Plant Circular RNA Database

Detail information of Os02g0132300_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os02g0132300_circ_g.3
					
						ID in PlantcircBase
						osa_circ_012992
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 1681448-1682771  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os02g0132300						
					
						Parent gene annotation
						
							Similar to Erythrocyte membrane protein PFEMP3 (Fragment). (Os02
t0132300-01);Similar to Erythrocyte membrane protein PFEMP3 (Fra
gment). (Os02t0132300-02);Similar to Erythrocyte membrane protei
n PFEMP3 (Fragment). (Os02t0132300-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0132300_circ_g.4 Os02g0132300_circ_g.5 Os02g0132300_circ_g.6 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0132300-03:4
Os02t0132300-01:4
Os02t0132300-02:4

General Information
CircRNA Name	Os02g0132300_circ_g.3
ID in PlantcircBase	osa_circ_012992
Alias	NA
Organism	Oryza sativa
Position	chr2: 1681448-1682771 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os02g0132300
Parent gene annotation	Similar to Erythrocyte membrane protein PFEMP3 (Fragment). (Os02 t0132300-01);Similar to Erythrocyte membrane protein PFEMP3 (Fra gment). (Os02t0132300-02);Similar to Erythrocyte membrane protei n PFEMP3 (Fragment). (Os02t0132300-03)
Parent gene strand	+
Alternative splicing	Os02g0132300_circ_g.4 Os02g0132300_circ_g.5 Os02g0132300_circ_g.6
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0132300-03:4 Os02t0132300-01:4 Os02t0132300-02:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTTGCTTTATTGGGTATGCTAGTATACAGATTTTATGGAGATGAACTACGAGAAATGTTTGGTT
ATGAAGAGCATCCATATGCTTTTTATGCAATGGCAAgatttaatttcaacacatgactggtgaa
atgtttgcttgatgatgtccggacatgatttcagtattgttcataatgcagttggcatctcatg
ccactcaa

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 665
Transcript exons: 1681448-1681776,1681910-1681995,1682117-1682263,1682669-
1682771
GATTTAATTTCAACACATGACTGGTGAAATGTTTGCTTGATGATGTCCGGACATGATTTCAGTA
TTGTTCATAATGCAGTTGGCATCTCATGCCACTCAAGCAGATGAAGCAACAGAGCTTGCTTCCA
GCTGTGATTGTGCAGAAGTGCCAGCTTCACAGCAGATAGTTTCTGAATCAAGTACAGCAGGAAG
CTCCACAGAGCATCTAGTTTCATGTGAAATCAAGCCACTGGGTGTTGATGAAGACATTGAAACT
ATTGATGCTAACGAAGAAACCCACCTTGTCATTCAAGACTGTCCACAATGCCGGATTTGCCTTG
ATAATGAAGGTGATGACTTGATTGCACCTTGTCATTGCAAGGGCACACAGAAATACGTGCATCG
ATCCTGTCTTGATAACTGGAGATCAACAAAGGAAGGTTTTGCCTTTTCACATTGCACTGAGTGT
CGGGCTGCCTTCTTACTTCGTGCAAATGTTCCTCCAGATCGATGGTGGTTGAGATTGAAGTTTC
AACTTCTGGTTGTTAGAGATCACACGTTGATTTTTTTTATTGTACAACTGGTTGTTGCTTTATT
GGGTATGCTAGTATACAGATTTTATGGAGATGAACTACGAGAAATGTTTGGTTATGAAGAGCAT
CCATATGCTTTTTATGCAATGGCAA
						
					

