTTACTAATAAACTGTTGAATA
TATTCGTAATTAAAGGATACTGGTGATGCTGGTAAAGGCACTGCTAAGGCCTTGCTTGAAAGAA
TAGCAGATCTAGAGGAAGATGCCCAACGATCTCTTATGCACAGGTCAGGTGACACAATATCTAT
TACTTGTATGACTTGTTCTGTGTGTTAAAAAAATCCCTGAACTACCTTTATTCCTCTACAGATT
CAATATTGCAGCAGATCTAGTTGACCAAGCAAGAGATAATGGATTATTGGGTATTATTGGAATT
TTTGTTTGGATTAGGTTCATGGCTACAAGGCAACTAATATGGAACAAGAACTACAATGTGAAGC
CACGGTAGATACTTGTTTCATATTCTTCAGTGAATTTTCTCTCTGTGCAAAAAGAAATCAAATT
AACTACATAAGTACTTTTTTCTTGTGATGGGAATAAAAACCTTCTAGTCCTAATAAAGCATAAC
AGGAAAAAAAATAGAATGCTGTTTAGCAGACTGAACCTATTCTATTTGCATATCACACAAACAT
GACTTTGTTGCATCTTCTTTAACATCCATAATGATCTACAACTATGCAGTGAGATAAGCAAAGC
ACAAGATAGGTTTACAGATGATCTTGAGAATATGTACAGAACTTACCCACAATATCAGGAGATC
TTAAGAATGATAATGTCTGCTGTTGGTCGGGGAGGTGAAGGTGATGTTGGTCAACGCATTCGTG
ATGAGATATTAGTAATCCAGGTAAATTAATGTTCCTAGGTGAATATCAACTTGTTTGTTGATCA
ACTTGAACTATACAACAATAAATACTAGTTGGTTGGTATTGTAGAGAAATAATGACTGCAAAGG
TGGAATGATGGAGGAGTGGCACCAGAAACTGCACAACAATACAAGCCCAGATGATGTAGTGATC
TGCCAG
						
					

			
		

Sequences
Splice junction sequence	GGTATTGTAGAGAAATAATGACTGCAAAGGTGGAATGATGGAGGAGTGGCACCAGAAACTGCAC AACAATACAAGCCCAGATGATGTAGTGATCTGCCAGgcacctccctcaagcatattgccatctg gttcatcattgctagacaaggcatgtgaaacttcattcagtgaatatgaattgaatggtctgca ttgtcagg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GCACCTCCCTCAAGCATATTGCCATCTGGTTCATCATTGCTAGACAAGGCATGTGAAACTTCAT TCAGTGAATATGAATTGAATGGTCTGCATTGTCAGGTATGGATAATTTTATCAGCCTATTATTT TCCATTGTCTTTTACATTTCCTGTCATCTAAGTACTTCCCCATTTCCAGGTTGTTGAGATCGAG CTTGACGATGGTGGATACAAGCGGATGCCCTTTGTTCTCCGGTCTGGTGAAACATGGATGAAAA ATAATGGCTCTGACTTTTACTTGGATTTCAGCACCAAAGTTGCAAAAAATACAAAGGCAATTGC TTCTGTCCACTTTTTGCATAGCAACCATGAAACTTTTTTCCCTTTGTTTTAAGGATAACTTTTT TTACGACATGTTTTAAGGATAAATTCCTCATATGCCACTGAATATTTGCTATATCCCTTATTTT CCACTCATTTCCTCACTGATATGTGTCAATTAGCGGCATGTAAGAGATGACTTAGGCCTTGTTC GGTTATGCCCGGATTCAATCCGGACCGGGAATGACAAGTATAATAAGAAATTTATATAGATACA ATGAGAGACCGGGATTTATTCCAGGCTGGGAACTAATTTACTCATGATATGGACAATAAGAGAC CGGGAATCAATCCACTAGTCAAGAGGTGTAGAACTAATCTGAATGTTTTGTTGTCCCTATCATG AGTAAATTGGTTCCCGATCTGGAATAAATCCCGGTCTCTTATTACATCTATCATAAATTTGTTA CTATACTTCTCATTCCCGGCCCGGATTGAATCCAGATGTAACCAAACAAGGCCTTAAAAATTTT CAATGTCAAATAAGAATATCTAAATAATTGTGGTAAAAATCACTTACTAATAAACTGTTGAATA TATTCGTAATTAAAGGATACTGGTGATGCTGGTAAAGGCACTGCTAAGGCCTTGCTTGAAAGAA TAGCAGATCTAGAGGAAGATGCCCAACGATCTCTTATGCACAGGTCAGGTGACACAATATCTAT TACTTGTATGACTTGTTCTGTGTGTTAAAAAAATCCCTGAACTACCTTTATTCCTCTACAGATT CAATATTGCAGCAGATCTAGTTGACCAAGCAAGAGATAATGGATTATTGGGTATTATTGGAATT TTTGTTTGGATTAGGTTCATGGCTACAAGGCAACTAATATGGAACAAGAACTACAATGTGAAGC CACGGTAGATACTTGTTTCATATTCTTCAGTGAATTTTCTCTCTGTGCAAAAAGAAATCAAATT AACTACATAAGTACTTTTTTCTTGTGATGGGAATAAAAACCTTCTAGTCCTAATAAAGCATAAC AGGAAAAAAAATAGAATGCTGTTTAGCAGACTGAACCTATTCTATTTGCATATCACACAAACAT GACTTTGTTGCATCTTCTTTAACATCCATAATGATCTACAACTATGCAGTGAGATAAGCAAAGC ACAAGATAGGTTTACAGATGATCTTGAGAATATGTACAGAACTTACCCACAATATCAGGAGATC TTAAGAATGATAATGTCTGCTGTTGGTCGGGGAGGTGAAGGTGATGTTGGTCAACGCATTCGTG ATGAGATATTAGTAATCCAGGTAAATTAATGTTCCTAGGTGAATATCAACTTGTTTGTTGATCA ACTTGAACTATACAACAATAAATACTAGTTGGTTGGTATTGTAGAGAAATAATGACTGCAAAGG TGGAATGATGGAGGAGTGGCACCAGAAACTGCACAACAATACAAGCCCAGATGATGTAGTGATC TGCCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.14805654

