Plant Circular RNA Database

Detail information of Os06g0329200_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os06g0329200_circ_g.2
					
						ID in PlantcircBase
						osa_circ_030717
					
						Alias
						Os_ciR10335
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 12992822-12994002  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Os06g0329200						
					
						Parent gene annotation
						
							Conserved hypothetical protein. (Os06t0329200-01);Hypothetical g
ene. (Os06t0329200-02)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os06g0329200_circ_g.1 Os06g0329200_circ_g.3 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0329200-01:3

General Information
CircRNA Name	Os06g0329200_circ_g.2
ID in PlantcircBase	osa_circ_030717
Alias	Os_ciR10335
Organism	Oryza sativa
Position	chr6: 12992822-12994002 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	find_circ
Parent gene	Os06g0329200
Parent gene annotation	Conserved hypothetical protein. (Os06t0329200-01);Hypothetical g ene. (Os06t0329200-02)
Parent gene strand	-
Alternative splicing	Os06g0329200_circ_g.1 Os06g0329200_circ_g.3
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0329200-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GATATCGTGGAAATCCCATAGCCTAGAGAGCCGTGTCTTGATCTGCTTGCTGATGTCTCCATAC
AGAAGCTGAGAAATAAGGATATACGCTATAGTGAGCctctgtttcagggcagcaacttcgggca
tatcaaggttgacataccatttgcacggtgagcttccagtcaaagtcaggcctgcattggatta
gaccagtg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 960
Transcript exons: 12992822-12992900,12992981-12993710,12993852-12994002
CTCTGTTTCAGGGCAGCAACTTCGGGCATATCAAGGTTGACATACCATTTGCACGGTGAGCTTC
CAGTCAAAGTCAGGCCTATCCCTTTGTAGTTTCGTACAAGTGTTCCAACAAAGACAACAACTTG
AGGCTCAATCTGTCCAACATTGAATATCGCCTGTACTTCAAAGGCAGTTGTTGTTTTCCCCCAG
AGTGTGACAGGCAGTGTGCAGCCGCTGTTTTTTGTAAATTGTGCATGGTTTATTTGGTGTTGGA
TATTAGTAGCTTGTTAGGGTGGTTAGTGCACGTGTCGTGAGCCAAACATAGTGCATAATGAAAG
AAGAAAAATAGGTGCTATTACTTACCTTGCATCTGAAATTTGCATGGTTCTTTTCAGGTTGTCG
GTGTTGCTTGATTTAGGGCGGATGGTGGCTGTTGCACCAACCTCAGTTATGATACCCATAACAT
CTGCCCACATTGAAAATTAGTTTGATAAATATAGGGCCGCAACTGGAAACAAACATAGATGACA
AATGTGATCGAAAGGAGACGCACCAACATAGAAATGATTCTTTTCCACAAGAGATGGAACATCT
TGGAACGGTGTCAGCGAAAACGTGATGCTAGGGAAATTGTCAGGGATCTCAATGCATTCTTCCA
AGGTAGTCCATTTAGTAAATGAAATCATCAAAGGATTCGCAACAGGCCTATATGTTCTATTGGC
ATATCTGACAGAGAAAGAGTCGATGTAGTAGACCTTCCCTTGCTGTAAGAGATCCTTGAACTTA
TTTCTTAGGGATGGATAGATTTGCACATGTATACTGGCGCCCGTTTCATCCAGCAAGACAAGTT
CAGTGTGATATATTTTTTCTTCATCATTGATATCGTGGAAATCCCATAGCCTAGAGAGCCGTGT
CTTGATCTGCTTGCTGATGTCTCCATACAGAAGCTGAGAAATAAGGATATACGCTATAGTGAGC
						
					

