Plant Circular RNA Database

Detail information of Csa1G000020_circ_g.4

			
						 General Information
					
						CircRNA Name
						Csa1G000020_circ_g.4
					
						ID in PlantcircBase
						csa_circ_000004
					
						Alias
						Chr1:8976_9670
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr1: 8976-9670  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2;Find_circ
					
						Parent gene
						
							Csa1G000020						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							Csa1G000020_circ_g.2 Csa1G000020_circ_g.3 Csa1G000020_circ_g.5 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa1G000020.1:2

General Information
CircRNA Name	Csa1G000020_circ_g.4
ID in PlantcircBase	csa_circ_000004
Alias	Chr1:8976_9670
Organism	Cucumis sativus
Position	chrChr1: 8976-9670 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2;Find_circ
Parent gene	Csa1G000020
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	Csa1G000020_circ_g.2 Csa1G000020_circ_g.3 Csa1G000020_circ_g.5
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa1G000020.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGAAGACAGATCCAAAATCCATCACTCTAGAATCTGGAGGAGCAGTTGCAGGTCCATCCACCAT
GCTGACTTTCCTAGAATACTCCATTTGGACTTTAACctccacatctctctgcattgtctttcga
cgcattagggaaattgcttcgtctgaatatggctcatcacgaccaggacatctcacacccgaga
aagcgaaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 585
Transcript exons: 8976-9209,9320-9670
CTCCACATCTCTCTGCATTGTCTTTCGACGCATTAGGGAAATTGCTTCGTCTGAATATGGCTCA
TCACGACCAGGACATCTCACACCCGAGAAAGCGAAAGCAATGCTACATGTCTCCTTTGGAATCA
ACAATTTGAAACGGTGACCACTGAGAACATATTCAACAACAGCTGGAATCCTCCTCCCACTCCG
GTGTAGGAATGGCAAAAAGTCTCGAGACTTTTTGGCTGGTGCTGTCAAAAGGTCTGTTACATGC
ATGACAGGAGGATCCTTGGCAGAATGAATTCCCTTCTTTCCAGCAATAGCACGTGATTCAGCAG
CAAGAAGTGCATCATAGTAGTTTGATCTCTCCTCAAAATCACGATGCCTAATCACTGTGCCAAA
ACCACGTGATACCACTAGCTCACCAACATTCACCCCAGCTTGTTGTTCACTGCTGTTTTTAGCA
GAATTATCCTCGCCCTCACCCTTAGTAGAAGACAATAGGAAGACAGATCCAAAATCCATCACTC
TAGAATCTGGAGGAGCAGTTGCAGGTCCATCCACCATGCTGACTTTCCTAGAATACTCCATTTG
GACTTTAAC
						
						Genomic sequence
						
							CTCCACATCTCTCTGCATTGTCTTTCGACGCATTAGGGAAATTGCTTCGTCTGAATATGGCTCA
TCACGACCAGGACATCTCACACCCGAGAAAGCGAAAGCAATGCTACATGTCTCCTTTGGAATCA
ACAATTTGAAACGGTGACCACTGAGAACATATTCAACAACAGCTGGAATCCTCCTCCCACTCCG
GTGTAGGAATGGCAAAAAGTCTCGAGACTTTTTGGCTGGTGCCTGGAAACAAACACATGATACT
TTGAATTCATTCAATAAGGTAAAATGTGTCATTAGTAACACTGGAAGGATAGAAGAAATTTAGT
ATAATGGATGGTAAAGTTTCTTACTGTCAAAAGGTCTGTTACATGCATGACAGGAGGATCCTTG
GCAGAATGAATTCCCTTCTTTCCAGCAATAGCACGTGATTCAGCAGCAAGAAGTGCATCATAGT
AGTTTGATCTCTCCTCAAAATCACGATGCCTAATCACTGTGCCAAAACCACGTGATACCACTAG
CTCACCAACATTCACCCCAGCTTGTTGTTCACTGCTGTTTTTAGCAGAATTATCCTCGCCCTCA
CCCTTAGTAGAAGACAATAGGAAGACAGATCCAAAATCCATCACTCTAGAATCTGGAGGAGCAG
TTGCAGGTCCATCCACCATGCTGACTTTCCTAGAATACTCCATTTGGACTTTAAC