Conservation Information
Conserved circRNAs	NA
PMCS	0.14805654

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							17492046-17493623(+)
17491847-17493417(-)
17491894-17493314(-)
						
						Potential amino acid sequence
						
							MPFVLRSGETWMKNNGSDFYLDFSTKVAKNTKDTGDAGKGTAKALLERIADLEEDAQRSLMHRF
NIAADLVDQARDNGLLGIIGIFVWIRFMATRQLIWNKNYNVKPREISKAQDRFTDDLENMYRTY
PQYQEILRMIMSAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQ
APPSSILPSGSSLLDKACETSFSEYELNGLHCQVVEIELDDGGYKRMPFVLRSGETWMKNNGSD
FYLDFSTKVAKNTKDTGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLVDQARDNGLLGI
IGIFVWIRFMATRQLIWNKNYNVKPREISKAQDRFTDDLENMYRTYPQYQEILRMIMSAVGRGG
EGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQAPPSSILPSGSSLLDKAC
ETSFSEYELNGLHCQVVEIELDDGGYKRMPFVLRSGETWMKNNGSDFYLDFSTKVAKNTKDTGD
AGKGTAKALLERIADLEEDAQRSLMHRFNIAADLVDQARDNGLLGIIGIFVWIRFMATRQLIWN
KNYNVKPREISKAQDRFTDDLENMYRTYPQYQEILRMIMSAVGRGGEGDVGQRIRDEILVIQRN
NDCKGGMMEEWHQKLHNNTSPDDVVICQAPPSSILPSGSSLLDKACETSFSEYELNGLHCQVVE
IELDDGGYKRMPFVLRSGETWMKNNGSDFYLDFSTKVAKNTKDTGDAGKGTAKALLERIADLEE
DAQRSLMHRFNIAADLVDQARDNGLLGIIGIFVWIRFMATRQLIWNKNYNVKPREISKAQDRFT
DDLENMYRTYPQYQEILRMIMSAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNN
TSPDDVVICQ(+)
MLEGGAWQITTSSGLVLLCSFWCHSSIIPPLQSLFLWITNISSRMR*(-)
MKFHMPCLAMMNQMAICLREVPGRSLHHLGLYCCAVSGATPPSFHLCSHYFSGLLISHHECVDQ
HHLHLPDQQQTLSFLRSPDIVGKFCTYSQDHL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	17492046-17493623(+) 17491847-17493417(-) 17491894-17493314(-)
Potential amino acid sequence	MPFVLRSGETWMKNNGSDFYLDFSTKVAKNTKDTGDAGKGTAKALLERIADLEEDAQRSLMHRF NIAADLVDQARDNGLLGIIGIFVWIRFMATRQLIWNKNYNVKPREISKAQDRFTDDLENMYRTY PQYQEILRMIMSAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQ APPSSILPSGSSLLDKACETSFSEYELNGLHCQVVEIELDDGGYKRMPFVLRSGETWMKNNGSD FYLDFSTKVAKNTKDTGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLVDQARDNGLLGI IGIFVWIRFMATRQLIWNKNYNVKPREISKAQDRFTDDLENMYRTYPQYQEILRMIMSAVGRGG EGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQAPPSSILPSGSSLLDKAC ETSFSEYELNGLHCQVVEIELDDGGYKRMPFVLRSGETWMKNNGSDFYLDFSTKVAKNTKDTGD AGKGTAKALLERIADLEEDAQRSLMHRFNIAADLVDQARDNGLLGIIGIFVWIRFMATRQLIWN KNYNVKPREISKAQDRFTDDLENMYRTYPQYQEILRMIMSAVGRGGEGDVGQRIRDEILVIQRN NDCKGGMMEEWHQKLHNNTSPDDVVICQAPPSSILPSGSSLLDKACETSFSEYELNGLHCQVVE IELDDGGYKRMPFVLRSGETWMKNNGSDFYLDFSTKVAKNTKDTGDAGKGTAKALLERIADLEE DAQRSLMHRFNIAADLVDQARDNGLLGIIGIFVWIRFMATRQLIWNKNYNVKPREISKAQDRFT DDLENMYRTYPQYQEILRMIMSAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNN TSPDDVVICQ(+) MLEGGAWQITTSSGLVLLCSFWCHSSIIPPLQSLFLWITNISSRMR(-) MKFHMPCLAMMNQMAICLREVPGRSLHHLGLYCCAVSGATPPSFHLCSHYFSGLLISHHECVDQ HHLHLPDQQQTLSFLRSPDIVGKFCTYSQDHL(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os06g0498400_circ_g.4

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