						Genomic sequence
						
							CTCTGTTTCAGGGCAGCAACTTCGGGCATATCAAGGTTGACATACCATTTGCACGGTGAGCTTC
CAGTCAAAGTCAGGCCTGCATTGGATTAGACCAGTGTTAGATAGAAGCTCATACAATGTGTGCA
GGAATAATTTGATAGGCTGGTACATGTATACCTATCCCTTTGTAGTTTCGTACAAGTGTTCCAA
CAAAGACAACAACTTGAGGCTCAATCTGTCCAACATTGAATATCGCCTGTACTTCAAAGGCAGT
TGTTGTTTTCCCCCAGAGTGTGACAGGCAGTGTGCAGCCGCTGTTTTTTGTAAATTGTGCATGG
TTTATTTGGTGTTGGATATTAGTAGCTTGTTAGGGTGGTTAGTGCACGTGTCGTGAGCCAAACA
TAGTGCATAATGAAAGAAGAAAAATAGGTGCTATTACTTACCTTGCATCTGAAATTTGCATGGT
TCTTTTCAGGTTGTCGGTGTTGCTTGATTTAGGGCGGATGGTGGCTGTTGCACCAACCTCAGTT
ATGATACCCATAACATCTGCCCACATTGAAAATTAGTTTGATAAATATAGGGCCGCAACTGGAA
ACAAACATAGATGACAAATGTGATCGAAAGGAGACGCACCAACATAGAAATGATTCTTTTCCAC
AAGAGATGGAACATCTTGGAACGGTGTCAGCGAAAACGTGATGCTAGGGAAATTGTCAGGGATC
TCAATGCATTCTTCCAAGGTAGTCCATTTAGTAAATGAAATCATCAAAGGATTCGCAACAGGCC
TATATGTTCTATTGGCATATCTGACAGAGAAAGAGTCGATGTAGTAGACCTTCCCTTGCTGTAA
GAGATCCTTGAACTTATTTCTTAGGGATGGATAGATTTGCACATGTATACTGGCGCCCTACAAT
ATACAGCGGCATGTCAGTGCTATTTAAAGTATGCACTGGAGAATGAATGGAGTGTGTGTGGGAG
ACGAGGAAGAGGGAGAATGTGTGTGTGGGAGACGAGGAAGAGGGAGAATGGTTGTGCGGTCAAT
GATTACCGTTTCATCCAGCAAGACAAGTTCAGTGTGATATATTTTTTCTTCATCATTGATATCG
TGGAAATCCCATAGCCTAGAGAGCCGTGTCTTGATCTGCTTGCTGATGTCTCCATACAGAAGCT
GAGAAATAAGGATATACGCTATAGTGAGC
						
					

			
		

