Plant Circular RNA Database

Detail information of orai.002G182700_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.002G182700_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000185
					
						Alias
						Chr02:48103908|48105841
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr02: 48103908-48105841  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.002G182700						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						ovule
					
						Exon boundary  
						
							No-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.002G182700.5:3
Gorai.002G182700.1:3
Gorai.002G182700.4:3
Gorai.002G182700.6:3
Gorai.002G182700.9:3
Gorai.002G182700.2:3
Gorai.002G182700.8:3
Gorai.002G182700.7:3
Gorai.002G182700.3:3

General Information
CircRNA Name	orai.002G182700_circ_g.1
ID in PlantcircBase	gra_circ_000185
Alias	Chr02:48103908\|48105841
Organism	Gossypium raimondii
Position	chrChr02: 48103908-48105841 JBrowse»
Reference genome	Graimondii_221
Type	ue-circRNA
Identification method	CIRI
Parent gene	Gorai.002G182700
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	2
Tissues	ovule
Exon boundary	No-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.002G182700.5:3 Gorai.002G182700.1:3 Gorai.002G182700.4:3 Gorai.002G182700.6:3 Gorai.002G182700.9:3 Gorai.002G182700.2:3 Gorai.002G182700.8:3 Gorai.002G182700.7:3 Gorai.002G182700.3:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GTTAATGGACAGCCAAACCTCCCTGGCAATCAGCTTTTGAATTCCATCACAATGCGATGTCATG
ATTCCAATGGATTCCTGCCCTCCAAGGGCAAGCAAAtgaagcatatttgaagtgtcatcagcaa
atgcttttagcgaaaaaattggggaaggggtgaacgaactactgttatgttaaagtggattgac
atttttct

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							TGAAGCATATTTGAAGTGTCATCAGCAAATGCTTTTAGCGAAAAAATTGGGGAAGGGGTGAACG
AACTACTGTTATGTTAAAGTGGATTGACATTTTTCTAAGTTGCACTCCTCTAGATCTGAAAAAC
AGAGATGATTGGACTCTGATGCTGTTGGATGTATAGGTAAAATTTGTTCTTTACATAACAGAAT
GATTTTGCATATTTTTGGTTTTGCATGCTCTGTATCCATATGGAGTCCACGAATGGTTTCCTTC
ATGTATAGTTGAATTTAGCATAATTGAATCTTCTTTGCCCGGTTTATTTCCTTTATAAATTGAA
CAAGCGTCAAAGGTTATACTTTCAGGATCTGGTGACAGGTTGCTTCTGTTTTATATGAAGACTG
TGCTTAGTGTTCTATGAGTTCTATCTCTCCGATGGATTGGAATGCCAAAACGCCTTTGCAATGG
GACTGGGAAAACTTGATGATGTTAAATGCAACACCCGCTGAAATTCCAAGGAAGCTCAGACCTG
AAGAGTGGGATATTGATGGAGAGGGAGGAATGAACTCAGGGTCTTTCTATTCTTCTGGTGCTGC
TGGAGGTAGTGGTGGTTCTGGCTCAGATTTGGGCCTTGCTTCTTTGTCTAAAAGTTCAAAATCG
GCATCTATTAATTCTTCTTCTATGGGGGAGGTAAAAATGACCAAATTTACTTCGGAAGCCTTTG
AAGCTATCCCAGATGATATTAGCAACCAGAACGAAGCTTTCAGGGCTGAGCTGGCTGGTACTTC
CCCAACACTTGAGGCTTCAGTTGGCTCTGGTGAGCCATTGCTCAGTTTAAAGCTTGGTAAACAG
ACATACTTTGAGGATGTTTCTGGAGGAAGCAATACTAAGAATTTGTCTTATTCTTCCACTTCTG
GACAATCTCCTGCCCAAGCAAAGAGATCTAAACCCAATTGTCAGGGCATACATGTTCCGCGTTG
CCAAGTTGAAGACTGTAACTTTGACCTCTCCTCAGCGAAGGATTACCATCGGAAACACAGAATT
TGTGAAAGTCATTCAAAGAGTCCAAAGGTCATTGTACGTGGTCAGGAACGCCGGTTCTGCCAAC
AGTGTAGCAAGTAAACTTTTTTCTTTATTCTGATGATTTTTACATTGGAAGTTGATTGTATCCA
AGAATATTTTCTTTATTGATTGGGTATTTGCATGGTGATGTTTTACTTATCTTAGTATCAAACA
GTAGTAGGTATTTGATTCCTTATTGTTGGGCTTGTGAACTGTTGATGAATGTTAAAATCATAAT
TATGTTAATGCTGTCAAAGGTTCCATGGCCTGTCGGAGTTTGATGAAAAGAAGAGAAGTTGTCG
CCGGCGCCTTTCTGACCACAATGCAAGACGCCGCAAACCACAGACAGAAGCATTCCACTTCAGT
GGTACAAGATTTTCATCTTCACCAAATGGTATCAGTTTCAGACATAGATTTGATGTTTTTTATG
CATATAATTTTGTTGTATGTTACTTGTCCTTCAAGATATTATGCAAGTGACTTAATTCATGCTT
ACTGAGTAGTTGCTTGATTTTATTCTATAACATCTTATACTCAAATAGTTTGGATCACCTTACT
TCATTGGATGCTATGTTTCTATTATAATTACTGTCCCAGCTTTCAAGTATATGTGTTAAGGCTT
TATAAGTTTTGCCTCTACTTGTTGCAGATGGGAAGCAACAGATGAGTTTCATCTGGAATAAAGT
GCCATTTCTTCATAATGCTAGACCCAATGAAATTTTTACATGGGAAGGCACATTTGATTCCAAG
TCCTCTCAAATGAAAGGTTATACGCCTACAAAAGTTGGAAATGTTAATGGACAGCCAAACCTCC
CTGGCAATCAGCTTTTGAATTCCATCACAATGCGATGTCATGATTCCAATGGATTCCTGCCCTC
CAAGGGCAAGCAAA
						
