Plant Circular RNA Database

Detail information of Os02g0709400_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0709400_circ_g.2
					
						ID in PlantcircBase
						osa_circ_016188
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 29342990-29350712  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os02g0709400						
					
						Parent gene annotation
						
							Similar to Dek1-calpain-like protein. (Os02t0709400-01);Similar 
to Dek1-calpain-like protein. (Os02t0709400-02);Similar to Dek1-
calpain-like protein. (Os02t0709400-03);Similar to Dek1-calpain-
like protein. (Os02t0709400-04)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0709400_circ_g.1 Os02g0709400_circ_g.3 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0709400-01:18
Os02t0709400-02:18

General Information
CircRNA Name	Os02g0709400_circ_g.2
ID in PlantcircBase	osa_circ_016188
Alias	NA
Organism	Oryza sativa
Position	chr2: 29342990-29350712 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os02g0709400
Parent gene annotation	Similar to Dek1-calpain-like protein. (Os02t0709400-01);Similar to Dek1-calpain-like protein. (Os02t0709400-02);Similar to Dek1- calpain-like protein. (Os02t0709400-03);Similar to Dek1-calpain- like protein. (Os02t0709400-04)
Parent gene strand	+
Alternative splicing	Os02g0709400_circ_g.1 Os02g0709400_circ_g.3
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0709400-01:18 Os02t0709400-02:18

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTAAATTAATTGTGAAATTTTTGTCAGATGCTGGAGGACGCTGTTCACTTTCTCAGTAAGGAT
ACAGTTGTACAAGCAATGTCACGTTCTGCTAACAAAgtttgtttgagcctgtatacatgatatc
agcttccagaaggtgattgcagagggggaggatggaagaggaggaacaccgcggggttgttctt
gtttgcag

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CTTAAATTAATTGTGAAATTTTTGTCAGATGCTGGAGGACGCTGTTCACTTTCTCAGTAAGGAT ACAGTTGTACAAGCAATGTCACGTTCTGCTAACAAAgtttgtttgagcctgtatacatgatatc agcttccagaaggtgattgcagagggggaggatggaagaggaggaacaccgcggggttgttctt gtttgcag
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.133214535

Conservation Information
Conserved circRNAs	NA
PMCS	0.133214535

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							29343050-29343010(+)
29343094-29350707(-)
						
						Potential amino acid sequence
						
							MEEEEHRGVVLVCSICGFLFAVLGPLSFWILWAVNWRPWRLYSWIYARKWPAYVQGPQLSTLCS
FFTLFAWLVVVSPITVLLVWGGILIALLERNIIGLAVIMVGVALLLSFYSIMLWWRTQWQSSKA
VAYLLLLAVGLLCAYEFCAVYVTTGASASELNSPSGFFFGVSAISLAINMLFISKILFNGSGFD
VDEYVRRLYKFAYSDCVEVAPVSCSPDPPDPSELYMTKSSRVLHLGLLYLCSLMVLVVYSILYG
LTSKEARWLGALTSVAVVILDWNLGLCSFRFELLKSRMIALFVAGTSRVFLICFGVHYWYLGHC
ISYAFVASVLLAAAVSCWLSISNPSVARIDALRSTVIKLREGFRRKGQTSSSNSSDGCGSSVKR
SSGSVEAGPHGNATDSMYRSNSQSDCVNWNNVPFDRSNSCQEGQSSDKNIDSGRASLAHRSNSC
LSAVAVQDPETAVVSADRHGDPTASLVVCSSSGLESQGCESSGSATASGNQQLLDLNLAAIFQD
RLNDPRITSMLKRNGGLGDVELANLLQDKGLDPNFSYMMKDKVMDPRILALLQRSSLDADREHQ
DDVDVTGTDSDRLDTTIANQISLSEELRRSGLENWLNLSRLMFHQVAGSPIRAFVVFTLIFIIE
TVTVAVHRPKPIKVINATHEQFEFGFSILLLSPVVCSIMAFIWSLCAEEMTMTSKPRKYGFIAW
LLSTCVGLLLSFLSKSSVILGLSLTVPLMVACLSFAIPIWMRNGYRFWIPGGELDSRENIRQAP
GKKERALFAISITVFTASVIGLGAIVSAKPLDALGYKGWDADKKSFYSPYATSMYLGWALSSTI
AVLATGVIPIVAWFATYRFSPSSAICVGLFATVLVSFCGVSYWGVVNSRQDGVPLKADFLAALL
PLLCIPAVFSLFTGMYKWKDDDWKISRGVYLFVGMGVLLLLGAISAVIVTIRPWTVGVACLLVI
LFLVFAIGVIHYWTSNNFYLTRTQMLLVCSLAFLLALAAFLMGLFQEKPFVGASIGYFSFLFLL
TGRALTVLLSPPIVVYSPRVLPVYVYDAHADSAKNVSYAFLILYGIALATEVWGVIASLILNPP
FIGAAISAITLVIAFSFAVSRPCLTLKMLEDAVHFLSKDTVVQAMSRSANKVCLSLYT*(+)
MLQTRTTPRCSSSSILPLCNHLLEADIMYTGSNKLC*(-)
						
