Plant Circular RNA Database

Detail information of Os05g0181100_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os05g0181100_circ_g.2
					
						ID in PlantcircBase
						osa_circ_026871
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 4880329-4881580  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os05g0181100						
					
						Parent gene annotation
						
							Glutamate dehydrogenase, NAD-specific domain containing protein.
 (Os05t0181100-00)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os05g0181100_circ_g.1 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0181000-01:4
Os05t0181100-00:4
Os05t0181000-01:4

General Information
CircRNA Name	Os05g0181100_circ_g.2
ID in PlantcircBase	osa_circ_026871
Alias	NA
Organism	Oryza sativa
Position	chr5: 4880329-4881580 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer
Parent gene	Os05g0181100
Parent gene annotation	Glutamate dehydrogenase, NAD-specific domain containing protein. (Os05t0181100-00)
Parent gene strand	-
Alternative splicing	Os05g0181100_circ_g.1
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0181000-01:4 Os05t0181100-00:4 Os05t0181000-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTCTATTAACCAGCTGGTACATTGCATCAACGGTTTTAAAGAGGTTGCATTGTCTAATAGCCAA
GCATTTGATCACATCGACATGTCAGAAAAACAGAAGatgctggaggaggaagtggatgttccgg
tagcaactacaaatgagacactaaaggatgatactaaaatggatacagatgatgcactaggtga
ccctgctt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 711
Transcript exons: 4880329-4880547,4880920-4881114,4881185-4881331,4881431-
4881580
ATGCTGGAGGAGGAAGTGGATGTTCCGGTAGCAACTACAAATGAGACACTAAAGGATGATACTA
AAATGGATACAGATGATGCACTAGGTGACCCTGCTTCTGGAACTGATGAGAACATGCAGGAATC
CAAATGTTCTGCAGATGCCACTCATGGTGCTGCAGAAAATGGGAAGCCAGATTCTGAGGAAATA
TCTGCTCCAATGGATACTGATGCCAAGGTTGAGCCATTGATAAAGAATGTGAAGAAAATTGATG
TTCCAGTTTCTGGATTGGTTTATGGTGCACTGGGGTCTGAAGAATTGGTGAAAGCTTCTGAGAA
TGAGTATGAAATGGCTCTTCAGGACAGAGTAATGGAAGAAACCAAGGAGAAAAAGAATGCTGTC
GAGGCTTATGTTTATGACATGCGTAACAAGCTCTATGACAAGTACAACGATTTTGTCATGTCTG
AGTACAAGGAAGGGTTTATTGCCAAGCTTCAGGAGGTTGAGGATTGGCTGTACGAAGATGGTGA
AGATGAGACAAAAGGGGTTTATATTGCAAAGCTGGAAGAACTTAAAAAGGTTGGTGATCCTATT
GAGATCCGCTACAAAGAGTGGGCTGAAAGAAGCTCTTCTATTAACCAGCTGGTACATTGCATCA
ACGGTTTTAAAGAGGTTGCATTGTCTAATAGCCAAGCATTTGATCACATCGACATGTCAGAAAA
ACAGAAG
						
					

