Plant Circular RNA Database

Detail information of Os02g0244700_circ_g.8

			
						 General Information
					
						CircRNA Name
						Os02g0244700_circ_g.8
					
						ID in PlantcircBase
						osa_circ_014061
					
						Alias
						Os_ciR2589
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 8178722-8179108  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os02g0244700						
					
						Parent gene annotation
						
							Similar to Phosphoenolpyruvate carboxylase 1 (EC 4.1.1.31) (PEPC
ase 1) (CP21). (Os02t0244700-01);Similar to Phosphoenolpyruvate 
carboxylase 1 (EC 4.1.1.31) (PEPCase 1) (CP21). (Os02t0244700-02
);Similar to Phosphoenolpyruvate carboxylase. (Os02t0244700-03);
Similar to Phosphoenolpyruvate carboxylase. (Os02t0244700-04)
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Os02g0244700_circ_g.7 Os02g0244700_circ_g.9 Os02g0244700_circ_g.10 Os02g0244700_circ_g.11 Os02g0244700_circ_g.12 Os02g0244700_circ_g.13 Os02g0244700_circ_g.14 Os02g0244700_circ_g.15 Os02g0244700_circ_g.16 Os02g0244700_circ_g.17 						
					
						Support reads
						6/3
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0244850-00:1
Os02t0244700-01:1
Os02t0244850-00:1
Os02t0244700-04:1
Os02t0244700-02:1

General Information
CircRNA Name	Os02g0244700_circ_g.8
ID in PlantcircBase	osa_circ_014061
Alias	Os_ciR2589
Organism	Oryza sativa
Position	chr2: 8178722-8179108 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os02g0244700
Parent gene annotation	Similar to Phosphoenolpyruvate carboxylase 1 (EC 4.1.1.31) (PEPC ase 1) (CP21). (Os02t0244700-01);Similar to Phosphoenolpyruvate carboxylase 1 (EC 4.1.1.31) (PEPCase 1) (CP21). (Os02t0244700-02 );Similar to Phosphoenolpyruvate carboxylase. (Os02t0244700-03); Similar to Phosphoenolpyruvate carboxylase. (Os02t0244700-04)
Parent gene strand	-
Alternative splicing	Os02g0244700_circ_g.7 Os02g0244700_circ_g.9 Os02g0244700_circ_g.10 Os02g0244700_circ_g.11 Os02g0244700_circ_g.12 Os02g0244700_circ_g.13 Os02g0244700_circ_g.14 Os02g0244700_circ_g.15 Os02g0244700_circ_g.16 Os02g0244700_circ_g.17
Support reads	6/3
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0244850-00:1 Os02t0244700-01:1 Os02t0244850-00:1 Os02t0244700-04:1 Os02t0244700-02:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCCAAGGAATTGCACGGAGCGATTCAATGCCACCACTAGGCTTTCTCTTTGATGGTCGGCTACC
AATGTTCATCCTGCCATATTCTGTCTCAGGTGTTGCctgaaggagtagctgttttgtctcttca
aagttgtttctcagctgctctccaaaggattgcagatcacctgccacaagcaatttgtcatata
aagcagca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 231
Transcript exons: 8178722-8178890,8179047-8179108
CTGAAGGAGTAGCTGTTTTGTCTCTTCAAAGTTGTTTCTCAGCTGCTCTCCAAAGGATTGCAGA
TCACCTGCCACAAGCAATTTGTCATATAAAGCAGCAATTCCTGGATCTCCCTTGGCGAAAACCA
TCTCAAGCAAGTCCAAGGTGACCCTGAAGAATGGCCACTCAGGCTTTCTCTTTGATGGTCGGCT
ACCAATGTTCATCCTGCCATATTCTGTCTCAGGTGTTGC
Transcript length: 387
Transcript exons: 8178722-8179108
CTGAAGGAGTAGCTGTTTTGTCTCTTCAAAGTTGTTTCTCAGCTGCTCTCCAAAGGATTGCAGA
TCACCTGCCACAAGCAATTTGTCATATAAAGCAGCAATTCCTGGATCTCCCTTGGCGAAAACCA
TCTCAAGCAAGTCCAAGGTGACCCTGAAGAATGGCCACTCATTGTACATTTCTTTCAGTGTATG
GATGTTCCTGATATCCTTCTGCATTATATGTTTGAACGCTCCACCAAATCCAAGCCATACAGGA
AGATGAAACCTAGTTTGTGTCCAAGCAAAAATCCAAGGAATTGCACGGAGCGATTCAATGCCAC
CACTAGGCTTTCTCTTTGATGGTCGGCTACCAATGTTCATCCTGCCATATTCTGTCTCAGGTGT
TGC
						
