Plant Circular RNA Database

Detail information of ENSRNA049471535_circ_g.4

			
						 General Information
					
						CircRNA Name
						ENSRNA049471535_circ_g.4
					
						ID in PlantcircBase
						osa_circ_002690
					
						Alias
						Os_ciR3513, 
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 24615789-24615989  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ, CIRI-long
					
						Parent gene
						
							EPlOSAG00000045065						
					
						Parent gene annotation
						
							ncRNA
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0618700_circ_ig.1 ENSRNA049471535_circ_igg.1 ENSRNA049471535_circ_igg.2 ENSRNA049471535_circ_igg.3 ENSRNA049471535_circ_igg.4 ENSRNA049471535_circ_igg.5 ENSRNA049471535_circ_igg.6 Os01g0618700_circ_ig.7 Os01g0618700_circ_ig.8 Os01g0618700_circ_ig.9 ENSRNA049471535_circ_g.1 ENSRNA049471535_circ_g.2 ENSRNA049471535_circ_g.3 ENSRNA049471535_circ_igg.5 ENSRNA049471535_circ_g.6 ENSRNA049471535_circ_igg.7 						
					
						Support reads
						2
					
						Tissues
						root, root, leaf
					
						Exon boundary  
						
							No-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						ENSRNA049471535-T1:1
ENSRNA049471535-T1:1
EPlOSAT00000046453:1

General Information
CircRNA Name	ENSRNA049471535_circ_g.4
ID in PlantcircBase	osa_circ_002690
Alias	Os_ciR3513,
Organism	Oryza sativa
Position	chr1: 24615789-24615989 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ, CIRI-long
Parent gene	EPlOSAG00000045065
Parent gene annotation	ncRNA
Parent gene strand	+
Alternative splicing	Os01g0618700_circ_ig.1 ENSRNA049471535_circ_igg.1 ENSRNA049471535_circ_igg.2 ENSRNA049471535_circ_igg.3 ENSRNA049471535_circ_igg.4 ENSRNA049471535_circ_igg.5 ENSRNA049471535_circ_igg.6 Os01g0618700_circ_ig.7 Os01g0618700_circ_ig.8 Os01g0618700_circ_ig.9 ENSRNA049471535_circ_g.1 ENSRNA049471535_circ_g.2 ENSRNA049471535_circ_g.3 ENSRNA049471535_circ_igg.5 ENSRNA049471535_circ_g.6 ENSRNA049471535_circ_igg.7
Support reads	2
Tissues	root, root, leaf
Exon boundary	No-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	ENSRNA049471535-T1:1 ENSRNA049471535-T1:1 EPlOSAT00000046453:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCGCCCTCCTCTTCGCGAGGGGGCAGAGACACGTGTGGAGCCCTTTGGGCGCGAGCCGAGATCA
TAATTGGCCAATTACACAGTTATTCACTTGATCCATgagcacatgacgaagtgagcgggctctt
tgtagccctggaaagtccccgggccctcaaccgcagagatgcggccgcggagacgcggaaagtc
tcagcgct

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 201
Transcript exons: 24615789-24615989
GAGCACATGACGAAGTGAGCGGGCTCTTTGTAGCCCTGGAAAGTCCCCGGGCCCTCAACCGCAG
AGATGCGGCCGCGGAGACGCGGAAAGTCTCAGCGCTCGCGCCCTCCTCTTCGCGAGGGGGCAGA
GACACGTGTGGAGCCCTTTGGGCGCGAGCCGAGATCATAATTGGCCAATTACACAGTTATTCAC
TTGATCCAT
						
						Genomic sequence
						
							GAGCACATGACGAAGTGAGCGGGCTCTTTGTAGCCCTGGAAAGTCCCCGGGCCCTCAACCGCAG
AGATGCGGCCGCGGAGACGCGGAAAGTCTCAGCGCTCGCGCCCTCCTCTTCGCGAGGGGGCAGA
GACACGTGTGGAGCCCTTTGGGCGCGAGCCGAGATCATAATTGGCCAATTACACAGTTATTCAC
TTGATCCAT

Sequences
Splice junction sequence	GCGCCCTCCTCTTCGCGAGGGGGCAGAGACACGTGTGGAGCCCTTTGGGCGCGAGCCGAGATCA TAATTGGCCAATTACACAGTTATTCACTTGATCCATgagcacatgacgaagtgagcgggctctt tgtagccctggaaagtccccgggccctcaaccgcagagatgcggccgcggagacgcggaaagtc tcagcgct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 201 Transcript exons: 24615789-24615989 GAGCACATGACGAAGTGAGCGGGCTCTTTGTAGCCCTGGAAAGTCCCCGGGCCCTCAACCGCAG AGATGCGGCCGCGGAGACGCGGAAAGTCTCAGCGCTCGCGCCCTCCTCTTCGCGAGGGGGCAGA GACACGTGTGGAGCCCTTTGGGCGCGAGCCGAGATCATAATTGGCCAATTACACAGTTATTCAC TTGATCCAT
Genomic sequence	GAGCACATGACGAAGTGAGCGGGCTCTTTGTAGCCCTGGAAAGTCCCCGGGCCCTCAACCGCAG AGATGCGGCCGCGGAGACGCGGAAAGTCTCAGCGCTCGCGCCCTCCTCTTCGCGAGGGGGCAGA GACACGTGTGGAGCCCTTTGGGCGCGAGCCGAGATCATAATTGGCCAATTACACAGTTATTCAC TTGATCCAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.28490796

Conservation Information
Conserved circRNAs	NA
PMCS	0.28490796

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015; this study

circRNA

Detail information of ENSRNA049471535_circ_g.4

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