Sequences
Splice junction sequence	GGAAGACAGATCCAAAATCCATCACTCTAGAATCTGGAGGAGCAGTTGCAGGTCCATCCACCAT GCTGACTTTCCTAGAATACTCCATTTGGACTTTAACctccacatctctctgcattgtctttcga cgcattagggaaattgcttcgtctgaatatggctcatcacgaccaggacatctcacacccgaga aagcgaaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 585 Transcript exons: 8976-9209,9320-9670 CTCCACATCTCTCTGCATTGTCTTTCGACGCATTAGGGAAATTGCTTCGTCTGAATATGGCTCA TCACGACCAGGACATCTCACACCCGAGAAAGCGAAAGCAATGCTACATGTCTCCTTTGGAATCA ACAATTTGAAACGGTGACCACTGAGAACATATTCAACAACAGCTGGAATCCTCCTCCCACTCCG GTGTAGGAATGGCAAAAAGTCTCGAGACTTTTTGGCTGGTGCTGTCAAAAGGTCTGTTACATGC ATGACAGGAGGATCCTTGGCAGAATGAATTCCCTTCTTTCCAGCAATAGCACGTGATTCAGCAG CAAGAAGTGCATCATAGTAGTTTGATCTCTCCTCAAAATCACGATGCCTAATCACTGTGCCAAA ACCACGTGATACCACTAGCTCACCAACATTCACCCCAGCTTGTTGTTCACTGCTGTTTTTAGCA GAATTATCCTCGCCCTCACCCTTAGTAGAAGACAATAGGAAGACAGATCCAAAATCCATCACTC TAGAATCTGGAGGAGCAGTTGCAGGTCCATCCACCATGCTGACTTTCCTAGAATACTCCATTTG GACTTTAAC
Genomic sequence	CTCCACATCTCTCTGCATTGTCTTTCGACGCATTAGGGAAATTGCTTCGTCTGAATATGGCTCA TCACGACCAGGACATCTCACACCCGAGAAAGCGAAAGCAATGCTACATGTCTCCTTTGGAATCA ACAATTTGAAACGGTGACCACTGAGAACATATTCAACAACAGCTGGAATCCTCCTCCCACTCCG GTGTAGGAATGGCAAAAAGTCTCGAGACTTTTTGGCTGGTGCCTGGAAACAAACACATGATACT TTGAATTCATTCAATAAGGTAAAATGTGTCATTAGTAACACTGGAAGGATAGAAGAAATTTAGT ATAATGGATGGTAAAGTTTCTTACTGTCAAAAGGTCTGTTACATGCATGACAGGAGGATCCTTG GCAGAATGAATTCCCTTCTTTCCAGCAATAGCACGTGATTCAGCAGCAAGAAGTGCATCATAGT AGTTTGATCTCTCCTCAAAATCACGATGCCTAATCACTGTGCCAAAACCACGTGATACCACTAG CTCACCAACATTCACCCCAGCTTGTTGTTCACTGCTGTTTTTAGCAGAATTATCCTCGCCCTCA CCCTTAGTAGAAGACAATAGGAAGACAGATCCAAAATCCATCACTCTAGAATCTGGAGGAGCAG TTGCAGGTCCATCCACCATGCTGACTTTCCTAGAATACTCCATTTGGACTTTAAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						1.159116799

Conservation Information
Conserved circRNAs	NA
PMCS	1.159116799

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9660-8978(-)
8985-9594(-)
						
						Potential amino acid sequence
						
							MEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNVGELVVSRGF
GTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKSRDFLPFLHR
SGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRRKTMQRDVEV
KVQMEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNVGELVVS
RGFGTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKSRDFLPF
LHRSGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRRKTMQRD
VEVKVQMEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNVGEL
VVSRGFGTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKSRDF
LPFLHRSGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRRKTM
QRDVEVKVQMEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNV
GELVVSRGFGTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKS
RDFLPFLHRSGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRR
KTMQRDVE
MWRLKSKWSILGKSAWWMDLQLLLQILE*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	9660-8978(-) 8985-9594(-)
Potential amino acid sequence	MEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNVGELVVSRGF GTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKSRDFLPFLHR SGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRRKTMQRDVEV KVQMEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNVGELVVS RGFGTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKSRDFLPF LHRSGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRRKTMQRD VEVKVQMEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNVGEL VVSRGFGTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKSRDF LPFLHRSGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRRKTM QRDVEVKVQMEYSRKVSMVDGPATAPPDSRVMDFGSVFLLSSTKGEGEDNSAKNSSEQQAGVNV GELVVSRGFGTVIRHRDFEERSNYYDALLAAESRAIAGKKGIHSAKDPPVMHVTDLLTAPAKKS RDFLPFLHRSGRRIPAVVEYVLSGHRFKLLIPKETCSIAFAFSGVRCPGRDEPYSDEAISLMRR KTMQRDVE MWRLKSKWSILGKSAWWMDLQLLLQILE*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019;He et al., 2020

circRNA

Detail information of Csa1G000020_circ_g.4

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