Plant Circular RNA Database

Detail information of Os05g0495300_circ_g.14

			
						 General Information
					
						CircRNA Name
						Os05g0495300_circ_g.14
					
						ID in PlantcircBase
						osa_circ_028373
					
						Alias
						Os05circ14557
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 24328245-24328410  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						SMALT, Segemehl, circRNA_finder, find_circ
					
						Parent gene
						
							Os05g0495300						
					
						Parent gene annotation
						
							Conserved hypothetical protein. (Os05t0495300-01);Hypothetical c
onserved gene. (Os05t0495300-02);Conserved hypothetical protein.
 (Os05t0495300-06);Non-protein coding transcript. (Os05t0495300-
07)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os05g0495300_circ_g.8 Os05g0495300_circ_g.9 Os05g0495300_circ_g.10 Os05g0495300_circ_g.11 Os05g0495300_circ_g.12 Os05g0495300_circ_g.13 Os05g0495300_circ_g.15 Os05g0495300_circ_g.16 Os05g0495300_circ_g.17 Os05g0495300_circ_g.18 						
					
						Support reads
						2/2
					
						Tissues
						panicle/seed
					
						Exon boundary  
						
							Yes-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0495300-06:1
Os05t0495300-01:1
Os05t0495300-02:1

General Information
CircRNA Name	Os05g0495300_circ_g.14
ID in PlantcircBase	osa_circ_028373
Alias	Os05circ14557
Organism	Oryza sativa
Position	chr5: 24328245-24328410 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	SMALT, Segemehl, circRNA_finder, find_circ
Parent gene	Os05g0495300
Parent gene annotation	Conserved hypothetical protein. (Os05t0495300-01);Hypothetical c onserved gene. (Os05t0495300-02);Conserved hypothetical protein. (Os05t0495300-06);Non-protein coding transcript. (Os05t0495300- 07)
Parent gene strand	-
Alternative splicing	Os05g0495300_circ_g.8 Os05g0495300_circ_g.9 Os05g0495300_circ_g.10 Os05g0495300_circ_g.11 Os05g0495300_circ_g.12 Os05g0495300_circ_g.13 Os05g0495300_circ_g.15 Os05g0495300_circ_g.16 Os05g0495300_circ_g.17 Os05g0495300_circ_g.18
Support reads	2/2
Tissues	panicle/seed
Exon boundary	Yes-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0495300-06:1 Os05t0495300-01:1 Os05t0495300-02:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTCTAATGATGAATTGAAGAGAAGGGTGGTGATTTAAAGATTTCACTCGGTGAAGGTTGGCCTT
GCAAAATTATGAGATAGGCctgcagattggccatagaaattatgcagaaacctgcagtgagcaa
tagattggacttaaatatatctttgtgaggtatataac

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 166
Transcript exons: 24328245-24328410
CTGCAGATTGGCCATAGAAATTATGCAGAAACCTGCAGTGAGCAATAGATTGGACTTAAATATA
TCTTTGTGAGGTATATAACTTCTAATGATGAATTGAAGAGAAGGGTGGTGATTTAAAGATTTCA
CTCGGTGAAGGTTGGCCTTGCAAAATTATGAGATAGGC
						
						Genomic sequence
						
							CTGCAGATTGGCCATAGAAATTATGCAGAAACCTGCAGTGAGCAATAGATTGGACTTAAATATA
TCTTTGTGAGGTATATAACTTCTAATGATGAATTGAAGAGAAGGGTGGTGATTTAAAGATTTCA
CTCGGTGAAGGTTGGCCTTGCAAAATTATGAGATAGGC

Sequences
Splice junction sequence	TTCTAATGATGAATTGAAGAGAAGGGTGGTGATTTAAAGATTTCACTCGGTGAAGGTTGGCCTT GCAAAATTATGAGATAGGCctgcagattggccatagaaattatgcagaaacctgcagtgagcaa tagattggacttaaatatatctttgtgaggtatataac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 166 Transcript exons: 24328245-24328410 CTGCAGATTGGCCATAGAAATTATGCAGAAACCTGCAGTGAGCAATAGATTGGACTTAAATATA TCTTTGTGAGGTATATAACTTCTAATGATGAATTGAAGAGAAGGGTGGTGATTTAAAGATTTCA CTCGGTGAAGGTTGGCCTTGCAAAATTATGAGATAGGC
Genomic sequence	CTGCAGATTGGCCATAGAAATTATGCAGAAACCTGCAGTGAGCAATAGATTGGACTTAAATATA TCTTTGTGAGGTATATAACTTCTAATGATGAATTGAAGAGAAGGGTGGTGATTTAAAGATTTCA CTCGGTGAAGGTTGGCCTTGCAAAATTATGAGATAGGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_006319 osi_circ_015610 osi_circ_015609 osi_circ_006318 
						
						PMCS
						0.452564103

Conservation Information
Conserved circRNAs	osi_circ_006319 osi_circ_015610 osi_circ_015609 osi_circ_006318
PMCS	0.452564103

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015;Chu et al., 2017

circRNA

Detail information of Os05g0495300_circ_g.14

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