Plant Circular RNA Database

Detail information of GLYMA_20G067300_circ_g.1

			
						 General Information
					
						CircRNA Name
						GLYMA_20G067300_circ_g.1
					
						ID in PlantcircBase
						gma_circ_007748
					
						Alias
						gma-circRNA2722
					
						Organism
						
							Glycine max						
					
						Position
						
							chr20: 24098100-24099278  JBrowse»						
					
						Reference genome
						
							v2.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							GLYMA_20G067300						
					
						Parent gene annotation
						
							hypothetical protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						flower bud
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						KRG90096:2

General Information
CircRNA Name	GLYMA_20G067300_circ_g.1
ID in PlantcircBase	gma_circ_007748
Alias	gma-circRNA2722
Organism	Glycine max
Position	chr20: 24098100-24099278 JBrowse»
Reference genome	v2.0.38
Type	e-circRNA
Identification method	find_circ
Parent gene	GLYMA_20G067300
Parent gene annotation	hypothetical protein
Parent gene strand	+
Alternative splicing	NA
Support reads	NA
Tissues	flower bud
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	KRG90096:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CGATCAGCTGCAACTGCCTTGGTGGGGTGTTCTCCTCGCATGTGCCATTGCTATATTCTTCACT
CTCCCCATTGGAATTATTACTGCAATCACAAATCAGggccctgcacgagaaggatgagcgacca
aagggagggttaacgcgatcgcagttcttcgtaatcgcatttttgtgcagcttcgcctattacg
tgttcccc

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 826
Transcript exons: 24098100-24098669,24099023-24099278
GGCCCTGCACGAGAAGGATGAGCGACCAAAGGGAGGGTTAACGCGATCGCAGTTCTTCGTAATC
GCATTTTTGTGCAGCTTCGCCTATTACGTGTTCCCCGGTTACATTTTCCAAATGCTAACCTCCC
TCTCTTGGATTTGCTGGTTATATCCCAACAATGTTCTGGCCCAACAACTTGGGTCGGGCCTAAA
CGGGCTTGGCATAGGAGCAATTGGGCTTGACTGGTCCACCATTTCGTCCTACCTCGGAAGCCCA
CTCGCAAGCCCATGGTTTGCCACTGCCAATGTCGCGGTGGGTTTCGTGTTTGTGATGTACGTTC
TGACTCCGCTGTGCTATTGGCTCGACTTGTACAACGCCAAAACCTTCCCCATTTTCTCGGACGA
ACTCTTCACGGAGAAAGGTCAAGTTTATAACATCACAGCCATAATAGACTCCAATTTCCACCTA
GACCTTGCGGCTTATGAGAGACAAGGTAGATTGTACATCAGCACTTTCTTTGCCATGACCTATG
GTGTTGGCTTTGCTGCACTCACCGCCACCATTATGCATGTTGCTCTCTTTCACGGAAGAGAAAT
ATGGGAGCAAAGTAAATCAAGTTTTAAGGAAAAGAGCGTGGACATTCACACGAAGCTTATGCGG
AGGTACAAACAGGTGCCTGAATGGTGGTTCGTGTGCATACTCATGGCAACCATTGCAGCCACCG
TCTTCACCTGCGAATACTACAACGATCAGCTGCAACTGCCTTGGTGGGGTGTTCTCCTCGCATG
TGCCATTGCTATATTCTTCACTCTCCCCATTGGAATTATTACTGCAATCACAAATCAG
						
					

						Genomic sequence
						
							GGCCCTGCACGAGAAGGATGAGCGACCAAAGGGAGGGTTAACGCGATCGCAGTTCTTCGTAATC
GCATTTTTGTGCAGCTTCGCCTATTACGTGTTCCCCGGTTACATTTTCCAAATGCTAACCTCCC
TCTCTTGGATTTGCTGGTTATATCCCAACAATGTTCTGGCCCAACAACTTGGGTCGGGCCTAAA
CGGGCTTGGCATAGGAGCAATTGGGCTTGACTGGTCCACCATTTCGTCCTACCTCGGAAGCCCA
CTCGCAAGCCCATGGTTTGCCACTGCCAATGTCGCGGTGGGTTTCGTGTTTGTGATGTACGTTC
TGACTCCGCTGTGCTATTGGCTCGACTTGTACAACGCCAAAACCTTCCCCATTTTCTCGGACGA
ACTCTTCACGGAGAAAGGTCAAGTTTATAACATCACAGCCATAATAGACTCCAATTTCCACCTA
GACCTTGCGGCTTATGAGAGACAAGGTAGATTGTACATCAGCACTTTCTTTGCCATGACCTATG
GTGTTGGCTTTGCTGCACTCACCGCCACCATTATGCATGTTGCTCTCTTTCACGGAAGGTACGT
ACATCTGTACCAATCTCATGAAATAATATATAATTAATTAATTTAAAATTTCATATTGATTTTT
AGTTGTTTTTCTTAAAGATAAATTATATTTTTAATCCTTGTACTTCTAATTAATCTCTATACTT
TTTTAAAAAAATCAAGTGTATTTAGTCTCTTACATTTCACCTATTTAATACAAAAATTAAAATT
AAGTTTGAACGAAAATATACGAGAAAAAAATATGTTTTATCTTCTTTTTAATGTGTATATATGG
TCATGGATACAATTACATTTGACTAGATAGCTAGAGCATAGCGTGTATACACACATGACACATA
CATTAAATTGAAAATATATGTATACAGAGAAATATGGGAGCAAAGTAAATCAAGTTTTAAGGAA
AAGAGCGTGGACATTCACACGAAGCTTATGCGGAGGTACAAACAGGTGCCTGAATGGTGGTTCG
TGTGCATACTCATGGCAACCATTGCAGCCACCGTCTTCACCTGCGAATACTACAACGATCAGCT
GCAACTGCCTTGGTGGGGTGTTCTCCTCGCATGTGCCATTGCTATATTCTTCACTCTCCCCATT
GGAATTATTACTGCAATCACAAATCAG
						
