Plant Circular RNA Database

Detail information of Os01g0617700_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os01g0617700_circ_g.3
					
						ID in PlantcircBase
						osa_circ_002657
					
						Alias
						Os_ciR954
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 24563762-24564524  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os01g0617700						
					
						Parent gene annotation
						
							Similar to CENP-C (Fragment). (Os01t0617700-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0617700_circ_g.1 Os01g0617700_circ_g.2 Os01g0617700_circ_g.4 Os01g0617700_circ_g.5 Os01g0617700_circ_g.6 Os01g0617700_circ_g.7 Os01g0617700_circ_g.8 Os01g0617700_circ_g.9 Os01g0617700_circ_g.10 						
					
						Support reads
						40
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0617700-01:2

General Information
CircRNA Name	Os01g0617700_circ_g.3
ID in PlantcircBase	osa_circ_002657
Alias	Os_ciR954
Organism	Oryza sativa
Position	chr1: 24563762-24564524 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	Os01g0617700
Parent gene annotation	Similar to CENP-C (Fragment). (Os01t0617700-01)
Parent gene strand	+
Alternative splicing	Os01g0617700_circ_g.1 Os01g0617700_circ_g.2 Os01g0617700_circ_g.4 Os01g0617700_circ_g.5 Os01g0617700_circ_g.6 Os01g0617700_circ_g.7 Os01g0617700_circ_g.8 Os01g0617700_circ_g.9 Os01g0617700_circ_g.10
Support reads	40
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0617700-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCACATTTTCTTCACAGATAATCCGATCCATACATCAGAAACACCCCCAATGGATACTAATCCA
CAGAATCAATCTGAAATACATCGTGGAAGCATTGAGaaactggcagttgataaaagcaacgcac
tctccccaagtcaagggaagcaacatagaggcaaaaataagaagcagccatcgaagagaggaaa
aagagaga

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 186
Transcript exons: 24563762-24563864,24564442-24564524
AAACTGGCAGTTGATAAAAGCAACGCACTCTCCCCAAGTCAAGGGAAGCAACATAGAGGCAAAA
ATAAGAAGCAGCCATCGAAGAGAGGAAAAAGAGAGACAGATAATCCGATCCATACATCAGAAAC
ACCCCCAATGGATACTAATCCACAGAATCAATCTGAAATACATCGTGGAAGCATTGAG
						
						Genomic sequence
						
							AAACTGGCAGTTGATAAAAGCAACGCACTCTCCCCAAGTCAAGGGAAGCAACATAGAGGCAAAA
ATAAGAAGCAGCCATCGAAGAGAGGAAAAAGAGAGACAGGTTTGTACCTATTCTTGTTTGGGCT
TATATTTAGTAACTTGTAATACATGTATGTTGAAGAATTGTTTTGACCTACTATCCAAATAGCT
ATGGCCACAAAGGACTATTATCCAAATAGATGTTTGGCCACAAAGGACTGTTCTTACCTATGGT
TGGCTGCTTCACTGGGCATTATCGTGCTCGTTACTTTGCAGACTCTAAATTACAAACATTTGAA
AACATAGGAAATAACCATAAAATGTCAGCAAAAACATTTCTATTACAATCTAATAAAGTTTCTC
TTATTCCTCTTATCTGTTCTATTGCTTTCATCTCTTATCTACAAACTCAACCGTGGTATAGCTG
AGAAGACATTGCAATAGGATATACTTTCGTCAAGCGCTAGTATCTCACTTCTTTTGGAAATTTG
TTATGTTGTGATATCAGAGTGGACTTTGCAAGGAGTACCTTATGGGGACATAAATGGCACAAAA
TAAGCTGTTTTTCTTTATTTCTTCAAACCAGAACAACTGAGAATTTTACTTTCCAATTTCTCAT
TTCTTATTCTTTTGAGGTTTAAAGCACATTTTCTTCACAGATAATCCGATCCATACATCAGAAA
CACCCCCAATGGATACTAATCCACAGAATCAATCTGAAATACATCGTGGAAGCATTGAG

