Plant Circular RNA Database

Detail information of Os01g0239100_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os01g0239100_circ_g.1
					
						ID in PlantcircBase
						osa_circ_001012
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 7719227-7720225  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os01g0239100						
					
						Parent gene annotation
						
							Heat shock protein DnaJ family protein. (Os01t0239100-01);Heat s
hock protein DnaJ family protein. (Os01t0239100-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0239100_circ_g.2 						
					
						Support reads
						2
					
						Tissues
						seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0239200-01:5
Os01t0239200-01:5
Os01t0239100-02:1

General Information
CircRNA Name	Os01g0239100_circ_g.1
ID in PlantcircBase	osa_circ_001012
Alias	NA
Organism	Oryza sativa
Position	chr1: 7719227-7720225 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os01g0239100
Parent gene annotation	Heat shock protein DnaJ family protein. (Os01t0239100-01);Heat s hock protein DnaJ family protein. (Os01t0239100-02)
Parent gene strand	+
Alternative splicing	Os01g0239100_circ_g.2
Support reads	2
Tissues	seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0239200-01:5 Os01t0239200-01:5 Os01t0239100-02:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GAATAAATGCTCCGGTAAGTGAATGAGATGTTAGAGATCATAGCATTGATGAAGCCCGTCCAGT
TAAATGAAAGTTCAGTCAGTGATGCCATTGAAACACccttttctcctcttcgatcttagccttg
atgtacgagtagatggcaacaccagctatggcaatgcaagtaccaattccagtctgtgtggtaa
ttctgttg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 509
Transcript exons: 7719227-7719327,7719449-7719548,7719706-7719822,7719913-
7719990,7720113-7720225
CCTTTTCTCCTCTTCGATCTTAGCCTTGATGTACGAGTAGATGGCAACACCAGCTATGGCAATG
CAAGTACCAATTCCAGTCTGTGTGGTAATTCTGTTGCCAAAAACAATGATGGAGAATCCAATGA
CGAAAACCCTTTTCAACACATTGCCAACGGCATGCGTCAGAGGGGCCACTCGCTCCAGAGTGTT
TGTAGCAACCTGGTTATAGAGGTGGTAGAACAGTCCCACGAAGAAAAGGTCAGAAACGAACTTT
GTCAAGCCTACTTTTGCAATTGCATCGTTAAAACCATGCTGCAATAGTTGAGGTCCCTCGATGA
TCACTGCTGGTGGAATGCACACAATAAGGGCAATTATTGAGATATAAGCATACACATTGGTGCT
ATCCATATCAGTCATAGCTTTCTTGGAATAAATGCTCCGGTAAGTGAATGAGATGTTAGAGATC
ATAGCATTGATGAAGCCCGTCCAGTTAAATGAAAGTTCAGTCAGTGATGCCATTGAAACAC
						
					

						Genomic sequence
						
							CCTTTTCTCCTCTTCGATCTTAGCCTTGATGTACGAGTAGATGGCAACACCAGCTATGGCAATG
CAAGTACCAATTCCAGTCTGTGTGGTAATTCTGTTGCCTGTTTAACATATGATTTACCAAGTGT
TAGGCTAGGCATAAACACATCAAACAAAAAATGCAACGAACATAACAAATTTGTAATTTTCAAT
TCGTAAAGGCATGGGGGAAAAATTACTTACCAAAAACAATGATGGAGAATCCAATGACGAAAAC
CCTTTTCAACACATTGCCAACGGCATGCGTCAGAGGGGCCACTCGCTCCAGAGTGTTTGTAGCA
ACCTGCATGACATGATTGCATTAGTGATGTTCTAGTAGATGAAAGGTAGTCAATGAAAAAATTG
GAATTTGTTTACATAAGAATGAAACTTAGGTACATCCTGTGAAGCACAAAAATACGATGAATTT
GGTGGTTTTGCATGGCATGAAAACGGGTTACCTGGTTATAGAGGTGGTAGAACAGTCCCACGAA
GAAAAGGTCAGAAACGAACTTTGTCAAGCCTACTTTTGCAATTGCATCGTTAAAACCATGCTGC
AATAGTTGAGGTCCCTCGATCTGCAAGTTGGTCATGGAAAGAGGAACACCAACAGTAAGCATAA
TGCACGAATAATATTATACTTCTTGGACCAAATTTATCACACTTACGATCACTGCTGGTGGAAT
GCACACAATAAGGGCAATTATTGAGATATAAGCATACACATTGGTGCTATCCATATCAGTCTGC
AACAGTGAAAATGGTTTTTTAACGTCAGAAAGGACAGAATCAGGAATCAACGCAAAATCCCATG
AGGCAAAAAAAAAAAGGTGATGAATCAACAGATTAGCACAAAAGAGGCACAAACCATAGCTTTC
TTGGAATAAATGCTCCGGTAAGTGAATGAGATGTTAGAGATCATAGCATTGATGAAGCCCGTCC
AGTTAAATGAAAGTTCAGTCAGTGATGCCATTGAAACAC
						
					

			
		

