Plant Circular RNA Database

Detail information of Os08g0295300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os08g0295300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_036613
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 11893832-11895699  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os08g0295300						
					
						Parent gene annotation
						
							Similar to Threonyl-tRNA synthetase, cytoplasmic (EC 6.1.1.3) (T
hreonine--tRNA ligase) (ThrRS). (Os08t0295300-01);Similar to Thr
eonyl-tRNA synthetase (Fragment). (Os08t0295300-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os08g0295300_circ_g.2 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0295300-01:7
Os08t0295300-02:4

General Information
CircRNA Name	Os08g0295300_circ_g.1
ID in PlantcircBase	osa_circ_036613
Alias	NA
Organism	Oryza sativa
Position	chr8: 11893832-11895699 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os08g0295300
Parent gene annotation	Similar to Threonyl-tRNA synthetase, cytoplasmic (EC 6.1.1.3) (T hreonine--tRNA ligase) (ThrRS). (Os08t0295300-01);Similar to Thr eonyl-tRNA synthetase (Fragment). (Os08t0295300-02)
Parent gene strand	-
Alternative splicing	Os08g0295300_circ_g.2
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0295300-01:7 Os08t0295300-02:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							AACAAGCATAAATCAGGAATTTTGCCTTAAATTTATTTTCTGCGAACATTTCAAGGGCCTCTGC
CCTGGAGACCTCAATTCGTTCAAATGGTTGTTTTTCctctgtatgaacgaaccctgtgctcaaa
cattaggcaatggccaggacaattcattggcttgagaccaaattcctgtttctcaatctgcaag
aataagac

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CTCTGTATGAACGAACCCTGTGCTCAAACATTAGGCAATGGCCAGGACAATTCATTGGCTTGAG
ACCAAATTCCTGTTTCTCAATCTGCAAGAATAAGACCAACAAGCACCCAGTAATAAAGATCGTA
ATTCATCCAAAATGTAATCTATAATATGTACAGAGCAAATCCTCTGCACACTAGACTTACCTCA
AAAACAAACATATTCTCCTTGTAGTTTGCAGCATGTCCAGAGGTTTCCCAGAGTTGCATATTGT
AAATATTTGGGCTCAAAACCTGAAAATACAGACATCATGAACAGAGAGAAACAGTGGAAATGCA
TGCCCATGATCTAGTAATCTAATCATAATCTGAACAAAAGATTGACCAGCCAATCAAATTAATG
CCCACCTCTTGATATCCTCTATCTCTGTATTGCTGTCGCAAAAAATCCATCAATTTGTTGTATA
TTATAGCACCATGTGGAAGGAAGAAGCAGCTTCCTGGACTGCACAGTCAAAGTGAATCAAACAT
CCTGAGGCAGAATAGAAGCCAAGGTAAATTAAGATATGAAAAAGTCAAACCGAAAACCTTGAGT
TACCTAAGTTGATGGAAAAAGAAGAGATCCTGGGTCTGTCCTAATAGCCTATGATCACGCTTCT
TAGCCTCTTCTAGCAGATGTTTATATTCCTATGGATTATTGAGCAGGAAACATATATGAAAACC
ATAGTGTACCAGTGATAAAACAAATGGATAAAAAAGAACTTAGGAATTAGTAACTTGTAAAATA
GCTGACAAGGGTTGCACTGTCTGAACCTAACCATGTATTAATTGAAGAAATTCGTAATAACTGA
CTAATAAAAAGAAACCATATACTTCTGTAATGACAATTGTTGTTCAGTTGGGCAAATCAACCCC
AACCTCCACCAAAGCACAGAGCCCATAGATACACTTTTGAGATATAGTTGTATATTTTGTGCAA
TTTACATGCCATGTAGTGCACGCTAAACTTCCTGCCAAATCAACTCCAATCTTATCACAATGAA
ATATTAATACTCTAGTGTCTAAAATGCAAACATAGAGGGTACATTATTGTGTCCAGTAGCACAG
ACATGATAGCACAAAAACAGACTCCACATTAATTAATGAACGCCCATATCTGAACAATAGCCAA
AATCATATGCTATACCTTGAGACGTTTAGAATCAGGAAAAGAAATTCCATATACTCTCTGCAGG
CTCTCTCGATCTGCTTTCCCTCTCCAATACGACGATGATGCCTACATATTTACATGCAAGTACA
AATTGGATAGTTCCATAAAAATATTGGCAAGCAAAAGCTTAAGACAACCAAAGAATTACCTTAA
GACAAGCAAAAGCTTTAACAAAGGAAGTATTGGGGATGTGTGGCCCACGGCAAAGGTCAACTAG
AGGACCACATCTGTATACTGTAATGGTCTTGTCTTCAGGCAACTCATTAATGATTTCAACCTAG
AAACACAATTCAAACAAATAAAAAATTTAATAAAATCTCAAATGTGATATAAGCAGAAATAGAT
ATTTAGGGCCTCATTAATTCGCTTATGCTTATGCTTATAAGTCAAGTTTTGAAATTTAGAACTT
AATTTAATTTAATTTTTTTCTTGTTTCTTTCATTATAGTTTATTTTACAGCATTTTCTTTTGAG
TCGTTTTAGTCACAAATGATTTTTTTTGTTTGTTAATAAGCAATTCGGATAAGCATATGAATAA
GCGAAACAATGGGACTGTTAGAGTACAAGAAAAGATCAAGAACAAGCATAAATCAGGAATTTTG
CCTTAAATTTATTTTCTGCGAACATTTCAAGGGCCTCTGCCCTGGAGACCTCAATTCGTTCAAA
TGGTTGTTTTTC
						