						Sponge-miRNAs
						
							osa-miR5508 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	29343050-29343010(+) 29343094-29350707(-)
Potential amino acid sequence	MEEEEHRGVVLVCSICGFLFAVLGPLSFWILWAVNWRPWRLYSWIYARKWPAYVQGPQLSTLCS FFTLFAWLVVVSPITVLLVWGGILIALLERNIIGLAVIMVGVALLLSFYSIMLWWRTQWQSSKA VAYLLLLAVGLLCAYEFCAVYVTTGASASELNSPSGFFFGVSAISLAINMLFISKILFNGSGFD VDEYVRRLYKFAYSDCVEVAPVSCSPDPPDPSELYMTKSSRVLHLGLLYLCSLMVLVVYSILYG LTSKEARWLGALTSVAVVILDWNLGLCSFRFELLKSRMIALFVAGTSRVFLICFGVHYWYLGHC ISYAFVASVLLAAAVSCWLSISNPSVARIDALRSTVIKLREGFRRKGQTSSSNSSDGCGSSVKR SSGSVEAGPHGNATDSMYRSNSQSDCVNWNNVPFDRSNSCQEGQSSDKNIDSGRASLAHRSNSC LSAVAVQDPETAVVSADRHGDPTASLVVCSSSGLESQGCESSGSATASGNQQLLDLNLAAIFQD RLNDPRITSMLKRNGGLGDVELANLLQDKGLDPNFSYMMKDKVMDPRILALLQRSSLDADREHQ DDVDVTGTDSDRLDTTIANQISLSEELRRSGLENWLNLSRLMFHQVAGSPIRAFVVFTLIFIIE TVTVAVHRPKPIKVINATHEQFEFGFSILLLSPVVCSIMAFIWSLCAEEMTMTSKPRKYGFIAW LLSTCVGLLLSFLSKSSVILGLSLTVPLMVACLSFAIPIWMRNGYRFWIPGGELDSRENIRQAP GKKERALFAISITVFTASVIGLGAIVSAKPLDALGYKGWDADKKSFYSPYATSMYLGWALSSTI AVLATGVIPIVAWFATYRFSPSSAICVGLFATVLVSFCGVSYWGVVNSRQDGVPLKADFLAALL PLLCIPAVFSLFTGMYKWKDDDWKISRGVYLFVGMGVLLLLGAISAVIVTIRPWTVGVACLLVI LFLVFAIGVIHYWTSNNFYLTRTQMLLVCSLAFLLALAAFLMGLFQEKPFVGASIGYFSFLFLL TGRALTVLLSPPIVVYSPRVLPVYVYDAHADSAKNVSYAFLILYGIALATEVWGVIASLILNPP FIGAAISAITLVIAFSFAVSRPCLTLKMLEDAVHFLSKDTVVQAMSRSANKVCLSLYT(+) MLQTRTTPRCSSSSILPLCNHLLEADIMYTGSNKLC(-)
Sponge-miRNAs	osa-miR5508
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0709400_circ_g.2

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