Plant Circular RNA Database

Detail information of Os08g0100500_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os08g0100500_circ_g.2
					
						ID in PlantcircBase
						osa_circ_035511
					
						Alias
						Os_ciR2062
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr8: 40597-41153  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, find_circ
					
						Parent gene
						
							Os08g0100500						
					
						Parent gene annotation
						
							Regulation of nuclear pre-mRNA protein domain containing protein
. (Os08t0100500-01);Conserved hypothetical protein. (Os08t010050
0-02);Hypothetical conserved gene. (Os08t0100500-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os08g0100500_circ_g.1 						
					
						Support reads
						11/4
					
						Tissues
						root/shoot, root, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os08t0100500-01:3

General Information
CircRNA Name	Os08g0100500_circ_g.2
ID in PlantcircBase	osa_circ_035511
Alias	Os_ciR2062
Organism	Oryza sativa
Position	chr8: 40597-41153 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, find_circ
Parent gene	Os08g0100500
Parent gene annotation	Regulation of nuclear pre-mRNA protein domain containing protein . (Os08t0100500-01);Conserved hypothetical protein. (Os08t010050 0-02);Hypothetical conserved gene. (Os08t0100500-03)
Parent gene strand	+
Alternative splicing	Os08g0100500_circ_g.1
Support reads	11/4
Tissues	root/shoot, root, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os08t0100500-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTAACACAGGTTTTCTGCTGAGGCGACCATCTCGAGCTGAACGCTCTGTAGATGATCCTATTAG
GGAGATGGAAGGCATGCTTGTTGATGAGTATGGAAGattgtggagcttcttgtgctgaaattag
agaatgagcctaatttgcatcgaagagttgacctcctttttcttatagactccataactcagtg
ttctcact

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 103
Transcript exons: 40597-40671,41126-41153
ATTGTGGAGCTTCTTGTGCTGAAATTAGAGAATGAGCCTAATTTGCATCGAAGAGTTGACCTCC
TTTTTCTTATAAAGGCATGCTTGTTGATGAGTATGGAAG
Transcript length: 410
Transcript exons: 40597-40705,40783-40895,40966-41153
ATTGTGGAGCTTCTTGTGCTGAAATTAGAGAATGAGCCTAATTTGCATCGAAGAGTTGACCTCC
TTTTTCTTATAGACTCCATAACTCAGTGTTCTCACTCTCAGAAAGGTGTTGCTGGCGTTTCATA
TGTTCCCACCGTTCAAGCTGCGCTACCTCGTCTCTTGGGCGCTGCTGCTCCACCAGGACCCGGT
GCTCGTGAAAATCGACGACAGTGCCTCAAAGTTTTACGGTTATGGCTTGAGAGAAAGATTATGC
CAGAAGGCATACTTCGAAGATACATGGATGATATTGAGGTGCCAAATGATGATGCTAACACAGG
TTTTCTGCTGAGGCGACCATCTCGAGCTGAACGCTCTGTAGATGATCCTATTAGGGAGATGGAA
GGCATGCTTGTTGATGAGTATGGAAG
						
						Genomic sequence
						
							ATTGTGGAGCTTCTTGTGCTGAAATTAGAGAATGAGCCTAATTTGCATCGAAGAGTTGACCTCC
TTTTTCTTATAGACTCCATAACTCAGTGTTCTCACTCTCAGAAAGGTAATGTGCTATCCTCAGA
ATTATTTGTTCTTAACAAGCTAATGTGTAGTAATTTATCTGAATTCTGAAACGTTTAGGTGTTG
CTGGCGTTTCATATGTTCCCACCGTTCAAGCTGCGCTACCTCGTCTCTTGGGCGCTGCTGCTCC
ACCAGGACCCGGTGCTCGTGAAAATCGACGACAGTGCCTCAAAGTAATGACTTAATTACATGTT
CTTATTTTACTTCCTAATGGTCAATCATTTCTATCTTCTGGCTTTATAGGTTTTACGGTTATGG
CTTGAGAGAAAGATTATGCCAGAAGGCATACTTCGAAGATACATGGATGATATTGAGGTGCCAA
ATGATGATGCTAACACAGGTTTTCTGCTGAGGCGACCATCTCGAGCTGAACGCTCTGTAGATGA
TCCTATTAGGGAGATGGAAGGCATGCTTGTTGATGAGTATGGAAG

