Plant Circular RNA Database

Detail information of orai.002G094900_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.002G094900_circ_g.2
					
						ID in PlantcircBase
						gra_circ_000141
					
						Alias
						Chr02:12014415|12016726
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr02: 12014415-12016726  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.002G094900						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							orai.002G094900_circ_g.3 						
					
						Support reads
						2
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.002G094900.2:5
Gorai.002G094900.1:5
Gorai.002G094900.3:5

General Information
CircRNA Name	orai.002G094900_circ_g.2
ID in PlantcircBase	gra_circ_000141
Alias	Chr02:12014415\|12016726
Organism	Gossypium raimondii
Position	chrChr02: 12014415-12016726 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.002G094900
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	orai.002G094900_circ_g.3
Support reads	2
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.002G094900.2:5 Gorai.002G094900.1:5 Gorai.002G094900.3:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCCAAAGTTCCAAAATGCTGGGAAGTACTTAAAGTTATCTGAATTTTTAAGCATGGCAAACACC
ACTAGCTCTCTAAATGGTGTCTTAATCAGCATAGAGtaagtcaaggtatctactctcggacgaa
taagcttgatggaaataattacccaattatgaccgttcaggatacctatactcaactgcaacca
cgtggatt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1091
Transcript exons: 12014415-12014530,12014661-12015168,12015657-12015695,12
015824-12016108,12016584-12016726
TAAGTCAAGGTATCTACTCTCGGACGAATAAGCTTGATGGAAATAATTACCCAATTATGACCGT
TCAGGATACCTATACTCAACTGCAACCACGTGGATTTTGGTTGAATATTCAGCATGATGCATTT
TATGCAGCACACAACTTGAGCATGAGAAGCTTTGTACTTTCTGTATCTAGAAATGCAAAAGTTA
CTGTTGACTATATCTCGTCACCGGAGGTAGCTTTCTTGCGAAACATTGCTCCAATTTTCCGAAG
AAGTTCAACAAAGGTTGTCTTCCGATTTCTAGGACCGAAGGATGTGGAACCTTCGACCAATCAG
ACCTATGATTCTCTCTTAACAAATCTCACATTTATCAAGACATTTGCCTCTGGAATCATTGTAC
CAAAGTCTTATATATGGCCTGTAGATGGAGCTCTTTACTTGCAACCTTCTACCTCTATTGTCTT
GGATGCTCACAAAGAAGGGCTAGAAGTTTTTGCGTCAGACTTTTATAATGATGTTCCATTCAGC
TTCAATTACTCATATGACCCAGTAGCTGAGTACCTTCAGTTTGTTGACAATGGTAAATTCTCTG
TTGATGGGGTTTTAACCGACTTCCCAATAACTCCAGCAGCAGCTATAGATTGTTTTGCTCACAT
AGGAAAAAATGCTTCAAAACAAGCGGACCTTTTGGTCATATCAAATAATGGAGCAAGTGGAGAC
TATCCTGGTTGTACTGACATGGCATATACAAAAGCTATTCAAGATGGTGTAGATGTTCTTGACT
GTCCTGTTCAAATGACAAAGGATGGAATACCTATTTGCTTGGGCTCTATAAATCTTATACAGAG
CACCGACATTGTCCGAACAGGCTTTAGCAATCTTACAGAAACTGTGCCAGAGATTATGCAGAGC
AGTGGAATATTTACCTACAACCTGTCATGGAGTGATATTAAAAGTTTGAAACCTGTAATATCAA
GCCCACAGTCACAGTACAAATTGTTTCGAAATCCAAAGTTCCAAAATGCTGGGAAGTACTTAAA
GTTATCTGAATTTTTAAGCATGGCAAACACCACTAGCTCTCTAAATGGTGTCTTAATCAGCATA
GAG
						
					

