Plant Circular RNA Database

Detail information of Os07g0161000_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os07g0161000_circ_g.1
					
						ID in PlantcircBase
						osa_circ_032841
					
						Alias
						Os_ciR11218
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr7: 3271250-3272141  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os07g0161000						
					
						Parent gene annotation
						
							Tetratricopeptide-like helical domain containing protein. (Os07t
0161000-01);Hypothetical conserved gene. (Os07t0161000-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						2/1
					
						Tissues
						root/shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os07t0161000-01:2
Os07t0161000-02:2

General Information
CircRNA Name	Os07g0161000_circ_g.1
ID in PlantcircBase	osa_circ_032841
Alias	Os_ciR11218
Organism	Oryza sativa
Position	chr7: 3271250-3272141 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os07g0161000
Parent gene annotation	Tetratricopeptide-like helical domain containing protein. (Os07t 0161000-01);Hypothetical conserved gene. (Os07t0161000-02)
Parent gene strand	-
Alternative splicing	NA
Support reads	2/1
Tissues	root/shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os07t0161000-01:2 Os07t0161000-02:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CCTTCTTCAGGAGGTTGTGAAACAGTGTATCCAGGGGATGAACTTCTTTGACTGCTTGATGGCT
CAGAAGCATCATCTACAACTTCTTCAATGACAACACcattctatccaaagcctcaggagataat
gaagacaaggcttcttgagctttggcagcatcctccttcgtcattttcattccaaactgctcac
tcatgctt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 794
Transcript exons: 3271250-3271393,3271492-3272141
CATTCTATCCAAAGCCTCAGGAGATAATGAAGACAAGGCTTCTTGAGCTTTGGCAGCATCCTCC
TTCGTCATTTTCATTCCAAACTGCTCACTCATGCTTGACATCATCTCTGGACTGATGTTCTTCA
TCATGGATGCATACATCTTCCGCATCGCAGGATCTTTCATAGCATTTCTCATGGTTTCCTGCAT
ATCAGCTGTAGAAGGTGCACCAGGGGATGATGATTCACCCATCCTTTGGTTGCTTAATATTTCA
TGAGGATTTTCCATGACATTTTGAGAAGATGAAGGTTGGCTGTTATCCACTGATCTCTGGGAAG
TACTACTTGTTGTGGCTCTTGATGAAGATTGAAGCTTATTCTCTGGTCTCAAGGGGGAAGCACT
AGGTCCACCCATATTAGAAGCAAAATTCAGCATATTTTGTAATTCATCAGGAGACATATTCCCA
ATCATGTCAGATGCCATCTTAAACATCTCAGGAGACATTTCTGGCATATTAGGTCCCAAATTTG
GAGGACTGGAGCTTGTTCCGTGCAACGAAGAAGCAGCTTCAAACATTTTCTGAAGTTCTTCTGG
TTTCATTGTGCCAATCATATCGCTGGCAGTTTTTACTAATTCAGGTGACATTCCCTGCATTCCC
AGCTTCGATAAGCCATTAGCATCTATGCTTGAAGACCCTGAAGGTCTAGAGTTCCCTTCTTCAG
GAGGTTGTGAAACAGTGTATCCAGGGGATGAACTTCTTTGACTGCTTGATGGCTCAGAAGCATC
ATCTACAACTTCTTCAATGACAACAC
						
					

						Genomic sequence
						
							CATTCTATCCAAAGCCTCAGGAGATAATGAAGACAAGGCTTCTTGAGCTTTGGCAGCATCCTCC
TTCGTCATTTTCATTCCAAACTGCTCACTCATGCTTGACATCATCTCTGGACTGATGTTCTTCA
TCATGGATGCATACATCTGTTGATTTAGAAAAAGACTGTTAAAATCCAAAGCCTACCATTTTTT
GCTAGGTAACAAGTTCATTCAAAATTTCCTTCTGTTCGAAGATTCTATACCTTCCGCATCGCAG
GATCTTTCATAGCATTTCTCATGGTTTCCTGCATATCAGCTGTAGAAGGTGCACCAGGGGATGA
TGATTCACCCATCCTTTGGTTGCTTAATATTTCATGAGGATTTTCCATGACATTTTGAGAAGAT
GAAGGTTGGCTGTTATCCACTGATCTCTGGGAAGTACTACTTGTTGTGGCTCTTGATGAAGATT
GAAGCTTATTCTCTGGTCTCAAGGGGGAAGCACTAGGTCCACCCATATTAGAAGCAAAATTCAG
CATATTTTGTAATTCATCAGGAGACATATTCCCAATCATGTCAGATGCCATCTTAAACATCTCA
GGAGACATTTCTGGCATATTAGGTCCCAAATTTGGAGGACTGGAGCTTGTTCCGTGCAACGAAG
AAGCAGCTTCAAACATTTTCTGAAGTTCTTCTGGTTTCATTGTGCCAATCATATCGCTGGCAGT
TTTTACTAATTCAGGTGACATTCCCTGCATTCCCAGCTTCGATAAGCCATTAGCATCTATGCTT
GAAGACCCTGAAGGTCTAGAGTTCCCTTCTTCAGGAGGTTGTGAAACAGTGTATCCAGGGGATG
AACTTCTTTGACTGCTTGATGGCTCAGAAGCATCATCTACAACTTCTTCAATGACAACAC
						
					

			
		