Sequences
Splice junction sequence	GAATAAATGCTCCGGTAAGTGAATGAGATGTTAGAGATCATAGCATTGATGAAGCCCGTCCAGT TAAATGAAAGTTCAGTCAGTGATGCCATTGAAACACccttttctcctcttcgatcttagccttg atgtacgagtagatggcaacaccagctatggcaatgcaagtaccaattccagtctgtgtggtaa ttctgttg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 509 Transcript exons: 7719227-7719327,7719449-7719548,7719706-7719822,7719913- 7719990,7720113-7720225 CCTTTTCTCCTCTTCGATCTTAGCCTTGATGTACGAGTAGATGGCAACACCAGCTATGGCAATG CAAGTACCAATTCCAGTCTGTGTGGTAATTCTGTTGCCAAAAACAATGATGGAGAATCCAATGA CGAAAACCCTTTTCAACACATTGCCAACGGCATGCGTCAGAGGGGCCACTCGCTCCAGAGTGTT TGTAGCAACCTGGTTATAGAGGTGGTAGAACAGTCCCACGAAGAAAAGGTCAGAAACGAACTTT GTCAAGCCTACTTTTGCAATTGCATCGTTAAAACCATGCTGCAATAGTTGAGGTCCCTCGATGA TCACTGCTGGTGGAATGCACACAATAAGGGCAATTATTGAGATATAAGCATACACATTGGTGCT ATCCATATCAGTCATAGCTTTCTTGGAATAAATGCTCCGGTAAGTGAATGAGATGTTAGAGATC ATAGCATTGATGAAGCCCGTCCAGTTAAATGAAAGTTCAGTCAGTGATGCCATTGAAACAC
Genomic sequence	CCTTTTCTCCTCTTCGATCTTAGCCTTGATGTACGAGTAGATGGCAACACCAGCTATGGCAATG CAAGTACCAATTCCAGTCTGTGTGGTAATTCTGTTGCCTGTTTAACATATGATTTACCAAGTGT TAGGCTAGGCATAAACACATCAAACAAAAAATGCAACGAACATAACAAATTTGTAATTTTCAAT TCGTAAAGGCATGGGGGAAAAATTACTTACCAAAAACAATGATGGAGAATCCAATGACGAAAAC CCTTTTCAACACATTGCCAACGGCATGCGTCAGAGGGGCCACTCGCTCCAGAGTGTTTGTAGCA ACCTGCATGACATGATTGCATTAGTGATGTTCTAGTAGATGAAAGGTAGTCAATGAAAAAATTG GAATTTGTTTACATAAGAATGAAACTTAGGTACATCCTGTGAAGCACAAAAATACGATGAATTT GGTGGTTTTGCATGGCATGAAAACGGGTTACCTGGTTATAGAGGTGGTAGAACAGTCCCACGAA GAAAAGGTCAGAAACGAACTTTGTCAAGCCTACTTTTGCAATTGCATCGTTAAAACCATGCTGC AATAGTTGAGGTCCCTCGATCTGCAAGTTGGTCATGGAAAGAGGAACACCAACAGTAAGCATAA TGCACGAATAATATTATACTTCTTGGACCAAATTTATCACACTTACGATCACTGCTGGTGGAAT GCACACAATAAGGGCAATTATTGAGATATAAGCATACACATTGGTGCTATCCATATCAGTCTGC AACAGTGAAAATGGTTTTTTAACGTCAGAAAGGACAGAATCAGGAATCAACGCAAAATCCCATG AGGCAAAAAAAAAAAGGTGATGAATCAACAGATTAGCACAAAAGAGGCACAAACCATAGCTTTC TTGGAATAAATGCTCCGGTAAGTGAATGAGATGTTAGAGATCATAGCATTGATGAAGCCCGTCC AGTTAAATGAAAGTTCAGTCAGTGATGCCATTGAAACAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.285485002

Conservation Information
Conserved circRNAs	NA
PMCS	0.285485002

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7720154-7719246(+)
7720194-7719804(+)
7720219-7720207(-)
						
						Potential amino acid sequence
						
							MLEIIALMKPVQLNESSVSDAIETPFSPLRS*(+)
MKVQSVMPLKHPFLLFDLSLDVRVDGNTSYGNASTNSSLCGNSVAKNNDGESNDENPFQHIANG
MRQRGHSLQSVCSNLVIEVVEQSHEEKVRNELCQAYFCNCIVKTMLQ*(+)
MASLTELSFNWTGFINAMISNISFTYRSIYSKKAMTDMDSTNVYAYISIIALIVCIPPAVIIEG
PQLLQHGFNDAIAKVGLTKFVSDLFFVGLFYHLYNQVATNTLERVAPLTHAVGNVLKRVFVIGF
SIIVFGNRITTQTGIGTCIAIAGVAIYSYIKAKIEEEKRVFQWHH*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	7720154-7719246(+) 7720194-7719804(+) 7720219-7720207(-)
Potential amino acid sequence	MLEIIALMKPVQLNESSVSDAIETPFSPLRS(+) MKVQSVMPLKHPFLLFDLSLDVRVDGNTSYGNASTNSSLCGNSVAKNNDGESNDENPFQHIANG MRQRGHSLQSVCSNLVIEVVEQSHEEKVRNELCQAYFCNCIVKTMLQ(+) MASLTELSFNWTGFINAMISNISFTYRSIYSKKAMTDMDSTNVYAYISIIALIVCIPPAVIIEG PQLLQHGFNDAIAKVGLTKFVSDLFFVGLFYHLYNQVATNTLERVAPLTHAVGNVLKRVFVIGF SIIVFGNRITTQTGIGTCIAIAGVAIYSYIKAKIEEEKRVFQWHH*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os01g0239100_circ_g.1

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