						Genomic sequence
						
							TAAGTCAAGGTATCTACTCTCGGACGAATAAGCTTGATGGAAATAATTACCCAATTATGACCGT
TCAGGATACCTATACTCAACTGCAACCACGTGGATTTTGGTTGAATATTCAGGTAAAGGATTTT
TCAGTGCTTAATAATGCGTTCCTACTTTTCCCTCATGTCTTTTCTCATCCCTTTGTTAATTTAG
TGTAACTTCATCTTTCTGTTTTTGGATTTAATTGACTGTCTCTCTATTTTGCAGCATGATGCAT
TTTATGCAGCACACAACTTGAGCATGAGAAGCTTTGTACTTTCTGTATCTAGAAATGCAAAAGT
TACTGTTGACTATATCTCGTCACCGGAGGTAGCTTTCTTGCGAAACATTGCTCCAATTTTCCGA
AGAAGTTCAACAAAGGTTGTCTTCCGATTTCTAGGACCGAAGGATGTGGAACCTTCGACCAATC
AGACCTATGATTCTCTCTTAACAAATCTCACATTTATCAAGACATTTGCCTCTGGAATCATTGT
ACCAAAGTCTTATATATGGCCTGTAGATGGAGCTCTTTACTTGCAACCTTCTACCTCTATTGTC
TTGGATGCTCACAAAGAAGGGCTAGAAGTTTTTGCGTCAGACTTTTATAATGATGTTCCATTCA
GCTTCAATTACTCATATGACCCAGTAGCTGAGTACCTTCAGTTTGTTGACAATGGTAAATTCTC
TGTTGATGGGGTTTTAACCGACTTCCCAATAACTCCAGCAGCAGCTATAGGTAAGTTATATGCA
GGTGTATGCATTATGAATCCTCAGAATTTATCAATTTATTTGTTGTTATTATTTTGCATCTATA
TGCTGATATTTACTTTCTAACTTAAATTATGGTCAAATTTCAAATTATTTTCGTTACTTGTCTT
AGTAACTTGCACTGCCATTTTGCAAATTTCATCAAAATTTGTGTACTTAGGGAGAATACTAAAA
GGGTGCGCCTAAGAATCTTTTGCTTTGTTGGTATTTTTCTCCTTGTTTGCCCCATGCCATGGTT
TCATCATTTAAATAAGATGGTGAAGTGAGATATCTGCCTTTTGTGTCAATCTGTATTTTCTTGG
CCTGACATTCTCTACAGTCTACACAGTAACTTAGTCTGGTTATTGAAAGGACAGGTTGCCTTAT
TTTTGCCAGATGCCATTATCTTTTGCTGCTACTGGATAAGAACGTTGGGGTTTTTTTGGTTGAG
ATGCTAACTTCTGTTTCGCATTGCAGATTGTTTTGCTCACATAGGAAAAAATGCTTCAAAACAA
GGTATGTATTTATGGAATGCTTCTGACCGGAATAGCTTAATATTTTTTGCTAAGAGTTAGTGGT
AATGGTCATGAGATTATGTGTTTTAAGGTTCTTCATAAACCATTTCTTTTGTTGTCATGCTACA
GCGGACCTTTTGGTCATATCAAATAATGGAGCAAGTGGAGACTATCCTGGTTGTACTGACATGG
CATATACAAAAGCTATTCAAGATGGTGTAGATGTTCTTGACTGTCCTGTTCAAATGACAAAGGA
TGGAATACCTATTTGCTTGGGCTCTATAAATCTTATACAGAGCACCGACATTGTCCGAACAGGC
TTTAGCAATCTTACAGAAACTGTGCCAGAGATTATGCAGAGCAGTGGAATATTTACCTACAACC
TGTCATGGAGTGATATTAAAAGTTTGAAACGTAAGTTGAATTTAGTGCTCGTTTGCTTGTTCTT
TATTGTATTGAATTGTTTTCTTTTTGATCTTTTTTTCTTTCTTTTGGGTGGGATTAGATTCTAG
AGTCTTTAATCTTTTCACAAAATAGAAACATAACATGTTGAAAATAAAAGTTGCCTAAGCATAG
ATTGAAGTTTGTACATTATATTGAGTGGGTTTATGAGATATGTCACACTTTCTGATGCAAATTC
ATGTTTCTTTCTGTGCTTGAAACATTGATAGCCATCTTGAAGGTGCATGAACGAAACTCTGCTT
TGGAAGATACTCTCTTTTTTCCTAGATTACTTATTGCAAGTTTATTTTAATTTGCCCCATCATC
TAAATCTGTGTTTTAGTTGTAGAGATTCAGCTAATTTTTTTTTTTGTTTCTTTGATAGATATAT
TTGGCATTAGCTTTTTGTCAAATTCCTTATTCTAATCTATCAATACTTCTCCTGTAGCTGTAAT
ATCAAGCCCACAGTCACAGTACAAATTGTTTCGAAATCCAAAGTTCCAAAATGCTGGGAAGTAC
TTAAAGTTATCTGAATTTTTAAGCATGGCAAACACCACTAGCTCTCTAAATGGTGTCTTAATCA
GCATAGAG
						