						Genomic sequence
						
							ATGCTGGAGGAGGAAGTGGATGTTCCGGTAGCAACTACAAATGAGACACTAAAGGATGATACTA
AAATGGATACAGATGATGCACTAGGTGACCCTGCTTCTGGAACTGATGAGAACATGCAGGAATC
CAAATGTTCTGCAGATGCCACTCATGGTGCTGCAGAAAATGGGAAGCCAGATTCTGAGGAAATA
TCTGCTCCAATGGATACTGATGCCAAGGTATGGATCTACTGTTGGGTGCTGGCACACTTCAGCT
TTAGTGTATGTATGTAGTTGGCATATCTGTTGCAGTATGTTTTCAAATAACGATGTTCTATATT
GTCTTAATTTGGTGGTTTCCTATCGCCATGAATGGAATTCCCTCGTCATGGTAGTGTGTTCAGA
GCATTTGATAATCTCCTTTTGAGTTTTAGATACTGTGCTGATTCATAGCTTTGTAAGTGCTATC
TTGTGAAGTGAAGCACAACGAATATAATTTAGTTACTTATAACATAGGAACTTCATGCAAGTTG
TGATCTATCTTGTTTATGCAAATGAGAGCTGCCCATCTTTTTATGAGACATATTTTCTGAAATG
GTTTTTCTTTTTTAGGTTGAGCCATTGATAAAGAATGTGAAGAAAATTGATGTTCCAGTTTCTG
GATTGGTTTATGGTGCACTGGGGTCTGAAGAATTGGTGAAAGCTTCTGAGAATGAGTATGAAAT
GGCTCTTCAGGACAGAGTAATGGAAGAAACCAAGGAGAAAAAGAATGCTGTCGAGGCTTATGTT
TATGACATGCGTAACAAGGTATGCCGAGTTTAATTTTGTGATGATATATGAACATTTGAACAGT
ACCTGATGAGTTTCTTGCGTTCAGCTCTATGACAAGTACAACGATTTTGTCATGTCTGAGTACA
AGGAAGGGTTTATTGCCAAGCTTCAGGAGGTTGAGGATTGGCTGTACGAAGATGGTGAAGATGA
GACAAAAGGGGTTTATATTGCAAAGCTGGAAGAACTTAAAAAGGTACCTCTTTTATTGCACTTC
TTGGTTTACATTGTGTCTTTATGTGTTGGTTTGGTTTGTTCTTGGACATTGAGTTTTGATATGC
CTGAACTTTTTTAGGTTGGTGATCCTATTGAGATCCGCTACAAAGAGTGGGCTGAAAGAAGCTC
TTCTATTAACCAGCTGGTACATTGCATCAACGGTTTTAAAGAGGTTGCATTGTCTAATAGCCAA
GCATTTGATCACATCGACATGTCAGAAAAACAGAAG
						
					

			
		