Sequences
Splice junction sequence	CTAACACAGGTTTTCTGCTGAGGCGACCATCTCGAGCTGAACGCTCTGTAGATGATCCTATTAG GGAGATGGAAGGCATGCTTGTTGATGAGTATGGAAGattgtggagcttcttgtgctgaaattag agaatgagcctaatttgcatcgaagagttgacctcctttttcttatagactccataactcagtg ttctcact
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 103 Transcript exons: 40597-40671,41126-41153 ATTGTGGAGCTTCTTGTGCTGAAATTAGAGAATGAGCCTAATTTGCATCGAAGAGTTGACCTCC TTTTTCTTATAAAGGCATGCTTGTTGATGAGTATGGAAG Transcript length: 410 Transcript exons: 40597-40705,40783-40895,40966-41153 ATTGTGGAGCTTCTTGTGCTGAAATTAGAGAATGAGCCTAATTTGCATCGAAGAGTTGACCTCC TTTTTCTTATAGACTCCATAACTCAGTGTTCTCACTCTCAGAAAGGTGTTGCTGGCGTTTCATA TGTTCCCACCGTTCAAGCTGCGCTACCTCGTCTCTTGGGCGCTGCTGCTCCACCAGGACCCGGT GCTCGTGAAAATCGACGACAGTGCCTCAAAGTTTTACGGTTATGGCTTGAGAGAAAGATTATGC CAGAAGGCATACTTCGAAGATACATGGATGATATTGAGGTGCCAAATGATGATGCTAACACAGG TTTTCTGCTGAGGCGACCATCTCGAGCTGAACGCTCTGTAGATGATCCTATTAGGGAGATGGAA GGCATGCTTGTTGATGAGTATGGAAG
Genomic sequence	ATTGTGGAGCTTCTTGTGCTGAAATTAGAGAATGAGCCTAATTTGCATCGAAGAGTTGACCTCC TTTTTCTTATAGACTCCATAACTCAGTGTTCTCACTCTCAGAAAGGTAATGTGCTATCCTCAGA ATTATTTGTTCTTAACAAGCTAATGTGTAGTAATTTATCTGAATTCTGAAACGTTTAGGTGTTG CTGGCGTTTCATATGTTCCCACCGTTCAAGCTGCGCTACCTCGTCTCTTGGGCGCTGCTGCTCC ACCAGGACCCGGTGCTCGTGAAAATCGACGACAGTGCCTCAAAGTAATGACTTAATTACATGTT CTTATTTTACTTCCTAATGGTCAATCATTTCTATCTTCTGGCTTTATAGGTTTTACGGTTATGG CTTGAGAGAAAGATTATGCCAGAAGGCATACTTCGAAGATACATGGATGATATTGAGGTGCCAA ATGATGATGCTAACACAGGTTTTCTGCTGAGGCGACCATCTCGAGCTGAACGCTCTGTAGATGA TCCTATTAGGGAGATGGAAGGCATGCTTGTTGATGAGTATGGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_007476* osi_circ_018262 						
					
						PMCS
						0.570264273

Conservation Information
Conserved circRNAs	osi_circ_007476* osi_circ_018262
PMCS	0.570264273

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							40997-40615(+)
41106-40628(+)
40681-41116(-)
						
						Potential amino acid sequence
						
							MPEGILRRYMDDIEVPNDDANTGFLLRRPSRAERSVDDPIREMEGMLVDEYGRLWSFLC*(+)
MILLGRWKACLLMSMEDCGASCAEIRE*(+)
MESIRKRRSTLRCKLGSFSNFSTRSSTIFHTHQQACLPSP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	40997-40615(+) 41106-40628(+) 40681-41116(-)
Potential amino acid sequence	MPEGILRRYMDDIEVPNDDANTGFLLRRPSRAERSVDDPIREMEGMLVDEYGRLWSFLC(+) MILLGRWKACLLMSMEDCGASCAEIRE(+) MESIRKRRSTLRCKLGSFSNFSTRSSTIFHTHQQACLPSP*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os08g0100500_circ_g.2

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