					

			
		

Sequences
Splice junction sequence	TCCAAAGTTCCAAAATGCTGGGAAGTACTTAAAGTTATCTGAATTTTTAAGCATGGCAAACACC ACTAGCTCTCTAAATGGTGTCTTAATCAGCATAGAGtaagtcaaggtatctactctcggacgaa taagcttgatggaaataattacccaattatgaccgttcaggatacctatactcaactgcaacca cgtggatt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1091 Transcript exons: 12014415-12014530,12014661-12015168,12015657-12015695,12 015824-12016108,12016584-12016726 TAAGTCAAGGTATCTACTCTCGGACGAATAAGCTTGATGGAAATAATTACCCAATTATGACCGT TCAGGATACCTATACTCAACTGCAACCACGTGGATTTTGGTTGAATATTCAGCATGATGCATTT TATGCAGCACACAACTTGAGCATGAGAAGCTTTGTACTTTCTGTATCTAGAAATGCAAAAGTTA CTGTTGACTATATCTCGTCACCGGAGGTAGCTTTCTTGCGAAACATTGCTCCAATTTTCCGAAG AAGTTCAACAAAGGTTGTCTTCCGATTTCTAGGACCGAAGGATGTGGAACCTTCGACCAATCAG ACCTATGATTCTCTCTTAACAAATCTCACATTTATCAAGACATTTGCCTCTGGAATCATTGTAC CAAAGTCTTATATATGGCCTGTAGATGGAGCTCTTTACTTGCAACCTTCTACCTCTATTGTCTT GGATGCTCACAAAGAAGGGCTAGAAGTTTTTGCGTCAGACTTTTATAATGATGTTCCATTCAGC TTCAATTACTCATATGACCCAGTAGCTGAGTACCTTCAGTTTGTTGACAATGGTAAATTCTCTG TTGATGGGGTTTTAACCGACTTCCCAATAACTCCAGCAGCAGCTATAGATTGTTTTGCTCACAT AGGAAAAAATGCTTCAAAACAAGCGGACCTTTTGGTCATATCAAATAATGGAGCAAGTGGAGAC TATCCTGGTTGTACTGACATGGCATATACAAAAGCTATTCAAGATGGTGTAGATGTTCTTGACT GTCCTGTTCAAATGACAAAGGATGGAATACCTATTTGCTTGGGCTCTATAAATCTTATACAGAG CACCGACATTGTCCGAACAGGCTTTAGCAATCTTACAGAAACTGTGCCAGAGATTATGCAGAGC AGTGGAATATTTACCTACAACCTGTCATGGAGTGATATTAAAAGTTTGAAACCTGTAATATCAA GCCCACAGTCACAGTACAAATTGTTTCGAAATCCAAAGTTCCAAAATGCTGGGAAGTACTTAAA GTTATCTGAATTTTTAAGCATGGCAAACACCACTAGCTCTCTAAATGGTGTCTTAATCAGCATA GAG
Genomic sequence	TAAGTCAAGGTATCTACTCTCGGACGAATAAGCTTGATGGAAATAATTACCCAATTATGACCGT TCAGGATACCTATACTCAACTGCAACCACGTGGATTTTGGTTGAATATTCAGGTAAAGGATTTT TCAGTGCTTAATAATGCGTTCCTACTTTTCCCTCATGTCTTTTCTCATCCCTTTGTTAATTTAG TGTAACTTCATCTTTCTGTTTTTGGATTTAATTGACTGTCTCTCTATTTTGCAGCATGATGCAT TTTATGCAGCACACAACTTGAGCATGAGAAGCTTTGTACTTTCTGTATCTAGAAATGCAAAAGT TACTGTTGACTATATCTCGTCACCGGAGGTAGCTTTCTTGCGAAACATTGCTCCAATTTTCCGA AGAAGTTCAACAAAGGTTGTCTTCCGATTTCTAGGACCGAAGGATGTGGAACCTTCGACCAATC AGACCTATGATTCTCTCTTAACAAATCTCACATTTATCAAGACATTTGCCTCTGGAATCATTGT ACCAAAGTCTTATATATGGCCTGTAGATGGAGCTCTTTACTTGCAACCTTCTACCTCTATTGTC TTGGATGCTCACAAAGAAGGGCTAGAAGTTTTTGCGTCAGACTTTTATAATGATGTTCCATTCA GCTTCAATTACTCATATGACCCAGTAGCTGAGTACCTTCAGTTTGTTGACAATGGTAAATTCTC