Sequences
Splice junction sequence	TTCTATTAACCAGCTGGTACATTGCATCAACGGTTTTAAAGAGGTTGCATTGTCTAATAGCCAA GCATTTGATCACATCGACATGTCAGAAAAACAGAAGatgctggaggaggaagtggatgttccgg tagcaactacaaatgagacactaaaggatgatactaaaatggatacagatgatgcactaggtga ccctgctt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 711 Transcript exons: 4880329-4880547,4880920-4881114,4881185-4881331,4881431- 4881580 ATGCTGGAGGAGGAAGTGGATGTTCCGGTAGCAACTACAAATGAGACACTAAAGGATGATACTA AAATGGATACAGATGATGCACTAGGTGACCCTGCTTCTGGAACTGATGAGAACATGCAGGAATC CAAATGTTCTGCAGATGCCACTCATGGTGCTGCAGAAAATGGGAAGCCAGATTCTGAGGAAATA TCTGCTCCAATGGATACTGATGCCAAGGTTGAGCCATTGATAAAGAATGTGAAGAAAATTGATG TTCCAGTTTCTGGATTGGTTTATGGTGCACTGGGGTCTGAAGAATTGGTGAAAGCTTCTGAGAA TGAGTATGAAATGGCTCTTCAGGACAGAGTAATGGAAGAAACCAAGGAGAAAAAGAATGCTGTC GAGGCTTATGTTTATGACATGCGTAACAAGCTCTATGACAAGTACAACGATTTTGTCATGTCTG AGTACAAGGAAGGGTTTATTGCCAAGCTTCAGGAGGTTGAGGATTGGCTGTACGAAGATGGTGA AGATGAGACAAAAGGGGTTTATATTGCAAAGCTGGAAGAACTTAAAAAGGTTGGTGATCCTATT GAGATCCGCTACAAAGAGTGGGCTGAAAGAAGCTCTTCTATTAACCAGCTGGTACATTGCATCA ACGGTTTTAAAGAGGTTGCATTGTCTAATAGCCAAGCATTTGATCACATCGACATGTCAGAAAA ACAGAAG
Genomic sequence	ATGCTGGAGGAGGAAGTGGATGTTCCGGTAGCAACTACAAATGAGACACTAAAGGATGATACTA AAATGGATACAGATGATGCACTAGGTGACCCTGCTTCTGGAACTGATGAGAACATGCAGGAATC CAAATGTTCTGCAGATGCCACTCATGGTGCTGCAGAAAATGGGAAGCCAGATTCTGAGGAAATA TCTGCTCCAATGGATACTGATGCCAAGGTATGGATCTACTGTTGGGTGCTGGCACACTTCAGCT TTAGTGTATGTATGTAGTTGGCATATCTGTTGCAGTATGTTTTCAAATAACGATGTTCTATATT GTCTTAATTTGGTGGTTTCCTATCGCCATGAATGGAATTCCCTCGTCATGGTAGTGTGTTCAGA GCATTTGATAATCTCCTTTTGAGTTTTAGATACTGTGCTGATTCATAGCTTTGTAAGTGCTATC TTGTGAAGTGAAGCACAACGAATATAATTTAGTTACTTATAACATAGGAACTTCATGCAAGTTG TGATCTATCTTGTTTATGCAAATGAGAGCTGCCCATCTTTTTATGAGACATATTTTCTGAAATG GTTTTTCTTTTTTAGGTTGAGCCATTGATAAAGAATGTGAAGAAAATTGATGTTCCAGTTTCTG GATTGGTTTATGGTGCACTGGGGTCTGAAGAATTGGTGAAAGCTTCTGAGAATGAGTATGAAAT GGCTCTTCAGGACAGAGTAATGGAAGAAACCAAGGAGAAAAAGAATGCTGTCGAGGCTTATGTT TATGACATGCGTAACAAGGTATGCCGAGTTTAATTTTGTGATGATATATGAACATTTGAACAGT ACCTGATGAGTTTCTTGCGTTCAGCTCTATGACAAGTACAACGATTTTGTCATGTCTGAGTACA AGGAAGGGTTTATTGCCAAGCTTCAGGAGGTTGAGGATTGGCTGTACGAAGATGGTGAAGATGA GACAAAAGGGGTTTATATTGCAAAGCTGGAAGAACTTAAAAAGGTACCTCTTTTATTGCACTTC TTGGTTTACATTGTGTCTTTATGTGTTGGTTTGGTTTGTTCTTGGACATTGAGTTTTGATATGC CTGAACTTTTTTAGGTTGGTGATCCTATTGAGATCCGCTACAAAGAGTGGGCTGAAAGAAGCTC TTCTATTAACCAGCTGGTACATTGCATCAACGGTTTTAAAGAGGTTGCATTGTCTAATAGCCAA GCATTTGATCACATCGACATGTCAGAAAAACAGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.255521565

Conservation Information
Conserved circRNAs	NA
PMCS	0.255521565

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4880330-4881577(+)
4881078-4880356(+)
4880449-4881555(-)
						
