Plant Circular RNA Database

Detail information of orai.010G060800_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.010G060800_circ_g.1
					
						ID in PlantcircBase
						gra_circ_001093
					
						Alias
						Chr10:7352016|7353653
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr10: 7352016-7353653  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.010G060800						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.010G060800.4:7
Gorai.010G060800.5:4
Gorai.010G060800.7:7
Gorai.010G060800.2:7
Gorai.010G060800.1:7
Gorai.010G060800.3:6
Gorai.010G060800.6:7

General Information
CircRNA Name	orai.010G060800_circ_g.1
ID in PlantcircBase	gra_circ_001093
Alias	Chr10:7352016\|7353653
Organism	Gossypium raimondii
Position	chrChr10: 7352016-7353653 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.010G060800
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	2
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.010G060800.4:7 Gorai.010G060800.5:4 Gorai.010G060800.7:7 Gorai.010G060800.2:7 Gorai.010G060800.1:7 Gorai.010G060800.3:6 Gorai.010G060800.6:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GGGTGCTCTTGTTTTTTTGGCTAGGGTTTAAATGATCCTGTCCCAGTTGCTTCAGAATTAGACG
AGAAGAATGCCTTGGCATTGGCTATTGTTCCTGTTGccacaaggtgcagctgttcataattttg
ttatccggtttgcactttcaatggtatgttactttttaaacatactaccactctccatatttgt
agagtgaa

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CCACAAGGTGCAGCTGTTCATAATTTTGTTATCCGGTTTGCACTTTCAATGGTATGTTACTTTT
TAAACATACTACCACTCTCCATATTTGTAGAGTGAATTTGGAGATGACATTTTCTTCTTTTTGT
CCTTTTGTCATTTCAAACAGGTTGCTTCCGAAAGTATCAAAATTTATCAAGCTATAAGTGATGG
TACAGTCAATCTTGTAGACAAGGTAAGCTGTGGTCTTAATCTAAATAAGCTGCTTTTCTATTTA
GTGAACCATGTGTATTTGATGATCTGAACAATGACAGTTCTTTGAGATGCAACGTCCTGATGCT
TTGAAGGCTTTGGACATATACCGGAGATCTGGGCAACAGGTAAATTGCTGTGCCAATATTTGCT
AAAGTCATCTCCTTTTGTGAATGAGGTGGATTTCAACTCTGGAGGAATTTAACACTTTCTGTTT
TCAGGCAGAGAGGCTTTCAGAATTTTACGAAGTATGTAAAAGTCTGGATGTAGGGCGTGGAGAA
AGGTTTATTAAGATAGAGCAGGTTAGGATCCTTCACCTTTGGTGCCTTCTGAAACTGATTTACT
TGTCCTTGAATTCTAGTCACATGTCATCATAAAACTGATTTTCTTTTGGTGTCTATGCTGAAGC
CTCCTGCATCGTTTCTACAAGCCATGGAAGAGTATGTAAGAGAAGCTCCACGGTCTTCAGCAGT
TCGCAAAGATCAGGTCTTGAAATTCTGTGGAATTGGTTTTTGTTTGAACAAGAAGATAATTTAT
TTTATTTTATTTCATTTTATATATTTCTATGCCTTTGTGATATAAGATCTGTATGTATTTGTAA
ACATCATCCTGAATACCTTATGAATTTATAAAAATTGTTTTATTTGATTGATTTGTGGAAAGTA
AAGCAATCTGATTTTTAGTTTGTTATGAAAAGTAAGCCAAGAGAATGATGTTTGCCCATTTCCT
TGAATGAATGATGATTCACCCATTTGTGCAGATCTAAACAATTACATTTTAAAAAAAATTAAAA
TATGAGGATCAACCAATGTTCATTCTTCCATCCATTGAGTTATTTCTCATATATAGTAGTCTAT
TACCATCTCTATGATATACGTTGTGATTATTTACATAAAAGTCCTGCTTGAAGTTTTACTACTT
TCTGTTTGTTTTACTAAAGTTTGCAAGTATTGCTGTTAATACTTTTTATTTTTGAACTAGCAGG
CTGACAAACCCAAGGAAGTGTTGGCCATTGAGTACAAGAAAACCCCAGAGGAGCAGGAGAAACG
TTCACCATCACCTCCTCCTCCTGAACCAGAAAAAGTGGAGAAAGTGGAAGAGCCTATTGTTGAA
CCTCCTGATTTGTTGGTAACTTCCAGTCTTTTTTCTTTTGTTGTTACCGACTATATTGTTATAT
GAGAACTTGGCACATCATTTTTTCTAGTTTAATGATGAACTTTGTAGTCTCTTTTTGTTCTTGC
TACCTACTATAGTTTTATAAGAGAAATTGGCAGTTCATATGCTTTTTTCTCTAACCAGTTTCAT
TTGGGTGCTCTTGTTTTTTTGGCTAGGGTTTAAATGATCCTGTCCCAGTTGCTTCAGAATTAGA
CGAGAAGAATGCCTTGGCATTGGCTATTGTTCCTGTTG
						
					

			
		