					

			
		

Sequences
Splice junction sequence	CGATCAGCTGCAACTGCCTTGGTGGGGTGTTCTCCTCGCATGTGCCATTGCTATATTCTTCACT CTCCCCATTGGAATTATTACTGCAATCACAAATCAGggccctgcacgagaaggatgagcgacca aagggagggttaacgcgatcgcagttcttcgtaatcgcatttttgtgcagcttcgcctattacg tgttcccc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 826 Transcript exons: 24098100-24098669,24099023-24099278 GGCCCTGCACGAGAAGGATGAGCGACCAAAGGGAGGGTTAACGCGATCGCAGTTCTTCGTAATC GCATTTTTGTGCAGCTTCGCCTATTACGTGTTCCCCGGTTACATTTTCCAAATGCTAACCTCCC TCTCTTGGATTTGCTGGTTATATCCCAACAATGTTCTGGCCCAACAACTTGGGTCGGGCCTAAA CGGGCTTGGCATAGGAGCAATTGGGCTTGACTGGTCCACCATTTCGTCCTACCTCGGAAGCCCA CTCGCAAGCCCATGGTTTGCCACTGCCAATGTCGCGGTGGGTTTCGTGTTTGTGATGTACGTTC TGACTCCGCTGTGCTATTGGCTCGACTTGTACAACGCCAAAACCTTCCCCATTTTCTCGGACGA ACTCTTCACGGAGAAAGGTCAAGTTTATAACATCACAGCCATAATAGACTCCAATTTCCACCTA GACCTTGCGGCTTATGAGAGACAAGGTAGATTGTACATCAGCACTTTCTTTGCCATGACCTATG GTGTTGGCTTTGCTGCACTCACCGCCACCATTATGCATGTTGCTCTCTTTCACGGAAGAGAAAT ATGGGAGCAAAGTAAATCAAGTTTTAAGGAAAAGAGCGTGGACATTCACACGAAGCTTATGCGG AGGTACAAACAGGTGCCTGAATGGTGGTTCGTGTGCATACTCATGGCAACCATTGCAGCCACCG TCTTCACCTGCGAATACTACAACGATCAGCTGCAACTGCCTTGGTGGGGTGTTCTCCTCGCATG TGCCATTGCTATATTCTTCACTCTCCCCATTGGAATTATTACTGCAATCACAAATCAG
Genomic sequence	GGCCCTGCACGAGAAGGATGAGCGACCAAAGGGAGGGTTAACGCGATCGCAGTTCTTCGTAATC GCATTTTTGTGCAGCTTCGCCTATTACGTGTTCCCCGGTTACATTTTCCAAATGCTAACCTCCC TCTCTTGGATTTGCTGGTTATATCCCAACAATGTTCTGGCCCAACAACTTGGGTCGGGCCTAAA CGGGCTTGGCATAGGAGCAATTGGGCTTGACTGGTCCACCATTTCGTCCTACCTCGGAAGCCCA CTCGCAAGCCCATGGTTTGCCACTGCCAATGTCGCGGTGGGTTTCGTGTTTGTGATGTACGTTC TGACTCCGCTGTGCTATTGGCTCGACTTGTACAACGCCAAAACCTTCCCCATTTTCTCGGACGA ACTCTTCACGGAGAAAGGTCAAGTTTATAACATCACAGCCATAATAGACTCCAATTTCCACCTA GACCTTGCGGCTTATGAGAGACAAGGTAGATTGTACATCAGCACTTTCTTTGCCATGACCTATG GTGTTGGCTTTGCTGCACTCACCGCCACCATTATGCATGTTGCTCTCTTTCACGGAAGGTACGT ACATCTGTACCAATCTCATGAAATAATATATAATTAATTAATTTAAAATTTCATATTGATTTTT AGTTGTTTTTCTTAAAGATAAATTATATTTTTAATCCTTGTACTTCTAATTAATCTCTATACTT TTTTAAAAAAATCAAGTGTATTTAGTCTCTTACATTTCACCTATTTAATACAAAAATTAAAATT AAGTTTGAACGAAAATATACGAGAAAAAAATATGTTTTATCTTCTTTTTAATGTGTATATATGG TCATGGATACAATTACATTTGACTAGATAGCTAGAGCATAGCGTGTATACACACATGACACATA CATTAAATTGAAAATATATGTATACAGAGAAATATGGGAGCAAAGTAAATCAAGTTTTAAGGAA AAGAGCGTGGACATTCACACGAAGCTTATGCGGAGGTACAAACAGGTGCCTGAATGGTGGTTCG TGTGCATACTCATGGCAACCATTGCAGCCACCGTCTTCACCTGCGAATACTACAACGATCAGCT GCAACTGCCTTGGTGGGGTGTTCTCCTCGCATGTGCCATTGCTATATTCTTCACTCTCCCCATT GGAATTATTACTGCAATCACAAATCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.203367882

