Plant Circular RNA Database

Detail information of 5_circ_ag.4

			
						 General Information
					
						CircRNA Name
						5_circ_ag.4
					
						ID in PlantcircBase
						ath_circ_038490
					
						Alias
						At_ciR313, Ath_circ_FC3325
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 5210223-5212022  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ag-circRNA							
									
						Identification method
						CIRI, find_circ
					
						Parent gene
						
							NA						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						NA
					
						Alternative splicing
						
							5_circ_ag.5 5_circ_ag.6 AT5G15970_circ_g.1 						
					
						Support reads
						27/235
					
						Tissues
						leaf/root, aerial, inflorescences, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA

General Information
CircRNA Name	5_circ_ag.4
ID in PlantcircBase	ath_circ_038490
Alias	At_ciR313, Ath_circ_FC3325
Organism	Arabidpsis thaliana
Position	chr5: 5210223-5212022 JBrowse»
Reference genome	TAIR10.38
Type	ag-circRNA
Identification method	CIRI, find_circ
Parent gene	NA
Parent gene annotation	NA
Parent gene strand	NA
Alternative splicing	5_circ_ag.5 5_circ_ag.6 AT5G15970_circ_g.1
Support reads	27/235
Tissues	leaf/root, aerial, inflorescences, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ACACACATCAAAAACGATTTTACAAGAAAAAAATATCTGAAAAATGTCAGAGACCAACAAGAAT
GCCTTCCAAGCCGGTCAGGCCGCTGGCAAAGCTGAGgagaagagcaatgttctgctggacaagg
ccaaggatgctgcagctggtgctggagctggagcacaacaggtaaacaatccatacacagacac
ataacata

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 178
Transcript exons: 5210223-5210290,5211913-5212022
GAGAAGAGCAATGTTCTGCTGGACAAGGCCAAGGATGCTGCAGCTGGTGCTGGAGCTGGAGCAC
AACACACAAACAAAACACACATCAAAAACGATTTTACAAGAAAAAAATATCTGAAAAATGTCAG
AGACCAACAAGAATGCCTTCCAAGCCGGTCAGGCCGCTGGCAAAGCTGAG
						
					

						Genomic sequence
						
							GAGAAGAGCAATGTTCTGCTGGACAAGGCCAAGGATGCTGCAGCTGGTGCTGGAGCTGGAGCAC
AACAGGTAAACAATCCATACACAGACACATAACATATAATATGTAACGAAATAAACGTCTTTGT
AAGCTTACATGTACGCAGATTTCTGATATGGTTATGTATATGTTATAGGCGGGAAAGAGTGTAT
CGGATGCGGCAGCGGGAGGTGTTAACTTCGTGAAGGACAAGACCGGCCTGAACAAGTAGCGATT
CGGGTCAAATTTGGGAGTTATAATTTCCCTTTTCTAATTAACTGTTGGGATTTTCAAATAAACG
ATCTTTGATCAAGAATTGCATTATATATATATAAATATATTGCAAAATTATTAGACGAGCCAAA
CTTATATTCAAATAATGTTTTATCTATTTTAAAAAATATTCTTTATGCGAAAGATCAAACTCCC
CAAACGATGTTAAATGGATCACGATACATAAGTCGATCCGAATTGTTGAAGTTTTCTAAAAATG
CAGTCCTTATAATTGTATTAAACACACTAAATCTTCCAAACACACAGAAGGAAATCACTATAAT
TGTTATAATGGATATAGGAGAACTAACTAAAAAAAAAAACGCAAAGAAAACTTTATACAGTACA
TACTCTTATGGTCTTACCAATTACCAACGATGTGTTGGTTACACAAGGTAAAATATATCGATCG
TAGTACTGGATTCATATTATTCATAATATGATGGGAAAATGAAGAATAAAAGTACATTTTGTAA
GTAGAATTCAACCATGAACTGTTTGGATTGGCAAACATTTTTTATTTGTTTTTGGCAAACATAA
ACTCAAATAAAATATCTAGGTATAATTGTGGTTCATACAAGAATTACTTCATACTGTTGGGCCA
AAGGGTACGTATCCTTCCCCGCACCTCCAAACCATGGGCTTACTACTGATCGGACATCAAAACC
TTGTTAGTTGCAACCAACGAATAATAAGTCAATACGATTCAACTTGTCAACAAATATACAACTT
ATATGACATGTCTGGCTCCAAACTGGATTTGAGTAGAAAGTTACTAATTCACAAAAACTAATTT
ATATACACATTTTCAATTTTTATTTTATAAATCAAAGAAAAAAAACATGAAAATACGGGAGGTT
CGGCAAACACAACATTTAACTTGCCAAACGTAGCATCTAACTATCCCACCTTCTACAAGGCACC
ATTTTTTTTTCAATAATAAAGTTTTTTTTCTTTAATTTCTTCGCCAAGAAGAGCACGAAATGTT
TGCCAAACGCATATGCAACAACCCCACGTTACATATTTCTATTTGTAGCTATAGAGCAAGCTAT
ATTGTTAAAAACTAAAAAGAAAATCTTTACTATAACATATAGATAGAGGATTCGAGATATCTTG
AAAGACTCAACTTAATAAATAAAGTCGAAAAGAAAACACGGAGGCGAGAGGACCACACACTCGC
ACAGAAAGAGTCTCATATCCTCTATAACAAATTGATAAACTAAACTAAAACGACACGTGATGTC
TTGATCAGCCAATAAAAAGCTACCGACATAAGGCAAAAATGATCGTACCATTAAACGTAATCCA
CGTGGTTTCAGATTACACGTGGCACCACACAAGTATCTCCATTTGGCCTATAAATATAAACCCT
TAAGCCCACATATCTTCTCAATCCATCACAAACAAAACACACATCAAAAACGATTTTACAAGAA
AAAAATATCTGAAAAATGTCAGAGACCAACAAGAATGCCTTCCAAGCCGGTCAGGCCGCTGGCA
AAGCTGAG
						