						Genomic sequence
						
							GATTTAATTTCAACACATGACTGGTGAAATGTTTGCTTGATGATGTCCGGACATGATTTCAGTA
TTGTTCATAATGCAGTTGGCATCTCATGCCACTCAAGCAGATGAAGCAACAGAGCTTGCTTCCA
GCTGTGATTGTGCAGAAGTGCCAGCTTCACAGCAGATAGTTTCTGAATCAAGTACAGCAGGAAG
CTCCACAGAGCATCTAGTTTCATGTGAAATCAAGCCACTGGGTGTTGATGAAGACATTGAAACT
ATTGATGCTAACGAAGAAACCCACCTTGTCATTCAAGACTGTCCACAATGCCGGATTTGCCTTG
ATAATGAAGGTCCATTCTGTGACTTGAAGTATCTTTCTTATGGAGTGCCATAAAGGCTTTCGAT
TTTTTTTTTGATAATATTCATATTTTTATGTTGAGTCTACATTTGATCTGATATTTTTTCCATA
TCCACTACAAGCAGGTGATGACTTGATTGCACCTTGTCATTGCAAGGGCACACAGAAATACGTG
CATCGATCCTGTCTTGATAACTGGAGATCAACAAAGGTGGATTCATGAACATTTGCTCTTTATG
CTTCATGTACCTATATTTACTGCCATCGCAGAATCCAGTTAATCATTAACCTAATCCAGCTCTG
ACCTAGTTTCTATGTTACATTTCCACCAGGAAGGTTTTGCCTTTTCACATTGCACTGAGTGTCG
GGCTGCCTTCTTACTTCGTGCAAATGTTCCTCCAGATCGATGGTGGTTGAGATTGAAGTTTCAA
CTTCTGGTTGTTAGAGATCACACGTTGATTTTTTTTATTGTACAACTGGTACGAGTTCCACACC
TTCTGTAATCTAATTCAACAAACTGTTTTTTCTTTACTGAACCAATACTGAATACATCTCAGTA
GGATTTCTCCAACTGTTTACCCTAAAAAATGAACATGCTACAATACTGAACTCAAGCATGACAT
GGTTGCACCTTATTCTGTTAATGTATAAAGCAGTAATGATTTTATGGATTTATATTGATATCAC
CATTTCTTTGAAGAGATGTGGTAGTTAGTTCACTGACTTCTTACTAATACATTGCCATGATTTC
TGGCGTGAGATCCAAAAGATAAACGTTTTTGAATAAATGCACAAGAAAGAGGCCTGAAAATGGT
CACGATGTGCTGCTGTAAGATGCATCTTTGTTTTTGTCTTACAATCTTATCCGTTTTCCTGCCT
GATAGGTTGTTGCTTTATTGGGTATGCTAGTATACAGATTTTATGGAGATGAACTACGAGAAAT
GTTTGGTTATGAAGAGCATCCATATGCTTTTTATGCAATGGCAA
						
					

			
		