						Potential amino acid sequence
						
							MLEEEVDVPVATTNETLKDDTKMDTDDALGDPASGTDENMQESKCSADATHGAAENGKPDSEEI
SAPMDTDAKVEPLIKNVKKIDVPVSGLVYGALGSEELVKASENEYEMALQDRVMEETKEKKNAV
EAYVYDMRNKLYDKYNDFVMSEYKEGFIAKLQEVEDWLYEDGEDETKGVYIAKLEELKKVGDPI
EIRYKEWAERSSSINQLVHCINGFKEVALSNSQAFDHIDMSEKQKMLEEEVDVPVATTNETLKD
DTKMDTDDALGDPASGTDENMQESKCSADATHGAAENGKPDSEEISAPMDTDAKVEPLIKNVKK
IDVPVSGLVYGALGSEELVKASENEYEMALQDRVMEETKEKKNAVEAYVYDMRNKLYDKYNDFV
MSEYKEGFIAKLQEVEDWLYEDGEDETKGVYIAKLEELKKVGDPIEIRYKEWAERSSSINQLVH
CINGFKEVALSNSQAFDHIDMSEKQKMLEEEVDVPVATTNETLKDDTKMDTDDALGDPASGTDE
NMQESKCSADATHGAAENGKPDSEEISAPMDTDAKVEPLIKNVKKIDVPVSGLVYGALGSEELV
KASENEYEMALQDRVMEETKEKKNAVEAYVYDMRNKLYDKYNDFVMSEYKEGFIAKLQEVEDWL
YEDGEDETKGVYIAKLEELKKVGDPIEIRYKEWAERSSSINQLVHCINGFKEVALSNSQAFDHI
DMSEKQKMLEEEVDVPVATTNETLKDDTKMDTDDALGDPASGTDENMQESKCSADATHGAAENG
KPDSEEISAPMDTDAKVEPLIKNVKKIDVPVSGLVYGALGSEELVKASENEYEMALQDRVMEET
KEKKNAVEAYVYDMRNKLYDKYNDFVMSEYKEGFIAKLQEVEDWLYEDGEDETKGVYIAKLEEL
KKVGDPIEIRYKEWAERSSSINQLVHCINGFKEVALSNSQAFDHIDMSEKQK(+)
MLSRLMFMTCVTSSMTSTTILSCLSTRKGLLPSFRRLRIGCTKMVKMRQKGFILQSWKNLKRLV
ILLRSATKSGLKEALLLTSWYIASTVLKRLHCLIAKHLITSTCQKNRRCWRRKWMFR*(+)
MFSSVPEAGSPSASSVSILVSSFSVSFVVATGTSTSSSSIFCFSDMSM*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	4880330-4881577(+) 4881078-4880356(+) 4880449-4881555(-)
Potential amino acid sequence	MLEEEVDVPVATTNETLKDDTKMDTDDALGDPASGTDENMQESKCSADATHGAAENGKPDSEEI SAPMDTDAKVEPLIKNVKKIDVPVSGLVYGALGSEELVKASENEYEMALQDRVMEETKEKKNAV EAYVYDMRNKLYDKYNDFVMSEYKEGFIAKLQEVEDWLYEDGEDETKGVYIAKLEELKKVGDPI EIRYKEWAERSSSINQLVHCINGFKEVALSNSQAFDHIDMSEKQKMLEEEVDVPVATTNETLKD DTKMDTDDALGDPASGTDENMQESKCSADATHGAAENGKPDSEEISAPMDTDAKVEPLIKNVKK IDVPVSGLVYGALGSEELVKASENEYEMALQDRVMEETKEKKNAVEAYVYDMRNKLYDKYNDFV MSEYKEGFIAKLQEVEDWLYEDGEDETKGVYIAKLEELKKVGDPIEIRYKEWAERSSSINQLVH CINGFKEVALSNSQAFDHIDMSEKQKMLEEEVDVPVATTNETLKDDTKMDTDDALGDPASGTDE NMQESKCSADATHGAAENGKPDSEEISAPMDTDAKVEPLIKNVKKIDVPVSGLVYGALGSEELV KASENEYEMALQDRVMEETKEKKNAVEAYVYDMRNKLYDKYNDFVMSEYKEGFIAKLQEVEDWL YEDGEDETKGVYIAKLEELKKVGDPIEIRYKEWAERSSSINQLVHCINGFKEVALSNSQAFDHI DMSEKQKMLEEEVDVPVATTNETLKDDTKMDTDDALGDPASGTDENMQESKCSADATHGAAENG KPDSEEISAPMDTDAKVEPLIKNVKKIDVPVSGLVYGALGSEELVKASENEYEMALQDRVMEET KEKKNAVEAYVYDMRNKLYDKYNDFVMSEYKEGFIAKLQEVEDWLYEDGEDETKGVYIAKLEEL KKVGDPIEIRYKEWAERSSSINQLVHCINGFKEVALSNSQAFDHIDMSEKQK(+) MLSRLMFMTCVTSSMTSTTILSCLSTRKGLLPSFRRLRIGCTKMVKMRQKGFILQSWKNLKRLV ILLRSATKSGLKEALLLTSWYIASTVLKRLHCLIAKHLITSTCQKNRRCWRRKWMFR(+) MFSSVPEAGSPSASSVSILVSSFSVSFVVATGTSTSSSSIFCFSDMSM(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0181100_circ_g.2

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