Sequences
Splice junction sequence	GGGTGCTCTTGTTTTTTTGGCTAGGGTTTAAATGATCCTGTCCCAGTTGCTTCAGAATTAGACG AGAAGAATGCCTTGGCATTGGCTATTGTTCCTGTTGccacaaggtgcagctgttcataattttg ttatccggtttgcactttcaatggtatgttactttttaaacatactaccactctccatatttgt agagtgaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CCACAAGGTGCAGCTGTTCATAATTTTGTTATCCGGTTTGCACTTTCAATGGTATGTTACTTTT TAAACATACTACCACTCTCCATATTTGTAGAGTGAATTTGGAGATGACATTTTCTTCTTTTTGT CCTTTTGTCATTTCAAACAGGTTGCTTCCGAAAGTATCAAAATTTATCAAGCTATAAGTGATGG TACAGTCAATCTTGTAGACAAGGTAAGCTGTGGTCTTAATCTAAATAAGCTGCTTTTCTATTTA GTGAACCATGTGTATTTGATGATCTGAACAATGACAGTTCTTTGAGATGCAACGTCCTGATGCT TTGAAGGCTTTGGACATATACCGGAGATCTGGGCAACAGGTAAATTGCTGTGCCAATATTTGCT AAAGTCATCTCCTTTTGTGAATGAGGTGGATTTCAACTCTGGAGGAATTTAACACTTTCTGTTT TCAGGCAGAGAGGCTTTCAGAATTTTACGAAGTATGTAAAAGTCTGGATGTAGGGCGTGGAGAA AGGTTTATTAAGATAGAGCAGGTTAGGATCCTTCACCTTTGGTGCCTTCTGAAACTGATTTACT TGTCCTTGAATTCTAGTCACATGTCATCATAAAACTGATTTTCTTTTGGTGTCTATGCTGAAGC CTCCTGCATCGTTTCTACAAGCCATGGAAGAGTATGTAAGAGAAGCTCCACGGTCTTCAGCAGT TCGCAAAGATCAGGTCTTGAAATTCTGTGGAATTGGTTTTTGTTTGAACAAGAAGATAATTTAT TTTATTTTATTTCATTTTATATATTTCTATGCCTTTGTGATATAAGATCTGTATGTATTTGTAA ACATCATCCTGAATACCTTATGAATTTATAAAAATTGTTTTATTTGATTGATTTGTGGAAAGTA AAGCAATCTGATTTTTAGTTTGTTATGAAAAGTAAGCCAAGAGAATGATGTTTGCCCATTTCCT TGAATGAATGATGATTCACCCATTTGTGCAGATCTAAACAATTACATTTTAAAAAAAATTAAAA TATGAGGATCAACCAATGTTCATTCTTCCATCCATTGAGTTATTTCTCATATATAGTAGTCTAT TACCATCTCTATGATATACGTTGTGATTATTTACATAAAAGTCCTGCTTGAAGTTTTACTACTT TCTGTTTGTTTTACTAAAGTTTGCAAGTATTGCTGTTAATACTTTTTATTTTTGAACTAGCAGG CTGACAAACCCAAGGAAGTGTTGGCCATTGAGTACAAGAAAACCCCAGAGGAGCAGGAGAAACG TTCACCATCACCTCCTCCTCCTGAACCAGAAAAAGTGGAGAAAGTGGAAGAGCCTATTGTTGAA CCTCCTGATTTGTTGGTAACTTCCAGTCTTTTTTCTTTTGTTGTTACCGACTATATTGTTATAT GAGAACTTGGCACATCATTTTTTCTAGTTTAATGATGAACTTTGTAGTCTCTTTTTGTTCTTGC TACCTACTATAGTTTTATAAGAGAAATTGGCAGTTCATATGCTTTTTTCTCTAACCAGTTTCAT TTGGGTGCTCTTGTTTTTTTGGCTAGGGTTTAAATGATCCTGTCCCAGTTGCTTCAGAATTAGA CGAGAAGAATGCCTTGGCATTGGCTATTGTTCCTGTTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.105094078

Conservation Information
Conserved circRNAs	NA
PMCS	0.105094078

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7352064-7352035(+)
7353624-7352035(+)
						
						Potential amino acid sequence
						
							MVASESIKIYQAISDGTVNLVDKFFEMQRPDALKALDIYRRSGQQAERLSEFYEVCKSLDVGRG
ERFIKIEQPPASFLQAMEEYVREAPRSSAVRKDQADKPKEVLAIEYKKTPEEQEKRSPSPPPPE
PEKVEKVEEPIVEPPDLLGLNDPVPVASELDEKNALALAIVPVATRCSCS*(+)
MPWHWLLFLLPQGAAVHNFVIRFALSMVASESIKIYQAISDGTVNLVDKFFEMQRPDALKALDI
YRRSGQQAERLSEFYEVCKSLDVGRGERFIKIEQPPASFLQAMEEYVREAPRSSAVRKDQADKP
KEVLAIEYKKTPEEQEKRSPSPPPPEPEKVEKVEEPIVEPPDLLGLNDPVPVASELDEKNALAL
AIVPVATRCSCS*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	7352064-7352035(+) 7353624-7352035(+)
Potential amino acid sequence	MVASESIKIYQAISDGTVNLVDKFFEMQRPDALKALDIYRRSGQQAERLSEFYEVCKSLDVGRG ERFIKIEQPPASFLQAMEEYVREAPRSSAVRKDQADKPKEVLAIEYKKTPEEQEKRSPSPPPPE PEKVEKVEEPIVEPPDLLGLNDPVPVASELDEKNALALAIVPVATRCSCS(+) MPWHWLLFLLPQGAAVHNFVIRFALSMVASESIKIYQAISDGTVNLVDKFFEMQRPDALKALDI YRRSGQQAERLSEFYEVCKSLDVGRGERFIKIEQPPASFLQAMEEYVREAPRSSAVRKDQADKP KEVLAIEYKKTPEEQEKRSPSPPPPEPEKVEKVEEPIVEPPDLLGLNDPVPVASELDEKNALAL AIVPVATRCSCS(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.010G060800_circ_g.1

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