					

			
		

Sequences
Splice junction sequence	GTTAATGGACAGCCAAACCTCCCTGGCAATCAGCTTTTGAATTCCATCACAATGCGATGTCATG ATTCCAATGGATTCCTGCCCTCCAAGGGCAAGCAAAtgaagcatatttgaagtgtcatcagcaa atgcttttagcgaaaaaattggggaaggggtgaacgaactactgttatgttaaagtggattgac atttttct
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TGAAGCATATTTGAAGTGTCATCAGCAAATGCTTTTAGCGAAAAAATTGGGGAAGGGGTGAACG AACTACTGTTATGTTAAAGTGGATTGACATTTTTCTAAGTTGCACTCCTCTAGATCTGAAAAAC AGAGATGATTGGACTCTGATGCTGTTGGATGTATAGGTAAAATTTGTTCTTTACATAACAGAAT GATTTTGCATATTTTTGGTTTTGCATGCTCTGTATCCATATGGAGTCCACGAATGGTTTCCTTC ATGTATAGTTGAATTTAGCATAATTGAATCTTCTTTGCCCGGTTTATTTCCTTTATAAATTGAA CAAGCGTCAAAGGTTATACTTTCAGGATCTGGTGACAGGTTGCTTCTGTTTTATATGAAGACTG TGCTTAGTGTTCTATGAGTTCTATCTCTCCGATGGATTGGAATGCCAAAACGCCTTTGCAATGG GACTGGGAAAACTTGATGATGTTAAATGCAACACCCGCTGAAATTCCAAGGAAGCTCAGACCTG AAGAGTGGGATATTGATGGAGAGGGAGGAATGAACTCAGGGTCTTTCTATTCTTCTGGTGCTGC TGGAGGTAGTGGTGGTTCTGGCTCAGATTTGGGCCTTGCTTCTTTGTCTAAAAGTTCAAAATCG GCATCTATTAATTCTTCTTCTATGGGGGAGGTAAAAATGACCAAATTTACTTCGGAAGCCTTTG AAGCTATCCCAGATGATATTAGCAACCAGAACGAAGCTTTCAGGGCTGAGCTGGCTGGTACTTC CCCAACACTTGAGGCTTCAGTTGGCTCTGGTGAGCCATTGCTCAGTTTAAAGCTTGGTAAACAG ACATACTTTGAGGATGTTTCTGGAGGAAGCAATACTAAGAATTTGTCTTATTCTTCCACTTCTG GACAATCTCCTGCCCAAGCAAAGAGATCTAAACCCAATTGTCAGGGCATACATGTTCCGCGTTG CCAAGTTGAAGACTGTAACTTTGACCTCTCCTCAGCGAAGGATTACCATCGGAAACACAGAATT TGTGAAAGTCATTCAAAGAGTCCAAAGGTCATTGTACGTGGTCAGGAACGCCGGTTCTGCCAAC AGTGTAGCAAGTAAACTTTTTTCTTTATTCTGATGATTTTTACATTGGAAGTTGATTGTATCCA AGAATATTTTCTTTATTGATTGGGTATTTGCATGGTGATGTTTTACTTATCTTAGTATCAAACA GTAGTAGGTATTTGATTCCTTATTGTTGGGCTTGTGAACTGTTGATGAATGTTAAAATCATAAT TATGTTAATGCTGTCAAAGGTTCCATGGCCTGTCGGAGTTTGATGAAAAGAAGAGAAGTTGTCG CCGGCGCCTTTCTGACCACAATGCAAGACGCCGCAAACCACAGACAGAAGCATTCCACTTCAGT GGTACAAGATTTTCATCTTCACCAAATGGTATCAGTTTCAGACATAGATTTGATGTTTTTTATG CATATAATTTTGTTGTATGTTACTTGTCCTTCAAGATATTATGCAAGTGACTTAATTCATGCTT ACTGAGTAGTTGCTTGATTTTATTCTATAACATCTTATACTCAAATAGTTTGGATCACCTTACT TCATTGGATGCTATGTTTCTATTATAATTACTGTCCCAGCTTTCAAGTATATGTGTTAAGGCTT TATAAGTTTTGCCTCTACTTGTTGCAGATGGGAAGCAACAGATGAGTTTCATCTGGAATAAAGT GCCATTTCTTCATAATGCTAGACCCAATGAAATTTTTACATGGGAAGGCACATTTGATTCCAAG TCCTCTCAAATGAAAGGTTATACGCCTACAAAAGTTGGAAATGTTAATGGACAGCCAAACCTCC CTGGCAATCAGCTTTTGAATTCCATCACAATGCGATGTCATGATTCCAATGGATTCCTGCCCTC CAAGGGCAAGCAAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.102434074