Sequences
Splice junction sequence	CCTTCTTCAGGAGGTTGTGAAACAGTGTATCCAGGGGATGAACTTCTTTGACTGCTTGATGGCT CAGAAGCATCATCTACAACTTCTTCAATGACAACACcattctatccaaagcctcaggagataat gaagacaaggcttcttgagctttggcagcatcctccttcgtcattttcattccaaactgctcac tcatgctt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 794 Transcript exons: 3271250-3271393,3271492-3272141 CATTCTATCCAAAGCCTCAGGAGATAATGAAGACAAGGCTTCTTGAGCTTTGGCAGCATCCTCC TTCGTCATTTTCATTCCAAACTGCTCACTCATGCTTGACATCATCTCTGGACTGATGTTCTTCA TCATGGATGCATACATCTTCCGCATCGCAGGATCTTTCATAGCATTTCTCATGGTTTCCTGCAT ATCAGCTGTAGAAGGTGCACCAGGGGATGATGATTCACCCATCCTTTGGTTGCTTAATATTTCA TGAGGATTTTCCATGACATTTTGAGAAGATGAAGGTTGGCTGTTATCCACTGATCTCTGGGAAG TACTACTTGTTGTGGCTCTTGATGAAGATTGAAGCTTATTCTCTGGTCTCAAGGGGGAAGCACT AGGTCCACCCATATTAGAAGCAAAATTCAGCATATTTTGTAATTCATCAGGAGACATATTCCCA ATCATGTCAGATGCCATCTTAAACATCTCAGGAGACATTTCTGGCATATTAGGTCCCAAATTTG GAGGACTGGAGCTTGTTCCGTGCAACGAAGAAGCAGCTTCAAACATTTTCTGAAGTTCTTCTGG TTTCATTGTGCCAATCATATCGCTGGCAGTTTTTACTAATTCAGGTGACATTCCCTGCATTCCC AGCTTCGATAAGCCATTAGCATCTATGCTTGAAGACCCTGAAGGTCTAGAGTTCCCTTCTTCAG GAGGTTGTGAAACAGTGTATCCAGGGGATGAACTTCTTTGACTGCTTGATGGCTCAGAAGCATC ATCTACAACTTCTTCAATGACAACAC
Genomic sequence	CATTCTATCCAAAGCCTCAGGAGATAATGAAGACAAGGCTTCTTGAGCTTTGGCAGCATCCTCC TTCGTCATTTTCATTCCAAACTGCTCACTCATGCTTGACATCATCTCTGGACTGATGTTCTTCA TCATGGATGCATACATCTGTTGATTTAGAAAAAGACTGTTAAAATCCAAAGCCTACCATTTTTT GCTAGGTAACAAGTTCATTCAAAATTTCCTTCTGTTCGAAGATTCTATACCTTCCGCATCGCAG GATCTTTCATAGCATTTCTCATGGTTTCCTGCATATCAGCTGTAGAAGGTGCACCAGGGGATGA TGATTCACCCATCCTTTGGTTGCTTAATATTTCATGAGGATTTTCCATGACATTTTGAGAAGAT GAAGGTTGGCTGTTATCCACTGATCTCTGGGAAGTACTACTTGTTGTGGCTCTTGATGAAGATT GAAGCTTATTCTCTGGTCTCAAGGGGGAAGCACTAGGTCCACCCATATTAGAAGCAAAATTCAG CATATTTTGTAATTCATCAGGAGACATATTCCCAATCATGTCAGATGCCATCTTAAACATCTCA GGAGACATTTCTGGCATATTAGGTCCCAAATTTGGAGGACTGGAGCTTGTTCCGTGCAACGAAG AAGCAGCTTCAAACATTTTCTGAAGTTCTTCTGGTTTCATTGTGCCAATCATATCGCTGGCAGT TTTTACTAATTCAGGTGACATTCCCTGCATTCCCAGCTTCGATAAGCCATTAGCATCTATGCTT GAAGACCCTGAAGGTCTAGAGTTCCCTTCTTCAGGAGGTTGTGAAACAGTGTATCCAGGGGATG AACTTCTTTGACTGCTTGATGGCTCAGAAGCATCATCTACAACTTCTTCAATGACAACAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.253758203

Conservation Information
Conserved circRNAs	NA
PMCS	0.253758203

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							3272133-3271365(+)
3271310-3272131(-)
3271985-3272120(-)
						
						Potential amino acid sequence
						
							MTTPFYPKPQEIMKTRLLELWQHPPSSFSFQTAHSCLTSSLD*(+)
MLPKLKKPCLHYLLRLWIEWCCH*(-)
MQGMSPELVKTASDMIGTMKPEELQKMFEAASSLHGTSSSPPNLGPNMPEMSPEMFKMASDMIG
NMSPDELQNMLNFASNMGGPSASPLRPENKLQSSSRATTSSTSQRSVDNSQPSSSQNVMENPHE
ILSNQRMGESSSPGAPSTADMQETMRNAMKDPAMRKMYASMMKNISPEMMSSMSEQFGMKMTKE
DAAKAQEALSSLSPEALDRMVLSLKKL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	3272133-3271365(+) 3271310-3272131(-) 3271985-3272120(-)
Potential amino acid sequence	MTTPFYPKPQEIMKTRLLELWQHPPSSFSFQTAHSCLTSSLD(+) MLPKLKKPCLHYLLRLWIEWCCH(-) MQGMSPELVKTASDMIGTMKPEELQKMFEAASSLHGTSSSPPNLGPNMPEMSPEMFKMASDMIG NMSPDELQNMLNFASNMGGPSASPLRPENKLQSSSRATTSSTSQRSVDNSQPSSSQNVMENPHE ILSNQRMGESSSPGAPSTADMQETMRNAMKDPAMRKMYASMMKNISPEMMSSMSEQFGMKMTKE DAAKAQEALSSLSPEALDRMVLSLKKL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os07g0161000_circ_g.1

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