Plant Circular RNA Database

Detail information of orai.008G215500_circ_g.2

			
						 General Information
					
						CircRNA Name
						orai.008G215500_circ_g.2
					
						ID in PlantcircBase
						gra_circ_000868
					
						Alias
						Chr08:50259744|50260913
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr08: 50259744-50260913  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.008G215500						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						13/48
					
						Tissues
						leaf/ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.008G215500.3:4
Gorai.008G215500.1:4
Gorai.008G215500.2:4

General Information
CircRNA Name	orai.008G215500_circ_g.2
ID in PlantcircBase	gra_circ_000868
Alias	Chr08:50259744\|50260913
Organism	Gossypium raimondii
Position	chrChr08: 50259744-50260913 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.008G215500
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	13/48
Tissues	leaf/ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.008G215500.3:4 Gorai.008G215500.1:4 Gorai.008G215500.2:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCCAGGATGCTGTTAGCCGAAAAATCAGCCTGGACACGTTAGTCGAGTTTGCATTGGCTCAAGG
ATCCCAATGGATATTTATCACACCTCATGATATCAGatttaatggccatttgggaaagaaaggt
atcagtggacaaattaatgttagttatgaagaaaagaccctttcagtagaggtaagtatatcaa
acttctat

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 330
Transcript exons: 50259744-50259822,50259961-50260018,50260245-50260342,50
260819-50260913
ATTTAATGGCCATTTGGGAAAGAAAGGTATCAGTGGACAAATTAATGTTAGTTATGAAGAAAAG
ACCCTTTCAGTAGAGGTTAAAATGCCTCAAGATGCATCAAGCACCATCGTTCGTGATACAAGAG
GGCTCTCAGGAGGAGAGCGGTCCTTCTCAACATTATGTTTTGCGTTGGCTCTTCATGATATGAC
TGAAGCCCCATTTCGAGCTATGGATGAGTTTGATGTGTTCATGGATGCTGTTAGCCGAAAAATC
AGCCTGGACACGTTAGTCGAGTTTGCATTGGCTCAAGGATCCCAATGGATATTTATCACACCTC
ATGATATCAG
						
					

						Genomic sequence
						
							ATTTAATGGCCATTTGGGAAAGAAAGGTATCAGTGGACAAATTAATGTTAGTTATGAAGAAAAG
ACCCTTTCAGTAGAGGTAAGTATATCAAACTTCTATTTTTCTTTTAGGTGTAATTTCAATAGAC
TACTTACGTCTTTCACCAGGTCACCGTGCCAGTTTAAAGTTCTCTGCATTTTATTATGGAATTT
TCTGATCCTGTTATTGGTCTAACAGGTTAAAATGCCTCAAGATGCATCAAGCACCATCGTTCGT
GATACAAGAGGGCTCTCAGGTTTTATATTTCCGTTCCATATACCTTTTAGAATGTGTTCATAAG
AGTTTCAAGTTCACAACACAATATAGATTTATCTGCTTGTGGCCTCATTAATAGTGTTTAATCC
GGCATAAATGTATCTATGTGGAAAATTTTTACTCCCATGTCATCCAAATTAGCAGTCAGACATT
TTCATAATTGATTAATGTTAACTCTAGTGTTATTTTGATAATTTGTTGATTAGGAGGAGAGCGG
TCCTTCTCAACATTATGTTTTGCGTTGGCTCTTCATGATATGACTGAAGCCCCATTTCGAGCTA
TGGATGAGTTTGATGTGTTCATGGTGAGATTTGGGTTTTCTTTGGGGTGTGTGTGTTATCCCTT
GGGGATTGAGGTATATGTGATCGTCCATCAAGAAGCATAAAACTATAGACTGAGCAGATTCATA
TGTAGCGGTGTAATCGAGCTGAGCCTGAATACTGCCAAGCTCGAGCTTGGTCAAACATCTTTTC
TTAAGCTCGTTTGCCGATGTTTGTACTCGAGTTCATCTCAAGAATTAAACTTAGGATTAATAAT
ATATATTAAAAGAAATAACGTAATTTAATAATATAAAAATATGAAAAGCTTGATAAGGCTTAGA
AGCCGTTGGAGTCAAAGTATTACTAAGTTCGAATTCGGTTTGACCGATAGATTGAGTTGCTCGA
GCTCGGCTCTATAATTACTGTATCAAGTTTGAATTTTTTTTCGAGTCGAACTGTAATAGTGTGC
GAGTACTAGTGTCTCATTCACACTCCTATTCATATGTATATTTTCCTCCAGGATGCTGTTAGCC
GAAAAATCAGCCTGGACACGTTAGTCGAGTTTGCATTGGCTCAAGGATCCCAATGGATATTTAT
CACACCTCATGATATCAG
						
					

			
		