TGTTGATGGGGTTTTAACCGACTTCCCAATAACTCCAGCAGCAGCTATAGGTAAGTTATATGCA GGTGTATGCATTATGAATCCTCAGAATTTATCAATTTATTTGTTGTTATTATTTTGCATCTATA TGCTGATATTTACTTTCTAACTTAAATTATGGTCAAATTTCAAATTATTTTCGTTACTTGTCTT AGTAACTTGCACTGCCATTTTGCAAATTTCATCAAAATTTGTGTACTTAGGGAGAATACTAAAA GGGTGCGCCTAAGAATCTTTTGCTTTGTTGGTATTTTTCTCCTTGTTTGCCCCATGCCATGGTT TCATCATTTAAATAAGATGGTGAAGTGAGATATCTGCCTTTTGTGTCAATCTGTATTTTCTTGG CCTGACATTCTCTACAGTCTACACAGTAACTTAGTCTGGTTATTGAAAGGACAGGTTGCCTTAT TTTTGCCAGATGCCATTATCTTTTGCTGCTACTGGATAAGAACGTTGGGGTTTTTTTGGTTGAG ATGCTAACTTCTGTTTCGCATTGCAGATTGTTTTGCTCACATAGGAAAAAATGCTTCAAAACAA GGTATGTATTTATGGAATGCTTCTGACCGGAATAGCTTAATATTTTTTGCTAAGAGTTAGTGGT AATGGTCATGAGATTATGTGTTTTAAGGTTCTTCATAAACCATTTCTTTTGTTGTCATGCTACA GCGGACCTTTTGGTCATATCAAATAATGGAGCAAGTGGAGACTATCCTGGTTGTACTGACATGG CATATACAAAAGCTATTCAAGATGGTGTAGATGTTCTTGACTGTCCTGTTCAAATGACAAAGGA TGGAATACCTATTTGCTTGGGCTCTATAAATCTTATACAGAGCACCGACATTGTCCGAACAGGC TTTAGCAATCTTACAGAAACTGTGCCAGAGATTATGCAGAGCAGTGGAATATTTACCTACAACC TGTCATGGAGTGATATTAAAAGTTTGAAACGTAAGTTGAATTTAGTGCTCGTTTGCTTGTTCTT TATTGTATTGAATTGTTTTCTTTTTGATCTTTTTTTCTTTCTTTTGGGTGGGATTAGATTCTAG AGTCTTTAATCTTTTCACAAAATAGAAACATAACATGTTGAAAATAAAAGTTGCCTAAGCATAG ATTGAAGTTTGTACATTATATTGAGTGGGTTTATGAGATATGTCACACTTTCTGATGCAAATTC ATGTTTCTTTCTGTGCTTGAAACATTGATAGCCATCTTGAAGGTGCATGAACGAAACTCTGCTT TGGAAGATACTCTCTTTTTTCCTAGATTACTTATTGCAAGTTTATTTTAATTTGCCCCATCATC TAAATCTGTGTTTTAGTTGTAGAGATTCAGCTAATTTTTTTTTTTGTTTCTTTGATAGATATAT TTGGCATTAGCTTTTTGTCAAATTCCTTATTCTAATCTATCAATACTTCTCCTGTAGCTGTAAT ATCAAGCCCACAGTCACAGTACAAATTGTTTCGAAATCCAAAGTTCCAAAATGCTGGGAAGTAC TTAAAGTTATCTGAATTTTTAAGCATGGCAAACACCACTAGCTCTCTAAATGGTGTCTTAATCA GCATAGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.450928831

Conservation Information
Conserved circRNAs	NA
PMCS	0.450928831

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12014664-12016073(-)
						
						Potential amino acid sequence
						
							MLNIQPKSTWLQLSIGILNGHNWVIISIKLIRPRVDTLTYSMLIKTPFRELVVFAMLKNSDNFK
YFPAFWNFGFRNNLYCDCGLDITGFKLLISLHDRL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12014664-12016073(-)
Potential amino acid sequence	MLNIQPKSTWLQLSIGILNGHNWVIISIKLIRPRVDTLTYSMLIKTPFRELVVFAMLKNSDNFK YFPAFWNFGFRNNLYCDCGLDITGFKLLISLHDRL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.002G094900_circ_g.2

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