Plant Circular RNA Database

Detail information of orai.001G115700_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.001G115700_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000059
					
						Alias
						Chr01:13724146|13725373
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr01: 13724146-13725373  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.001G115700						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.001G115700.2:4
Gorai.001G115700.1:4

General Information
CircRNA Name	orai.001G115700_circ_g.1
ID in PlantcircBase	gra_circ_000059
Alias	Chr01:13724146\|13725373
Organism	Gossypium raimondii
Position	chrChr01: 13724146-13725373 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.001G115700
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.001G115700.2:4 Gorai.001G115700.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATATGCTGCACCAAAATGGGAACTTTTAAAATCGTGCTTTGCACGAGAAAAACTACTGATCACT
AGGCATCGTTTCCTTTACATCTTCAGGACATGCCAGggtgtatgaaggaattaaccaaattaga
aacagaaaataatatacgcccaactccggagattgatgctttcatgaaggtcagttttttttaa
agaagaaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 806
Transcript exons: 13724146-13724222,13724331-13724475,13724716-13725017,13
725092-13725373
GGTGTATGAAGGAATTAACCAAATTAGAAACAGAAAATAATATACGCCCAACTCCGGAGATTGA
TGCTTTCATGAAGGCATCATCTGTCGGGGGTAAAAAGCATAGCATTTCAACAGATTATATCTTA
AAAGTGCTTGGCCTTGACGTATGTTCAGAAACAGTCGTCGGAAGTGACATGCAAAGAGGTGTCT
CCGGTGGACAAAGAAAAAGAGTTACTACAGGAGAAATGATTGTTGGGCCAAGAAAAACTCTTTT
TATGGATGAAATATCCACTGGACTTGATAGTTCTACAACATTCCAAATTGTAAAATGCGTGAAA
AATTTTGTTCATCTAATGGAAGGAACTGTACTCATGGGTCTACTACAACCTGCACCGGAGACAT
ATGAGCTATTTGACAATATAATGTTCATATCTGAGGGATATATGGTGTATCAAGGCCCTCGAGA
AGATGTTTTGGAATTCTTTGAGTCATTAGGATTCAAATTGCCACCGCGTAAGGGTGTTGCAGAT
TTTCTTCAAGAGGTGACCTCTAAAAAAGATCAAGCTCAATACTGGGTTGATCCATCGAAGCCAT
ATGAGTTCATTCCTGTTTCAAAAATGGCGGATGCATTTAAAAATTCTAGATTTGGAAGGTCTCT
AGAGTCAACACTTAATGTTCCATATGATAAATCTCGGATCCATCCTTCAGCTTTGTCCAAAACA
AAATATGCTGCACCAAAATGGGAACTTTTAAAATCGTGCTTTGCACGAGAAAAACTACTGATCA
CTAGGCATCGTTTCCTTTACATCTTCAGGACATGCCAG
						
					

						Genomic sequence
						
							GGTGTATGAAGGAATTAACCAAATTAGAAACAGAAAATAATATACGCCCAACTCCGGAGATTGA
TGCTTTCATGAAGGTCAGTTTTTTTTAAAGAAGAAAAAAGTTATGAGAACTATCGGTGCAATTG
TTCTCATTATTATTATTATTATTTACAAACTTGTTTCTTTTACTTAATTTTTCACAGGCATCAT
CTGTCGGGGGTAAAAAGCATAGCATTTCAACAGATTATATCTTAAAAGTGCTTGGCCTTGACGT
ATGTTCAGAAACAGTCGTCGGAAGTGACATGCAAAGAGGTGTCTCCGGTGGACAAAGAAAAAGA
GTTACTACAGGTGTCTCTTTTGATACAATTTTTTCATCATTGAATTTTGCCAATTAAGATTCTT
ATCGCTTGCATTATCCACATTTGCAGTTATATACAAAGTTACATTTCTATTCTTAAAATCCTTG
GAAGCATGAGATTTTTTTTAATATCTTATAATGCAATATTAATTAATTAAAAAATAAAGACTTT
ACTTAAGGTTATCATTTGACTTTTAGTTTAGATAGATTATGGTATTGTCCCTTTGCAGGAGAAA
TGATTGTTGGGCCAAGAAAAACTCTTTTTATGGATGAAATATCCACTGGACTTGATAGTTCTAC
AACATTCCAAATTGTAAAATGCGTGAAAAATTTTGTTCATCTAATGGAAGGAACTGTACTCATG
GGTCTACTACAACCTGCACCGGAGACATATGAGCTATTTGACAATATAATGTTCATATCTGAGG
GATATATGGTGTATCAAGGCCCTCGAGAAGATGTTTTGGAATTCTTTGAGTCATTAGGATTCAA
ATTGCCACCGCGTAAGGGTGTTGCAGATTTTCTTCAAGAGGTATTCATTTCTCCATAGCTACAT
TTATGGTTAGACATTCTTTAGGTACTTTCCTAACTAGTTGTTGTTGTTAGGTGACCTCTAAAAA
AGATCAAGCTCAATACTGGGTTGATCCATCGAAGCCATATGAGTTCATTCCTGTTTCAAAAATG
GCGGATGCATTTAAAAATTCTAGATTTGGAAGGTCTCTAGAGTCAACACTTAATGTTCCATATG
ATAAATCTCGGATCCATCCTTCAGCTTTGTCCAAAACAAAATATGCTGCACCAAAATGGGAACT
TTTAAAATCGTGCTTTGCACGAGAAAAACTACTGATCACTAGGCATCGTTTCCTTTACATCTTC
AGGACATGCCAG
						
