Plant Circular RNA Database

Detail information of Os02g0475300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os02g0475300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_014670
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 16262046-16263009  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os02g0475300						
					
						Parent gene annotation
						
							Membrane attack complex component/perforin/complement C9 family 
protein. (Os02t0475300-01);Similar to MAC/Perforin domain contai
ning protein. (Os02t0475300-02);Similar to MAC/Perforin domain c
ontaining protein. (Os02t0475300-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0475300-02:4
Os02t0475300-03:4
Os02t0475300-01:4

General Information
CircRNA Name	Os02g0475300_circ_g.1
ID in PlantcircBase	osa_circ_014670
Alias	NA
Organism	Oryza sativa
Position	chr2: 16262046-16263009 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os02g0475300
Parent gene annotation	Membrane attack complex component/perforin/complement C9 family protein. (Os02t0475300-01);Similar to MAC/Perforin domain contai ning protein. (Os02t0475300-02);Similar to MAC/Perforin domain c ontaining protein. (Os02t0475300-03)
Parent gene strand	+
Alternative splicing	NA
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0475300-02:4 Os02t0475300-03:4 Os02t0475300-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GTTCAACGAGCTTATCCTTGGGCCTCAAAAGAGAAAAGGCTCCTATCCTTCATTGCAGTTCAGA
TTTCTTGGTCCCAAACTTCAAGTTAGCACTTCTCAGgtttatcaaaacttatgggacacacata
attgttgaaatggcagttgggggtcaggatgtcatttgtgttaagcaaagtccttcttcaacca
tttcatca

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTTTATCAAAACTTATGGGACACACATAATTGTTGAAATGGCAGTTGGGGGTCAGGATGTCATT
TGTGTTAAGCAAAGTCCTTCTTCAACCATTTCATCAGCGGACCTAAAGCTACACCTGGAAGATC
TTGGTGATTTCCTGTTTTCTGATGGGAGGAATCATTCCCCGATACATCGCAAGACCAGGGATGG
CAAGAGCAAGGTACAAAATGGTTCTACCCTCAATTCACGTAGCAACCTGCTGGAAGCAAACATA
TCTAATCCGATTCATTCAATATAGGTGCCTGATGTTTTTGTCAGGATGGAACAGCAGCCTAATA
ACCTACATCTTTCTAGCTACTCAGAATCATCAACCAAAGATGTGAGTATTTTTCTTAGTTTAAT
GTGTCAGTTGGTTCTCTAGCCGCGCTTTGCATACTGAAAGATCACTGCTTTCCCAAACAGGGCC
TGACAATTACATGCTCGAAAAGAGGTGGAGATGCCTCTATAGCCAGCCATTCCAAATGGTTACA
AACCGTTCCAAGGGTTCCTGATGCTATAATGTTTAAATTTGTTCCTATCACCTCTCTTCTTACC
GGCATTCCTGGTAGTGGTTACCTCAGCCATGCCATTAATTTGTATCTTCGCTGTAAGTTCTCCT
ATAAATTCCCTTTTCTGTTCCACAAACTTCAAATGTTAGTCTGTCATTCTATAATTGCTTCTTC
CGGATTGACTGCATTACTAACTTTCATAACCTTTATTCTGAATCGAACTGCAATTTGGATAAAT
GAATCCTTATAAAGTTTGTGCTCTGTCAGATAAACCTGATCCTGAAGACTTGCAACATTTCTTG
GAGTTTCAAGTGCCCCTACAATGGGCGCCTTTGTTCAACGAGCTTATCCTTGGGCCTCAAAAGA
GAAAAGGCTCCTATCCTTCATTGCAGTTCAGATTTCTTGGTCCCAAACTTCAAGTTAGCACTTC
TCAG