Sequences
Splice junction sequence	TCCAGGATGCTGTTAGCCGAAAAATCAGCCTGGACACGTTAGTCGAGTTTGCATTGGCTCAAGG ATCCCAATGGATATTTATCACACCTCATGATATCAGatttaatggccatttgggaaagaaaggt atcagtggacaaattaatgttagttatgaagaaaagaccctttcagtagaggtaagtatatcaa acttctat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 330 Transcript exons: 50259744-50259822,50259961-50260018,50260245-50260342,50 260819-50260913 ATTTAATGGCCATTTGGGAAAGAAAGGTATCAGTGGACAAATTAATGTTAGTTATGAAGAAAAG ACCCTTTCAGTAGAGGTTAAAATGCCTCAAGATGCATCAAGCACCATCGTTCGTGATACAAGAG GGCTCTCAGGAGGAGAGCGGTCCTTCTCAACATTATGTTTTGCGTTGGCTCTTCATGATATGAC TGAAGCCCCATTTCGAGCTATGGATGAGTTTGATGTGTTCATGGATGCTGTTAGCCGAAAAATC AGCCTGGACACGTTAGTCGAGTTTGCATTGGCTCAAGGATCCCAATGGATATTTATCACACCTC ATGATATCAG
Genomic sequence	ATTTAATGGCCATTTGGGAAAGAAAGGTATCAGTGGACAAATTAATGTTAGTTATGAAGAAAAG ACCCTTTCAGTAGAGGTAAGTATATCAAACTTCTATTTTTCTTTTAGGTGTAATTTCAATAGAC TACTTACGTCTTTCACCAGGTCACCGTGCCAGTTTAAAGTTCTCTGCATTTTATTATGGAATTT TCTGATCCTGTTATTGGTCTAACAGGTTAAAATGCCTCAAGATGCATCAAGCACCATCGTTCGT GATACAAGAGGGCTCTCAGGTTTTATATTTCCGTTCCATATACCTTTTAGAATGTGTTCATAAG AGTTTCAAGTTCACAACACAATATAGATTTATCTGCTTGTGGCCTCATTAATAGTGTTTAATCC GGCATAAATGTATCTATGTGGAAAATTTTTACTCCCATGTCATCCAAATTAGCAGTCAGACATT TTCATAATTGATTAATGTTAACTCTAGTGTTATTTTGATAATTTGTTGATTAGGAGGAGAGCGG TCCTTCTCAACATTATGTTTTGCGTTGGCTCTTCATGATATGACTGAAGCCCCATTTCGAGCTA TGGATGAGTTTGATGTGTTCATGGTGAGATTTGGGTTTTCTTTGGGGTGTGTGTGTTATCCCTT GGGGATTGAGGTATATGTGATCGTCCATCAAGAAGCATAAAACTATAGACTGAGCAGATTCATA TGTAGCGGTGTAATCGAGCTGAGCCTGAATACTGCCAAGCTCGAGCTTGGTCAAACATCTTTTC TTAAGCTCGTTTGCCGATGTTTGTACTCGAGTTCATCTCAAGAATTAAACTTAGGATTAATAAT ATATATTAAAAGAAATAACGTAATTTAATAATATAAAAATATGAAAAGCTTGATAAGGCTTAGA AGCCGTTGGAGTCAAAGTATTACTAAGTTCGAATTCGGTTTGACCGATAGATTGAGTTGCTCGA GCTCGGCTCTATAATTACTGTATCAAGTTTGAATTTTTTTTCGAGTCGAACTGTAATAGTGTGC GAGTACTAGTGTCTCATTCACACTCCTATTCATATGTATATTTTCCTCCAGGATGCTGTTAGCC GAAAAATCAGCCTGGACACGTTAGTCGAGTTTGCATTGGCTCAAGGATCCCAATGGATATTTAT CACACCTCATGATATCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							gar_circ_000713 						
					
						PMCS
						0.869650719

Conservation Information
Conserved circRNAs	gar_circ_000713
PMCS	0.869650719

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							50259967-50260910(+)
50260904-50259817(+)
50259757-50260854(-)
						
						Potential amino acid sequence
						
							MPQDASSTIVRDTRGLSGGERSFSTLCFALALHDMTEAPFRAMDEFDVFMDAVSRKISLDTLVE
FALAQGSQWIFITPHDIRFNGHLGKKGISGQINVSYEEKTLSVEVKMPQDASSTIVRDTRGLSG
GERSFSTLCFALALHDMTEAPFRAMDEFDVFMDAVSRKISLDTLVEFALAQGSQWIFITPHDIR
FNGHLGKKGISGQINVSYEEKTLSVEVKMPQDASSTIVRDTRGLSGGERSFSTLCFALALHDMT
EAPFRAMDEFDVFMDAVSRKISLDTLVEFALAQGSQWIFITPHDIRFNGHLGKKGISGQINVSY
EEKTLSVEVKMPQDASSTIVRDTRGLSGGERSFSTLCFALALHDMTEAPFRAMDEFDVFMDAVS
RKISLDTLVEFALAQGSQWIFITPHDI(+)
MISDLMAIWERKVSVDKLMLVMKKRPFQ*(+)
MAIKSDIMRCDKYPLGSLSQCKLD*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	50259967-50260910(+) 50260904-50259817(+) 50259757-50260854(-)
Potential amino acid sequence	MPQDASSTIVRDTRGLSGGERSFSTLCFALALHDMTEAPFRAMDEFDVFMDAVSRKISLDTLVE FALAQGSQWIFITPHDIRFNGHLGKKGISGQINVSYEEKTLSVEVKMPQDASSTIVRDTRGLSG GERSFSTLCFALALHDMTEAPFRAMDEFDVFMDAVSRKISLDTLVEFALAQGSQWIFITPHDIR FNGHLGKKGISGQINVSYEEKTLSVEVKMPQDASSTIVRDTRGLSGGERSFSTLCFALALHDMT EAPFRAMDEFDVFMDAVSRKISLDTLVEFALAQGSQWIFITPHDIRFNGHLGKKGISGQINVSY EEKTLSVEVKMPQDASSTIVRDTRGLSGGERSFSTLCFALALHDMTEAPFRAMDEFDVFMDAVS RKISLDTLVEFALAQGSQWIFITPHDI(+) MISDLMAIWERKVSVDKLMLVMKKRPFQ(+) MAIKSDIMRCDKYPLGSLSQCKLD(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.008G215500_circ_g.2

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