Conservation Information
Conserved circRNAs	NA
PMCS	0.203367882

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							24099114-24098137(+)
24098216-24098117(+)
24098610-24098117(+)
24098370-24099277(-)
						
						Potential amino acid sequence
						
							MVVRVHTHGNHCSHRLHLRILQRSAATALVGCSPRMCHCYILHSPHWNYYCNHKSGPCTRRMSD
QREG*(+)
MLTSLSWICWLYPNNVLAQQLGSGLNGLGIGAIGLDWSTISSYLGSPLASPWFATANVAVGFVF
VMYVLTPLCYWLDLYNAKTFPIFSDELFTEKGQVYNITAIIDSNFHLDLAAYERQGRLYISTFF
AMTYGVGFAALTATIMHVALFHGREIWEQSKSSFKEKSVDIHTKLMRRYKQVPEWWFVCILMAT
IAATVFTCEYYNDQLQLPWWGVLLACAIAIFFTLPIGIITAITNQGPAREG*(+)
MVLALLHSPPPLCMLLSFTEEKYGSKVNQVLRKRAWTFTRSLCGGTNRCLNGGSCAYSWQPLQP
PSSPANTTTISCNCLGGVFSSHVPLLYSSLSPLELLLQSQIRALHEKDERPKGGLTRSQFFVIA
FLCSFAYYVFPGYIFQMLTSLSWICWLYPNNVLAQQLGSGLNGLGIGAIGLDWSTISSYLGSPL
ASPWFATANVAVGFVFVMYVLTPLCYWLDLYNAKTFPIFSDELFTEKGQVYNITAIIDSNFHLD
LAAYERQGRLYISTFFAMTYGVGFAALTATIMHVALFHGREIWEQSKSSFKEKSVDIHTKLMRR
YKQVPEWWFVCILMATIAATVFTCEYYNDQLQLPWWGVLLACAIAIFFTLPIGIITAITNQGPA
REG*(+)
MGLRVGFRGRTKWWTSQAQLLLCQARLGPTQVVGPEHCWDITSKSKRGRLAFGKCNRGTRNRRS
CTKMRLRRTAIALTLPLVAHPSRAGP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	24099114-24098137(+) 24098216-24098117(+) 24098610-24098117(+) 24098370-24099277(-)
Potential amino acid sequence	MVVRVHTHGNHCSHRLHLRILQRSAATALVGCSPRMCHCYILHSPHWNYYCNHKSGPCTRRMSD QREG(+) MLTSLSWICWLYPNNVLAQQLGSGLNGLGIGAIGLDWSTISSYLGSPLASPWFATANVAVGFVF VMYVLTPLCYWLDLYNAKTFPIFSDELFTEKGQVYNITAIIDSNFHLDLAAYERQGRLYISTFF AMTYGVGFAALTATIMHVALFHGREIWEQSKSSFKEKSVDIHTKLMRRYKQVPEWWFVCILMAT IAATVFTCEYYNDQLQLPWWGVLLACAIAIFFTLPIGIITAITNQGPAREG(+) MVLALLHSPPPLCMLLSFTEEKYGSKVNQVLRKRAWTFTRSLCGGTNRCLNGGSCAYSWQPLQP PSSPANTTTISCNCLGGVFSSHVPLLYSSLSPLELLLQSQIRALHEKDERPKGGLTRSQFFVIA FLCSFAYYVFPGYIFQMLTSLSWICWLYPNNVLAQQLGSGLNGLGIGAIGLDWSTISSYLGSPL ASPWFATANVAVGFVFVMYVLTPLCYWLDLYNAKTFPIFSDELFTEKGQVYNITAIIDSNFHLD LAAYERQGRLYISTFFAMTYGVGFAALTATIMHVALFHGREIWEQSKSSFKEKSVDIHTKLMRR YKQVPEWWFVCILMATIAATVFTCEYYNDQLQLPWWGVLLACAIAIFFTLPIGIITAITNQGPA REG(+) MGLRVGFRGRTKWWTSQAQLLLCQARLGPTQVVGPEHCWDITSKSKRGRLAFGKCNRGTRNRRS CTKMRLRRTAIALTLPLVAHPSRAGP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chen et al., 2018

circRNA

Detail information of GLYMA_20G067300_circ_g.1

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