Sequences
Splice junction sequence	GATATCGTGGAAATCCCATAGCCTAGAGAGCCGTGTCTTGATCTGCTTGCTGATGTCTCCATAC AGAAGCTGAGAAATAAGGATATACGCTATAGTGAGCctctgtttcagggcagcaacttcgggca tatcaaggttgacataccatttgcacggtgagcttccagtcaaagtcaggcctgcattggatta gaccagtg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 960 Transcript exons: 12992822-12992900,12992981-12993710,12993852-12994002 CTCTGTTTCAGGGCAGCAACTTCGGGCATATCAAGGTTGACATACCATTTGCACGGTGAGCTTC CAGTCAAAGTCAGGCCTATCCCTTTGTAGTTTCGTACAAGTGTTCCAACAAAGACAACAACTTG AGGCTCAATCTGTCCAACATTGAATATCGCCTGTACTTCAAAGGCAGTTGTTGTTTTCCCCCAG AGTGTGACAGGCAGTGTGCAGCCGCTGTTTTTTGTAAATTGTGCATGGTTTATTTGGTGTTGGA TATTAGTAGCTTGTTAGGGTGGTTAGTGCACGTGTCGTGAGCCAAACATAGTGCATAATGAAAG AAGAAAAATAGGTGCTATTACTTACCTTGCATCTGAAATTTGCATGGTTCTTTTCAGGTTGTCG GTGTTGCTTGATTTAGGGCGGATGGTGGCTGTTGCACCAACCTCAGTTATGATACCCATAACAT CTGCCCACATTGAAAATTAGTTTGATAAATATAGGGCCGCAACTGGAAACAAACATAGATGACA AATGTGATCGAAAGGAGACGCACCAACATAGAAATGATTCTTTTCCACAAGAGATGGAACATCT TGGAACGGTGTCAGCGAAAACGTGATGCTAGGGAAATTGTCAGGGATCTCAATGCATTCTTCCA AGGTAGTCCATTTAGTAAATGAAATCATCAAAGGATTCGCAACAGGCCTATATGTTCTATTGGC ATATCTGACAGAGAAAGAGTCGATGTAGTAGACCTTCCCTTGCTGTAAGAGATCCTTGAACTTA TTTCTTAGGGATGGATAGATTTGCACATGTATACTGGCGCCCGTTTCATCCAGCAAGACAAGTT CAGTGTGATATATTTTTTCTTCATCATTGATATCGTGGAAATCCCATAGCCTAGAGAGCCGTGT CTTGATCTGCTTGCTGATGTCTCCATACAGAAGCTGAGAAATAAGGATATACGCTATAGTGAGC
Genomic sequence	CTCTGTTTCAGGGCAGCAACTTCGGGCATATCAAGGTTGACATACCATTTGCACGGTGAGCTTC CAGTCAAAGTCAGGCCTGCATTGGATTAGACCAGTGTTAGATAGAAGCTCATACAATGTGTGCA GGAATAATTTGATAGGCTGGTACATGTATACCTATCCCTTTGTAGTTTCGTACAAGTGTTCCAA CAAAGACAACAACTTGAGGCTCAATCTGTCCAACATTGAATATCGCCTGTACTTCAAAGGCAGT TGTTGTTTTCCCCCAGAGTGTGACAGGCAGTGTGCAGCCGCTGTTTTTTGTAAATTGTGCATGG TTTATTTGGTGTTGGATATTAGTAGCTTGTTAGGGTGGTTAGTGCACGTGTCGTGAGCCAAACA TAGTGCATAATGAAAGAAGAAAAATAGGTGCTATTACTTACCTTGCATCTGAAATTTGCATGGT TCTTTTCAGGTTGTCGGTGTTGCTTGATTTAGGGCGGATGGTGGCTGTTGCACCAACCTCAGTT ATGATACCCATAACATCTGCCCACATTGAAAATTAGTTTGATAAATATAGGGCCGCAACTGGAA ACAAACATAGATGACAAATGTGATCGAAAGGAGACGCACCAACATAGAAATGATTCTTTTCCAC AAGAGATGGAACATCTTGGAACGGTGTCAGCGAAAACGTGATGCTAGGGAAATTGTCAGGGATC TCAATGCATTCTTCCAAGGTAGTCCATTTAGTAAATGAAATCATCAAAGGATTCGCAACAGGCC TATATGTTCTATTGGCATATCTGACAGAGAAAGAGTCGATGTAGTAGACCTTCCCTTGCTGTAA GAGATCCTTGAACTTATTTCTTAGGGATGGATAGATTTGCACATGTATACTGGCGCCCTACAAT ATACAGCGGCATGTCAGTGCTATTTAAAGTATGCACTGGAGAATGAATGGAGTGTGTGTGGGAG ACGAGGAAGAGGGAGAATGTGTGTGTGGGAGACGAGGAAGAGGGAGAATGGTTGTGCGGTCAAT GATTACCGTTTCATCCAGCAAGACAAGTTCAGTGTGATATATTTTTTCTTCATCATTGATATCG TGGAAATCCCATAGCCTAGAGAGCCGTGTCTTGATCTGCTTGCTGATGTCTCCATACAGAAGCT GAGAAATAAGGATATACGCTATAGTGAGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_016137 						
					
						PMCS
						0.410206298

Conservation Information
Conserved circRNAs	osi_circ_016137
PMCS	0.410206298

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12992852-12993368(-)
						
						Potential amino acid sequence
						
							MPEVAALKQRLTIAYILISQLLYGDISKQIKTRLSRLWDFHDINDEEKIYHTELVLLDETGASI
HVQIYPSLRNKFKDLLQQGKVYYIDSFSVRYANRTYRPVANPLMISFTKWTTLEECIEIPDNFP
SITFSLTPFQDVPSLVEKNHFYVGASPFDHICHLCLFPVAALYLSN*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12992852-12993368(-)
Potential amino acid sequence	MPEVAALKQRLTIAYILISQLLYGDISKQIKTRLSRLWDFHDINDEEKIYHTELVLLDETGASI HVQIYPSLRNKFKDLLQQGKVYYIDSFSVRYANRTYRPVANPLMISFTKWTTLEECIEIPDNFP SITFSLTPFQDVPSLVEKNHFYVGASPFDHICHLCLFPVAALYLSN*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of Os06g0329200_circ_g.2

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