					

			
		

Sequences
Splice junction sequence	AACAAGCATAAATCAGGAATTTTGCCTTAAATTTATTTTCTGCGAACATTTCAAGGGCCTCTGC CCTGGAGACCTCAATTCGTTCAAATGGTTGTTTTTCctctgtatgaacgaaccctgtgctcaaa cattaggcaatggccaggacaattcattggcttgagaccaaattcctgtttctcaatctgcaag aataagac
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CTCTGTATGAACGAACCCTGTGCTCAAACATTAGGCAATGGCCAGGACAATTCATTGGCTTGAG ACCAAATTCCTGTTTCTCAATCTGCAAGAATAAGACCAACAAGCACCCAGTAATAAAGATCGTA ATTCATCCAAAATGTAATCTATAATATGTACAGAGCAAATCCTCTGCACACTAGACTTACCTCA AAAACAAACATATTCTCCTTGTAGTTTGCAGCATGTCCAGAGGTTTCCCAGAGTTGCATATTGT AAATATTTGGGCTCAAAACCTGAAAATACAGACATCATGAACAGAGAGAAACAGTGGAAATGCA TGCCCATGATCTAGTAATCTAATCATAATCTGAACAAAAGATTGACCAGCCAATCAAATTAATG CCCACCTCTTGATATCCTCTATCTCTGTATTGCTGTCGCAAAAAATCCATCAATTTGTTGTATA TTATAGCACCATGTGGAAGGAAGAAGCAGCTTCCTGGACTGCACAGTCAAAGTGAATCAAACAT CCTGAGGCAGAATAGAAGCCAAGGTAAATTAAGATATGAAAAAGTCAAACCGAAAACCTTGAGT TACCTAAGTTGATGGAAAAAGAAGAGATCCTGGGTCTGTCCTAATAGCCTATGATCACGCTTCT TAGCCTCTTCTAGCAGATGTTTATATTCCTATGGATTATTGAGCAGGAAACATATATGAAAACC ATAGTGTACCAGTGATAAAACAAATGGATAAAAAAGAACTTAGGAATTAGTAACTTGTAAAATA GCTGACAAGGGTTGCACTGTCTGAACCTAACCATGTATTAATTGAAGAAATTCGTAATAACTGA CTAATAAAAAGAAACCATATACTTCTGTAATGACAATTGTTGTTCAGTTGGGCAAATCAACCCC AACCTCCACCAAAGCACAGAGCCCATAGATACACTTTTGAGATATAGTTGTATATTTTGTGCAA TTTACATGCCATGTAGTGCACGCTAAACTTCCTGCCAAATCAACTCCAATCTTATCACAATGAA ATATTAATACTCTAGTGTCTAAAATGCAAACATAGAGGGTACATTATTGTGTCCAGTAGCACAG ACATGATAGCACAAAAACAGACTCCACATTAATTAATGAACGCCCATATCTGAACAATAGCCAA AATCATATGCTATACCTTGAGACGTTTAGAATCAGGAAAAGAAATTCCATATACTCTCTGCAGG CTCTCTCGATCTGCTTTCCCTCTCCAATACGACGATGATGCCTACATATTTACATGCAAGTACA AATTGGATAGTTCCATAAAAATATTGGCAAGCAAAAGCTTAAGACAACCAAAGAATTACCTTAA GACAAGCAAAAGCTTTAACAAAGGAAGTATTGGGGATGTGTGGCCCACGGCAAAGGTCAACTAG AGGACCACATCTGTATACTGTAATGGTCTTGTCTTCAGGCAACTCATTAATGATTTCAACCTAG AAACACAATTCAAACAAATAAAAAATTTAATAAAATCTCAAATGTGATATAAGCAGAAATAGAT ATTTAGGGCCTCATTAATTCGCTTATGCTTATGCTTATAAGTCAAGTTTTGAAATTTAGAACTT AATTTAATTTAATTTTTTTCTTGTTTCTTTCATTATAGTTTATTTTACAGCATTTTCTTTTGAG TCGTTTTAGTCACAAATGATTTTTTTTGTTTGTTAATAAGCAATTCGGATAAGCATATGAATAA GCGAAACAATGGGACTGTTAGAGTACAAGAAAAGATCAAGAACAAGCATAAATCAGGAATTTTG CCTTAAATTTATTTTCTGCGAACATTTCAAGGGCCTCTGCCCTGGAGACCTCAATTCGTTCAAA TGGTTGTTTTTC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.262964173