						Genomic sequence
						
							CTGAAGGAGTAGCTGTTTTGTCTCTTCAAAGTTGTTTCTCAGCTGCTCTCCAAAGGATTGCAGA
TCACCTGCCACAAGCAATTTGTCATATAAAGCAGCAATTCCTGGATCTCCCTTGGCGAAAACCA
TCTCAAGCAAGTCCAAGGTGACCCTGAAGAATGGCCACTCATTGTACATTTCTTTCAGTGTATG
GATGTTCCTGATATCCTTCTGCATTATATGTTTGAACGCTCCACCAAATCCAAGCCATACAGGA
AGATGAAACCTAGTTTGTGTCCAAGCAAAAATCCAAGGAATTGCACGGAGCGATTCAATGCCAC
CACTAGGCTTTCTCTTTGATGGTCGGCTACCAATGTTCATCCTGCCATATTCTGTCTCAGGTGT
TGC

Sequences
Splice junction sequence	TCCAAGGAATTGCACGGAGCGATTCAATGCCACCACTAGGCTTTCTCTTTGATGGTCGGCTACC AATGTTCATCCTGCCATATTCTGTCTCAGGTGTTGCctgaaggagtagctgttttgtctcttca aagttgtttctcagctgctctccaaaggattgcagatcacctgccacaagcaatttgtcatata aagcagca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 231 Transcript exons: 8178722-8178890,8179047-8179108 CTGAAGGAGTAGCTGTTTTGTCTCTTCAAAGTTGTTTCTCAGCTGCTCTCCAAAGGATTGCAGA TCACCTGCCACAAGCAATTTGTCATATAAAGCAGCAATTCCTGGATCTCCCTTGGCGAAAACCA TCTCAAGCAAGTCCAAGGTGACCCTGAAGAATGGCCACTCAGGCTTTCTCTTTGATGGTCGGCT ACCAATGTTCATCCTGCCATATTCTGTCTCAGGTGTTGC Transcript length: 387 Transcript exons: 8178722-8179108 CTGAAGGAGTAGCTGTTTTGTCTCTTCAAAGTTGTTTCTCAGCTGCTCTCCAAAGGATTGCAGA TCACCTGCCACAAGCAATTTGTCATATAAAGCAGCAATTCCTGGATCTCCCTTGGCGAAAACCA TCTCAAGCAAGTCCAAGGTGACCCTGAAGAATGGCCACTCATTGTACATTTCTTTCAGTGTATG GATGTTCCTGATATCCTTCTGCATTATATGTTTGAACGCTCCACCAAATCCAAGCCATACAGGA AGATGAAACCTAGTTTGTGTCCAAGCAAAAATCCAAGGAATTGCACGGAGCGATTCAATGCCAC CACTAGGCTTTCTCTTTGATGGTCGGCTACCAATGTTCATCCTGCCATATTCTGTCTCAGGTGT TGC
Genomic sequence	CTGAAGGAGTAGCTGTTTTGTCTCTTCAAAGTTGTTTCTCAGCTGCTCTCCAAAGGATTGCAGA TCACCTGCCACAAGCAATTTGTCATATAAAGCAGCAATTCCTGGATCTCCCTTGGCGAAAACCA TCTCAAGCAAGTCCAAGGTGACCCTGAAGAATGGCCACTCATTGTACATTTCTTTCAGTGTATG GATGTTCCTGATATCCTTCTGCATTATATGTTTGAACGCTCCACCAAATCCAAGCCATACAGGA AGATGAAACCTAGTTTGTGTCCAAGCAAAAATCCAAGGAATTGCACGGAGCGATTCAATGCCAC CACTAGGCTTTCTCTTTGATGGTCGGCTACCAATGTTCATCCTGCCATATTCTGTCTCAGGTGT TGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.656275366

Conservation Information
Conserved circRNAs	NA
PMCS	0.656275366

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8179036-8178722(+)
8179061-8178867(+)
8179083-8178724(-)
						
						Potential amino acid sequence
						
							MPPLGFLFDGRLPMFILPYSVSGVA*(+)
MVGYQCSSCHILSQVLPEGVAVLSLQSCFSAALQRIADHLPQAICHIKQQFLDLPWRKPSQASP
R*(+)
MNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKEMYN
EWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQATPETEYG
RMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKEMY
NEWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQATPETEY
GRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKEM
YNEWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQATPETE
YGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKE
MYNEWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQ(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8179036-8178722(+) 8179061-8178867(+) 8179083-8178724(-)
Potential amino acid sequence	MPPLGFLFDGRLPMFILPYSVSGVA(+) MVGYQCSSCHILSQVLPEGVAVLSLQSCFSAALQRIADHLPQAICHIKQQFLDLPWRKPSQASP R(+) MNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKEMYN EWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQATPETEYG RMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKEMY NEWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQATPETEY GRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKEM YNEWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQATPETE YGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGAFKHIMQKDIRNIHTLKE MYNEWPFFRVTLDLLEMVFAKGDPGIAALYDKLLVAGDLQSFGEQLRNNFEETKQLLLQ(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0244700_circ_g.8

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