Sequences
Splice junction sequence	GCACATTTTCTTCACAGATAATCCGATCCATACATCAGAAACACCCCCAATGGATACTAATCCA CAGAATCAATCTGAAATACATCGTGGAAGCATTGAGaaactggcagttgataaaagcaacgcac tctccccaagtcaagggaagcaacatagaggcaaaaataagaagcagccatcgaagagaggaaa aagagaga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 186 Transcript exons: 24563762-24563864,24564442-24564524 AAACTGGCAGTTGATAAAAGCAACGCACTCTCCCCAAGTCAAGGGAAGCAACATAGAGGCAAAA ATAAGAAGCAGCCATCGAAGAGAGGAAAAAGAGAGACAGATAATCCGATCCATACATCAGAAAC ACCCCCAATGGATACTAATCCACAGAATCAATCTGAAATACATCGTGGAAGCATTGAG
Genomic sequence	AAACTGGCAGTTGATAAAAGCAACGCACTCTCCCCAAGTCAAGGGAAGCAACATAGAGGCAAAA ATAAGAAGCAGCCATCGAAGAGAGGAAAAAGAGAGACAGGTTTGTACCTATTCTTGTTTGGGCT TATATTTAGTAACTTGTAATACATGTATGTTGAAGAATTGTTTTGACCTACTATCCAAATAGCT ATGGCCACAAAGGACTATTATCCAAATAGATGTTTGGCCACAAAGGACTGTTCTTACCTATGGT TGGCTGCTTCACTGGGCATTATCGTGCTCGTTACTTTGCAGACTCTAAATTACAAACATTTGAA AACATAGGAAATAACCATAAAATGTCAGCAAAAACATTTCTATTACAATCTAATAAAGTTTCTC TTATTCCTCTTATCTGTTCTATTGCTTTCATCTCTTATCTACAAACTCAACCGTGGTATAGCTG AGAAGACATTGCAATAGGATATACTTTCGTCAAGCGCTAGTATCTCACTTCTTTTGGAAATTTG TTATGTTGTGATATCAGAGTGGACTTTGCAAGGAGTACCTTATGGGGACATAAATGGCACAAAA TAAGCTGTTTTTCTTTATTTCTTCAAACCAGAACAACTGAGAATTTTACTTTCCAATTTCTCAT TTCTTATTCTTTTGAGGTTTAAAGCACATTTTCTTCACAGATAATCCGATCCATACATCAGAAA CACCCCCAATGGATACTAATCCACAGAATCAATCTGAAATACATCGTGGAAGCATTGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_010340 osi_circ_010341 osi_circ_010343 						
					
						PMCS
						0.401304183

Conservation Information
Conserved circRNAs	osi_circ_010340 osi_circ_010341 osi_circ_010343
PMCS	0.401304183

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							24564475-24564521(+)
24564457-24564461(-)
24564461-24564483(-)
						
						Potential amino acid sequence
						
							MDTNPQNQSEIHRGSIEKLAVDKSNALSPSQGKQHRGKNKKQPSKRGKRETDNPIHTSETPPMD
TNPQNQSEIHRGSIEKLAVDKSNALSPSQGKQHRGKNKKQPSKRGKRETDNPIHTSETPPMDTN
PQNQSEIHRGSIEKLAVDKSNALSPSQGKQHRGKNKKQPSKRGKRETDNPIHTSETPPMDTNPQ
NQSEIHRGSIE(+)
MDRIICLSFSSLRWLLLIFASMLLPLTWGECVAFINCQFLNASTMYFRLILWISIHWGCF*(-)
MYGSDYLSLFFLSSMAASYFCLYVASLDLGRVRCFYQLPVSQCFHDVFQIDSVD*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	24564475-24564521(+) 24564457-24564461(-) 24564461-24564483(-)
Potential amino acid sequence	MDTNPQNQSEIHRGSIEKLAVDKSNALSPSQGKQHRGKNKKQPSKRGKRETDNPIHTSETPPMD TNPQNQSEIHRGSIEKLAVDKSNALSPSQGKQHRGKNKKQPSKRGKRETDNPIHTSETPPMDTN PQNQSEIHRGSIEKLAVDKSNALSPSQGKQHRGKNKKQPSKRGKRETDNPIHTSETPPMDTNPQ NQSEIHRGSIE(+) MDRIICLSFSSLRWLLLIFASMLLPLTWGECVAFINCQFLNASTMYFRLILWISIHWGCF(-) MYGSDYLSLFFLSSMAASYFCLYVASLDLGRVRCFYQLPVSQCFHDVFQIDSVD(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os01g0617700_circ_g.3

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