Conservation Information
Conserved circRNAs	NA
PMCS	0.262964173

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							11895688-11894044(+)
11895212-11893838(+)
11895650-11895685(-)
						
						Potential amino acid sequence
						
							MVVFPLYERTLCSNIRQWPGQFIGLRPNSCFSISKTNIFSL*(+)
MCGPRQRSTRGPHLYTVMVLSSGNSLMISTLNLFSANISRASALETSIRSNGCFSSV*(+)
MFAENKFKVEIINELPEDKTITVYRCGPLVDLCRGPHIPNTSFVKAFACLKASSSYWRGKADRE
SLQRVYGISFPDSKRLKEYKHLLEEAKKRDHRLLGQTQDLFFFHQLSPGSCFFLPHGAIIYNKL
MDFLRQQYRDRGYQEVLSPNIYNMQLWETSGHAANYKENMFVFEIEKQEFGLKPMNCPGHCLMF
EHRVRSYRGKTTI*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	11895688-11894044(+) 11895212-11893838(+) 11895650-11895685(-)
Potential amino acid sequence	MVVFPLYERTLCSNIRQWPGQFIGLRPNSCFSISKTNIFSL(+) MCGPRQRSTRGPHLYTVMVLSSGNSLMISTLNLFSANISRASALETSIRSNGCFSSV(+) MFAENKFKVEIINELPEDKTITVYRCGPLVDLCRGPHIPNTSFVKAFACLKASSSYWRGKADRE SLQRVYGISFPDSKRLKEYKHLLEEAKKRDHRLLGQTQDLFFFHQLSPGSCFFLPHGAIIYNKL MDFLRQQYRDRGYQEVLSPNIYNMQLWETSGHAANYKENMFVFEIEKQEFGLKPMNCPGHCLMF EHRVRSYRGKTTI*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os08g0295300_circ_g.1

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