Sequences
Splice junction sequence	GTTCAACGAGCTTATCCTTGGGCCTCAAAAGAGAAAAGGCTCCTATCCTTCATTGCAGTTCAGA TTTCTTGGTCCCAAACTTCAAGTTAGCACTTCTCAGgtttatcaaaacttatgggacacacata attgttgaaatggcagttgggggtcaggatgtcatttgtgttaagcaaagtccttcttcaacca tttcatca
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTTTATCAAAACTTATGGGACACACATAATTGTTGAAATGGCAGTTGGGGGTCAGGATGTCATT TGTGTTAAGCAAAGTCCTTCTTCAACCATTTCATCAGCGGACCTAAAGCTACACCTGGAAGATC TTGGTGATTTCCTGTTTTCTGATGGGAGGAATCATTCCCCGATACATCGCAAGACCAGGGATGG CAAGAGCAAGGTACAAAATGGTTCTACCCTCAATTCACGTAGCAACCTGCTGGAAGCAAACATA TCTAATCCGATTCATTCAATATAGGTGCCTGATGTTTTTGTCAGGATGGAACAGCAGCCTAATA ACCTACATCTTTCTAGCTACTCAGAATCATCAACCAAAGATGTGAGTATTTTTCTTAGTTTAAT GTGTCAGTTGGTTCTCTAGCCGCGCTTTGCATACTGAAAGATCACTGCTTTCCCAAACAGGGCC TGACAATTACATGCTCGAAAAGAGGTGGAGATGCCTCTATAGCCAGCCATTCCAAATGGTTACA AACCGTTCCAAGGGTTCCTGATGCTATAATGTTTAAATTTGTTCCTATCACCTCTCTTCTTACC GGCATTCCTGGTAGTGGTTACCTCAGCCATGCCATTAATTTGTATCTTCGCTGTAAGTTCTCCT ATAAATTCCCTTTTCTGTTCCACAAACTTCAAATGTTAGTCTGTCATTCTATAATTGCTTCTTC CGGATTGACTGCATTACTAACTTTCATAACCTTTATTCTGAATCGAACTGCAATTTGGATAAAT GAATCCTTATAAAGTTTGTGCTCTGTCAGATAAACCTGATCCTGAAGACTTGCAACATTTCTTG GAGTTTCAAGTGCCCCTACAATGGGCGCCTTTGTTCAACGAGCTTATCCTTGGGCCTCAAAAGA GAAAAGGCTCCTATCCTTCATTGCAGTTCAGATTTCTTGGTCCCAAACTTCAAGTTAGCACTTC TCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.229275182

Conservation Information
Conserved circRNAs	NA
PMCS	0.229275182

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							16262084-16262078(+)
16262651-16262078(+)
						
						Potential amino acid sequence
						
							MAVGGQDVICVKQSPSSTISSADLKLHLEDLGDFLFSDGRNHSPIHRKTRDGKSKVPDVFVRME
QQPNNLHLSSYSESSTKDGLTITCSKRGGDASIASHSKWLQTVPRVPDAIMFKFVPITSLLTGI
PGSGYLSHAINLYLRYKPDPEDLQHFLEFQVPLQWAPLFNELILGPQKRKGSYPSLQFRFLGPK
LQVSTSQVYQNLWDTHNC*(+)
MPLICIFAINLILKTCNISWSFKCPYNGRLCSTSLSLGLKREKAPILHCSSDFLVPNFKLALLR
FIKTYGTHIIVEMAVGGQDVICVKQSPSSTISSADLKLHLEDLGDFLFSDGRNHSPIHRKTRDG
KSKVPDVFVRMEQQPNNLHLSSYSESSTKDGLTITCSKRGGDASIASHSKWLQTVPRVPDAIMF
KFVPITSLLTGIPGSGYLSHAINLYLRYKPDPEDLQHFLEFQVPLQWAPLFNELILGPQKRKGS
YPSLQFRFLGPKLQVSTSQVYQNLWDTHNC*(+)
						
						Sponge-miRNAs
						
							osa-miR1320-5p 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	16262084-16262078(+) 16262651-16262078(+)
Potential amino acid sequence	MAVGGQDVICVKQSPSSTISSADLKLHLEDLGDFLFSDGRNHSPIHRKTRDGKSKVPDVFVRME QQPNNLHLSSYSESSTKDGLTITCSKRGGDASIASHSKWLQTVPRVPDAIMFKFVPITSLLTGI PGSGYLSHAINLYLRYKPDPEDLQHFLEFQVPLQWAPLFNELILGPQKRKGSYPSLQFRFLGPK LQVSTSQVYQNLWDTHNC(+) MPLICIFAINLILKTCNISWSFKCPYNGRLCSTSLSLGLKREKAPILHCSSDFLVPNFKLALLR FIKTYGTHIIVEMAVGGQDVICVKQSPSSTISSADLKLHLEDLGDFLFSDGRNHSPIHRKTRDG KSKVPDVFVRMEQQPNNLHLSSYSESSTKDGLTITCSKRGGDASIASHSKWLQTVPRVPDAIMF KFVPITSLLTGIPGSGYLSHAINLYLRYKPDPEDLQHFLEFQVPLQWAPLFNELILGPQKRKGS YPSLQFRFLGPKLQVSTSQVYQNLWDTHNC(+)
Sponge-miRNAs	osa-miR1320-5p
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0475300_circ_g.1

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