					

			
		

Sequences
Splice junction sequence	ACACACATCAAAAACGATTTTACAAGAAAAAAATATCTGAAAAATGTCAGAGACCAACAAGAAT GCCTTCCAAGCCGGTCAGGCCGCTGGCAAAGCTGAGgagaagagcaatgttctgctggacaagg ccaaggatgctgcagctggtgctggagctggagcacaacaggtaaacaatccatacacagacac ataacata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 178 Transcript exons: 5210223-5210290,5211913-5212022 GAGAAGAGCAATGTTCTGCTGGACAAGGCCAAGGATGCTGCAGCTGGTGCTGGAGCTGGAGCAC AACACACAAACAAAACACACATCAAAAACGATTTTACAAGAAAAAAATATCTGAAAAATGTCAG AGACCAACAAGAATGCCTTCCAAGCCGGTCAGGCCGCTGGCAAAGCTGAG
Genomic sequence	GAGAAGAGCAATGTTCTGCTGGACAAGGCCAAGGATGCTGCAGCTGGTGCTGGAGCTGGAGCAC AACAGGTAAACAATCCATACACAGACACATAACATATAATATGTAACGAAATAAACGTCTTTGT AAGCTTACATGTACGCAGATTTCTGATATGGTTATGTATATGTTATAGGCGGGAAAGAGTGTAT CGGATGCGGCAGCGGGAGGTGTTAACTTCGTGAAGGACAAGACCGGCCTGAACAAGTAGCGATT CGGGTCAAATTTGGGAGTTATAATTTCCCTTTTCTAATTAACTGTTGGGATTTTCAAATAAACG ATCTTTGATCAAGAATTGCATTATATATATATAAATATATTGCAAAATTATTAGACGAGCCAAA CTTATATTCAAATAATGTTTTATCTATTTTAAAAAATATTCTTTATGCGAAAGATCAAACTCCC CAAACGATGTTAAATGGATCACGATACATAAGTCGATCCGAATTGTTGAAGTTTTCTAAAAATG CAGTCCTTATAATTGTATTAAACACACTAAATCTTCCAAACACACAGAAGGAAATCACTATAAT TGTTATAATGGATATAGGAGAACTAACTAAAAAAAAAAACGCAAAGAAAACTTTATACAGTACA TACTCTTATGGTCTTACCAATTACCAACGATGTGTTGGTTACACAAGGTAAAATATATCGATCG TAGTACTGGATTCATATTATTCATAATATGATGGGAAAATGAAGAATAAAAGTACATTTTGTAA GTAGAATTCAACCATGAACTGTTTGGATTGGCAAACATTTTTTATTTGTTTTTGGCAAACATAA ACTCAAATAAAATATCTAGGTATAATTGTGGTTCATACAAGAATTACTTCATACTGTTGGGCCA AAGGGTACGTATCCTTCCCCGCACCTCCAAACCATGGGCTTACTACTGATCGGACATCAAAACC TTGTTAGTTGCAACCAACGAATAATAAGTCAATACGATTCAACTTGTCAACAAATATACAACTT ATATGACATGTCTGGCTCCAAACTGGATTTGAGTAGAAAGTTACTAATTCACAAAAACTAATTT ATATACACATTTTCAATTTTTATTTTATAAATCAAAGAAAAAAAACATGAAAATACGGGAGGTT CGGCAAACACAACATTTAACTTGCCAAACGTAGCATCTAACTATCCCACCTTCTACAAGGCACC ATTTTTTTTTCAATAATAAAGTTTTTTTTCTTTAATTTCTTCGCCAAGAAGAGCACGAAATGTT TGCCAAACGCATATGCAACAACCCCACGTTACATATTTCTATTTGTAGCTATAGAGCAAGCTAT ATTGTTAAAAACTAAAAAGAAAATCTTTACTATAACATATAGATAGAGGATTCGAGATATCTTG AAAGACTCAACTTAATAAATAAAGTCGAAAAGAAAACACGGAGGCGAGAGGACCACACACTCGC ACAGAAAGAGTCTCATATCCTCTATAACAAATTGATAAACTAAACTAAAACGACACGTGATGTC TTGATCAGCCAATAAAAAGCTACCGACATAAGGCAAAAATGATCGTACCATTAAACGTAATCCA CGTGGTTTCAGATTACACGTGGCACCACACAAGTATCTCCATTTGGCCTATAAATATAAACCCT TAAGCCCACATATCTTCTCAATCCATCACAAACAAAACACACATCAAAAACGATTTTACAAGAA AAAAATATCTGAAAAATGTCAGAGACCAACAAGAATGCCTTCCAAGCCGGTCAGGCCGCTGGCA AAGCTGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.785891087

Conservation Information
Conserved circRNAs	NA
PMCS	0.785891087

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of 5_circ_ag.4

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