					

			
		

Sequences
Splice junction sequence	ATATGCTGCACCAAAATGGGAACTTTTAAAATCGTGCTTTGCACGAGAAAAACTACTGATCACT AGGCATCGTTTCCTTTACATCTTCAGGACATGCCAGggtgtatgaaggaattaaccaaattaga aacagaaaataatatacgcccaactccggagattgatgctttcatgaaggtcagttttttttaa agaagaaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 806 Transcript exons: 13724146-13724222,13724331-13724475,13724716-13725017,13 725092-13725373 GGTGTATGAAGGAATTAACCAAATTAGAAACAGAAAATAATATACGCCCAACTCCGGAGATTGA TGCTTTCATGAAGGCATCATCTGTCGGGGGTAAAAAGCATAGCATTTCAACAGATTATATCTTA AAAGTGCTTGGCCTTGACGTATGTTCAGAAACAGTCGTCGGAAGTGACATGCAAAGAGGTGTCT CCGGTGGACAAAGAAAAAGAGTTACTACAGGAGAAATGATTGTTGGGCCAAGAAAAACTCTTTT TATGGATGAAATATCCACTGGACTTGATAGTTCTACAACATTCCAAATTGTAAAATGCGTGAAA AATTTTGTTCATCTAATGGAAGGAACTGTACTCATGGGTCTACTACAACCTGCACCGGAGACAT ATGAGCTATTTGACAATATAATGTTCATATCTGAGGGATATATGGTGTATCAAGGCCCTCGAGA AGATGTTTTGGAATTCTTTGAGTCATTAGGATTCAAATTGCCACCGCGTAAGGGTGTTGCAGAT TTTCTTCAAGAGGTGACCTCTAAAAAAGATCAAGCTCAATACTGGGTTGATCCATCGAAGCCAT ATGAGTTCATTCCTGTTTCAAAAATGGCGGATGCATTTAAAAATTCTAGATTTGGAAGGTCTCT AGAGTCAACACTTAATGTTCCATATGATAAATCTCGGATCCATCCTTCAGCTTTGTCCAAAACA AAATATGCTGCACCAAAATGGGAACTTTTAAAATCGTGCTTTGCACGAGAAAAACTACTGATCA CTAGGCATCGTTTCCTTTACATCTTCAGGACATGCCAG
Genomic sequence	GGTGTATGAAGGAATTAACCAAATTAGAAACAGAAAATAATATACGCCCAACTCCGGAGATTGA TGCTTTCATGAAGGTCAGTTTTTTTTAAAGAAGAAAAAAGTTATGAGAACTATCGGTGCAATTG TTCTCATTATTATTATTATTATTTACAAACTTGTTTCTTTTACTTAATTTTTCACAGGCATCAT CTGTCGGGGGTAAAAAGCATAGCATTTCAACAGATTATATCTTAAAAGTGCTTGGCCTTGACGT ATGTTCAGAAACAGTCGTCGGAAGTGACATGCAAAGAGGTGTCTCCGGTGGACAAAGAAAAAGA GTTACTACAGGTGTCTCTTTTGATACAATTTTTTCATCATTGAATTTTGCCAATTAAGATTCTT ATCGCTTGCATTATCCACATTTGCAGTTATATACAAAGTTACATTTCTATTCTTAAAATCCTTG GAAGCATGAGATTTTTTTTAATATCTTATAATGCAATATTAATTAATTAAAAAATAAAGACTTT ACTTAAGGTTATCATTTGACTTTTAGTTTAGATAGATTATGGTATTGTCCCTTTGCAGGAGAAA TGATTGTTGGGCCAAGAAAAACTCTTTTTATGGATGAAATATCCACTGGACTTGATAGTTCTAC AACATTCCAAATTGTAAAATGCGTGAAAAATTTTGTTCATCTAATGGAAGGAACTGTACTCATG GGTCTACTACAACCTGCACCGGAGACATATGAGCTATTTGACAATATAATGTTCATATCTGAGG GATATATGGTGTATCAAGGCCCTCGAGAAGATGTTTTGGAATTCTTTGAGTCATTAGGATTCAA ATTGCCACCGCGTAAGGGTGTTGCAGATTTTCTTCAAGAGGTATTCATTTCTCCATAGCTACAT TTATGGTTAGACATTCTTTAGGTACTTTCCTAACTAGTTGTTGTTGTTAGGTGACCTCTAAAAA AGATCAAGCTCAATACTGGGTTGATCCATCGAAGCCATATGAGTTCATTCCTGTTTCAAAAATG GCGGATGCATTTAAAAATTCTAGATTTGGAAGGTCTCTAGAGTCAACACTTAATGTTCCATATG ATAAATCTCGGATCCATCCTTCAGCTTTGTCCAAAACAAAATATGCTGCACCAAAATGGGAACT TTTAAAATCGTGCTTTGCACGAGAAAAACTACTGATCACTAGGCATCGTTTCCTTTACATCTTC AGGACATGCCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.625886706