Sequences
Splice junction sequence	GTTGCTTTATTGGGTATGCTAGTATACAGATTTTATGGAGATGAACTACGAGAAATGTTTGGTT ATGAAGAGCATCCATATGCTTTTTATGCAATGGCAAgatttaatttcaacacatgactggtgaa atgtttgcttgatgatgtccggacatgatttcagtattgttcataatgcagttggcatctcatg ccactcaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 665 Transcript exons: 1681448-1681776,1681910-1681995,1682117-1682263,1682669- 1682771 GATTTAATTTCAACACATGACTGGTGAAATGTTTGCTTGATGATGTCCGGACATGATTTCAGTA TTGTTCATAATGCAGTTGGCATCTCATGCCACTCAAGCAGATGAAGCAACAGAGCTTGCTTCCA GCTGTGATTGTGCAGAAGTGCCAGCTTCACAGCAGATAGTTTCTGAATCAAGTACAGCAGGAAG CTCCACAGAGCATCTAGTTTCATGTGAAATCAAGCCACTGGGTGTTGATGAAGACATTGAAACT ATTGATGCTAACGAAGAAACCCACCTTGTCATTCAAGACTGTCCACAATGCCGGATTTGCCTTG ATAATGAAGGTGATGACTTGATTGCACCTTGTCATTGCAAGGGCACACAGAAATACGTGCATCG ATCCTGTCTTGATAACTGGAGATCAACAAAGGAAGGTTTTGCCTTTTCACATTGCACTGAGTGT CGGGCTGCCTTCTTACTTCGTGCAAATGTTCCTCCAGATCGATGGTGGTTGAGATTGAAGTTTC AACTTCTGGTTGTTAGAGATCACACGTTGATTTTTTTTATTGTACAACTGGTTGTTGCTTTATT GGGTATGCTAGTATACAGATTTTATGGAGATGAACTACGAGAAATGTTTGGTTATGAAGAGCAT CCATATGCTTTTTATGCAATGGCAA
Genomic sequence	GATTTAATTTCAACACATGACTGGTGAAATGTTTGCTTGATGATGTCCGGACATGATTTCAGTA TTGTTCATAATGCAGTTGGCATCTCATGCCACTCAAGCAGATGAAGCAACAGAGCTTGCTTCCA GCTGTGATTGTGCAGAAGTGCCAGCTTCACAGCAGATAGTTTCTGAATCAAGTACAGCAGGAAG CTCCACAGAGCATCTAGTTTCATGTGAAATCAAGCCACTGGGTGTTGATGAAGACATTGAAACT ATTGATGCTAACGAAGAAACCCACCTTGTCATTCAAGACTGTCCACAATGCCGGATTTGCCTTG ATAATGAAGGTCCATTCTGTGACTTGAAGTATCTTTCTTATGGAGTGCCATAAAGGCTTTCGAT TTTTTTTTTGATAATATTCATATTTTTATGTTGAGTCTACATTTGATCTGATATTTTTTCCATA TCCACTACAAGCAGGTGATGACTTGATTGCACCTTGTCATTGCAAGGGCACACAGAAATACGTG CATCGATCCTGTCTTGATAACTGGAGATCAACAAAGGTGGATTCATGAACATTTGCTCTTTATG CTTCATGTACCTATATTTACTGCCATCGCAGAATCCAGTTAATCATTAACCTAATCCAGCTCTG ACCTAGTTTCTATGTTACATTTCCACCAGGAAGGTTTTGCCTTTTCACATTGCACTGAGTGTCG GGCTGCCTTCTTACTTCGTGCAAATGTTCCTCCAGATCGATGGTGGTTGAGATTGAAGTTTCAA CTTCTGGTTGTTAGAGATCACACGTTGATTTTTTTTATTGTACAACTGGTACGAGTTCCACACC TTCTGTAATCTAATTCAACAAACTGTTTTTTCTTTACTGAACCAATACTGAATACATCTCAGTA GGATTTCTCCAACTGTTTACCCTAAAAAATGAACATGCTACAATACTGAACTCAAGCATGACAT GGTTGCACCTTATTCTGTTAATGTATAAAGCAGTAATGATTTTATGGATTTATATTGATATCAC CATTTCTTTGAAGAGATGTGGTAGTTAGTTCACTGACTTCTTACTAATACATTGCCATGATTTC TGGCGTGAGATCCAAAAGATAAACGTTTTTGAATAAATGCACAAGAAAGAGGCCTGAAAATGGT CACGATGTGCTGCTGTAAGATGCATCTTTGTTTTTGTCTTACAATCTTATCCGTTTTCCTGCCT GATAGGTTGTTGCTTTATTGGGTATGCTAGTATACAGATTTTATGGAGATGAACTACGAGAAAT GTTTGGTTATGAAGAGCATCCATATGCTTTTTATGCAATGGCAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_011745 						
					
						PMCS
						0.271035719

Conservation Information
Conserved circRNAs	osi_circ_011745
PMCS	0.271035719

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							1681501-1681464(+)
1682707-1681471(+)
						
						Potential amino acid sequence
						
							MISVLFIMQLASHATQADEATELASSCDCAEVPASQQIVSESSTAGSSTEHLVSCEIKPLGVDE
DIETIDANEETHLVIQDCPQCRICLDNEGDDLIAPCHCKGTQKYVHRSCLDNWRSTKEGFAFSH
CTECRAAFLLRANVPPDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVYRFYGDELREMFG
YEEHPYAFYAMARFNFNT*(+)
MEMNYEKCLVMKSIHMLFMQWQDLISTHDW*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	1681501-1681464(+) 1682707-1681471(+)
Potential amino acid sequence	MISVLFIMQLASHATQADEATELASSCDCAEVPASQQIVSESSTAGSSTEHLVSCEIKPLGVDE DIETIDANEETHLVIQDCPQCRICLDNEGDDLIAPCHCKGTQKYVHRSCLDNWRSTKEGFAFSH CTECRAAFLLRANVPPDRWWLRLKFQLLVVRDHTLIFFIVQLVVALLGMLVYRFYGDELREMFG YEEHPYAFYAMARFNFNT(+) MEMNYEKCLVMKSIHMLFMQWQDLISTHDW(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os02g0132300_circ_g.3

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