Plant Circular RNA Database

Detail information of Zm00001d028915_circ_g.5

			
						 General Information
					
						CircRNA Name
						Zm00001d028915_circ_g.5
					
						ID in PlantcircBase
						zma_circ_006501
					
						Alias
						Zm01circ00058, zma_circ_0000126
					
						Organism
						
							Zea mays						
					
						Position
						
							chr1: 51116352-51126544  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d028915						
					
						Parent gene annotation
						
							Chloroplast protein HCF243
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d028915_circ_g.2 Zm00001d028915_circ_g.3 Zm00001d028915_circ_g.4 Zm00001d028915_circ_g.6 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d028915_T010:3
Zm00001d028915_T012:2
Zm00001d028915_T003:3
Zm00001d028915_T011:4
Zm00001d028915_T002:3
Zm00001d028915_T004:3
Zm00001d028915_T005:3
Zm00001d028915_T009:3
Zm00001d028915_T001:3

General Information
CircRNA Name	Zm00001d028915_circ_g.5
ID in PlantcircBase	zma_circ_006501
Alias	Zm01circ00058, zma_circ_0000126
Organism	Zea mays
Position	chr1: 51116352-51126544 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d028915
Parent gene annotation	Chloroplast protein HCF243
Parent gene strand	+
Alternative splicing	Zm00001d028915_circ_g.2 Zm00001d028915_circ_g.3 Zm00001d028915_circ_g.4 Zm00001d028915_circ_g.6
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d028915_T010:3 Zm00001d028915_T012:2 Zm00001d028915_T003:3 Zm00001d028915_T011:4 Zm00001d028915_T002:3 Zm00001d028915_T004:3 Zm00001d028915_T005:3 Zm00001d028915_T009:3 Zm00001d028915_T001:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTGTGGAGAGAACTGGGGGTGAACTCTTGGCCTACATTTGTTCTTATTGGACCCAACGGAAAG
GTTCTAGCACAGATATCAGGTGAAGGTCATAGAAAGgatttgaaaggaagagtggtgctgctgg
atttttggacatattgctgcattaactgcatgcatgttttgccagacctggattttgtagagaa
gaagtata

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 330
Transcript exons: 51116352-51116462,51123926-51124021,51126422-51126544
GATTTGAAAGGAAGAGTGGTGCTGCTGGATTTTTGGACATATTGCTGCATTAACTGCATGCATG
TTTTGCCAGACCTGGATTTTGTAGAGAAGAAGTATAAAGAAAAACCTTTCACTGTTGTTGGTGT
GCACTCTGCCAAATTCGATAATGAAAAGGACCTTGAAGCTATACGTAATGCTGTTCTGCGCTAC
AACATTACTCACCCGGTTGTAAATGATGGTGACATGTACTTGTGGAGAGAACTGGGGGTGAACT
CTTGGCCTACATTTGTTCTTATTGGACCCAACGGAAAGGTTCTAGCACAGATATCAGGTGAAGG
TCATAGAAAG
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CTTGTGGAGAGAACTGGGGGTGAACTCTTGGCCTACATTTGTTCTTATTGGACCCAACGGAAAG GTTCTAGCACAGATATCAGGTGAAGGTCATAGAAAGgatttgaaaggaagagtggtgctgctgg atttttggacatattgctgcattaactgcatgcatgttttgccagacctggattttgtagagaa gaagtata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 330 Transcript exons: 51116352-51116462,51123926-51124021,51126422-51126544 GATTTGAAAGGAAGAGTGGTGCTGCTGGATTTTTGGACATATTGCTGCATTAACTGCATGCATG TTTTGCCAGACCTGGATTTTGTAGAGAAGAAGTATAAAGAAAAACCTTTCACTGTTGTTGGTGT GCACTCTGCCAAATTCGATAATGAAAAGGACCTTGAAGCTATACGTAATGCTGTTCTGCGCTAC AACATTACTCACCCGGTTGTAAATGATGGTGACATGTACTTGTGGAGAGAACTGGGGGTGAACT CTTGGCCTACATTTGTTCTTATTGGACCCAACGGAAAGGTTCTAGCACAGATATCAGGTGAAGG TCATAGAAAG
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.088514313

Conservation Information
Conserved circRNAs	NA
PMCS	0.088514313

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							51116410-51126541(+)
51116416-51126526(-)
51116412-51126537(-)
51116393-51126491(-)
						
						Potential amino acid sequence
						
							MHVLPDLDFVEKKYKEKPFTVVGVHSAKFDNEKDLEAIRNAVLRYNITHPVVNDGDMYLWRELG
VNSWPTFVLIGPNGKVLAQISGEGHRKDLKGRVVLLDFWTYCCINCMHVLPDLDFVEKKYKEKP
FTVVGVHSAKFDNEKDLEAIRNAVLRYNITHPVVNDGDMYLWRELGVNSWPTFVLIGPNGKVLA
QISGEGHRKDLKGRVVLLDFWTYCCINCMHVLPDLDFVEKKYKEKPFTVVGVHSAKFDNEKDLE
AIRNAVLRYNITHPVVNDGDMYLWRELGVNSWPTFVLIGPNGKVLAQISGEGHRKDLKGRVVLL
DFWTYCCINCMHVLPDLDFVEKKYKEKPFTVVGVHSAKFDNEKDLEAIRNAVLRYNITHPVVND
GDMYLWRELGVNSWPTFVLIGPNGKVLAQISGEGHRK(+)
MHAVNAAICPKIQQHHSSFQILSMTFT*(-)
MQLMQQYVQKSSSTTLPFKSFL*(-)
MSKNPAAPLFLSNPFYDLHLISVLEPFRWVQ*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	51116410-51126541(+) 51116416-51126526(-) 51116412-51126537(-) 51116393-51126491(-)
Potential amino acid sequence	MHVLPDLDFVEKKYKEKPFTVVGVHSAKFDNEKDLEAIRNAVLRYNITHPVVNDGDMYLWRELG VNSWPTFVLIGPNGKVLAQISGEGHRKDLKGRVVLLDFWTYCCINCMHVLPDLDFVEKKYKEKP FTVVGVHSAKFDNEKDLEAIRNAVLRYNITHPVVNDGDMYLWRELGVNSWPTFVLIGPNGKVLA QISGEGHRKDLKGRVVLLDFWTYCCINCMHVLPDLDFVEKKYKEKPFTVVGVHSAKFDNEKDLE AIRNAVLRYNITHPVVNDGDMYLWRELGVNSWPTFVLIGPNGKVLAQISGEGHRKDLKGRVVLL DFWTYCCINCMHVLPDLDFVEKKYKEKPFTVVGVHSAKFDNEKDLEAIRNAVLRYNITHPVVND GDMYLWRELGVNSWPTFVLIGPNGKVLAQISGEGHRK(+) MHAVNAAICPKIQQHHSSFQILSMTFT(-) MQLMQQYVQKSSSTTLPFKSFL(-) MSKNPAAPLFLSNPFYDLHLISVLEPFRWVQ*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b

circRNA

Detail information of Zm00001d028915_circ_g.5

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