Conservation Information
Conserved circRNAs	NA
PMCS	0.625886706

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13725367-13724151(+)
13724151-13724151(+)
13724363-13725362(-)
						
						Potential amino acid sequence
						
							MPGCMKELTKLETENNIRPTPEIDAFMKASSVGGKKHSISTDYILKVLGLDVCSETVVGSDMQR
GVSGGQRKRVTTGEMIVGPRKTLFMDEISTGLDSSTTFQIVKCVKNFVHLMEGTVLMGLLQPAP
ETYELFDNIMFISEGYMVYQGPREDVLEFFESLGFKLPPRKGVADFLQEVTSKKDQAQYWVDPS
KPYEFIPVSKMADAFKNSRFGRSLESTLNVPYDKSRIHPSALSKTKYAAPKWELLKSCFAREKL
LITRHRFLYIFRTCQGV*(+)
MKELTKLETENNIRPTPEIDAFMKASSVGGKKHSISTDYILKVLGLDVCSETVVGSDMQRGVSG
GQRKRVTTGEMIVGPRKTLFMDEISTGLDSSTTFQIVKCVKNFVHLMEGTVLMGLLQPAPETYE
LFDNIMFISEGYMVYQGPREDVLEFFESLGFKLPPRKGVADFLQEVTSKKDQAQYWVDPSKPYE
FIPVSKMADAFKNSRFGRSLESTLNVPYDKSRIHPSALSKTKYAAPKWELLKSCFAREKLLITR
HRFLYIFRTCQGV*(+)
MLCFLPPTDDAFMKASISGVGRILFSVSNLVNSFIHPGMS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13725367-13724151(+) 13724151-13724151(+) 13724363-13725362(-)
Potential amino acid sequence	MPGCMKELTKLETENNIRPTPEIDAFMKASSVGGKKHSISTDYILKVLGLDVCSETVVGSDMQR GVSGGQRKRVTTGEMIVGPRKTLFMDEISTGLDSSTTFQIVKCVKNFVHLMEGTVLMGLLQPAP ETYELFDNIMFISEGYMVYQGPREDVLEFFESLGFKLPPRKGVADFLQEVTSKKDQAQYWVDPS KPYEFIPVSKMADAFKNSRFGRSLESTLNVPYDKSRIHPSALSKTKYAAPKWELLKSCFAREKL LITRHRFLYIFRTCQGV(+) MKELTKLETENNIRPTPEIDAFMKASSVGGKKHSISTDYILKVLGLDVCSETVVGSDMQRGVSG GQRKRVTTGEMIVGPRKTLFMDEISTGLDSSTTFQIVKCVKNFVHLMEGTVLMGLLQPAPETYE LFDNIMFISEGYMVYQGPREDVLEFFESLGFKLPPRKGVADFLQEVTSKKDQAQYWVDPSKPYE FIPVSKMADAFKNSRFGRSLESTLNVPYDKSRIHPSALSKTKYAAPKWELLKSCFAREKLLITR HRFLYIFRTCQGV(+) MLCFLPPTDDAFMKASISGVGRILFSVSNLVNSFIHPGMS*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.001G115700_circ_g.1

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