Plant Circular RNA Database

Detail information of Os01g0869900_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os01g0869900_circ_g.4
					
						ID in PlantcircBase
						osa_circ_004999
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 37711339-37712048  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						PcircRNA_finder, circRNA_finder
					
						Parent gene
						
							Os01g0869900						
					
						Parent gene annotation
						
							Serine/threonine protein kinase, Hyperosmotic stress response (O
s01t0869900-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0869900_circ_g.3 						
					
						Support reads
						13
					
						Tissues
						seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0869900-01:4

General Information
CircRNA Name	Os01g0869900_circ_g.4
ID in PlantcircBase	osa_circ_004999
Alias	NA
Organism	Oryza sativa
Position	chr1: 37711339-37712048 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	PcircRNA_finder, circRNA_finder
Parent gene	Os01g0869900
Parent gene annotation	Serine/threonine protein kinase, Hyperosmotic stress response (O s01t0869900-01)
Parent gene strand	+
Alternative splicing	Os01g0869900_circ_g.3
Support reads	13
Tissues	seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0869900-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGATCAGTCATCAGTATTGCATTCAAGACCCAAATCAGCAGTGGGGACGCCAGCATATATCGCA
CCAGAGGTGCTATCCCGCCGTGAGTATGATGGAAAGgtgatactgacgccaacgcatcttatga
ttgtcatggagttcgcagcaggcggggagctgttcgatcgaatctgtgatcgtggacggttcag
tgaggatg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 342
Transcript exons: 37711339-37711440,37711533-37711586,37711771-37711863,37
711956-37712048
GTGATACTGACGCCAACGCATCTTATGATTGTCATGGAGTTCGCAGCAGGCGGGGAGCTGTTCG
ATCGAATCTGTGATCGTGGACGGTTCAGTGAGGATGAGGCCAGGTATTTCTTTCAGCAGCTGAT
CTGTGGAGTGAGCTACTGCCATCACATGCAAATATGCCATAGAGATTTGAAGTTGGAGAATGTT
CTCTTGGATGGCAGCCCAGCTCCACGGCTTAAGATATGTGATTTTGGCTACTCCAAGTCATCAG
TATTGCATTCAAGACCCAAATCAGCAGTGGGGACGCCAGCATATATCGCACCAGAGGTGCTATC
CCGCCGTGAGTATGATGGAAAG
						
						Genomic sequence
						
							GTGATACTGACGCCAACGCATCTTATGATTGTCATGGAGTTCGCAGCAGGCGGGGAGCTGTTCG
ATCGAATCTGTGATCGTGGACGGTTCAGTGAGGATGAGGTCCCGTGATTTATTCCTGAGTTTTA
CTACTTTATAACCCTGTCTTATGTTGAACGAGCAATATTAATATTCTTTTATTCTGCTTGATGC
AGGCCAGGTATTTCTTTCAGCAGCTGATCTGTGGAGTGAGCTACTGCCATCACATGGTATGCAA
CAAACAGTTAACTCATTCAAGAGGACCAATTCACCATCTCTAAGCAATAATTACTACTGTACCC
TGTTTCAGTTTTATCATTCAGAGTGTTGGTAGTTTTCATTTGATTTTATAACTGAGCTAAATCT
TTATGAGTTTCTAATTATATTATTCTGGTGTCAATCTTCCAACTACAGCAAATATGCCATAGAG
ATTTGAAGTTGGAGAATGTTCTCTTGGATGGCAGCCCAGCTCCACGGCTTAAGATATGTGATTT
TGGCTACTCCAAGGTTTGTTGCCAAACCCACTGTCATTTTAGCAATCTTGGAGCTATTGGAATC
TCTGTAGATTCTCAGAATAATGTCTTTCCGTTCTTGATCAGTCATCAGTATTGCATTCAAGACC
CAAATCAGCAGTGGGGACGCCAGCATATATCGCACCAGAGGTGCTATCCCGCCGTGAGTATGAT
GGAAAG