Conservation Information
Conserved circRNAs	NA
PMCS	0.102434074

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							48104305-48103918(+)
48103930-48105803(-)
48103916-48105336(-)
						
						Potential amino acid sequence
						
							MSSISPMDWNAKTPLQWDWENLMMLNATPAEIPRKLRPEEWDIDGEGGMNSGSFYSSGAAGGSG
GSGSDLGLASLSKSSKSASINSSSMGEVKMTKFTSEAFEAIPDDISNQNEAFRAELAGTSPTLE
ASVGSGEPLLSLKLGKQTYFEDVSGGSNTKNLSYSSTSGQSPAQAKRSKPNCQGIHVPRCQVED
CNFDLSSAKDYHRKHRICESHSKSPKVIVRGQERRFCQQCSKFHGLSEFDEKKRSCRRRLSDHN
ARRRKPQTEAFHFSGTRFSSSPNDGKQQMSFIWNKVPFLHNARPNEIFTWEGTFDSKSSQMKGY
TPTKVGNVNGQPNLPGNQLLNSITMRCHDSNGFLPSKGKQMKHI*(+)
MTLQICFICLPLEGRNPLES*(-)
MLHLLALGGQESIGIMTSHCDGIQKLIAREVWLSINISNFCRRITFHLRGLGIKCAFPCKNFIG
SSIMKKWHFIPDETHLLLPIIW*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	48104305-48103918(+) 48103930-48105803(-) 48103916-48105336(-)
Potential amino acid sequence	MSSISPMDWNAKTPLQWDWENLMMLNATPAEIPRKLRPEEWDIDGEGGMNSGSFYSSGAAGGSG GSGSDLGLASLSKSSKSASINSSSMGEVKMTKFTSEAFEAIPDDISNQNEAFRAELAGTSPTLE ASVGSGEPLLSLKLGKQTYFEDVSGGSNTKNLSYSSTSGQSPAQAKRSKPNCQGIHVPRCQVED CNFDLSSAKDYHRKHRICESHSKSPKVIVRGQERRFCQQCSKFHGLSEFDEKKRSCRRRLSDHN ARRRKPQTEAFHFSGTRFSSSPNDGKQQMSFIWNKVPFLHNARPNEIFTWEGTFDSKSSQMKGY TPTKVGNVNGQPNLPGNQLLNSITMRCHDSNGFLPSKGKQMKHI(+) MTLQICFICLPLEGRNPLES(-) MLHLLALGGQESIGIMTSHCDGIQKLIAREVWLSINISNFCRRITFHLRGLGIKCAFPCKNFIG SSIMKKWHFIPDETHLLLPIIW*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.002G182700_circ_g.1

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