Sequences
Splice junction sequence	TGATCAGTCATCAGTATTGCATTCAAGACCCAAATCAGCAGTGGGGACGCCAGCATATATCGCA CCAGAGGTGCTATCCCGCCGTGAGTATGATGGAAAGgtgatactgacgccaacgcatcttatga ttgtcatggagttcgcagcaggcggggagctgttcgatcgaatctgtgatcgtggacggttcag tgaggatg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 342 Transcript exons: 37711339-37711440,37711533-37711586,37711771-37711863,37 711956-37712048 GTGATACTGACGCCAACGCATCTTATGATTGTCATGGAGTTCGCAGCAGGCGGGGAGCTGTTCG ATCGAATCTGTGATCGTGGACGGTTCAGTGAGGATGAGGCCAGGTATTTCTTTCAGCAGCTGAT CTGTGGAGTGAGCTACTGCCATCACATGCAAATATGCCATAGAGATTTGAAGTTGGAGAATGTT CTCTTGGATGGCAGCCCAGCTCCACGGCTTAAGATATGTGATTTTGGCTACTCCAAGTCATCAG TATTGCATTCAAGACCCAAATCAGCAGTGGGGACGCCAGCATATATCGCACCAGAGGTGCTATC CCGCCGTGAGTATGATGGAAAG
Genomic sequence	GTGATACTGACGCCAACGCATCTTATGATTGTCATGGAGTTCGCAGCAGGCGGGGAGCTGTTCG ATCGAATCTGTGATCGTGGACGGTTCAGTGAGGATGAGGTCCCGTGATTTATTCCTGAGTTTTA CTACTTTATAACCCTGTCTTATGTTGAACGAGCAATATTAATATTCTTTTATTCTGCTTGATGC AGGCCAGGTATTTCTTTCAGCAGCTGATCTGTGGAGTGAGCTACTGCCATCACATGGTATGCAA CAAACAGTTAACTCATTCAAGAGGACCAATTCACCATCTCTAAGCAATAATTACTACTGTACCC TGTTTCAGTTTTATCATTCAGAGTGTTGGTAGTTTTCATTTGATTTTATAACTGAGCTAAATCT TTATGAGTTTCTAATTATATTATTCTGGTGTCAATCTTCCAACTACAGCAAATATGCCATAGAG ATTTGAAGTTGGAGAATGTTCTCTTGGATGGCAGCCCAGCTCCACGGCTTAAGATATGTGATTT TGGCTACTCCAAGGTTTGTTGCCAAACCCACTGTCATTTTAGCAATCTTGGAGCTATTGGAATC TCTGTAGATTCTCAGAATAATGTCTTTCCGTTCTTGATCAGTCATCAGTATTGCATTCAAGACC CAAATCAGCAGTGGGGACGCCAGCATATATCGCACCAGAGGTGCTATCCCGCCGTGAGTATGAT GGAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.341750493

Conservation Information
Conserved circRNAs	NA
PMCS	0.341750493

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							37711364-37712045(+)
37711803-37711339(+)
37711784-37712024(-)
37711375-37711856(-)
						
						Potential amino acid sequence
						
							MIVMEFAAGGELFDRICDRGRFSEDEARYFFQQLICGVSYCHHMQICHRDLKLENVLLDGSPAP
RLKICDFGYSKSSVLHSRPKSAVGTPAYIAPEVLSRREYDGKVILTPTHLMIVMEFAAGGELFD
RICDRGRFSEDEARYFFQQLICGVSYCHHMQICHRDLKLENVLLDGSPAPRLKICDFGYSKSSV
LHSRPKSAVGTPAYIAPEVLSRREYDGKVILTPTHLMIVMEFAAGGELFDRICDRGRFSEDEAR
YFFQQLICGVSYCHHMQICHRDLKLENVLLDGSPAPRLKICDFGYSKSSVLHSRPKSAVGTPAY
IAPEVLSRREYDGKVILTPTHLMIVMEFAAGGELFDRICDRGRFSEDEARYFFQQLICGVSYCH
HMQICHRDLKLENVLLDGSPAPRLKICDFGYSKSSVLHSRPKSAVGTPAYIAPEVLSRREYDGK
(+)
MFSWMAAQLHGLRYVILATPSHQYCIQDPNQQWGRQHISHQRCYPAVSMMER*(+)
MAYLHVMAVAHSTDQLLKEIPGLILTEPSTITDSIEQLPACCELHDNHKMRWRQYHLSIILTAG
*(-)
MTIIRCVGVSITFPSYSRRDSTSGAIYAGVPTADLGLECNTDDLE*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	37711364-37712045(+) 37711803-37711339(+) 37711784-37712024(-) 37711375-37711856(-)
Potential amino acid sequence	MIVMEFAAGGELFDRICDRGRFSEDEARYFFQQLICGVSYCHHMQICHRDLKLENVLLDGSPAP RLKICDFGYSKSSVLHSRPKSAVGTPAYIAPEVLSRREYDGKVILTPTHLMIVMEFAAGGELFD RICDRGRFSEDEARYFFQQLICGVSYCHHMQICHRDLKLENVLLDGSPAPRLKICDFGYSKSSV LHSRPKSAVGTPAYIAPEVLSRREYDGKVILTPTHLMIVMEFAAGGELFDRICDRGRFSEDEAR YFFQQLICGVSYCHHMQICHRDLKLENVLLDGSPAPRLKICDFGYSKSSVLHSRPKSAVGTPAY IAPEVLSRREYDGKVILTPTHLMIVMEFAAGGELFDRICDRGRFSEDEARYFFQQLICGVSYCH HMQICHRDLKLENVLLDGSPAPRLKICDFGYSKSSVLHSRPKSAVGTPAYIAPEVLSRREYDGK (+) MFSWMAAQLHGLRYVILATPSHQYCIQDPNQQWGRQHISHQRCYPAVSMMER(+) MAYLHVMAVAHSTDQLLKEIPGLILTEPSTITDSIEQLPACCELHDNHKMRWRQYHLSIILTAG (-) MTIIRCVGVSITFPSYSRRDSTSGAIYAGVPTADLGLECNTDDLE*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os01g0869900_circ_g.4

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